Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,761,889	C→A	D388Y (GAT→TAT)	folC ←	tetrahydrofolate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,761,889	0	C	A	100.0%	72.2 / NA	24	D388Y (GAT→TAT)	folC	tetrahydrofolate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (17/7);  total (17/7)

TCATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGTTTT  >  CP000730/1761756‑1762025                                                                                                                                      |                                                                                                                                         tcATGGCTATTTTAGTTTTAGTTTTATAATGCCTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAAt                                                                                                                                   >  1:356485/1‑141 (MQ=255)       cTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCACCGTAGTCATATACTAATTGTAtt                                                                                                                             >  1:409438/1‑141 (MQ=255)                  ttagttttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTc                                                                                                                  <  1:34848/141‑1 (MQ=255)                  ttagttttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCAACATTTTc                                                                                                                  <  1:312249/141‑1 (MQ=255)                    tgttttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCaa                                                                                                                <  1:460196/140‑1 (MQ=255)                                       gTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATAGACTAATGGTATTTCATCATTTTCAATACTACCATCCATgtcttc                                                                                             >  1:286083/1‑141 (MQ=255)                                          tAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCttt                                                                                          >  1:350856/1‑141 (MQ=255)                                             ttttGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCg                                                                                       >  1:403483/1‑141 (MQ=255)                                                 gATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTatc                                                                                   >  1:344074/1‑139 (MQ=255)                                                   tttAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATGATTTTTTATAAATTCAACGTAGTCAGATACTCATTGTCTTATACCGTTTTGAATACTACTTAAAATTTCTTCTTTGCGTAACGc                                                                                 >  1:213208/1‑141 (MQ=255)                                                       aCTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATCCTACCTACAATCTCTTCTTTGCGTAACGCTTTc                                                                             >  1:143703/1‑141 (MQ=255)                                                       aCTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTc                                                                             >  1:23118/1‑141 (MQ=255)                                                       aCTTCACTTATGAAATACAGACTACCGGCAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTTGGTAACGCTTTc                                                                             >  1:320887/1‑141 (MQ=255)                                                               tATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTGTTTGCGTAACGCTTTCGGAAAATc                                                                     >  1:37291/1‑141 (MQ=255)                                                                                cGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATaaa                                                    <  1:212060/141‑1 (MQ=255)                                                                                aagcaaatattAATGTATCAACTTGATAATTTTTTATAAATTCAACCTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATaaa                                                    <  1:89493/131‑1 (MQ=255)                                                                                   tAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACCACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACg                                                 <  1:348140/141‑1 (MQ=255)                                                                                                                 ttATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCACTTAAACTGAAATGTTTGACCa                   >  1:71790/1‑141 (MQ=255)                                                                                                                 ttATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCa                   >  1:251307/1‑141 (MQ=255)                                                                                                                 ttATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCa                   >  1:102951/1‑141 (MQ=255)                                                                                                                    taAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCAtct                <  1:189377/141‑1 (MQ=255)                                                                                                                            aCGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTaacc        >  1:268474/1‑139 (MQ=255)                                                                                                                              gTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCgg      >  1:103166/1‑141 (MQ=255)                                                                                                                                  tCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTTCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAACACGTAGCCGCAATTAAACTTCAATGTTTGGCCACCCCGTTAATGCGtttt  >  1:6156/1‑141 (MQ=255)                                                                                                                                      |                                                                                                                                         TCATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGTTTT  >  CP000730/1761756‑1762025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑