Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,184,430	G→T	T176K (ACA→AAA)	yhgF ←	S1 domain RNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,184,430	0	G	T	100.0%	62.3 / NA	19	T176K (ACA→AAA)	yhgF	S1 domain RNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (8/11);  total (8/11)

AAACTCTATGATTAGCAATGCGTTTAATTGGCTCACTATATGCATAGTACATTTCAAAAATACCTTTTTCATCTTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTGTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAAATTGTTGTGCTTTTTCTTCAATTGAGACTTCATGTTTACGTGC  >  CP000730/2184293‑2184566                                                                                                                                          |                                                                                                                                         aacccctATGATTAGCAATGCGTTTAATTGGATCACTATATGCATAGTACATTTCAAAAATACCTTTTTCATCTTCAGCATTTTTCGTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTc                                                                                                                                       <  1:29549/136‑1 (MQ=255)                     cGTTTAATTGGCTCACTATATGCATAGTACATTTCAAAAATACCTTTTTCATCTTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGaa                                                                                                                   <  1:391152/141‑1 (MQ=255)                                    cTATATGCATAGTACATTTCAAAAATACCTTTTTCATCCTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAACTTTGTTCCGCaata                                                                                                    <  1:323922/141‑1 (MQ=255)                                            aTAGTACATTTCAAAAATACCTTTTTCATCTTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTtg                                                                                            <  1:482099/141‑1 (MQ=255)                                            aTAGTACATTTCAAAAATACCTTTTTCATCTTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTtg                                                                                            <  1:138917/141‑1 (MQ=255)                                                     ttCAAAAATACCTTTTTCATCTTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCttt                                                                                   >  1:415590/1‑141 (MQ=255)                                                              aCCTTTTTCATCTTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTTTGCACCTTTGATAGCATc                                                                          <  1:494556/141‑1 (MQ=255)                                                               ccTTTTTCATCTTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCt                                                                         >  1:62094/1‑141 (MQ=255)                                                                        tCTTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGGTGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCATTAATGTCTTGTGCACCTTTGATAGCCTCTTCAACCGAt                                                                >  1:183375/1‑141 (MQ=255)                                                                         cTTCAGCATTTTTCTATTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGAtt                                                               >  1:368564/1‑141 (MQ=255)                                                                           tCAGCATTTTTCTTTCTATATGCAGCTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGc                                                             <  1:133870/141‑1 (MQ=255)                                                                              gCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCAct                                                          <  1:263316/141‑1 (MQ=255)                                                                                 tttttCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCAattct                                                       >  1:105598/1‑141 (MQ=255)                                                                                                gTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCTACCGATTGCACTTCTTCATTtattagtggt                                        >  1:125137/1‑134 (MQ=255)                                                                                                   gTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAAATtgttgt                                     <  1:216509/141‑1 (MQ=255)                                                                                                         acacCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATGATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGTACTTCCTCATTTATAAATTGTTGTGCtttt                               >  1:62084/1‑141 (MQ=255)                                                                                                         acacCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGAAGTAGCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAAATTGTTGTGCtttt                               >  1:387860/1‑141 (MQ=255)                                                                                                                           tCTTTTAAATTTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAAATTGTTGTGCTTTTTCTTCAATTGAGACTTCa             <  1:210590/141‑1 (MQ=255)                                                                                                                           tCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAAATTGTTGTGCTTTTTCTTCAATTGAGACTTCa             <  1:44139/141‑1 (MQ=255)                                                                                                                                      ttttttTCTATATTTAGGTTTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATTAGTTGTTGTGCTTTTTCTTCAATTGAGACTTCATGTTTACGTGc  >  1:103431/1‑141 (MQ=255)                                                                                                                                          |                                                                                                                                         AAACTCTATGATTAGCAATGCGTTTAATTGGCTCACTATATGCATAGTACATTTCAAAAATACCTTTTTCATCTTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTGTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAAATTGTTGTGCTTTTTCTTCAATTGAGACTTCATGTTTACGTGC  >  CP000730/2184293‑2184566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑