Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,236,856	A→T	L308I (TTA→ATA)	rho ←	transcription termination factor Rho

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,236,856	0	A	T	100.0%	50.7 / NA	20	L308I (TTA→ATA)	rho	transcription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (8/12);  total (8/12)

TCCTTTAAATTCTTCGTAAATCATATCGTCCATACGTGAACCCGTATCAACTAATGCAGTTGCAAGTATTGTTAAACTTCCACCCGCTTCAATATTTCTCGCTGCACCGAAGAATGCTTTTGGTTTGTGTAAAGATGCAGGATCTAAACCACCTGATAATGTACGACCACTTGGTGGAATAACTAAGTTATAAGCGCGTGCTAATCTCGTTATAGAATCCATTAAAATAATGACATCTTCCCCAATTTCTACTAAACGCTTTG  >  CP000730/2236725‑2236987                                                                                                                                    |                                                                                                                                    tCCTTTAAATTCTTCGTAAATCATATCGTCCATACGTGAACCCGTATCAACTAATGCAGTTGCAAGTATTGTTAAACTTCCACCCGCTTCAATATTTCTCGCTGCACCGAAGAATGCTTTTGGTTTGTGTATAGATGCAgg                                                                                                                            >  1:388942/1‑141 (MQ=255)        aaTTCTTCGGAAATCATATCGTCCATACGTGAACCCGTATCAACTAATGCAGTTGCAAGTATTGTTAAACTTCCACCCGCTTCAATATTTCTCGCTGCACCGAAGAATGCTTTTGGTTTGTGTATAGATGCAGGATCTaaa                                                                                                                     <  1:461475/141‑1 (MQ=255)           tcttcCTAAATCATATCGTCCATACGTGAACCCGTATCAACTAATGCAGTTGCAAGTATTGTTAAACTTCCACCCGCTTCAATATTTCTCGCTGCACCGAAGAATGCTTTTGGTTTGTGTATAGATGCAGGATCTAAacca                                                                                                                  <  1:393646/141‑1 (MQ=255)                               cATCCGTGAACCCGTATCAACTAATGCAGTTGCAAGTATCGTTAAACTTCCACCCGCCTCAATATTTCTCGCTGCACCGAAGAATGCTTTTGGTTTGTGTATAGATGCAGGATCTAAACCACCTGATAATGTACGACCACt                                                                                              <  1:330597/141‑1 (MQ=255)                                   cGTGAACCCGTATCAACTAATGCAGTTGCAAGTATTGTTAAACTTCCACCCGCTTCAATATTTCTCGCTGCACCGAAGAATGCTTTTGGTTTGTGTATAGATGCAGGATCTAAACCACCTGATAATGTACGACCACTtggt                                                                                          >  1:382784/1‑141 (MQ=255)                                        aCCCGTATCAACTAATGCAGTTGCAAGTATTGTTAAACTTCCACCCGCTTCAATATTTCTCGCTGCACCGAAGAATGCTTTTGGTTTGTGTATAGATGCAGGATCTAAACCACCTGATAATGTACGACCACTTGGTGGAAt                                                                                     <  1:166546/141‑1 (MQ=255)                                        aCCCGTATCAACTAATGCAGTTGCAAGTATTGTTAAACTTCCACCCGCTTCAATATTTCTCGCTGCACCGAAGAATGCTTTTGGTTTGTGTATAGATGCAGGATCTAAACCACCTGATAATGTACGACCACTTGGTGGAAt                                                                                     <  1:182626/141‑1 (MQ=255)                                            gTATCAACTAATGCAGTTGCAAGTATTGTTAAACTTCCACCCGCTTCAATATTTCTCGCTGCACCGAAGAATGCTTTTGGTTTGTGTATAGATGCAGGATCTAAACCACCTGATAATGTACGACCACTTGGTGGGATAACt                                                                                 >  1:2134/1‑141 (MQ=255)                                                    tAATGCAGTTGCAAGTATTGTTAAACTTCCACCCGCTTCAATATTTCTCGCTGCACCGAAGAATGCTTTTGGTTTGTGTATAGATGCAGGATCTAAACCACCTGATAATGTACGACCACTTGGTGGAATAACTAAGTtata                                                                         <  1:67742/141‑1 (MQ=255)                                                            ttGCAAGTATTGTTAAACTTCCACCCGCTTCAATATTTCTCGCTGCACCGAAGAATGCTTTTGGTTTGTGTATAGATGCAGGATCTAAACCACCTGATAATGTACGACCACTTGGTGGAATAACTAAGTTATAAGCGCGTg                                                                 <  1:243748/141‑1 (MQ=255)                                                            ttGCAAGTATTGTTAAACTTCCACCCGCTTCAATATTTCTCGCTGCACCGAAGAATGCTTTTGGTTTGTGTATAGATGCAGGATCTAAACCACCTGATAATGTACGACCACTTGGTGGAATAACTAAGTTATAAGCGCGTg                                                                 <  1:473766/141‑1 (MQ=255)                                                               cAAGTATTGTTAAACTTCCACCCGCTTCAATATTTCTCGCTGCACCGAAGAATGCTTTTGGTTTGAGTATAGATGCAGGATCTAAACCCATTGTAAATTTACTTTGAAATGGAGGAATAAGTAAGTTATAAACGCGGGCTa                                                              >  1:233818/1‑141 (MQ=255)                                                                           aaCTTCCACCCGCTTCAATATTTCTCGCTGCACCGAAGAATGCTTTTGGTTTGTGTATAGATGCAGGATCTAAACCACCTGATAATGTACGACCACTTGGTGGAATAACTAAGTTATAAGCGCGTGCTAATCTCGTTATAg                                                  >  1:192556/1‑141 (MQ=255)                                                                                ccACCCGCTTCAATATTTCTCGCTGCACCGAAGAATGCTTTTGGTTTGTGTATAGATGCAGGATCTAAACCACCTGATAATGTACGACCACTTGGTGGAATAACTAAGTTATAAGCGCGTGCTGATCTCGTTATAGAATcg                                             >  1:144112/1‑140 (MQ=255)                                                                                    ccGCTTCAATATTTCTCGCTGCACCGAAGAATGCTTTTGGTTTGTGTATAGATGCAGGATCTAAACCACCTGATAATGTACGACCACTTGGTGGAATAACTAAGTTATAAGCGCGTGCTAATCTCGTTATAGAATCCATTa                                         >  1:151308/1‑141 (MQ=255)                                                                                         tCAATATTTCTCGCTGCACCGAAGAATGCTTTTGGTTTATGTATAGATGCAGGATCTAAACCACCTGATAATGTACGACCACTTGGAGGAATAACTAAGTTATAAGCGCGTGCTAATCTCGATATAGAATCCATTAAaata                                    <  1:323067/141‑1 (MQ=255)                                                                                          cAATATTTCTCGCTGCACCGAAGAATGCTTTTCGTTTGTGTATAGATGCAGGATCTAAACCAGCTGATAATGTACGACCACTTGGTGGAATAACTAAGTTATAAGCGCGTGCTAATCTCGTTATAGAATCCATTAAaataa                                   >  1:356769/1‑141 (MQ=255)                                                                                                       tGCACCGAAGAATGCTTTTGGTTTGTGTATAGATGCAGGATCTAAACCACCTGATAATGTACGACCACTTGGTGGAATAACTAAGTTATAAGCGCGTGCTAATCTCGTTATAGAATCCATTAAAATAATGACATCTTcccc                      <  1:12360/141‑1 (MQ=255)                                                                                                                    gCTTTTGGTTTGTGTATAGATGCAGGATCTAAACCACCTGATAATGTACGACCACTTGGTGGAATAACTAAGTTATAAGCGCGTGCTAATCTCGTTATAGAATCCATTAAAATAATGACATCTTCCCCAATTTCTACTaaa         <  1:275118/141‑1 (MQ=255)                                                                                                                           gTTTGTGTATAGATGCAGGATCTAAACCACCTGATAATGTACGACCACTTGGTGGAATAACTAAGTTATAAGCGCGTGCTAATCTCGTTATAGAATCCATTAAAATAATGACATCTTCCCCAATTTCTACTAAACGCTTTg  <  1:214691/141‑1 (MQ=255)                                                                                                                                    |                                                                                                                                    TCCTTTAAATTCTTCGTAAATCATATCGTCCATACGTGAACCCGTATCAACTAATGCAGTTGCAAGTATTGTTAAACTTCCACCCGCTTCAATATTTCTCGCTGCACCGAAGAATGCTTTTGGTTTGTGTAAAGATGCAGGATCTAAACCACCTGATAATGTACGACCACTTGGTGGAATAACTAAGTTATAAGCGCGTGCTAATCTCGTTATAGAATCCATTAAAATAATGACATCTTCCCCAATTTCTACTAAACGCTTTG  >  CP000730/2236725‑2236987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑