Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,279,705	G→A	intergenic (+1373/+6659)	mtlD → / ← glmM	mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,279,705	0	G	A	100.0%	115.5 / NA	38	intergenic (+1373/+6659)	mtlD/glmM	mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/21);  total (17/21)

GTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGC  >  CP000730/2279567‑2279838                                                                                                                                           |                                                                                                                                      gTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACt                                                                                                                                     >  1:63488/1‑141 (MQ=255)  ttGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtg                                                                                                                                    >  1:339828/1‑141 (MQ=255)                gTTGATTCTACTTCTGGTTTACTAGTTACATAGTTATCCATTGTCGGACTGTTTGTTGATGCATCTAAACTAGAATTGTTATTAGCTTGTTTGTTATCATTTGCATCATCAATTGCTGATGTTACTGTTGTTTCACCTGtt                                                                                                                      >  1:161528/1‑141 (MQ=255)                     ttCTACTTCTGGTTTACTAGTTATATCGGTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGc                                                                                                                 <  1:160454/141‑1 (MQ=255)                      tCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCa                                                                                                                <  1:203089/141‑1 (MQ=255)                        tACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATc                                                                                                              >  1:439746/1‑141 (MQ=255)                         aCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCa                                                                                                             >  1:327621/1‑141 (MQ=255)                               ggTTTACTAGTTACATCGTCATCCATTGTCGGACTGTTTGTTGATGCCTCTACACTAGAATTGCTATTAGCTTGCGGTTTATCATTTGCATCAGCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtattt                                                                                                       >  1:264174/1‑140 (MQ=255)                                   tACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTg                                                                                                   <  1:55750/141‑1 (MQ=255)                                    aCTAGTTACATCGTTATCCATTGTCGGACTGTTTGGTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTgg                                                                                                  <  1:333568/141‑1 (MQ=255)                                        gTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGtgtt                                                                                              <  1:178589/141‑1 (MQ=255)                                               cGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGgag                                                                                       >  1:139295/1‑141 (MQ=255)                                                 ttATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTCTTATTTGGTGTTGTCggcggc                                                                                     >  1:177040/1‑137 (MQ=255)                                                   aTCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTACTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGc                                                                                   <  1:337474/141‑1 (MQ=255)                                                     ccATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGt                                                                                 >  1:438235/1‑141 (MQ=255)                                                          gTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCCCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCt                                                                            >  1:149343/1‑141 (MQ=255)                                                          gTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtat                                                                                                         <  1:56594/112‑1 (MQ=255)                                                             ggACCGTTTGTTGATGCATCTACACTAGAATTATTATTATCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGGTACTGTTGTCTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTg                                                                         <  1:332247/141‑1 (MQ=255)                                                                cTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCa                                                                      <  1:306269/141‑1 (MQ=255)                                                                cTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTAATTGGTGTTGTCGGAGAAGCGTCTCCTTTGCCa                                                                      >  1:475012/1‑141 (MQ=255)                                                                   tttgttGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTa                                                                   >  1:191064/1‑141 (MQ=255)                                                                    ttgttgATGCATCTCCACTAGTATTGTTATTAGCTTGCGGTTTATCAGTTGCAGCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAg                                                                  <  1:416832/141‑1 (MQ=255)                                                                    ttgttgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCCTCATCAGTTGCTGATGTTACTGTTGTTTCACATGTTGCAGAATCAATCACATTCGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAg                                                                  >  1:222819/1‑141 (MQ=255)                                                                      gttgATGCACCTACACTAGAATTGGTATTAGCTTGCGGTTTATCATCTGCATCATCAGTTGCTGTGGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTCGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCt                                                                <  1:478093/141‑1 (MQ=255)                                                                           tGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTgtcgt                                                           <  1:180492/141‑1 (MQ=255)                                                                           tGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTCACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTgtcgt                                                           <  1:335613/141‑1 (MQ=255)                                                                                      tAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGt                                                <  1:440292/141‑1 (MQ=255)                                                                                       aGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAACCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGtt                                               >  1:463488/1‑141 (MQ=255)                                                                                        gAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTGAGATACGTTa                                              <  1:360301/141‑1 (MQ=255)                                                                                               tattaGCTTGCGGTTTATAATTTGCATCATCAGGTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTc                                       <  1:83163/141‑1 (MQ=255)                                                                                                    gCTTGCGGTTTATCATTTGCATCAGCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTa                                  <  1:313526/141‑1 (MQ=255)                                                                                                       cgCGGTTTATCATTTACATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTATCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTtttt                               <  1:357274/140‑1 (MQ=255)                                                                                                           gTTTATCCTTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTCGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTg                           <  1:354697/141‑1 (MQ=255)                                                                                                                tCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGtt                      >  1:435391/1‑141 (MQ=255)                                                                                                                tCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAGTTTCTGGTGtt                      >  1:435418/1‑141 (MQ=255)                                                                                                                     tGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCAtt                 >  1:395013/1‑141 (MQ=255)                                                                                                                            tcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGAAttt          <  1:337378/141‑1 (MQ=255)                                                                                                                                    tGATGTTACTGTTGTCTCACCTGTTGACGCATCAATATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCACATACGTTTGGTTGTCCAGTATTTTCTGGTGTCGCATTAGCATTTGAATTTGc  <  1:302590/141‑1 (MQ=255)                                                                                                                                           |                                                                                                                                      GTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGC  >  CP000730/2279567‑2279838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑