Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,352,815	G→A	A92V (GCA→GTA)	rpsI ←	ribosomal protein S9

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,352,815	0	G	A	100.0%	77.3 / NA	26	A92V (GCA→GTA)	rpsI	ribosomal protein S9
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/16);  total (10/16)

TCCGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGT  >  CP000730/2352683‑2352944                                                                                                                                     |                                                                                                                                  tCCGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATAAAGGGAGTGGGAAAGAACCTCTGTATTCAGGATCTACTTCtaat                                                                                                                           >  1:257023/1‑141 (MQ=37)       aaTTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTCATCCGGCGCGTTTTAAAGAACCTCTGTATTAAGGATCTACTTCTAATAATGCa                                                                                                                     >  1:213841/1‑141 (MQ=255)        aTTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCAc                                                                                                                    >  1:456636/1‑141 (MQ=255)                 ttGAGAATTGAGGTGAACGACGCGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTAATACGTAGGTCACGAGTAAGTAATCCAGCGCGTTTGAAAGAAGCTCTGCATTCAGGATCTACTTCTAATAATGCACGAGCGATTc                                                                                                           <  1:344018/141‑1 (MQ=38)                     gaATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATAGAGGATGTACTTCTAATAATGCACGAGCGATTCCGTg                                                                                                       >  1:336225/1‑141 (MQ=255)                     gaATTGAGGTGAACGACGAGCTCACCTAATGCCTGGTTATTTCTGACCGGCGGTTCGGGTGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTg                                                                                                       <  1:359333/141‑1 (MQ=255)                        ttGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACg                                                                                                    >  1:354387/1‑141 (MQ=255)                            ggTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATa                                                                                                >  1:248392/1‑141 (MQ=255)                                         ctgctTTAAGACCTTGTTTTTTACGTTCCTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTc                                                                                   <  1:211081/141‑1 (MQ=255)                                             tttAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGGGCTTGTCCAgg                                                                               >  1:180334/1‑140 (MQ=255)                                                     cTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATTAAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAacccc                                                                       >  1:341798/1‑139 (MQ=255)                                                         ttttttACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGGGCTTGTCCAGTGAAaccaccacc                                                                   >  1:232679/1‑141 (MQ=255)                                                           ttttACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCAt                                                                 <  1:278470/141‑1 (MQ=255)                                                           ttttACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAACGCTCTGCCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCCCCACCAt                                                                 >  1:13975/1‑141 (MQ=255)                                                             ttACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGa                                                               <  1:218520/141‑1 (MQ=255)                                                             ttACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGa                                                               <  1:352509/141‑1 (MQ=255)                                                                                ggTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTACTTCTAATAATGTACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAAcatcat                                            <  1:344885/141‑1 (MQ=255)                                                                                      caggaagtaaTCCATCGCGTTTTAAAGAACCTTTGTATTCAGGATCTACTACTCCTAAAGCACTAGGGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTAc                                      <  1:235766/137‑1 (MQ=255)                                                                                        gtaaggaaTCCAGCGCGTTTTGAAGAACCTCTGTATTCAGGATCTACTTCGAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCt                                    <  1:177/141‑1 (MQ=255)                                                                                                         ttttAAAGAACCTCTGTATTCAGGATCTACTTCCAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCCCCCCAAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATc                   <  1:469331/141‑1 (MQ=255)                                                                                                            tAAAGAACCTCTGTATTCAGGATCTACTTCTAATAACGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCaaa                <  1:203351/141‑1 (MQ=255)                                                                                                               aGAACCTCTGTATTCAGGATCTACTTCTAATAATGCCAGTGAGGTTGCGGGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAAtgg             <  1:257490/141‑1 (MQ=255)                                                                                                                gAACCTCTGTATTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAAtggt            <  1:398154/141‑1 (MQ=255)                                                                                                                          tttCGGGATCTACTTCTAATAATGCACGGGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGt  <  1:40115/140‑1 (MQ=255)                                                                                                                          aTTCCGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGt  <  1:154016/141‑1 (MQ=255)                                                                                                                          aTTCAGGATCTACTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGt  <  1:320078/141‑1 (MQ=255)                                                                                                                                     |                                                                                                                                  TCCGACAATTAACGTTTTGAGAATTGAGGTGAACGACGAGCTGCTTTAAGACCTGGTTTTTTACGTTCTTTCATACGTGGGTCACGAGTAAGTAATCCAGCGCGTTTTAAAGAACCTCTGTATTCAGGATCTGCTTCTAATAATGCACGAGCGATTCCGTGACGGATAGCTTGAGCTTGTCCAGTGAAACCACCACCATGAACGTTAACTAAAACATCATAGTTACCTTTAGTTTCAGTTACATCAAATGGTTGGTTTAAGT  >  CP000730/2352683‑2352944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑