| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP000730 | 134,393 | G→A | L12L (TTG→TTA) | sbnA → | possible pyridoxal‑phosphate dependent enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 134,393 | 0 | G | A | 96.7% | 102.7 / NA | 30 | L12L (TTG→TTA) | sbnA | possible pyridoxal‑phosphate dependent enzyme |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (10/19); minor base C (1/0); total (11/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATGCATAAAAAATATAATCCTAATTACTTGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTGTTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGATCGACCTGCCAAGTAC > CP000730/134257‑134516 | aaTGCATAAAAAATATAATCCTAATTACTTGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAg < 1:307005/141‑1 (MQ=255)aaTGCATAAAAAATATAATCCTAATTACTTGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAg < 1:379630/141‑1 (MQ=255) tGCATAAAAAATATAATCCTAATTACTTGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGAt < 1:346155/141‑1 (MQ=255) cATAAAAAATATAATCCTAATTACTTGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTc < 1:403465/141‑1 (MQ=255) tAAAAAATATAATCCTAATTACTTGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTAATTATTAGATTCTg > 1:305926/1‑141 (MQ=255) tAATTACTTGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTa < 1:126412/141‑1 (MQ=255) aTAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACt < 1:127869/141‑1 (MQ=255) gagaATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTtcatc < 1:228557/141‑1 (MQ=255) gagaATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTtcatc < 1:431808/141‑1 (MQ=255) agaATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTtcatca < 1:410440/141‑1 (MQ=255) agaATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTtcatca < 1:380746/141‑1 (MQ=255) aaTCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAAc < 1:96343/141‑1 (MQ=255) aTTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGaaa > 1:350984/1‑141 (MQ=255) aGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACAt < 1:346424/141‑1 (MQ=255) tAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATGGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGaa > 1:253380/1‑141 (MQ=255) attatAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGAGTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCaaa > 1:206765/1‑141 (MQ=255) acttttAAATTTGAGGAGGAAGCGCTGTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTagag < 1:172287/139‑1 (MQ=255) tAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTCTTAGATTCTGTAGGGCAAACACCTATGGTGTTACGTCATCAACTATTTCCGAAACATGAAGGGGTTGCAAAGTTAGAGTatat > 1:385519/1‑141 (MQ=255) aaaTTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATg > 1:374317/1‑141 (MQ=255) aaaTTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATg < 1:389190/141‑1 (MQ=255) aaaTTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCACCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATg < 1:140154/141‑1 (MQ=255) aTTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCGAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGaa > 1:315420/1‑141 (MQ=255) gcgcTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGgcagc > 1:157018/1‑141 (MQ=255) gcgcTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACAACTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGgcagc > 1:439828/1‑141 (MQ=255) cTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATg < 1:304005/141‑1 (MQ=255) gattgaAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGa < 1:256246/141‑1 (MQ=255) ttgaAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAGCTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGGAAGATc > 1:84566/1‑141 (MQ=255) ttgaAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGATc > 1:171650/1‑141 (MQ=255) gTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGATCGACCTGCCa < 1:480715/141‑1 (MQ=255) gCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTAAAATACCCATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGATCGACCTGCCAAGTAc < 1:488726/141‑1 (MQ=255) | AATGCATAAAAAATATAATCCTAATTACTTGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTGTTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGATCGACCTGCCAAGTAC > CP000730/134257‑134516 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |