Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	629,681	C→A	L84I (CTA→ATA)	hxlA →	possible 3‑hexulose‑6‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	629,681	0	C	A	100.0%	59.2 / NA	19	L84I (CTA→ATA)	hxlA	possible 3‑hexulose‑6‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (8/11);  total (8/11)

CGAAGGTTTACCAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGCTGAT  >  CP000730/629545‑629818                                                                                                                                         |                                                                                                                                          cGAAGGTTCACCAGCAGTAAAACAAACGGAAGACAACACGAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGAATATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAgg                                                                                                                                       <  1:90684/141‑1 (MQ=255)                                     caTTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTga                                                                                                   <  1:43144/141‑1 (MQ=255)                                            aaTGTAAAAGTATTAGCAGAAATGAAAATTATAGATGCAGCTGTTTATGAAGTTAGCCAAGAAATTAAATTTGGCGCGGATGTAATTATAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGTTGAAGcg                                                                                            >  1:50385/1‑140 (MQ=255)                                                  aaaGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATaaa                                                                                      >  1:63589/1‑141 (MQ=255)                                                  aaaGTATCAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATaaa                                                                                      <  1:63949/141‑1 (MQ=255)                                                    aGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATaaaaa                                                                                    >  1:103194/1‑141 (MQ=255)                                                    aGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATaaaaa                                                                                    >  1:107035/1‑141 (MQ=255)                                                    aGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGAAGTTAAAGTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATaaaaa                                                                                    >  1:413636/1‑141 (MQ=255)                                                        ttAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGGGTTGCAGAAGAGGCATCAATTCAAGCAGCTATTGAAGAAGCTCATAAAaataat                                                                                >  1:12864/1‑141 (MQ=255)                                                          agcataaaagAAAAATATAGATTCAGTCGATTATGAAGTTAGACAAGGAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAAtaataa                                                                              <  1:30914/132‑1 (MQ=255)                                                          aGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGAAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAAtaataa                                                                              <  1:129047/141‑1 (MQ=255)                                                                    aaaaTTATAGATGCAGCTGAATATGAAGTTAGCCAAGCAATTAAGTTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCACCAATTAAAGCAGCTACTGAAGAAACTCATAAAAATAATAAACAATTACTa                                                                    >  1:301161/1‑141 (MQ=255)                                                                          ataGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGat                                                              <  1:17465/141‑1 (MQ=255)                                                                             gATGCAGATGATTATGAAGTTAGACAAGCAATTAAATTTGGCGAGGATGTAATTACAATAATAGGTGTTAGAGAAGATGCATCATATGCATCTGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATg                                                           <  1:363601/141‑1 (MQ=255)                                                                                  aGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGc                                                      <  1:494645/141‑1 (MQ=255)                                                                                  aGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGc                                                      <  1:14922/141‑1 (MQ=255)                                                                                   gCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCt                                                     <  1:43498/141‑1 (MQ=255)                                                                                    cTGATTATGAAGTTAGACAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTg                                                    <  1:310051/141‑1 (MQ=255)                                                                                                                    ggCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTa                    >  1:438770/1‑141 (MQ=255)                                                                                                                                      ataataGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGCTGAt  <  1:324510/141‑1 (MQ=255)                                                                                                                                         |                                                                                                                                          CGAAGGTTTACCAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGCTGAT  >  CP000730/629545‑629818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑