Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 39,378 C→A 100% D586Y (GAT→TAT)  mecA ← penicillin binding protein 2 prime

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073039,3780CA100.0% 162.8 / ‑6.5 53D586Y (GAT→TAT) mecApenicillin binding protein 2 prime
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (24/28);  total (25/28)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATCTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATATTGCCA  >  CP000730/39241‑39511
                                                                                                                                         |                                                                                                                                     
aaTAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATTATATCTATGAATAtattc                                                                                                                                    >  1:229699/1‑141 (MQ=255)
aaTAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATCTGCCAATTAAGTTTGCATAGGATCTATAAATAtattc                                                                                                                                    >  1:732482/1‑141 (MQ=255)
 aTAGCCATCAACATGTTTGAATTAGCTTTATCATATGATATAAACCACCCAATTCTTATGCCAGTTTCTCCTTGTTTAATTTTGACTTCTGCAGTACCGGCTTTGCCAATTAAGTTTGCATCAAATCTATAAATAtattct                                                                                                                                   >  2:320900/1‑141 (MQ=255)
    gCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTa                                                                                                                                <  1:202767/141‑1 (MQ=255)
     ccattattgtGTTTGGATTCTGTTTAACATATGATAGAAGTCACCCAATTTGTCTGCCTTGTTCTCCTTGTTTCATTTTTAGGTCTGCAGTACCTGATTTGCCAATTAAGTTTGCATAAGATCTATAAAGATATTCTTTAt                                                                                                                               <  2:154967/132‑1 (MQ=255)
      catgatcatTTTTGGATTATCTTTACCCTATGATATAAACCTCCCAATTTGTCTGCCAGTTGCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTAtg                                                                                                                              <  2:411654/141‑1 (MQ=255)
      catcatcatGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTATGTTTGCATACGATCTTTCCATATATTCTTTAtg                                                                                                                              >  2:365362/1‑141 (MQ=255)
      catcatcatGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAACTATATTCTTTAtg                                                                                                                              >  2:365208/1‑141 (MQ=255)
       atgatcatGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTAtgt                                                                                                                             <  1:722758/138‑1 (MQ=255)
        tcatcatGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTAtgtg                                                                                                                            <  1:320259/141‑1 (MQ=255)
         cttcatTTTTAGAGTATCGTTATCATAGGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCGGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATgtgt                                                                                                                           <  2:707976/139‑1 (MQ=255)
           tcatGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGAATATGGGCTAGAAATATATTCTTTATGTGttt                                                                                                                         <  1:559160/141‑1 (MQ=255)
              tGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTtttat                                                                                                                      <  2:589372/141‑1 (MQ=255)
                 ttGGGTTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTGGCATAAGATCTATAAATATATTCTTTATGTGTtttattta                                                                                                                   <  2:633985/141‑1 (MQ=255)
                              ttCAGATGATATAAACCACCTAATTTGTCTGCCAGTTTTTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTAGAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCAt                                                                                                      <  2:623214/140‑1 (MQ=255)
                                       tataAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGt                                                                                             <  2:602754/141‑1 (MQ=255)
                                         taAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCTGTTa                                                                                           >  2:427015/1‑141 (MQ=255)
                                             ccaccCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAg                                                                                       <  2:584186/141‑1 (MQ=255)
                                             ccaccCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAg                                                                                       >  1:225449/1‑141 (MQ=255)
                                               accCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTACGTTTGCATAACCTCTCTAAATATCTTCTTTATGTGTTTTATTTACGACTTGTTGCATCCCATcgctcactcgat                                                                                     >  2:396260/1‑130 (MQ=255)
                                               accCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTCAGTGTTTTCTTTACGACTTGTTGCATACCATCAGTTAATAGAt                                                                                     >  1:710116/1‑141 (MQ=255)
                                               accCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGAt                                                                                     >  1:579475/1‑141 (MQ=255)
                                                     tttGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGatat                                                                               >  1:296226/1‑141 (MQ=255)
                                                         tCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTc                                                                           >  1:43824/1‑141 (MQ=255)
                                                          cTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGTTCGATAAAGATATTCTTTATTTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCt                                                                          <  1:751631/141‑1 (MQ=255)
                                                                    ctcCTTGTTTCGTTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAaatat                                                                >  1:238912/1‑140 (MQ=255)
                                                                    ctcCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAaataa                                                                >  1:271410/1‑141 (MQ=255)
                                                                     tcCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAaataat                                                               <  1:762178/141‑1 (MQ=255)
                                                                     tcCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAaataat                                                               <  2:441559/141‑1 (MQ=255)
                                                                                ttttGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTcc                                                    <  2:259648/141‑1 (MQ=255)
                                                                                 tttGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAATATCTATAAATATATTCTTTATGTGTTTTATTTACGACCTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCa                                                   <  2:283963/141‑1 (MQ=255)
                                                                                 tttGAGTTCTGCAGGACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCa                                                   <  2:693292/141‑1 (MQ=255)
                                                                                         cTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTTGAAATAATATTTTTCTTCCAAACTTTGt                                           >  1:386808/1‑141 (MQ=255)
                                                                                         cTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGGTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGt                                           >  1:22388/1‑141 (MQ=255)
                                                                                            cAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGtttt                                        >  1:38210/1‑141 (MQ=255)
                                                                                             aGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGttttt                                       <  2:432771/141‑1 (MQ=255)
                                                                                              gTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGCAAATAATATTTTTATTCCAAACTTTGTTATTc                                      >  2:383675/1‑141 (MQ=255)
                                                                                                aCCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCgt                                    >  2:60348/1‑141 (MQ=255)
                                                                                                        tGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTta                            <  1:115549/141‑1 (MQ=255)
                                                                                                        tGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTta                            <  2:413399/141‑1 (MQ=255)
                                                                                                          ccAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCGAAACTTTGTTTTTTGTGTCTTTtaat                          >  2:405019/1‑141 (MQ=255)
                                                                                                          ccAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTtaat                          >  1:753967/1‑141 (MQ=255)
                                                                                                          ccAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTtaat                          >  1:761721/1‑141 (MQ=255)
                                                                                                           cAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTACTAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTtaata                         >  2:627025/1‑141 (MQ=255)
                                                                                                               tAAGTTTGCATAGAATCTATAAATATATTCTTGATGTGTTTTATGTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTg                     <  2:38851/141‑1 (MQ=255)
                                                                                                                  gTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAgg                  <  1:304846/141‑1 (MQ=255)
                                                                                                                   tttGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAgcgcaaa                 >  2:565309/1‑135 (MQ=255)
                                                                                                                   tttGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGt                 <  1:712829/141‑1 (MQ=255)
                                                                                                                    ttGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTg                >  2:238212/1‑141 (MQ=255)
                                                                                                                     tGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGc               >  1:698939/1‑141 (MQ=255)
                                                                                                                          aaGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTaa          <  1:721844/141‑1 (MQ=255)
                                                                                                                             aTCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAatat       >  1:523548/1‑141 (MQ=255)
                                                                                                                              tCTATTAATATATTCTGTAGGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTTCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATAtt      <  2:490927/141‑1 (MQ=255)
                                                                                                                              tCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATAtt      <  2:625244/141‑1 (MQ=255)
                                                                                                                                 ataAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTCATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATATTGcc   <  2:604550/141‑1 (MQ=255)
                                                                                                                                  taAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATATTGCCa  >  1:441772/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                     
AATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATCTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATATTGCCA  >  CP000730/39241‑39511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: