Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,963,768 C→G 100% V365L (GTA→CTA)  hemG ← protoporphyrinogen oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,963,7680CG100.0% 312.6 / ‑6.5 95V365L (GTA→CTA) hemGprotoporphyrinogen oxidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (47/47);  minor base T (0/1);  total (47/48)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGTCT  >  CP000730/1963631‑1963904
                                                                                                                                         |                                                                                                                                        
aCTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATa                                                                                                                                       <  1:658849/141‑1 (MQ=255)
aCTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATa                                                                                                                                       >  1:157523/1‑141 (MQ=255)
 cTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATACATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATAc                                                                                                                                      >  2:520024/1‑141 (MQ=255)
    ttCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCa                                                                                                                                   <  1:409832/141‑1 (MQ=255)
      cGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCCACCACAGTATCACCTGGTTTACCTAGATACGCACg                                                                                                                                 <  2:380224/141‑1 (MQ=255)
      cGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACg                                                                                                                                 <  1:656705/141‑1 (MQ=255)
           aaCGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGCCTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCa                                                                                                                            >  1:711154/1‑141 (MQ=255)
            aCGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCaa                                                                                                                           <  1:698554/141‑1 (MQ=255)
                   aCAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGAGACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGCATCAAAccctta                                                                                                                    >  1:126628/1‑141 (MQ=255)
                   aCAATTGTAAATTCAGGAGCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAacctta                                                                                                                    <  1:320853/141‑1 (MQ=255)
                       ttGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAaccttacctt                                                                                                                >  1:593373/1‑141 (MQ=255)
                          tAAACTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGCACAATCGATACTAATTCATTATCTGTAGGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCg                                                                                                             <  2:172194/141‑1 (MQ=255)
                           aaaTTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCgg                                                                                                            >  1:161134/1‑141 (MQ=255)
                            aaTTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCggg                                                                                                           >  1:229356/1‑140 (MQ=255)
                            aaTTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCgct                                                                                                           >  2:128232/1‑139 (MQ=255)
                            aaTTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGgt                                                                                                           <  2:447313/141‑1 (MQ=255)
                            aaTTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGgt                                                                                                           <  2:161134/141‑1 (MQ=255)
                               tCAGGATCACCTTTAAATGCCATCATTTGACTTAAATCTCTACGCACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTgta                                                                                                        >  1:402984/1‑141 (MQ=255)
                                   gATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGaaa                                                                                                    >  1:273327/1‑141 (MQ=255)
                                        ccTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGc                                                                                               <  1:26182/141‑1 (MQ=255)
                                        ccTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGc                                                                                               >  1:446082/1‑141 (MQ=255)
                                        ccTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATCCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGc                                                                                               <  1:734820/141‑1 (MQ=255)
                                           ttAAATGTCATCATTTGACTTAAATCTCTATGTCCACTCTACACTAACTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAt                                                                                            <  1:586719/141‑1 (MQ=255)
                                           ttAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAt                                                                                            <  2:566224/141‑1 (MQ=255)
                                             aaaTGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAttt                                                                                          >  2:61018/1‑141 (MQ=255)
                                             aaaTGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAttt                                                                                          >  1:234480/1‑141 (MQ=255)
                                              aaTGTCATCATTTGCCTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGCGGTAGTAAATGGCCAtttt                                                                                         >  2:28690/1‑141 (MQ=255)
                                              aaTGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAtttt                                                                                         <  2:444509/141‑1 (MQ=255)
                                                 gTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTc                                                                                      <  1:122241/141‑1 (MQ=255)
                                                  tcatcaTTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCg                                                                                     >  2:164992/1‑141 (MQ=255)
                                                  tcatcaTTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCg                                                                                     >  1:580627/1‑141 (MQ=255)
                                                  tcatcaCTTGACCTAAACCACTACGTACAATCGATAGTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAACTGGCCATTTTTTCg                                                                                     <  1:483814/141‑1 (MQ=255)
                                                   catcatTTGGCATAAATCTCTACATACAATCGAGACTAATTCAGTATCTGTGTAATCATCAACCACAGTATCACCTGGTTTACCTAGATACACACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGa                                                                                    >  2:190542/1‑141 (MQ=255)
                                                   catcatTTGCCTTAAATCTCTACGTACAATCGATACTAATTCATTCTCTGTATGATCATCAACCACAGTATCACCTGTTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGa                                                                                    >  2:382180/1‑141 (MQ=255)
                                                   catcatTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCAGGTGTAGTAAATGGCCATTTTTTCGa                                                                                    >  2:332291/1‑141 (MQ=255)
                                                    atcatTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGAt                                                                                   >  1:572432/1‑141 (MQ=255)
                                                        tttGACTTATATCTCTACGTACAATTGATACTAATTCATTATCTGTATCATCATCAACCACAGTATCACCTGGTTTACCGAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCGTTTTTTCGATGTcc                                                                               <  2:331649/141‑1 (MQ=255)
                                                               tAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACa                                                                        <  1:340911/141‑1 (MQ=255)
                                                               tAAATCTCTACGTACAAGCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTCACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACa                                                                        <  1:405248/141‑1 (MQ=255)
                                                                   tctaTACGTACAATCGATACTAATTCATTATTTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCg                                                                    <  2:258628/141‑1 (MQ=255)
                                                                      ctACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTa                                                                 >  1:407473/1‑141 (MQ=255)
                                                                       tACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCCCCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTCGTAAATGGCCATTTTTTAGCCGTACAAGTAcccgcgttat                                                                <  2:443920/141‑10 (MQ=255)
                                                                       tACGTACAATCGATACTAATTCATTATCTGTATGATACCCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGTGGTaa                                                                <  2:157523/141‑1 (MQ=255)
                                                                         cGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATg                                                              <  2:412867/141‑1 (MQ=255)
                                                                          gTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTCTCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGt                                                             <  2:577446/141‑1 (MQ=255)
                                                                            tcAATCGATCTTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTTCCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCt                                                           <  1:193410/140‑1 (MQ=255)
                                                                            gccaTCGATACTAGTTCATTATCTGTAGGAGCATCAACCACAGTATTACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCt                                                           <  2:717542/138‑1 (MQ=255)
                                                                               aTCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTGGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGGCTGTa                                                        >  2:140332/1‑141 (MQ=255)
                                                                               aTCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTa                                                        >  2:605647/1‑141 (MQ=255)
                                                                               aTCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTa                                                        >  1:418880/1‑141 (MQ=255)
                                                                                tCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCTTTGTCCACGTACATGCGGTAATGTCGGTAt                                                       >  2:506619/1‑141 (MQ=255)
                                                                                tCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCGTCCGGTGTAGTAACTGGCCATTTTTTCGATGTCCAAGCACATGCGGTAATCTCTGTAt                                                       >  2:114301/1‑141 (MQ=255)
                                                                                  gATACTAATTCATTATCTGTATGATCATCAACCACAGTACCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTTCATGCGGTTATGTCTGTATCa                                                     <  1:392919/141‑1 (MQ=255)
                                                                                       tatttCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGt                                                <  2:450590/138‑1 (MQ=255)
                                                                                       tAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGTGGTAATGTCTGTATCACTCGt                                                <  1:392771/141‑1 (MQ=255)
                                                                                        aaTTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTTTTTTTCTTAGTACATGCGGTAATGTCTGTATCACTCGtt                                               >  2:709338/1‑141 (MQ=255)
                                                                                         aTTCATTATCTGTATGCTCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTtc                                              <  1:28618/141‑1 (MQ=255)
                                                                                            cATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCACg                                           <  2:382681/141‑1 (MQ=255)
                                                                                               tATCTGTATGATCATCAACCACAGTATCACCGGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCaa                                        <  2:622544/141‑1 (MQ=255)
                                                                                                aTCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAAt                                       >  1:316276/1‑141 (MQ=255)
                                                                                                aTCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAAt                                       >  1:316310/1‑141 (MQ=255)
                                                                                                 tcagTATGATCATCAACCACAGTATCACCTTGTTTACCTAGATCCGCACGAATCAAAACCTTTCCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTAAATTCCTTTTTTTTTGTATCACTCGTTCTCGCAAtt                                      <  1:365985/138‑1 (MQ=255)
                                                                                                 tCTGTATGATCATCAACCACAGTATCACCTGGTGTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAAtt                                      <  2:171641/141‑1 (MQ=255)
                                                                                                  cTGTATGATCATCAACCACAGTATCACCTGGTTTACCTCTATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCCTTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCCCGCAAGTa                                     <  2:447516/141‑1 (MQ=255)
                                                                                                  cTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTa                                     <  1:61018/141‑1 (MQ=255)
                                                                                                   tGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTAAATGCGGTAATGTCTGTATCACTTGTTCTCGCAATTAc                                    >  1:45414/1‑141 (MQ=255)
                                                                                                    gaaTGATAATCAACCACAGTATCGCATGGTTTACTTAGATACGCACGAATCGAAACCTTACATTCCGGTGTAGTAAGTGGCCATTTGTTCGATATCCAAGTACAAGCGGTAATGTCTGTATCACGCGTTCTCGCAATTCCg                                   >  2:301776/3‑141 (MQ=255)
                                                                                                    gTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACg                                   <  1:133207/141‑1 (MQ=255)
                                                                                                    gTATGATCACCAACCACAGTATCACCTGGTTTACCCAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACg                                   <  2:409030/141‑1 (MQ=255)
                                                                                                      aTGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGaa                                 >  1:643503/1‑141 (MQ=255)
                                                                                                       tGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTTCCTTCCGGTGTAGTACATGGCCATTTTTTCGATTTTCAAGTCCATGCGGTAATGTCTGTATCACTCGTTCTCGAAATTActgag                                >  2:368078/1‑137 (MQ=255)
                                                                                                         atcatcAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATTTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGcc                              >  2:730055/1‑141 (MQ=255)
                                                                                                          tcatcaACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCa                             <  2:711154/141‑1 (MQ=255)
                                                                                                           catcaACCACAGTATCACCTGGTTTACCTAGATACGCACGAACCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATCTCCAACTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAg                            <  1:252840/141‑1 (MQ=255)
                                                                                                             tcaACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGCAGCCAGTa                          >  1:137151/1‑141 (MQ=255)
                                                                                                             tcaACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTa                          >  1:373386/1‑141 (MQ=255)
                                                                                                              caACCACAGTATCACCTGGTTTACCTAGATACGTAAGAATCAAAATCTTAAATTCCGGTGTAGTTAATGGCCATTTTTTCGATGTCCAAGTACTGGCGGTAATGTCTGTATCATTCGTTCTCGCAATTACGAAGCCAGTAc                         >  2:753038/1‑141 (MQ=255)
                                                                                                              caACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCAGGTGTAGTAAATGGCCATGTTTTCGATGCCCAAGTACATGCGGTAATGACTGTATCACTCGTTCTCGCAATTACGAAGCCAGtcc                         >  2:753113/1‑139 (MQ=255)
                                                                                                                     aGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATg                  >  2:337510/1‑141 (MQ=255)
                                                                                                                     aGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATg                  >  2:112218/1‑141 (MQ=255)
                                                                                                                      gTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCCTgg                 >  2:299141/1‑141 (MQ=255)
                                                                                                                       taccACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATggg                <  1:454740/138‑1 (MQ=255)
                                                                                                                       tATCACCTGGTTCACCTAGATACGCCCTAATCAAAACCTTACCTTTCGGTGTAGTAAATGGCCTTTTTTTCGTTTTCCAAGTACAGGGGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATggg                <  1:751751/141‑1 (MQ=255)
                                                                                                                       gaTCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATggg                <  1:179672/140‑1 (MQ=255)
                                                                                                                        aTCACCTGGTTTACCTAGATACGCACGAATCAAATCCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTATGAAGTTAGTACCATCATGGGt               <  1:560495/141‑1 (MQ=255)
                                                                                                                           cccTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTAtt            <  2:424033/140‑1 (MQ=255)
                                                                                                                           aCCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCACGTACATGCGGTAATGTCTGTATCACCCGTTCTCGCAATTACGAAGCCAGTACCATCCTGGGTAtt            >  2:220624/1‑141 (MQ=255)
                                                                                                                                 tttACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAAt      <  1:620745/141‑1 (MQ=255)
                                                                                                                                  ttACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCaaag     >  1:111438/1‑139 (MQ=255)
                                                                                                                                  ttACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATg     <  1:36743/141‑1 (MQ=255)
                                                                                                                                    aCCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTCTTTTCAATGTc   >  1:278241/1‑141 (MQ=255)
                                                                                                                                    aCCTAGATACGCACGAAGCAAACCCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGCATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGTc   <  2:630388/141‑1 (MQ=255)
                                                                                                                                     ccTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCCTCATGGGTATTTTCAATGGCt  >  2:422619/1‑141 (MQ=255)
                                                                                                                                     ccTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGTCt  >  1:452958/1‑141 (MQ=255)
                                                                                                                                     ccTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGTCt  >  1:763721/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                        
ACTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGTCT  >  CP000730/1963631‑1963904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: