Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,171,944 A→G 6.8% N509D (AAT→GAT)  leuA2 → 2‑isopropylmalate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,171,9440AG 6.8% 203.4 / 3.2 69N509D (AAT→GAT) leuA22‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base A (38/26);  new base G (3/2);  total (41/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGAAGTACATGTAAATTTATTGATTGAAGGTAAGACTGTCAATGGCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTG  >  CP000730/2171804‑2172084
                                                                                                                                            |                                                                                                                                            
cAGAAGTACATGTAAATTTATTGATTGAAGGTAAGACTGTCAATGGCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGATGAGAAggtaggta                                                                                                                                              >  1:281893/1‑141 (MQ=255)
  ggaGTACATGTAAATTTATTGATTGAAGGTAAGACTGTCAATGGCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGtaat                                                                                                                                            <  2:752034/139‑1 (MQ=255)
  aaattACATGTAAACTTATTGATTGAAGGTAAGACTGTCAATGGCCTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGtaat                                                                                                                                            <  1:169344/137‑1 (MQ=255)
   aaGTACATGTAAATTTATTGATTGAAGGTAAGACTGTCAATGGCTTTTGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGtaatt                                                                                                                                           <  2:434639/141‑1 (MQ=255)
   aaGTACATGTAAATTTATTGATTGAAGGTAAGAATGTCAATGGCGATGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCTTATGTAGAAGCACATGCTAAATTTGCAGCTGAAAATTTTGAGAAGGTAGGtaatt                                                                                                                                           >  2:403354/1‑141 (MQ=255)
    aGTACATGTAAATTTATTGATTGAAGGTAAGACTGTCAATGGCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGtaatta                                                                                                                                          >  1:541862/1‑141 (MQ=255)
     gTACATGTAAATTTATTGATTGAAGGTAAGACTGTCAATGGCTTTGGTATTGATCATGATATTTTACAAGCCCCATGTAAAACAGACGTAGAAGCACATGCTAAATTTGCAGCTGCAAATGTTGAGAAGGTAGGtaattaa                                                                                                                                         >  1:360812/1‑141 (MQ=255)
       aCATGTAAATTTATTGATTGAAGGTAAGACTGTCAATGGCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTaattaatt                                                                                                                                       <  2:636651/141‑1 (MQ=255)
              aaTTTATTGATTGAAGGTAAGACTGTCAATGGCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACtt                                                                                                                                <  1:554149/141‑1 (MQ=255)
               aTTTATTGATTGAAGGTAAGACTGTCAATGGCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCGCATGCTAAATTTGCGGCTGAAAAGGTGGAGAAGGTAGGTAATTAATTATGACTta                                                                                                                               >  1:632647/1‑141 (MQ=255)
               aTTTATTGATTGAAGGTAAGACTGTCAATGGCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTta                                                                                                                               >  2:331845/1‑141 (MQ=255)
                 ttATTGATTGAAGGTAAGACTGTCAATGGCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTtata                                                                                                                             <  2:437149/141‑1 (MQ=255)
                  tATTGATTGAAGGTAAGACTGTCAATGGCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATaa                                                                                                                            >  1:137396/1‑141 (MQ=255)
                   attgattgAAGGTAAGACTGTCAATGGCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAAc                                                                                                                           <  2:141115/141‑1 (MQ=255)
                   aatgattgaAGGTAAGACTGTCAATGGCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAAc                                                                                                                           <  2:141416/139‑1 (MQ=255)
                    ttgattgaAGGTAAGACTGTCAATGGCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACa                                                                                                                          >  1:88221/1‑141 (MQ=255)
                    ttgattgaAGGTAAGACTGTCAATGGCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACa                                                                                                                          <  1:409593/141‑1 (MQ=255)
                     tgattgaAGGTAAGAGTGTCAATGGATTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAGTTAATTATGACTTATAACAt                                                                                                                         >  1:381378/1‑141 (MQ=255)
                     tgattgaAGGTAAGACTGTCAATGGCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACAt                                                                                                                         >  1:664086/1‑141 (MQ=255)
                        ttgaAGGTAAGACTGTCAATGGCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCCGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACAttgt                                                                                                                      <  2:571149/141‑1 (MQ=255)
                          gaAGGTAAGACTGTCAATGGCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACAttgttg                                                                                                                    >  1:611537/1‑141 (MQ=255)
                            aGGTAAGACTGTCAATGGCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGcc                                                                                                                  <  2:95944/141‑1 (MQ=255)
                               tAAGACTGTCAATGGCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCtc                                                                                                               >  2:626918/1‑140 (MQ=255)
                               tAAGACTGTCAATGGCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTa                                                                                                               >  1:675158/1‑141 (MQ=255)
                                aaGACTGTCAATGGCTTTGGTATTGATCATGATATTTTACACGCCTATTTAAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTAc                                                                                                              <  1:58371/141‑1 (MQ=255)
                                   aCTGTCAATGGCTTTGGTATTGATCATGATATTGTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTg                                                                                                           <  1:209237/141‑1 (MQ=255)
                                    ttGTTAATGGCTTGGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTgg                                                                                                          <  2:562155/140‑1 (MQ=255)
                                       tCAATGGCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGa                                                                                                       <  2:541862/141‑1 (MQ=255)
                                        cAATGGCTTTGGTATTAATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGAt                                                                                                      >  1:450984/1‑141 (MQ=255)
                                            ggCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAGTTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGGCTTATAACATTGTTGCACTACCTGGTGATGGaa                                                                                                  >  2:234285/1‑141 (MQ=255)
                                            ggCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGaa                                                                                                  >  1:507976/1‑141 (MQ=255)
                                              cTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATc                                                                                                >  2:646935/1‑141 (MQ=255)
                                                   gTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTcc                                                                                           <  2:361590/141‑1 (MQ=255)
                                                     aTTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAg                                                                                         >  1:485979/1‑141 (MQ=255)
                                                      ttGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGa                                                                                        <  2:420734/141‑1 (MQ=255)
                                                        gATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGaaa                                                                                      >  2:52606/1‑141 (MQ=255)
                                                        gATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGaaa                                                                                      >  2:36750/1‑141 (MQ=255)
                                                        gATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACCTATAACATTGTTGCCCTACCTGTTCATGGAATCGGTCCGGaaa                                                                                      >  1:368996/1‑141 (MQ=255)
                                                           cATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGTTTAAGTGGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTTCCTGGTGATGGAATCGGTCCAGAAAttt                                                                                   <  2:568576/141‑1 (MQ=255)
                                                            aggATATTTTAAAAACCTCTTATAAAGCATACGTAGAAGCACATGCTAAAGTTGCAGCTGAAAATGTTGAGAAGGTAGGTGATAAATTATGACTAATAAGATTGTTGCCCAACCTAGAGAAGGAATTGGTCCAGAAAgttt                                                                                  >  2:73268/3‑141 (MQ=255)
                                                            aTGATATATTACAAGCCTCTAGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGGCTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAAtttt                                                                                  >  1:475660/1‑141 (MQ=255)
                                                                 atTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACg                                                                             <  1:83948/141‑1 (MQ=255)
                                                                 atTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACg                                                                             >  1:696010/1‑141 (MQ=255)
                                                                  ttttACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCGATACCTGGTAATGGCGTCGATCCAGAAATTTTGAACgg                                                                            >  2:521943/1‑141 (MQ=255)
                                                                   tttACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTAGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTGTAAACCGa                                                                           >  1:166761/1‑141 (MQ=255)
                                                                       cAAGCGTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAGATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTACAGAAATTTTGAACGGGtctc                                                                       >  2:666682/1‑141 (MQ=255)
                                                                           aggctTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGACAATGTTGAGAAGGTAGGTGATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATc                                                                   >  2:92813/4‑141 (MQ=255)
                                                                           aggctTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTGATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATc                                                                   >  2:92486/4‑141 (MQ=255)
                                                                             tctTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCAt                                                                 <  1:331845/141‑1 (MQ=255)
                                                                                 gTAAAGCATACTTAGAAGCACATGCTAAATGTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTTATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCt                                                             <  1:402481/141‑1 (MQ=255)
                                                                                   aaaGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTg                                                           <  1:98399/141‑1 (MQ=255)
                                                                                     aGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGaa                                                         >  2:697041/1‑141 (MQ=255)
                                                                                       cctACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAAt                                                       <  1:36312/139‑1 (MQ=255)
                                                                                          aCGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATtat                                                    >  1:575594/1‑141 (MQ=255)
                                                                                            gTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATaa                                                  >  2:250536/1‑141 (MQ=255)
                                                                                                aaGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGtaa                                              <  2:267830/141‑1 (MQ=255)
                                                                                                aaGCACATGCTAAATTTGCAGCTGAAAACGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGACCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGtaa                                              <  1:368408/141‑1 (MQ=255)
                                                                                                aaGCACATGCTAAATTTGCAGCTGAAAAAGTTGAGAAGGGAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGAACCAGAAATTTTGAACAGATCTCTATCATTGCTTGAAATTATAAGtaa                                              >  2:493303/1‑141 (MQ=255)
                                                                                                     caTGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTGTAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTaataaat                                         >  1:505478/1‑141 (MQ=255)
                                                                                                      aTGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATACGTaataaaca                                        >  2:720168/1‑139 (MQ=255)
                                                                                                         cTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATaa                                     >  1:295072/1‑141 (MQ=255)
                                                                                                            aaTTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACtt                                  >  2:12065/1‑141 (MQ=255)
                                                                                                               ttGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTTATAAATATAACTTTaa                               >  1:472532/1‑141 (MQ=255)
                                                                                                               ttGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTaa                               >  1:274944/1‑141 (MQ=255)
                                                                                                                  cAGCTGAAAATGTTGAGAAGGTAGGTAAATAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTa                            >  1:482424/1‑141 (MQ=255)
                                                                                                                   aGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTAt                           <  2:169894/141‑1 (MQ=255)
                                                                                                                   aGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAAATTTAATTAt                           >  1:534325/1‑141 (MQ=255)
                                                                                                                   aGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTAGCCTTACCTGGTGATGGAATCGGTCCAGAAATTTTGCACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTAt                           >  2:32399/1‑141 (MQ=255)
                                                                                                                    gCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATc                          <  2:664086/141‑1 (MQ=255)
                                                                                                                        aaaaTGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCaaac                      >  2:760925/1‑140 (MQ=255)
                                                                                                                            tGTTGAGAAGGTAAGCAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGATCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATCAATATAACTTTAATCATCAAATacag                  >  2:398505/1‑141 (MQ=255)
                                                                                                                              ttGAGAAGGTAGGTGATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGca                <  1:92813/141‑1 (MQ=255)
                                                                                                                              ttGAGAAGGTAGGTGATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGca                <  1:92486/141‑1 (MQ=255)
                                                                                                                               tGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGcac               >  1:710269/1‑141 (MQ=255)
                                                                                                                                  gaAGGTAGGTAATTAATTATGACTTATAACAGTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGcaccac            <  1:269435/141‑1 (MQ=255)
                                                                                                                                   aaGGTAGGTAATTACTTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACg           <  2:611537/141‑1 (MQ=255)
                                                                                                                                    aggtaggtAATTAATTATGACTTATAACATTGTTGCCCTCCCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGa          <  1:493303/141‑1 (MQ=255)
                                                                                                                                       taggtaATTTATTATGTCTGATATCATTTTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAAtt       <  2:315747/141‑1 (MQ=255)
                                                                                                                                       taggtaATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAAtt       <  1:248252/141‑1 (MQ=255)
                                                                                                                                            aattaattATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTtggtg  <  1:250536/141‑1 (MQ=255)
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CAGAAGTACATGTAAATTTATTGATTGAAGGTAAGACTGTCAATGGCTTTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTTGAGAAGGTAGGTAATTAATTATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTG  >  CP000730/2171804‑2172084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: