Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 195,286 T→A 100% intergenic (‑97/‑82) USA300HOU_0183 ← / → USA300HOU_0184 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730195,2860TA100.0% 197.4 / ‑6.5 60intergenic (‑97/‑82)USA300HOU_0183/USA300HOU_0184hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base A (21/37);  minor base C (1/0);  total (23/37)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTCTAGCTAAACTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTTAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGATTTTGCATCAACGAAAGA  >  CP000730/195153‑195420
                                                                                                                                     |                                                                                                                                      
ttCTAGCTACACTTGCTACGACAGATGCTTTATCCATAATGATAGCCCTCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTaaaaaaaa                                                                                                                                 <  1:580220/141‑1 (MQ=255)
  cTAGCTAAACTTGCTACGACCGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAata                                                                                                                               >  1:479285/1‑141 (MQ=255)
      cTAAACTTTCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATTTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCtt                                                                                                                           <  2:305933/141‑1 (MQ=255)
         aaCTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAATATCTTAatt                                                                                                                       >  2:169866/1‑140 (MQ=255)
           cTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAatat                                                                                                                      >  1:444728/1‑141 (MQ=255)
            ttGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAACTATTAAAAAAAACATCTTAATATc                                                                                                                     <  1:483796/141‑1 (MQ=255)
            ttGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATc                                                                                                                     <  1:411838/141‑1 (MQ=255)
              gCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAATTATTATAAAAAATATCTTAATATcat                                                                                                                   >  1:623743/1‑139 (MQ=255)
                 aCGACAGATTTTTTATCCATAATGATAGCCCCCTATATACATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATTTAAATATTAAAAAAAATATCTTAATATCCTTGt                                                                                                                <  2:272843/141‑1 (MQ=255)
                         ttCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGAt                                                                                                        >  2:669389/1‑141 (MQ=255)
                          tCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATa                                                                                                       <  1:238109/141‑1 (MQ=255)
                          tCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATa                                                                                                       >  2:6616/1‑141 (MQ=255)
                          tCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATAACTTAATATCCTTGTAATCAGATa                                                                                                       >  2:386147/1‑141 (MQ=255)
                           cTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATaa                                                                                                      >  1:652219/1‑141 (MQ=255)
                               aTCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAAt                                                                                                  >  2:84659/1‑141 (MQ=255)
                                          tAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAatat                                                                                       >  1:531197/1‑141 (MQ=255)
                                          aaGCCCCCTATATATATGTTCATTGACATATACCCTAACATGATTTTTATACTATTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATGTCCTTGTAATCCGATAAGAATTATAGTAatat                                                                                       <  2:154909/140‑1 (MQ=255)
                                             cccccTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATAtttt                                                                                    >  1:128175/1‑141 (MQ=255)
                                              ctattatacataTGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATAttttt                                                                                   <  2:448491/137‑1 (MQ=255)
                                              cgccgatactgcaatttattgaCTACCACCATCACAAGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTCATGTAAATATTAAAAAAAATATCTTAATATTCTTGTAATCCGATAAGAATTATAGTAATAttttt                                                                                   <  1:545785/127‑1 (MQ=255)
                                              cctcTATAGCTATGTTTATTTACTTATACCCTTACATTATTTGTATGCTATTTGAAAATATAGTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATAGCCTTGTAATCCGATAAGAATTATAGTAATAttttt                                                                                   <  2:489089/138‑1 (MQ=255)
                                              ccccTATATAGAGGTTTAATTACTTAGACCCTAACAAGATTTTTATACGCTTGGAAAATATATTTTACAGAATATTATCAAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATAttttt                                                                                   <  2:142005/141‑1 (MQ=255)
                                                ccTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATAtttttta                                                                                 >  2:671977/1‑140 (MQ=255)
                                                             ttatttaCTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTtata                                                                    <  1:186507/141‑1 (MQ=255)
                                                               ttttaCTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATagg                                                                  <  2:727576/140‑1 (MQ=255)
                                                               ttttaCTTATACCCTAACATGATTTTTATACTCATATGCGAGATATTTCAGAGCATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAACCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATagg                                                                  <  1:514379/140‑1 (MQ=255)
                                                                tttaCTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATagga                                                                 <  1:51905/141‑1 (MQ=255)
                                                                   aatgacaCCCTAACATGATCTTTATAATCTTGGAAAATATATTTTACAGAATTTTATAAAAAAAAAAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGt                                                              <  2:138955/135‑1 (MQ=255)
                                                                   aCTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCCACCATTGTTATAGGAGGt                                                              >  1:337908/1‑141 (MQ=255)
                                                                    cTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTc                                                             <  1:760283/141‑1 (MQ=255)
                                                                    cTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTc                                                             <  1:27012/141‑1 (MQ=255)
                                                                     ttATACCCTAATATGATTTTTATTCTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGAAAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCt                                                            <  1:444417/141‑1 (MQ=255)
                                                                     ttATAACCTAACATGATTTTTATAATCTTTGAAAATATATTTTACAGAATTTTATAAAAAAATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCt                                                            <  2:719829/141‑1 (MQ=255)
                                                                        taCCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCttat                                                         <  1:621561/141‑1 (MQ=255)
                                                                          cccTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCttatta                                                       >  1:763280/1‑141 (MQ=255)
                                                                             ttacatttttgttATACTTTTTGAAAATATATTTTACAGAATTTTATCTAAATAATAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATGTTTTCAACCATTGTTATAGGAGGTCTGATTAATg                                                    <  2:276394/130‑1 (MQ=255)
                                                                             tAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAATTTTAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATg                                                    <  1:552598/141‑1 (MQ=255)
                                                                             tAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAATTTGTATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATg                                                    <  1:552500/141‑1 (MQ=255)
                                                                              aaCATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGa                                                   <  2:583386/141‑1 (MQ=255)
                                                                                cATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGGCTTATTAATAACa                                                 >  2:77883/1‑141 (MQ=255)
                                                                                   gATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACatta                                              <  1:570319/141‑1 (MQ=255)
                                                                                    ttttttATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACattat                                             <  1:264851/140‑1 (MQ=255)
                                                                                    aTTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTCAAAAAATGTGCTTAATATCATTGTAATCCGATAAGAATTATATTAATATTTTTTCAACCATTGTTATATGAGGTCTTATTAATGACattat                                             >  1:329342/1‑141 (MQ=255)
                                                                                     tttttATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGAAattatt                                            >  2:266497/1‑141 (MQ=255)
                                                                                      ttttATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTAttt                                           >  2:656085/1‑141 (MQ=255)
                                                                                      ttttATAATCTTTTAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTAttt                                           <  2:228578/141‑1 (MQ=255)
                                                                                         tataCTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTtta                                        <  1:6616/141‑1 (MQ=255)
                                                                                           taCTCTTTTAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTttatt                                      <  1:23418/141‑1 (MQ=255)
                                                                                            aatctTTGAAAATATATTTTACAGAATTTTATCTAAATATAAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTttatta                                     <  2:23264/139‑1 (MQ=255)
                                                                                             atccttGAAAATATATTTTACAGAATTTTATCAAAAAATAAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAg                                    <  2:534366/137‑1 (MQ=255)
                                                                                                      aataaataTTACAAAATTTTATCTAAATATTAAAAAATATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACa                           <  2:143639/141‑1 (MQ=255)
                                                                                                       atatatTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACaa                          >  2:65176/1‑141 (MQ=255)
                                                                                                        tatatTTTACAGAATTTTAACTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAAt                         <  2:444728/141‑1 (MQ=255)
                                                                                                              ttACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTTTTTTTATTAGAAGCTAACAATCTTGAt                   >  2:429959/1‑141 (MQ=255)
                                                                                                                atatttttttAGATAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGAttt                 <  2:153833/135‑1 (MQ=255)
                                                                                                                  aGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGATTTTg               <  1:311523/141‑1 (MQ=255)
                                                                                                                   gAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGATTTTGc              <  1:617295/141‑1 (MQ=255)
                                                                                                                         tATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCTACTTTTTTTATAGGAGGTCTTATTAATGATATTATTTTTATTAGAAGCTAATAATCTTGATTTTGCTTctat        >  2:267289/1‑138 (MQ=255)
                                                                                                                         tATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGATTTTGCATCAAc        >  1:405758/1‑141 (MQ=255)
                                                                                                                           tCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGATTTTGCATCAACGa      <  2:763280/141‑1 (MQ=255)
                                                                                                                               aaTATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGATTTTGCATCAACGAaaga  >  1:280419/1‑141 (MQ=255)
                                                                                                                                     |                                                                                                                                      
TTCTAGCTAAACTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTTAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGATTTTGCATCAACGAAAGA  >  CP000730/195153‑195420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: