| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 217,384 | A→G | 7.1% | M344T (ATG→ACG) | brnQ ← | possible LIVCS family branched chain amino acid:cation symporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 217,384 | 0 | A | G | 7.1% | 187.1 / 2.7 | 66 | M344T (ATG→ACG) | brnQ | possible LIVCS family branched chain amino acid:cation symporter |
| Reads supporting (aligned to +/- strand): ref base A (36/25); new base G (3/2); total (39/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCAGCAATCATTGTAAAGAAAATGGTACATTGATAAATAAGTTTTGAATGATGGAATTTGCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAGGGAAAAGGTGTTCGAATGTTTCTGAAAATGCCGTAATCAAACCGATGGCTGTTT > CP000730/217251‑217522 | tGCAGCAATCATTGTAAAGAAAATGGTACATTGATAAATAAGTTTTGAATGATGGAATTTGCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATaataat > 1:435308/1‑141 (MQ=255) cagcaATCATTGGAAAGAAAATGGTACATTGATAAATAAGTTTTGAATGATGGAATTTGCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCt < 1:113551/141‑1 (MQ=255) gcaATCATTGCAAAGAAAATGGTAGATTGATAAATAAGTTTTGAATGATGGAATTTGCTACTAAATTCGTTTATTACAATCAAGGCAATTGCTAATGAATATAAGAACATTAACACTGGGACTGATTACGTAAGAATCTTa > 1:556281/1‑141 (MQ=255) gcaATCATTAAAAAGAAAATGGTAAATTGATAAATAAGTTTTGAATGTTCTCTTGTGCTTCTAAATAATGTTAGTACAATCAAGGCAATTACTAAGGTATATAAGAACATTAACACTGGGACTGAGTACGTAATAATCTTa > 1:556284/1‑141 (MQ=255) tGTAAAGAAAATGGTACATTGATAAATAAGTTTTGAATGATGGAATTTGCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAAcc > 1:141244/1‑141 (MQ=255) gTAAAGAAAATGGTACATTGATAAATAAGTTTTGAATGATGGAATTTGCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCa > 2:528809/1‑141 (MQ=255) aaGAAAATGATATATGGAAAAATAACTTTTGATGGATGGAATTTGCTACTAAAAAATGTTAGTACAACCAAGGCAGTTGCTACTGGATATAAGAACATTAACACTGGGGCTGAGTACATAATAGTTTTAGTTAAACCGACa < 2:574262/141‑1 (MQ=255) aaaaAGGTACATTGATAAATAAGTTTTGAATGATGGAATTTGCTAATAAATAATGTTGGTACAATCAAGGTAATTTCTAATGGATAAAAGTACAATAACACTATAAACAAGTACATAATATTCTTAGTTAAACCAACATTc > 2:116675/1‑141 (MQ=255) aagggTACATTGATAAATAAGTTTTGAATGATGGAATTTGCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTAATGGACATAAGAACATCAACACTGGGACTGAGTACATAATCATCTTAGTTAAACCAACATTcgc < 2:725202/138‑1 (MQ=255) aTGGTACATTGATAAATAAGTTTTGAATGATGGAATTTGCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTcgca > 1:71612/1‑140 (MQ=255) gTACATTGATAAATAAGTTTTGAATGATGGAATTTGCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAAt < 2:507278/141‑1 (MQ=255) gTACATTGATAAATAAGTTTTGAATGATGGAATTTGCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAAt > 2:313665/1‑141 (MQ=255) tACATTGATAAATAAGTTTTGAATGATGGAATTTGCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAAAACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATa > 1:567065/1‑141 (MQ=255) aCATGGATAAAGGAGTTTGGAATGAGGGAAGTTGCTACTAAATAATGTGAGTACAAGCGAGGCGGGGGCTGATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATaa < 1:436271/141‑1 (MQ=255) taaataaGTTTTGAATGATGGAATTTGCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGaa > 1:327502/1‑141 (MQ=255) aataaGTTTTGAATGATGGAATTTGCTACTAAATAATTTTAGTGCAATCAAGGCGATTGCTAATGGATATAAGAAATTTAAAACTGGGACTGAGTACGTAAGAATATTATGTAAACAAACCCTCACTAAAAAGAGCGaaaa > 2:97474/1‑140 (MQ=255) aataaGTTTTGAATGATGGAATTTGCTACTAAATAATATTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGTGAATAAGAAGGaaaa > 2:513228/1‑140 (MQ=255) ataaGTTTTGAATGATGGAATTTGCTACTAAATAATGTTAGTACAATCAATGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATa > 2:558281/1‑141 (MQ=255) taaGTTTTGAATGATGGAATTTGCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACAGGCCCAGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATaa > 1:747758/1‑141 (MQ=255) aGTTTTGAATGATGGAATTTGCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGCACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAGTAAGACCGAAATAAAg > 1:6830/1‑141 (MQ=255) gTTTTGAATGATGGAATTTGCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGc > 2:137733/1‑141 (MQ=255) tttGAATGATGGAATTTGCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCtt < 1:484819/141‑1 (MQ=255) tttGAATGATGGAATTTGCTACGAAATAATGTTAGTACAAGCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCtt < 2:570023/141‑1 (MQ=255) tGAATGATGGAATTTGCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACCTTCCCGAATAAGAATAGAATAAAGCTTAc > 2:23269/1‑141 (MQ=255) aaTGATGGAATTTGCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACaa > 1:47304/1‑141 (MQ=255) tgatgGAATTTGCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTGATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCGTAGTTAAACCAACATTCGCGAACAAAAACGAAATAAAGCTTAAAACt > 2:503283/1‑141 (MQ=255) atgGAATTTGCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGt > 1:470993/1‑141 (MQ=255) ggAATTTGCTACTAAATAATGTTAGTACAATTAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCGTGCAACTGTTg < 2:165808/141‑1 (MQ=255) aTTTGCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCaa > 2:583203/1‑141 (MQ=255) aTTTGCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACAGAAAAATCTTAGTTAAACCAACATTCGCGAATAAGCACGAAATAAAGCTTACAACAGTTGCaa > 2:81006/1‑141 (MQ=255) ttGCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGACATAAAGCTTACAACTGTTGCAATc < 1:554147/141‑1 (MQ=255) gCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGc > 2:744730/1‑141 (MQ=255) ctaAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAgg < 1:313665/141‑1 (MQ=255) ctaAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAgg > 2:747711/1‑141 (MQ=255) aataatGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACGTAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTATAACTGTTGCAATCGCTAGGTaa < 2:556281/141‑1 (MQ=255) aataatGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACGTAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTaa < 2:556284/141‑1 (MQ=255) ataatGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAAt < 1:528809/141‑1 (MQ=255) atGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCa > 2:645872/1‑141 (MQ=255) tGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACGGTTGCAATCGCTAGGTAATTCAt > 1:289208/1‑141 (MQ=255) ttAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCAttt < 2:370632/141‑1 (MQ=255) ttAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCAttt < 1:707478/141‑1 (MQ=255) ttACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACGGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAg < 1:18358/140‑1 (MQ=255) tACAATCAAGGCAATTGCTAATGGATATAAGAACAGTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAgg < 2:72030/141‑1 (MQ=255) aaTCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAGGGaa > 2:613191/1‑141 (MQ=255) aTCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAGGGaaa > 1:240865/1‑141 (MQ=255) aGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAGGGAAAAGgt > 1:272355/1‑141 (MQ=255) gtaaTTGCTAATTGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAGGGAAAAGgtgt < 2:197709/139‑1 (MQ=255) gCAATTGCGAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATACGGACGACCAAAAGCCAACAACTGTTGTAATCGCTAGGTAATTCATTTTAGGGAAAAGgtgt < 1:677619/141‑1 (MQ=255) cAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAGGGAAAAGGTGtt < 2:141244/141‑1 (MQ=255) ttGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAGGGAAAAGGTGTTGGa > 1:742704/1‑141 (MQ=255) ttGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAGGGAAAAGGTGTTCGa > 1:399290/1‑141 (MQ=255) aTGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAGGGAAAAGGTGTTCGAATGttt < 1:746574/141‑1 (MQ=255) aTGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAGGGAAAAGGTGTTCGAATGgtt > 1:708024/1‑141 (MQ=255) ggATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAGGGAAAAGGTGTTCGAATGTTTCt < 2:300756/141‑1 (MQ=255) ggATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAGGGAAAAGGTGTTCGAATGTTTCt < 1:358763/141‑1 (MQ=255) ataAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAGGGAAAAGGTGTTCGAATGTTTCTGaaa > 1:108094/1‑141 (MQ=255) taAGAACATTAACACTGGGAATGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAGGGAAAAGGTGTTCGAATGTTTCTTaaaa > 1:460193/1‑141 (MQ=255) aaGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAAGCGCTAGGTAATTCATTTTAGGGAAAAGGTGTTCGAATGTTTCTGAGAAt > 2:221356/1‑141 (MQ=255) aGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAGGGAAAAGGTGTTCGAATGTTTCTGAAAATg > 2:134603/1‑141 (MQ=255) ttAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAGGGAAAAGGTGTTCGAATGTTTCTGAAAATGCCGTaa < 2:297959/141‑1 (MQ=255) ttAACACTGGGACTGAGTACATAATAAACTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAATCTTACAACTGTTGCAATCGCTTGGTAATTCATTTTATGGAAAAGGTGTTCTAATGTTTCTGAAAATGCCGTaa < 2:47304/141‑1 (MQ=255) tAACACTGGGACTGAGAACACAATAATCTTAGGTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAGGGAAAAGGTGTTCGAATGTTTCTGAAAATGCCGTAAt < 1:15190/141‑1 (MQ=255) aaCACCGGGACTGAGTACATAATAATCTTAGTTACACCACCATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAGGGAAAAGGTGTTCGAATGTTTCTGAAAATGCCGTaaac > 2:364637/1‑139 (MQ=255) acacTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAGGGAAAAGGTGTTCGAATGTTTCTGAAAATGCCGTAATCa < 1:137733/141‑1 (MQ=255) tGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAGGGAAAAGGTGTTCGAATGTTTATGCCAATGCCGTAATCAAACCGATGGc > 1:14948/1‑141 (MQ=255) aCATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAGGGAAAAGGTGTTCGAATGTTTCTGAAAATGCCGTAATCAAACCGGTGGCTGttt > 2:703641/1‑141 (MQ=255) | TGCAGCAATCATTGTAAAGAAAATGGTACATTGATAAATAAGTTTTGAATGATGGAATTTGCTACTAAATAATGTTAGTACAATCAAGGCAATTGCTAATGGATATAAGAACATTAACACTGGGACTGAGTACATAATAATCTTAGTTAAACCAACATTCGCGAATAAGAACGAAATAAAGCTTACAACTGTTGCAATCGCTAGGTAATTCATTTTAGGGAAAAGGTGTTCGAATGTTTCTGAAAATGCCGTAATCAAACCGATGGCTGTTT > CP000730/217251‑217522 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |