Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,028,269	G→C	P126A (CCA→GCA)	yvqF ←	hypothetical membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,028,269	0	G	C	100.0%	150.0 / NA	40	P126A (CCA→GCA)	yvqF	hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (23/17);  total (23/17)

ACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACG  >  CP000730/2028144‑2028399                                                                                                                              |                                                                                                                                   aCCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCAcc                                                                                                                     <  1:163576/141‑1 (MQ=255)          gtgtCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATTTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACtt                                                                                                            >  2:97395/1‑141 (MQ=255)             tCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTg                                                                                                         >  1:256973/1‑141 (MQ=255)              cTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGt                                                                                                        <  1:110334/141‑1 (MQ=255)              cTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGt                                                                                                        >  2:264182/1‑141 (MQ=255)                  caacaaTGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTtaa                                                                                                    <  1:156415/141‑1 (MQ=255)                        tGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAg                                                                                              >  1:154151/1‑141 (MQ=255)                         ggTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGa                                                                                             >  1:233170/1‑141 (MQ=255)                          gTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGAt                                                                                            >  2:219901/1‑141 (MQ=255)                              tattTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATggcg                                                                                        >  2:41422/1‑139 (MQ=255)                              tattTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTg                                                                                        <  1:36235/141‑1 (MQ=255)                                      ttAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATa                                                                                >  1:250979/1‑141 (MQ=255)                                             ttGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCAt                                                                         >  2:360821/1‑141 (MQ=255)                                               gCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTCCGTTGCTAACAAACACGCTATGCCATTGTAAAAGATTTTGAAGTCATAACCTCAGcc                                                                       >  1:76064/1‑141 (MQ=255)                                               gCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATcc                                                                       >  2:184215/1‑141 (MQ=255)                                               gCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATcc                                                                       >  1:376947/1‑141 (MQ=255)                                                cAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCg                                                                      >  2:336063/1‑141 (MQ=255)                                                 aGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCGATGTTGAATTTGTACGTCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGt                                                                     <  2:368047/141‑1 (MQ=255)                                                     tttGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCa                                                                 >  2:163413/1‑141 (MQ=255)                                                      ttGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCaa                                                                >  2:193425/1‑141 (MQ=255)                                                        gTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAact                                                              >  1:173187/1‑141 (MQ=255)                                                        gTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAact                                                              <  2:74366/141‑1 (MQ=255)                                                         ttAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAacta                                                             >  1:270558/1‑141 (MQ=255)                                                         ttAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAacta                                                             >  2:246212/1‑141 (MQ=255)                                                         ttAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACACAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAacta                                                             <  2:207523/141‑1 (MQ=255)                                                               cAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACttttt                                                       >  1:326775/1‑141 (MQ=255)                                                                   atGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTgg                                                   <  2:233170/141‑1 (MQ=255)                                                                      tAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGttt                                                <  2:196606/141‑1 (MQ=255)                                                                      tAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGttt                                                <  2:318417/141‑1 (MQ=255)                                                                             ccAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTtg                                         >  1:19180/1‑141 (MQ=255)                                                                                 ttCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTa                                     <  2:6397/141‑1 (MQ=255)                                                                                 ttCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTa                                     <  1:56988/141‑1 (MQ=255)                                                                                    cATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTa                                  <  1:47716/141‑1 (MQ=255)                                                                                    cATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTa                                  <  1:47608/141‑1 (MQ=255)                                                                                           aaTTTGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACg                           <  2:270558/141‑1 (MQ=255)                                                                                               tGTACATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGcaca                       <  1:219901/141‑1 (MQ=255)                                                                                                  aCATCTTCCCACTTATATACATAAACTGCTGTACGTTGCTCACCAAACCACTTTTTTTTATTTTAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATa                    >  2:353212/1‑141 (MQ=255)                                                                                                                  ataCATAAACTGCTGTACGATGCTCACCAAACCACTTTTGCTTCATAAAGGATGGAGAAGTCATAACCTCATCCGTCGAAACTACTTTTTTTGTTTTAAACTTGAGGATTAAATAAAGTACAGAAAGtaataagatccgta    >  2:337734/1‑133 (MQ=255)                                                                                                                   taCATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGAc   >  2:168932/1‑141 (MQ=255)                                                                                                                    aCATAAACTGCTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACg  <  1:360821/141‑1 (MQ=255)                                                                                                                              |                                                                                                                                   ACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATAAACTGGTGTACGTTGCTCACCAAACCACTTTTGTTTAATAAAAGATGGTGAAGTCATAACCTCATCCGTCGCAACTACTTTTTTTGGTTTAAACTTGTGTATTAAATAACGCACAATAAGTAATAATAAAAAGACG  >  CP000730/2028144‑2028399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑