Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,371,220	C→T	intergenic (‑4337/+1221)	rpmC ← / ← pbuG	ribosomal protein L29/NCS2 family nucleobase:cation symporter‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,371,220	0	C	T	100.0%	139.5 / NA	40	intergenic (‑4337/+1221)	rpmC/pbuG	ribosomal protein L29/NCS2 family nucleobase:cation symporter‑2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/13);  total (27/13)

TACTACTGTTACAGGGATTAATTCACCGTTTTCTCCGAATACTTGTGTCATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACACCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCACGTGAATCTTTATACTTATGCACGGCACGGATGAT  >  CP000730/2371084‑2371353                                                                                                                                         |                                                                                                                                      tactacTGTTACAGGGATTAATTCACCGTTTTCTCCGAATACTTGTGTCATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGa                                                                                                                                   >  1:13405/1‑141 (MQ=255)   ctactGTTACAGGGATTAATTCACCGTTTTCTCCGAATACTTGTGTCATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATg                                                                                                                                 <  1:208166/141‑1 (MQ=255)    tactGTTACAGGGATTAATTCACCGATTACTCCGAAAACTTTTGTCATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATgg                                                                                                                                >  2:214720/1‑141 (MQ=255)       tGTTACAGGGATTAATTCACCGTTTTCTCCGAATACTTGTGTCATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTaa                                                                                                                             >  1:288702/1‑141 (MQ=255)       tGTTACAGGGATTAATTCACCGTTTTCTCCGAATACTTGTGTCATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTaa                                                                                                                             >  1:288726/1‑141 (MQ=255)                      ttCACCGTTTTCTCCGAATACTTGTGTCATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTaa                                                                                                              >  2:88415/1‑141 (MQ=255)                      ttCACCGTTTTCTCCGAATACTTGTGTCATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTaa                                                                                                              >  2:206299/1‑141 (MQ=255)                                    cGAATACTTGTGTCATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGttt                                                                                                >  1:171414/1‑141 (MQ=255)                                     gAATACTTGTGTCATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGtttt                                                                                               <  2:141413/141‑1 (MQ=255)                                       aTACTTGTGTCATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTg                                                                                             >  1:6836/1‑141 (MQ=255)                                           ttGTGTCATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGgtgt                                                                                         <  2:325532/141‑1 (MQ=255)                                           ttGTGTCATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGgtgt                                                                                         <  2:157282/141‑1 (MQ=255)                                              tgtCATCCCAATTTTTCTTTTTTTTTTTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCATCTGTTTTTGGTGTTgg                                                                                      >  2:55453/1‑141 (MQ=255)                                              tgtCATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCCCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACCGTTTCTGGTGTTgg                                                                                      >  2:115040/1‑141 (MQ=255)                                                 cATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGtt                                                                                   <  1:131692/141‑1 (MQ=255)                                                 cATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGtt                                                                                   <  1:236678/141‑1 (MQ=255)                                                    cccAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTAc                                                                                >  1:231357/1‑141 (MQ=255)                                                      cAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACaa                                                                              >  2:376774/1‑141 (MQ=255)                                                             tcttcCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGAt                                                                       <  2:13405/141‑1 (MQ=255)                                                              ttttttttttttCCTTTGGTCATCGAAACTCCACCTCCTAAACTTGTCTATTGTAATTTGATTTCGATGTCTACATCAGATGGTATTTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGAtt                                                                      >  2:335319/11‑141 (MQ=255)                                                                tcCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTaa                                                                    >  2:258201/1‑141 (MQ=255)                                                                            tttGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTgtgtgt                                                        >  2:7511/1‑141 (MQ=255)                                                                            tttGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTgtgtgt                                                        <  1:206377/141‑1 (MQ=255)                                                                              tGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTAc                                                      >  2:261951/1‑141 (MQ=255)                                                                                               cctcctAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGCATTGttaa                                     >  1:99553/1‑139 (MQ=255)                                                                                               cctcctAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCa                                     <  1:296422/141‑1 (MQ=255)                                                                                               cctcctAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCa                                     >  1:112962/1‑141 (MQ=255)                                                                                               cctcctAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCa                                     >  2:37747/1‑141 (MQ=255)                                                                                                ctcctAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCAc                                    <  2:35479/141‑1 (MQ=255)                                                                                                 tcctAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCACg                                   <  2:118954/141‑1 (MQ=255)                                                                                                    tAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTCAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCACGTGa                                >  1:374857/1‑141 (MQ=255)                                                                                                        aTTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCACGTGAATCt                            >  1:162762/1‑141 (MQ=255)                                                                                                                        tGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCACGTGAATCTTTATACTTATgcacgg            >  2:363290/1‑141 (MQ=255)                                                                                                                          aTTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCACGTGAATCTTTATACTTATgcacggca          <  1:88415/141‑1 (MQ=255)                                                                                                                           tttCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGCTTAAGCGTTTGTGTGTACGTTGTTCGAATTGTTCACGTGAATCTTTATACTTATgcacggcac         >  1:106306/1‑141 (MQ=255)                                                                                                                           tttCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCACGTGAATCTTTATACTTATgcacggcac         >  1:385104/1‑141 (MQ=255)                                                                                                                             tCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCACGTGAATCTTTATACTTATGcacggcacgg       <  1:376774/141‑1 (MQ=255)                                                                                                                                aTGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCACGTGAATCTTTATACTTATGCACGGCACGgatg    >  1:213990/1‑141 (MQ=255)                                                                                                                                 tGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCACGTGAATCTTTATACTTATGCACGGCACGgatgt   >  1:2401/1‑140 (MQ=255)                                                                                                                                  gTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGTTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCACGTGAATCTTTATACTTATGCACGGCACGgatgat  >  2:31448/1‑141 (MQ=255)                                                                                                                                         |                                                                                                                                      TACTACTGTTACAGGGATTAATTCACCGTTTTCTCCGAATACTTGTGTCATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACACCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCACGTGAATCTTTATACTTATGCACGGCACGGATGAT  >  CP000730/2371084‑2371353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑