Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,816,469	G→C	Y620* (TAC→TAG)	sasA ←	serine‑threonine rich protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,816,469	0	G	C	100.0%	171.2 / NA	45	Y620* (TAC→TAG)	sasA	serine‑threonine rich protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (23/22);  total (23/22)

GTTGGTGCTGTCGTATCCACAACATTTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCTACAGTTACAGTTGGTGCT  >  CP000730/2816332‑2816604                                                                                                                                          |                                                                                                                                        gTTGGTGCTGTCGTATCCACAACATTTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTaa                                                                                                                                      >  2:226887/1‑141 (MQ=255)  ttGGTGCTGTCGTATCCACAACATTTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAAc                                                                                                                                     >  2:117247/1‑141 (MQ=255)    ggTGCTGTCGTATCCACAACATTTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACtt                                                                                                                                   <  1:269069/141‑1 (MQ=255)             gTATCCACAACATTTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCtt                                                                                                                          >  2:238742/1‑141 (MQ=255)             gTATCCACAACATTTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCtt                                                                                                                          >  2:67545/1‑141 (MQ=255)                 ccACAACATTTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTa                                                                                                                      <  2:249810/141‑1 (MQ=255)                    caacaTTTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATc                                                                                                                   <  2:49178/141‑1 (MQ=255)                         tttATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTa                                                                                                              <  1:313950/141‑1 (MQ=255)                           tATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCCCTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATGCGCTTGGTAATCCTGTAAc                                                                                                            >  2:157875/1‑141 (MQ=255)                            aTTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACt                                                                                                           <  2:159687/141‑1 (MQ=255)                                        gtcgtcGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTcaca                                                                                               >  1:294591/1‑141 (MQ=255)                                          cgtcgATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGt                                                                                             <  1:277009/141‑1 (MQ=255)                                          cgtcgATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGt                                                                                             >  1:281216/1‑141 (MQ=255)                                          cgtcgATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGt                                                                                             <  2:108777/141‑1 (MQ=255)                                          cgtcgATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGt                                                                                             <  1:379338/141‑1 (MQ=255)                                          cgtcgATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGt                                                                                             <  2:225115/141‑1 (MQ=255)                                           gtcgATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTc                                                                                            <  1:164827/141‑1 (MQ=255)                                             cgATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTccc                                                                                          <  1:108701/141‑1 (MQ=255)                                              gATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCa                                                                                         >  1:238819/1‑141 (MQ=255)                                                     tATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTTTTTTCTCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCAt                                                                                  >  2:99566/1‑141 (MQ=255)                                                     tATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCAt                                                                                  <  2:111580/141‑1 (MQ=255)                                                            ttGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGt                                                                           >  1:286189/1‑141 (MQ=255)                                                                  aGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGt                                                                     <  2:184142/141‑1 (MQ=255)                                                                        cacaACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTcaatac                                                               <  1:191823/141‑1 (MQ=255)                                                                        cacaACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTcaatac                                                               <  1:220851/141‑1 (MQ=255)                                                                            aCTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTcaatacaata                                                           >  1:2973/1‑141 (MQ=255)                                                                            aCTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTcaatacaata                                                           >  1:389451/1‑141 (MQ=255)                                                                                   cTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCa                                                    <  1:117247/141‑1 (MQ=255)                                                                                    tGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCAt                                                   >  1:140648/1‑141 (MQ=255)                                                                                    tGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCAt                                                   >  1:140649/1‑141 (MQ=255)                                                                                       tgattgaCCACTTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTCCCTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGt                                                <  1:293385/141‑1 (MQ=255)                                                                                               cAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGGAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTCGTAGTCAATGCAATAGGATCCATTGTTTTACCCa                                        >  2:17967/1‑141 (MQ=255)                                                                                                          ggTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGtt                             >  1:12210/1‑141 (MQ=255)                                                                                                          ggTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGtt                             <  1:186894/141‑1 (MQ=255)                                                                                                            tgtCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTg                           <  2:294591/141‑1 (MQ=255)                                                                                                                 cAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGc                      <  2:281216/141‑1 (MQ=255)                                                                                                                    tGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCta                   >  2:345812/1‑141 (MQ=255)                                                                                                                      aTTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCtaca                 >  2:271055/1‑141 (MQ=255)                                                                                                                           aTTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCtacagttac            >  2:209452/1‑141 (MQ=255)                                                                                                                           aTTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCtacagttac            >  2:129575/1‑141 (MQ=255)                                                                                                                               gTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCTacagttacagtt        <  1:311585/141‑1 (MQ=255)                                                                                                                                ttGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTCACTGTATTTTTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTGATTGTTTTACCCACTTCTATCGTTTGATTGCCTATAGTNTCAGATg       >  2:293267/1‑141 (MQ=255)                                                                                                                                   cACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCTACAGTTACAGTTGGTg    >  2:96354/1‑141 (MQ=255)                                                                                                                                     cTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCTACAGTTACAGTTGGTGCt  <  2:184328/141‑1 (MQ=255)                                                                                                                                     cTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCTACAGTTACAGTTGGTGCt  >  1:391443/1‑141 (MQ=255)                                                                                                                                          |                                                                                                                                        GTTGGTGCTGTCGTATCCACAACATTTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCTACAGTTACAGTTGGTGCT  >  CP000730/2816332‑2816604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑