Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 9,085 G→A 50.3% R685H (CGT→CAT)  gyrA → DNA topoisomerase (ATP‑hydrolyzing) subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007309,0850GA50.3% ‑3.8 / 129.7 95R685H (CGT→CAT) gyrADNA topoisomerase (ATP‑hydrolyzing) subunit A
Reads supporting (aligned to +/- strand):  ref base G (19/28);  new base A (19/28);  total (39/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01

GGTAAGATTGCGATTTCGTTCAGAGAAGATGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGA  >  CP000730/8946‑9223
                                                                                                                                           |                                                                                                                                          
ggTAAGATTGCGATTTCGTTCAGAGAAGATGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGt                                                                                                                                           <  1:453825/141‑1 (MQ=255)
   aaGATTGCGATTTCGTTCAGAGAAGATGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACa                                                                                                                                        <  1:347525/141‑1 (MQ=255)
   aaGATTGCGATTTCGTTCAGAGAAGATGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACa                                                                                                                                        <  1:72620/141‑1 (MQ=255)
          cGATTTCGTTCAGAGAAGATGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACgg                                                                                                                                 >  1:736447/1‑141 (MQ=255)
           gATTTCGTTCAGAGAATATGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACggg                                                                                                                                >  1:429072/1‑141 (MQ=255)
            aTTTCGTTCAGAGAAGATGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGgt                                                                                                                               <  2:342898/141‑1 (MQ=255)
             tttCGTTCAGAGAAGATGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGgtg                                                                                                                              <  2:777364/141‑1 (MQ=255)
             tttCGTTCAGAGAAGATGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGgtg                                                                                                                              <  1:658512/141‑1 (MQ=255)
             tttCGTGCAGAGAAGAGGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGgtg                                                                                                                              <  1:288706/141‑1 (MQ=255)
               tCGTTCAGAGAAGATGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGtgtg                                                                                                                            >  2:479644/1‑141 (MQ=255)
               tCGTTCAGAGAAGATGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATCCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGtgtg                                                                                                                            <  1:28530/141‑1 (MQ=255)
                cGTTCAGAGAAGAGGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCCTGATTGGTACAGCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGa                                                                                                                           <  1:866244/141‑1 (MQ=255)
                   tCAGAGAAGATGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGATGTGAAAg                                                                                                                        >  1:436871/1‑141 (MQ=255)
                    cAGAGAAGATGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGCAGCTGTCTTGCTTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAgg                                                                                                                       >  2:678269/1‑141 (MQ=255)
                       agaagaTGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAGGGTAt                                                                                                                    >  2:437644/1‑141 (MQ=255)
                       agaagaTGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTAt                                                                                                                    <  2:688617/141‑1 (MQ=255)
                       agaagaTGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTGAGGCCGTACAGCAACGGGTGTGAAAGGTAt                                                                                                                    <  1:87907/141‑1 (MQ=255)
                        gaagaTGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTAtt                                                                                                                   <  2:652655/141‑1 (MQ=255)
                          atttgatggGTTATAGGCATTTCGCTAAACAAGTGGCCAAGAAGATAGCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTac                                                                                                                 <  2:643512/138‑1 (MQ=255)
                              gatgaGTTAATTGCAGATCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACtt                                                                                                             >  1:482356/1‑141 (MQ=255)
                               atgaGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTc                                                                                                            >  1:652780/1‑141 (MQ=255)
                                tgaGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCGCTACACCAACGGGTGTGAAAGGTATTACACTTCg                                                                                                           >  2:413634/1‑141 (MQ=255)
                                tgaGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCg                                                                                                           >  1:373487/1‑141 (MQ=255)
                                    ttAATTGCAGTTCGCTTAAGAAGTGGTCAAGAAGATATCTTGGTTGGTACATCACCTGCATCATTAATTCGATTCCCTGACTCAACATTACGTCGTTTATGCCGTACGGCAACGGGTGTGAAAGCTGTTAGAGTTCGCGag                                                                                                       >  2:334560/1‑140 (MQ=255)
                                       atgtcAGTTCGCTTAACAAGTGGTCAAGACGTTATCTTGGTGGGTACATAGCATGCTTTAGTAATCCGATGCACCGTTTCAACGTTACGTCCTTTAAACCATACAGCACCGGGTGTGAAAGGTATTGCACTTAGTGACGGt                                                                                                    <  2:379837/137‑1 (MQ=255)
                                        ttGCAGTTCGCTTAAAAACTGCTCAAGAAGATATCTTGATTGTTACATCACATGCATCATTAATTCGATTCCCTGAATAAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTg                                                                                                   <  1:329955/141‑1 (MQ=255)
                                           cAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACg                                                                                                <  1:220726/141‑1 (MQ=255)
                                           cAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACg                                                                                                >  1:714451/1‑141 (MQ=255)
                                           cAGTTCGCTTAACAAGCGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACg                                                                                                <  2:456922/141‑1 (MQ=255)
                                             gTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGaa                                                                                              >  2:755106/1‑141 (MQ=255)
                                                cGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGtg                                                                                           >  2:439611/1‑140 (MQ=255)
                                                cGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGGAAGGTAGTACACTTCGTGAAGGTGACGAAGtt                                                                                           >  1:718307/1‑141 (MQ=255)
                                                cGCTTAACAAGTGGTCAAGAAGATATCTTGATGGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGtt                                                                                           >  2:206463/1‑141 (MQ=255)
                                                 gCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTg                                                                                          <  2:482356/141‑1 (MQ=255)
                                                   ttAACAAGTGGTCAATAAGATATCTTGATTGGTACCTCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTCAGGCCGTACAGCAACGGGTGTGAAAGGTACTACACTTCGTGAAGGTGACGAAGTTGTa                                                                                        <  2:61166/141‑1 (MQ=255)
                                                   ttAACAAGTGGTCAAGTAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTCAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTCCGTGAATGTGACGAAGTTGTa                                                                                        <  2:593821/141‑1 (MQ=255)
                                                   ttAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTa                                                                                        <  2:389337/141‑1 (MQ=255)
                                                       cAAGTGGTCAAGAAGATATCTTGATTGGTACACCCCATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGc                                                                                    <  1:720585/141‑1 (MQ=255)
                                                          gTGGTCAAGAAGATCTCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTg                                                                                 <  1:539118/141‑1 (MQ=255)
                                                          gTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGCGACGAAGTTGTAGGGCTTg                                                                                 <  1:459619/141‑1 (MQ=255)
                                                              tCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGt                                                                             >  1:831067/1‑141 (MQ=255)
                                                               cAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGGTGTAGGGCTTGATGTa                                                                            >  1:744545/1‑141 (MQ=255)
                                                               cAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTa                                                                            >  2:19863/1‑141 (MQ=255)
                                                               cAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTa                                                                            <  1:471492/141‑1 (MQ=255)
                                                               cAAGAAGATATCTTGATTGGTACATCACATGCATCATTAAATCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTATAGGGCTTGATGTa                                                                            >  1:619057/1‑141 (MQ=255)
                                                               cAAGAAGATATCTTGATTGGGACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTa                                                                            >  1:523989/1‑141 (MQ=255)
                                                                      atatCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATg                                                                     >  2:156279/1‑141 (MQ=255)
                                                                        atCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTCCGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGGATTACACTTCGTGAGGGTGAGGAAGTTGTAGGGCTTGATGTTGCTcgggca                                                                   >  2:227733/1‑136 (MQ=255)
                                                                          cTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCaaa                                                                 <  1:834935/141‑1 (MQ=255)
                                                                           ttGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGAGGTAGCTCATGCAAAc                                                                >  2:658018/1‑141 (MQ=255)
                                                                           ttGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAAc                                                                <  1:768889/141‑1 (MQ=255)
                                                                           ttGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAAc                                                                <  2:549724/141‑1 (MQ=255)
                                                                              aTTGGTACATCACATGCATCATTAATTCGCTGCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGTAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAgt                                                             <  2:202690/141‑1 (MQ=255)
                                                                               ttGGTACATCCCATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAgtg                                                            <  1:765855/141‑1 (MQ=255)
                                                                               ttGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAgtg                                                            <  1:237819/141‑1 (MQ=255)
                                                                               ttGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAgtg                                                            <  2:510327/141‑1 (MQ=255)
                                                                                tGGTACGTCACGTGGATCATTAATTCGATTCCCTGGATCAACATTACGTCCTTTAGGCCATAGAGCAAGGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAGCAgtgt                                                           <  2:357218/141‑1 (MQ=255)
                                                                                  gTACCTCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTtg                                                         <  2:256378/141‑1 (MQ=255)
                                                                                   tACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTtga                                                        >  2:338937/1‑141 (MQ=255)
                                                                                   tACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGAGTGAAAGGTATTACATTTAGCAAATTGAATGGCGTTGTAGGGGCGGAGTTAGCTCATGCAAACAGTGTtga                                                        >  1:847715/1‑141 (MQ=255)
                                                                                    aCATCACATGCATCATTAGTTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTtgat                                                       <  2:148279/141‑1 (MQ=255)
                                                                                     cATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTtgatg                                                      <  2:221543/141‑1 (MQ=255)
                                                                                       tCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGaa                                                    <  1:369481/141‑1 (MQ=255)
                                                                                           aTGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTAt                                                <  2:429072/141‑1 (MQ=255)
                                                                                              catcatTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATtag                                             <  2:436871/141‑1 (MQ=255)
                                                                                              catcatTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATtag                                             <  1:479644/141‑1 (MQ=255)
                                                                                                tcatTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCCTGCAAACAGTGTTGATGAAGTATtagta                                           <  1:548697/141‑1 (MQ=255)
                                                                                                 catTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATtagtag                                          <  2:200227/141‑1 (MQ=255)
                                                                                                  atTAATTCGTCTCCCTGAATCAACATTACTTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTagtagt                                         <  2:171373/141‑1 (MQ=255)
                                                                                                  atTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGAATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTagtagt                                         >  1:79982/1‑141 (MQ=255)
                                                                                                   ttAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGtt                                        <  2:736447/141‑1 (MQ=255)
                                                                                                    tAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTa                                       <  1:353537/141‑1 (MQ=255)
                                                                                                    tAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCAAACAGCAACGAGTGTGAAAGATCATACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTa                                       <  1:680611/141‑1 (MQ=255)
                                                                                                       ttCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTg                                    <  1:707248/141‑1 (MQ=255)
                                                                                                           aTTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGaaaa                                <  1:466677/141‑1 (MQ=255)
                                                                                                           aTTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGaaaa                                <  2:728497/141‑1 (MQ=255)
                                                                                                            ttCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAGGTATTAGTAGTTCGTGaaaag                               >  2:533426/1‑140 (MQ=255)
                                                                                                                cTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCGTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGtt                           >  1:245596/1‑141 (MQ=255)
                                                                                                                 tcatacAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGGATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTa                          >  2:435708/6‑141 (MQ=255)
                                                                                                                  gAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTAt                         <  1:169582/141‑1 (MQ=255)
                                                                                                                    aTCAACATTACGTCCTTTAGGCCATACAGCCACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACCGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATgg                       <  2:635953/141‑1 (MQ=255)
                                                                                                                     tCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGt                      >  1:325256/1‑141 (MQ=255)
                                                                                                                      cAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTa                     <  1:846025/141‑1 (MQ=255)
                                                                                                                        aCATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTACTTACTGAAAATGGTTATGGTaaa                   <  2:619057/141‑1 (MQ=255)
                                                                                                                        aCATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGATCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACGGAAAATGGTTATGGTaaa                   >  1:576545/1‑141 (MQ=255)
                                                                                                                         cATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTAAAATTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAAc                  >  1:166446/1‑141 (MQ=255)
                                                                                                                          aTTACGTCCGTTAGGCCATACAGCAACGGGTGGGAAAGGTATTACACTTCGTGAAGGGGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACg                 <  1:491645/141‑1 (MQ=255)
                                                                                                                           ttACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGt                <  1:168725/141‑1 (MQ=255)
                                                                                                                                 ccTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATCGTAAACGTACGCCa          <  2:590538/141‑1 (MQ=255)
                                                                                                                                  cTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAg         >  2:686460/1‑141 (MQ=255)
                                                                                                                                     tAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCGGTTa      >  2:733725/1‑141 (MQ=255)
                                                                                                                                      aGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTaa     >  1:345530/1‑141 (MQ=255)
                                                                                                                                       ggCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAAt    >  2:760687/1‑141 (MQ=255)
                                                                                                                                        gCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAGATGGTTATGGCAAACGTACGCCAGTTAAta   >  2:625354/1‑140 (MQ=255)
                                                                                                                                         ccGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTCGCTGCAGTATTAGTGGTTACTGAAAATGGTTATGCTAAACCTACGCCACTTAAtta  >  2:255387/1‑139 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GGTAAGATTGCGATTTCGTTCAGAGAAGATGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGA  >  CP000730/8946‑9223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: