Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 396,540 G→A 100% A208T (GCA→ACA)  USA300HOU_0364 → possible lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730396,5400GA100.0% 218.8 / ‑6.5 71A208T (GCA→ACA) USA300HOU_0364possible lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (47/23);  minor base C (1/0);  total (48/23)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CATTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAGTCA  >  CP000730/396403‑396677
                                                                                                                                         |                                                                                                                                         
cATTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAAGTAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGATCGTTGAAGGCacac                                                                                                                                        <  2:588545/141‑1 (MQ=255)
        atCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAAtt                                                                                                                                >  1:726062/1‑141 (MQ=255)
          cATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAAtttt                                                                                                                              >  2:96733/1‑140 (MQ=255)
          cATACCAAAATTAATGTTACAAGGTTCTCTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGACCACAAAAAATTTa                                                                                                                              >  2:679482/1‑141 (MQ=255)
           aTACCAAAATTAATGTTACAAGGTTCTGTTGAACTATTAAATGAAGTTGCTACTTCTAAAATCACAGGAGAGGAAGAAATTTATTCACATACAGGTGTATATGATTATAAAGCGAACGTTGAATGCAGCCAAAAAATTTAt                                                                                                                             >  2:635242/1‑141 (MQ=255)
             aCCAAAATTAATTCTACAAGGTTCTGTTGACCTATTAAATGAACTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATCCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGa                                                                                                                           <  1:792307/141‑1 (MQ=255)
               cAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACt                                                                                                                         >  1:381602/1‑141 (MQ=255)
               cAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACt                                                                                                                         <  2:508119/141‑1 (MQ=255)
                 aaaTTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTa                                                                                                                       >  2:372463/1‑141 (MQ=255)
                       aTGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTaaa                                                                                                                 <  1:424885/141‑1 (MQ=255)
                       aTGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTaaa                                                                                                                 <  1:689230/141‑1 (MQ=255)
                        tGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAAc                                                                                                                >  2:800480/1‑141 (MQ=255)
                         gTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAAct                                                                                                               >  2:657598/1‑140 (MQ=255)
                              aaGGTTCTGTTGACTTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTAttt                                                                                                          <  2:756888/141‑1 (MQ=255)
                                ggTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAAGGCGCAAGTCACACAAAAAATTTATGACTTATTTAAACCTATTTTa                                                                                                        >  1:586060/1‑141 (MQ=255)
                                ggTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATGTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTa                                                                                                        >  1:495961/1‑141 (MQ=255)
                                   tCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTaaaa                                                                                                     >  2:193474/1‑138 (MQ=255)
                                         gACCTATTAAATGAAGTTGCAACTTTTAAAATAACAAGTAAAGAAAAAATTTATACACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCAAATAAAAAGaaaaaa                                                                                               <  2:486875/141‑1 (MQ=255)
                                           ccTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGa                                                                                             <  1:43449/141‑1 (MQ=255)
                                                   aTGAAGTTGCAACTTCTAAAATAACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAAt                                                                                     <  1:423626/141‑1 (MQ=255)
                                                   aTGAAATTGCAACCTCTAAAATCCCAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAAt                                                                                     <  1:543261/141‑1 (MQ=255)
                                                        gTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAgg                                                                                >  2:719851/1‑140 (MQ=255)
                                                          tGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGtga                                                                              >  1:271951/1‑141 (MQ=255)
                                                             aaCTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGtgatga                                                                           <  2:332942/141‑1 (MQ=255)
                                                              aCTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTgatgat                                                                          >  1:536914/1‑141 (MQ=255)
                                                               cTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTAATGata                                                                         >  2:500240/1‑141 (MQ=255)
                                                               cTTCTAAAATCACAGATGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAAATTATGACGTATTTAAACCTATTTTATACACAAACGGTAAAAAATTAAGTAATGata                                                                         >  1:638928/1‑141 (MQ=255)
                                                                 tCTAAAATCACAGGTGAAGAAGAAATATATGCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATc                                                                       <  1:655936/141‑1 (MQ=255)
                                                                  cTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATcc                                                                      >  1:26323/1‑141 (MQ=255)
                                                                    aaaaTCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCaa                                                                    >  2:35313/1‑141 (MQ=255)
                                                                      aaTCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCaaag                                                                  >  1:220364/1‑140 (MQ=255)
                                                                         cacaGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAGAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATGTTAGAGAAAAAAGATAAAAAATTAAATGATAATATACAAATGaa                                                               >  2:665505/1‑141 (MQ=255)
                                                                         cacaGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGaa                                                               >  2:774704/1‑141 (MQ=255)
                                                                          acaGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAAc                                                              >  2:553271/1‑141 (MQ=255)
                                                                           aaGGTGAAGAAGAAATTTATTCACATAAAAATTTATATGATTTTAAAGCGAACGTTAAAGGCAAACAAAAAATTTATGACTTATTTAAACCTATTTTAGAAAAAAAAAAAAAAAAATTAAGTGATGATATCCAAATGAACt                                                             <  2:161386/140‑1 (MQ=255)
                                                                              gTGCAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCg                                                          >  1:737019/1‑141 (MQ=255)
                                                                               tGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTAGa                                                         >  2:807114/1‑141 (MQ=255)
                                                                               tGAAGAAGAAATTTATTCACATACAGATTTATATGATATTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGGGAAAAAAGATAAAAAATAAAGTGATGATATCCAAATGAACTTCGa                                                         >  2:143232/1‑141 (MQ=255)
                                                                                 aagaagaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATa                                                       <  2:818850/141‑1 (MQ=255)
                                                                                   gaagaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATaaa                                                     <  1:800480/141‑1 (MQ=255)
                                                                                      gaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTg                                                  >  1:840504/1‑141 (MQ=255)
                                                                                        aaTTTATTCACATACAGATTTATATGATTTTAAAGCGAATGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGaa                                                >  2:697935/1‑141 (MQ=255)
                                                                                          tttATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATc                                              >  1:397342/1‑141 (MQ=255)
                                                                                          tttATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATc                                              >  2:759417/1‑141 (MQ=255)
                                                                                          tttATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATc                                              >  2:110853/1‑141 (MQ=255)
                                                                                            tATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCaa                                            >  1:281424/1‑141 (MQ=255)
                                                                                             aTTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAat                                           >  2:777780/1‑141 (MQ=255)
                                                                                                 acaTACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTACACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATTAAATGACTCAattagt                                       >  2:623865/1‑139 (MQ=255)
                                                                                                  caTACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATGATTg                                      >  1:244706/1‑141 (MQ=255)
                                                                                                  aacaCAGATTTATAAGATTTTAAAACGAAATTAGAACGCACAAAAAAAATTTATGATTTATTTAAACCTATTCTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTTAATCAATTATTg                                      <  2:239348/138‑1 (MQ=255)
                                                                                                        gATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATaaa                                >  2:705981/1‑141 (MQ=255)
                                                                                                         aTTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAat                               >  2:861960/1‑141 (MQ=255)
                                                                                                            tataTGATTTTAAAGCGAACGTTGAAGGCACACAAAACAATTATTCCTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCACTTATTGGATAAAtata                            >  1:379124/1‑141 (MQ=255)
                                                                                                                tGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGa                        >  1:143635/1‑141 (MQ=255)
                                                                                                                tGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGa                        >  1:309035/1‑141 (MQ=255)
                                                                                                                     gtaaaaggAACGTTGAAAGCACACAAAAAATTGATGACTTATTTAAAGCAATAAAAAAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATaaca                   <  2:785712/134‑1 (MQ=255)
                                                                                                                      tAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATaacaa                  <  1:656945/141‑1 (MQ=255)
                                                                                                                      tAAAGCAAACGTTGAAGCCACACAAAAAATTGATGACTTATTAAAACCGATTTTAGAGAAAAAAGATAAAAAATTAAGTTATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATaacaa                  <  2:764282/141‑1 (MQ=255)
                                                                                                                       aaaGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATaacaac                 >  2:736483/1‑141 (MQ=255)
                                                                                                                       aaaGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATaacaac                 >  1:785465/1‑141 (MQ=255)
                                                                                                                        aaGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcg                >  1:426794/1‑141 (MQ=255)
                                                                                                                         aGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCCATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcgg               <  2:834041/141‑1 (MQ=255)
                                                                                                                          gCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcggc              >  1:711441/1‑141 (MQ=255)
                                                                                                                             aaCGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGGTAAAAATAAAGATAACAACGGCGGt           <  2:397342/141‑1 (MQ=255)
                                                                                                                               cggtGAAGGCAAACATAAAATTTATGACTTATTTAAACTTGTTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTa         <  2:514334/138‑1 (MQ=255)
                                                                                                                                gTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATAATTGAATAAATATAAAGATATCAACGGCGGTTaa        >  2:622830/1‑140 (MQ=255)
                                                                                                                                 ttGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATg       >  2:824539/1‑141 (MQ=255)
                                                                                                                                 ttGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGCCGTTTATg       >  2:189840/1‑141 (MQ=255)
                                                                                                                                 ttGAAGGCACACAAAAAATATATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACATCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATg       >  1:482865/1‑141 (MQ=255)
                                                                                                                                  tGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGa      >  1:824131/1‑141 (MQ=255)
                                                                                                                                   gAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAg     <  2:381602/141‑1 (MQ=255)
                                                                                                                                      ggCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAGTCa  <  1:132364/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
CATTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAGTCA  >  CP000730/396403‑396677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: