Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,969 G→A 100% intergenic (+1027/‑107) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,9690GA98.9% 261.1 / ‑4.7 86intergenic (+1027/‑107)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (51/34);  minor base C (1/0);  total (52/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGTCA  >  CP000730/76837‑77102
                                                                                                                                    |                                                                                                                                       
gATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAAtt                                                                                                                                 >  2:627152/1‑141 (MQ=255)
gATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAAtt                                                                                                                                 <  1:665895/141‑1 (MQ=255)
 aTCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAAttt                                                                                                                                >  2:119870/1‑141 (MQ=255)
  tCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTACTTta                                                                                                                               >  2:821744/1‑141 (MQ=255)
    tACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATATTATACATGGGCTTAAAAGGTTAATTtata                                                                                                                             <  2:835621/141‑1 (MQ=255)
     aCGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCCAGTTACGAGTAAACTGAAATGATAATATTGAGGTTTGTTCTATTTTTTGACTTTGCAATTGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTtatat                                                                                                                            >  2:633838/1‑141 (MQ=17)
      cGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCACCTGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTtatata                                                                                                                           >  1:371340/1‑141 (MQ=255)
         gttgtcgcatggCCGCGTAATTAGCATCATGCTAGCAAGTTAATCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATGTATATAGTa                                                                                                                        <  2:370927/130‑1 (MQ=17)
          ttttCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTAt                                                                                                                       <  1:274902/141‑1 (MQ=255)
          ttttCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTAt                                                                                                                       <  1:864847/141‑1 (MQ=255)
            ttCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTa                                                                                                                     >  2:211816/1‑141 (MQ=255)
            ttCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTa                                                                                                                     >  2:437235/1‑141 (MQ=255)
                ccctgcacagAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGc                                                                                                                 <  1:537449/132‑1 (MQ=255)
                  acgacACGACACTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTTGTGGTTAGCTATATTTTCTTACTTTGCAACAGATCCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGAATTACTGaaa                                                                                                               <  2:192765/137‑1 (MQ=18)
                  aTGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCaa                                                                                                               <  2:864593/141‑1 (MQ=255)
                  aTGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCaa                                                                                                               <  2:657347/141‑1 (MQ=255)
                     ccACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACa                                                                                                            <  2:758693/141‑1 (MQ=255)
                      cACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACAt                                                                                                           <  1:151880/141‑1 (MQ=255)
                          aaaTTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTCGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGAt                                                                                                       >  1:843527/1‑141 (MQ=255)
                           aaTTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGAtt                                                                                                      >  2:508984/1‑141 (MQ=255)
                             ttAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTg                                                                                                    <  1:5633/141‑1 (MQ=255)
                              tAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTgg                                                                                                   >  1:504214/1‑141 (MQ=255)
                               aGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACCGCAAACATTGATATggg                                                                                                  >  2:418554/1‑141 (MQ=255)
                                 catcatGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTc                                                                                               <  2:474698/141‑1 (MQ=255)
                                         tAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACTTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTa                                                                                       >  2:130393/1‑141 (MQ=255)
                                          aGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAg                                                                                      >  1:725542/1‑141 (MQ=255)
                                          aGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAg                                                                                      >  2:499998/1‑141 (MQ=255)
                                           gCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGTTCAAAAATTAgg                                                                                     >  2:423697/1‑141 (MQ=255)
                                                  aaGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTCTAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTa                                                                             >  2:483593/1‑141 (MQ=255)
                                                  aaGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGATCAAAAATTAGGGGTATTa                                                                             >  2:370975/1‑141 (MQ=255)
                                                  aaGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACATATAAAAAACATGGGATTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTAtga                                                                             >  2:626498/1‑139 (MQ=255)
                                                    gCGAACACTGCCATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTTCAAACATTGATTTGGGTCAAAAATTAGGGGTATTaaa                                                                           <  1:379585/141‑1 (MQ=255)
                                                    gCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACATAAAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTaaa                                                                           <  2:723912/141‑1 (MQ=255)
                                                        acacTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTaaaaaaa                                                                       >  1:795549/1‑141 (MQ=255)
                                                             gACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGATTAAAAATTCGGGGTATTAAAAAaatgaat                                                                  >  1:326548/1‑141 (MQ=255)
                                                             gACAGGATAAATTAGTGGTTAGCTATATTTCTTTTCTTTTCTTCAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTAATATAGTATTGCTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAaatgaaa                                                                  >  2:524696/1‑140 (MQ=255)
                                                              aCATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAGCAGAACCTATAAAAAACAAGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACAATGATTTGGGTCAAAAAATAGGAGTTTTAAAGAaatgcata                                                                 >  1:62162/1‑140 (MQ=37)
                                                              aCATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAaatgaatg                                                                 >  1:724615/1‑141 (MQ=255)
                                                                aTGAGAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAACAGGTTAATTTATATAGTATTAATACCAACATTGCTTTGGTTCAAAGATTACGGGTATTaaaaaaagaaattaa                                                               >  2:523495/1‑133 (MQ=25)
                                                                  gATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATc                                                             <  1:288281/141‑1 (MQ=255)
                                                                   aTAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCa                                                            <  1:474040/141‑1 (MQ=255)
                                                                   aTAAATGAGTGGTGAGTGATGTTTTTTTACTTTGCAAGCGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTAAAAAATTAGGGGTATTAAAAAAATGAATGAATCa                                                            <  1:821744/141‑1 (MQ=255)
                                                                     aaaTTAGTGGTTAGCTATATTTTTTTTTTTTTTTTTAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGATTcttt                                                          >  2:415668/1‑138 (MQ=255)
                                                                     aaaTTAGCGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATa                                                          >  1:786031/1‑141 (MQ=255)
                                                                      aaTTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATaa                                                         >  1:48275/1‑141 (MQ=255)
                                                                       atgaGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAAt                                                        <  2:531626/138‑1 (MQ=255)
                                                                            tGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTAt                                                   >  1:638662/1‑141 (MQ=255)
                                                                               ttAGCTATATTTTTTTATTTTTTTACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGATTGAATCATAATTTTATCaa                                                >  2:776298/1‑141 (MQ=255)
                                                                               ttAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCaa                                                >  1:66222/1‑141 (MQ=255)
                                                                                tAGCTATATTTTTTTTTTTTTTTTTAGAACCTATAAAAAACAAGAGCTTAAAAGGTTAATTATTGGAGTATTACTGCAAACATTGCGTTGGGACAAATATTAGGGGTATTAAAAATATGATTGATTCATAATTTTATCagg                                               >  2:814056/1‑139 (MQ=255)
                                                                                tAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTACAAAAATGAATGAATCATAATTTTATCAAg                                               >  1:744061/1‑141 (MQ=255)
                                                                                tAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAg                                               >  1:744120/1‑141 (MQ=255)
                                                                                   cTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAACGGTTACTTTATATAGTATTACTGCAACCATTGATTTGGGTCAAACATTCGGGGTATTAAAAAACTGAATGAATCATACATTTCTCAAGCTg                                            >  1:826405/1‑141 (MQ=255)
                                                                                   cTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTg                                            >  2:508029/1‑141 (MQ=255)
                                                                                   cTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTg                                            <  1:148208/141‑1 (MQ=255)
                                                                                   cTATATCTTTTTACTTTGCAAAAGAACCTAAAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTg                                            <  1:576002/141‑1 (MQ=255)
                                                                                        tttttttACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGCGACCACGAGTATTGATAAAAACACTAAATGAATCATAATTTTATCAAGCTGATTgg                                       >  1:195299/1‑141 (MQ=255)
                                                                                           ttttACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGgaga                                    >  1:14219/1‑141 (MQ=255)
                                                                                             ttACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAgg                                  >  1:211539/1‑141 (MQ=255)
                                                                                             ttACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATCTTATGAAGCTGATTGGAGAgg                                  <  1:442629/141‑1 (MQ=255)
                                                                                               aatttGCAAGAAAACCTATAAAAAACAAGGGCTTAAAACGTTAAATTATATCCTATTACTACAAACATTGATTTGGGATCACCAAAAACTCAATTAAAAAAATGCTTTAATCATAATTTTATCAAGCTGATTGGTGATGtt                                <  2:393299/139‑1 (MQ=255)
                                                                                                cTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTa                               >  1:746514/1‑141 (MQ=255)
                                                                                                  ttGCACCAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTCTCTAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTCTTAAAAAAATGCATGAATCATAATTTTATCAAGCTGATTGgagacgataga                             >  2:720824/1‑134 (MQ=255)
                                                                                                  ttGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTaaa                             >  2:574258/1‑141 (MQ=255)
                                                                                                    gCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAAt                           >  1:67076/1‑141 (MQ=255)
                                                                                                       aCAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATCTAGTGTTACGGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCa                        <  1:492593/141‑1 (MQ=255)
                                                                                                       aCAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCa                        >  2:514764/1‑141 (MQ=255)
                                                                                                        cAGAACCTATAAAAAATATGGGTCTAAAAGGTTAATTTATATAGTATTACGGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCAt                       <  2:827379/141‑1 (MQ=255)
                                                                                                           aaCCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATtat                    >  1:325514/1‑141 (MQ=255)
                                                                                                           aaCCGATAAAAAGCATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGGCAAAAATTAGGGGTATTAAAAAAATGAATGAGTCATAGTTTTATCAAGCTGAATGGAGACGTTAAAATGCATtat                    >  1:610608/1‑141 (MQ=255)
                                                                                                            aCCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATtata                   >  1:758562/1‑141 (MQ=255)
                                                                                                             ccTATAAAAAACGTGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATtatat                  <  2:217970/141‑1 (MQ=255)
                                                                                                             ccTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATtatat                  <  2:48275/141‑1 (MQ=255)
                                                                                                              cgacagaaaaGATGGGATTAAAAGATTAATTTATATAGTACTACTGCAAACAATGAAAAGAAAAAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATtatata                 <  2:343412/135‑1 (MQ=255)
                                                                                                                 taAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATaaaa              >  1:506142/1‑141 (MQ=255)
                                                                                                                   aaaaaCATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAAtt            >  2:159999/1‑141 (MQ=255)
                                                                                                                      aaCATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTAt         <  2:725542/141‑1 (MQ=255)
                                                                                                                       aCATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGTGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAAAGGTTACAATGCATTATATAAAATTTAtt        >  2:219564/1‑141 (MQ=255)
                                                                                                                       aCATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTAtt        <  1:211816/141‑1 (MQ=255)
                                                                                                                          tGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAg     <  2:66222/141‑1 (MQ=255)
                                                                                                                           gggCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGt    >  1:708006/1‑141 (MQ=255)
                                                                                                                           gggCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGt    >  2:284900/1‑141 (MQ=255)
                                                                                                                            ggCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGTc   <  1:23797/141‑1 (MQ=255)
                                                                                                                            ggCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGTc   >  2:338310/1‑141 (MQ=255)
                                                                                                                             gCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAATTGCATTATATAAAATTTATTGAGTCa  <  1:725915/141‑1 (MQ=255)
                                                                                                                             gCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGTCa  <  1:22540/141‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                       
GATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGTCA  >  CP000730/76837‑77102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: