| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,648,469 | T→A | 8.1% | G79G (GGA→GGT) | lltB ← | MFS family major facilitator transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,648,469 | 0 | T | A | 8.1% | 190.9 / 2.8 | 67 | G79G (GGA→GGT) | lltB | MFS family major facilitator transporter |
| Reads supporting (aligned to +/- strand): ref base T (33/28); new base A (4/2); total (37/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATCTGTACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACTTCTACCAAGTACAATTTGCATAACTGCAACGATAAATATAAAACCGCCAACTGTCGCATTTATCCAAAATAGTGGTACTTGAAAATCG > CP000730/2648330‑2648604 | tATCTGTACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTc < 1:506511/141‑1 (MQ=255)tATCTGTACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTc < 2:232889/141‑1 (MQ=255) aTCTGTACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGATTAATACCGCAAACAATTGCTTGAAATAACCTTCATATAGCACAAAATATAAAGTGATGTGCCACTGCACAACTAAAGCTAACTATGATAACTAttgc > 2:752351/1‑139 (MQ=255) aTCTGTACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTcc > 1:253062/1‑141 (MQ=255) aTCTGTACTTAGTTAGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCGGCAGCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTcc > 1:452188/1‑141 (MQ=255) tCTGTACGTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCGCAACCAATTGTTTGAAATAACCTTGATATAGCAAATAATATAAAGCTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTccc < 2:250949/141‑1 (MQ=255) tACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATc < 2:270613/141‑1 (MQ=255) ttAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCg < 1:751341/141‑1 (MQ=255) tAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATAATTACTATTCCCAATccgtt < 1:343961/141‑4 (MQ=255) tttGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCGCAACCAATTGCTTGAAATCACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCAAAAATACAGCAAGATCTCATTAATCTTCCCAATACCATTaa > 2:455581/1‑141 (MQ=255) tGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTaaaa > 1:229355/1‑141 (MQ=255) tGGCAAATAAAGTTAAAGTCACGAGAGGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTaaaa < 1:645298/141‑1 (MQ=255) ggCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCGCAACCACTTGCTTGAAATAACCTTGATATAGCAAATAATATAAATTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAg < 1:126261/141‑1 (MQ=255) ggCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAg < 1:576908/141‑1 (MQ=255) gCTAATAAAGTTAAAGTCACGAGTGGAATAAATCCGACACTAATTGCTTGAAATAACCAAGATATAGCAAAAAATATATAGTTATGTGGCACGGGACACATAAAGCTCGATATGATTACTCTTAACAATCCACTTAAAAga > 2:45835/1‑140 (MQ=255) gCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGt > 2:813789/1‑141 (MQ=255) taataaAGTTAACGTCACGAGAGGAATAATTCCGCAACTATTTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTAc < 2:511769/141‑1 (MQ=255) taataaAGTTAAAGTCACGAGAGGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGCTAAAAGTAc < 1:635232/141‑1 (MQ=255) taataaAGTTAAAGTCACGAGAGGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATCATTACTATTCCCAATCCCGTTAAAAGTAc < 2:512025/141‑1 (MQ=255) caaaagttaaagtCACGAGAGGAATAATTCCACAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCATTAAAAGTACtt < 2:527190/139‑1 (MQ=255) taaagttaaagtCACGAGAGGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACttt > 1:724870/1‑141 (MQ=255) taaagtCACGAGAGGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGa > 2:445599/1‑141 (MQ=255) aaagtCACGAGAGGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGaa > 1:816165/1‑141 (MQ=255) agtCACGAGAGGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATAGCCAATCCCGTTAAAAGTACTTTTTTCGATTc > 1:106641/1‑141 (MQ=255) agtCACGAGAGGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATACCCAATCCCGTTAAAAGTACTTTTTTCGAATc > 1:493112/1‑141 (MQ=255) cGAGAGGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACtttttttttttttttat > 2:779147/1‑130 (MQ=255) cGAGAGGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACgag < 2:660689/141‑1 (MQ=255) agagGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGaa > 2:772713/1‑141 (MQ=255) agagGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGAGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAACGTACTTTTTTCGAATCACGAGaa > 2:454796/1‑141 (MQ=255) acgAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATc < 2:363598/139‑1 (MQ=255) gggacaatGCCGCAACCAATTGCTTGAAATAACCCTTATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATGACTATTCCCAATCCCGTTAAAAGTACTGTTTTCGAATCACGAGAATCt < 2:283290/136‑1 (MQ=255) ggAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATCAAGGTCTGTGTCACTGACCAAATAAAGCTAGATATCATTACTATTACAAATCACATTAAAAGTAGTTGGGTCGAATCACCGGAAtat > 1:390012/1‑139 (MQ=255) gAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCta < 1:543956/141‑1 (MQ=255) gAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCta > 2:830481/1‑141 (MQ=255) gAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCta < 1:264510/141‑1 (MQ=255) gAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGATAGATATGATTACTATTCCCAATCCCGTTAAAAGTCCTTTTTTCGACTCCCGGGAAtata > 1:32204/1‑141 (MQ=255) aacaatTCCACAACCAATTCCTTGAAATAACCTTGATATATCAAATAATACAAAGTTATGTGTCACTGCACAAATACAACTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCtat < 2:4247/138‑1 (MQ=255) gCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATCTGTCGCGTCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACTTCTAcc < 1:812367/141‑1 (MQ=255) aaCCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACTTCTACCaa > 2:663871/1‑141 (MQ=255) aTTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACTTCTACCAAGTACa > 2:565775/1‑141 (MQ=255) cTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACTTCTACCAAGTACAAttt < 2:248407/141‑1 (MQ=255) tGAAATAACCTAGATATAGCACATCATCTAAAGCTATAAGTCACTGCACAGATAAAGCTAGATATGATAACTCTACCCACTCCCGTTACAAGTACTTTTTTCGAATCACGCGAATCTATACTTCCACCAAATACAAtttcc > 2:219291/1‑139 (MQ=255) ccTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCAATACTTCTACCAAGTACAATTTGCATAACTGc > 1:479612/1‑141 (MQ=255) ttGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACTTCTACCAAGTACAATTTGCATAACTGCaa > 2:90884/1‑141 (MQ=255) taGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACTTCTACCAAGTACAATTTGCATAACTGCAACGATaa < 2:509413/141‑1 (MQ=255) cAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTTTTTTTTTTTTTATCTAAAATTATACCAAGAACAATTTGCATGACTGCAAGGATaaaaa > 2:611596/1‑139 (MQ=255) tataAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACTTCTACCAAGTACAATTTGCATAACTGCAACGGTAAATATAAAAcc > 1:367882/1‑141 (MQ=255) aaaGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTTTTTTTTTTTGAATCTCTACTCCTCCTAAGTAAAATTTGCATGACTCCAGCGTTCATTATGAATCCGcc > 2:704852/1‑141 (MQ=255) ttgttgcccctcacaAATATAGATAGATATGCTTACTAGTCCCAGTCCCGTTAAAAGTACTTTTTTCGAGTCACTACCATCTATACTTCTACCAAGTACACTTTGCATAACTGCAACGATAAATATAAAACCGCCAACTGt < 2:468286/130‑1 (MQ=255) tgtgTCACTGCACCAATAAAGCTAGATATGATTACCAGTCCTAATCACGTTAAAAGTACTTATTTCGAATCACGCGAATCTATCCTTCGACCAGGCACAATTTGCATAACTAACACGATAAATATAAAACCGACAACTGTc > 2:388483/1‑141 (MQ=255) gtgtaaatgaaaaaaTAAAGCTAAATATGATTACTATACCCAATCCCGTCAAAAGTACTTTTTTCGAATCACGAGAATCTATACTTCTACCAAGTACAATTTGCATAACTGCAACGATAAATATAAAACCGCCAACTGTCg < 2:106641/129‑1 (MQ=255) gtgtCACTGCACAAATAAAGCTAGATATGATTACTATACCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACTTCTACCAAGTACAATTTGCATAACTGCAACGATAAATATAAAACCGCCAACTGTCg < 2:493112/141‑1 (MQ=255) tgtCACTGCACAAATAAAGCTAGGTATGATTACTATTCCCAACCCCGTTAAATGTACTTTTTTCGAATCACGAGAATCTATACTTCTACCAAGTACAATTTGCATAACTGCAACGATAAATATAAAACCGCCAACTGTCGc < 2:914/141‑1 (MQ=255) tgtCACTGCACAAATAAAGCTAGATATGATAAAACAAAAAAAAACCGTTAAAAGTACTTTTTTGGAAGCACGAGAATCTATACTTCTACCAAGTACAATTTGCATAACTGCACCGATAAAAACAAAACCGCCacccgttgc > 1:508800/1‑133 (MQ=255) tCATTGCACAAATAAAGCTCGAGTTGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTAGAATTCTACCAAGTACAATTTGCATAACTGCAACGATAAATATAAAACCGCCAACTGTCGCAt < 2:210251/141‑1 (MQ=255) aCTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACTTCTACCAAGTACAATTTGCATAACTGCACCGATAAATATAAAACCGCCAACTGTCCCAttt > 1:341629/1‑141 (MQ=255) aCTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACTTCTACCAAGTACAATTTGCATAACTGCAACGATAAATATAAAACCGCCAACTGTCGCAttt > 1:341657/1‑141 (MQ=255) aaaTAAAGCTAGATATGATTCCTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCTCGAGAATCTATACTTCTACCAAGTACAATTTGCATAACTGCAACGATAAATATAAGACCGCCAACTGTCGCATTTATCCaaa > 1:527210/1‑141 (MQ=255) aaTAGAGCTAGATATGATTACTATTCCCAATCCCGTTAACAGTACTTTTTTCGAATCACGAGAATCTATACTTCTACCAAGTACAATTTGCATAACTGCAACGATAAATATAAAACCGCCAACTGTCGCATTTATCCaaaa < 2:483691/141‑1 (MQ=255) aaaGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACTTCTACCAAGTACAATTTGCATAACTGCAACGATAAATATAAAACCGCCAACTGTCGCATTTATCCAAAATAg > 1:825684/1‑141 (MQ=255) aGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACTTCTACCAAGTACAATTTGCATAACTGCCACGATAAATGTTAAACCGCCAACTGTCGCATTTATCCAAAATAGTg > 1:585991/1‑141 (MQ=255) tAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACTTCTACCAAGTACAATTTGCATAACTGCAACGATAAATATAAAACCGCCAACTGTCGCATTTATCCAAAATAGTGGTa > 1:457448/1‑141 (MQ=255) atatGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACTTCTACCAAGTACAATTTGCATAACTGCATCGATAAATACAAAACCGCCAACTGTCGCATTTATCCAAAATAGTGGTACtt < 1:593053/141‑1 (MQ=255) tatGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACTTCTACCAAGTACAATTTGCATAACTGCAACGATAAATATAAAACCGCCAACTGTCGCATTTATCCAAAATAGTGGTACttt > 2:469456/1‑140 (MQ=255) tatGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACTTCTACCAAGTACAATTTGCATAACTGCAACGATAAATATAAAACCGCCAACTGTCGCATTTATCCAAAATAGTGGTACTTg < 1:813789/141‑1 (MQ=255) tGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACTTCTACCAAGTACAATTTGCATAACTGCAACGATAAATATAAAACCGCCAACTGTCGCATTGATCCAAAATAGTGGTACTTGaa > 1:686194/1‑141 (MQ=255) tACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACTTCTACCAAGTACAATTTGCATAACTGCAACGATAAATATAAAACCGCCAACTGTCGCATTTATCCAAAATAGTGGTACTTGaaaacc > 2:864969/1‑139 (MQ=255) aCTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACTTCTACCAAGTACAATTTGCATAACTGCAACGATAAATATAAAACCGCCAACTGTCGCATTTATCCAAAATAGTGGTACTTGAAAATCg > 1:746173/1‑141 (MQ=255) | TATCTGTACTTAGTTTGGCTAATAAAGTTAAAGTCACGAGAGGAATAATTCCGCAACCAATTGCTTGAAATAACCTTGATATAGCAAATAATATAAAGTTATGTGTCACTGCACAAATAAAGCTAGATATGATTACTATTCCCAATCCCGTTAAAAGTACTTTTTTCGAATCACGAGAATCTATACTTCTACCAAGTACAATTTGCATAACTGCAACGATAAATATAAAACCGCCAACTGTCGCATTTATCCAAAATAGTGGTACTTGAAAATCG > CP000730/2648330‑2648604 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |