Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,405,4890GT77.8% 78.1 / 25.7 45intergenic (‑127/‑124)USA300HOU_2268/ureAUT family urea transporter/urease gamma subunit
Reads supporting (aligned to +/- strand):  ref base G (7/3);  new base T (18/17);  total (25/20)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAATCTTTCACCTTTCCTTTGAATCATTACTCATTAATATACTACTATTCTATGAAAAGTTATATTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCGGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATCACAACAATTAGGATTAATATAAATTTTGTAAATTGTATCTAATATGAAATTGGGGGTTTTAATTTGCATTTTACAC  >  CP000730/2405352‑2405625
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aactCTTTCACCTTTCCTTTGAATCATTACTCATTAATATACTCCTCTTCTATGAAAAGTTATATTAAAAACTATTTTGGTTTTTACATACATATATTCCACTATTTGTTTTCGATTAGCTTGTTTGCTTCAATATCTGat                                                                                                                                       <  2:61779/138‑1 (MQ=255)
    cTTTCACCTTTCCTTTGAATCATTACTCATTAATATACTACTATTCTATGAAAAGTTATATTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGc                                                                                                                                   >  1:380039/1‑141 (MQ=255)
       tCACCTTTCCTTTGAATCATTACTCATTAATATACTACTATTCTATGAAAAGTTATATTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATATGATATGCTaa                                                                                                                                >  1:276692/1‑141 (MQ=255)
          cctttcctttGAATCATTACTCATTAATATACTACTATTCTATGAAAAGTTATATTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGCTTAGATTGTTTGCTTCAATATCTCATATTCTaaaaa                                                                                                                             >  2:246328/1‑141 (MQ=255)
          cctttcctttGAATCATTACTCATTAATATACTACTATTCTATGAAAAGTTATATTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGCTaaaaa                                                                                                                             <  2:187921/141‑1 (MQ=255)
              tcctttGAATCATTACTCATTAATATACTACTATTCTATGAAAAGTTATATTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCGGATATGCTAAAAAtata                                                                                                                         >  2:410703/1‑141 (MQ=255)
               cctttGAATCATTACTCATTAATATACTACTATTCTATGAAAAGTTATATTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGCTAAAAATATaa                                                                                                                        <  1:20771/141‑1 (MQ=255)
                ctttGAATCATTACTCATTAATATACTACTATTCTATGAAAAGTTATATTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAAt                                                                                                                       >  1:306850/1‑141 (MQ=255)
                   tGAATCATTACTCATTAATATACTACTATTCTATGAAAAGTTATATTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAAtttt                                                                                                                    <  1:404059/141‑1 (MQ=255)
                    gAATCATTACTCATTAATATACTACTATTCTATGAAAAGTTATATTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAATTTTc                                                                                                                   >  1:155372/1‑141 (MQ=255)
                          ttACTCATTAATATACTACTATTCTATGAAAAGTTATATTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAATTTTCTAATTa                                                                                                             <  1:303961/141‑1 (MQ=255)
                              tCATTAATATACTACTATTCTATGAAAAGTTATATTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAATTTTCTAATTAcaca                                                                                                         <  2:222541/141‑1 (MQ=255)
                              tCATTAATATACTACTATTCTATGAAAAGTTATATTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAATTTTCTAATTAcaca                                                                                                         <  1:406212/141‑1 (MQ=255)
                               cATTAATATACTACTATTCTATGAAAAGTTATATTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAATTTTCTAATTACACAt                                                                                                        <  2:270843/141‑1 (MQ=255)
                                aTTAATATACTACTATTCTATGAAAAGTTATATTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAATTTTCTAATTACACAtt                                                                                                       <  2:163763/141‑1 (MQ=255)
                                         ctactaTTCTATGAAAAGTTATATTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATGTGTTTTCGATTAGATTGTTTGCTTCAATATCGGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGt                                                                                              >  2:323314/1‑141 (MQ=255)
                                                 cTATGAAAAGTTATATTAAAAACTATTTTGGTTTTTACATAAATATATTTCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACt                                                                                      <  2:90305/141‑1 (MQ=255)
                                                 cTATGAAAAGTTATATTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACt                                                                                      <  2:90342/141‑1 (MQ=255)
                                                   aTGAAAAGTTATATTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCGGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTg                                                                                    >  1:419789/1‑141 (MQ=255)
                                                   aTGAAAAGTTATATTAAAAAATATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTg                                                                                    >  1:118748/1‑141 (MQ=255)
                                                    tGAAAAGTTATATTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGt                                                                                   >  1:262414/1‑141 (MQ=255)
                                                     gAAAAGTTATATTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGtt                                                                                  <  1:293547/141‑1 (MQ=255)
                                                      aaaaGTTATATTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGttt                                                                                 >  1:16501/1‑141 (MQ=255)
                                                           ttATATTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCGGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATcac                                                                            >  2:115989/1‑141 (MQ=255)
                                                              tatTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATCacaac                                                                         >  1:381222/1‑141 (MQ=255)
                                                              tatTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATCacaac                                                                         >  1:311645/1‑141 (MQ=255)
                                                                ttAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCGGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATCAcaacaa                                                                       >  1:304211/1‑141 (MQ=255)
                                                                         aTTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCGGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATCACAACAATTAGGATTa                                                              >  1:394734/1‑141 (MQ=255)
                                                                             tGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATCCCAACAATTAGGATTAatat                                                          >  2:73681/1‑141 (MQ=255)
                                                                                 ttttACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATCACAACAATTAGGATTAATATAAAt                                                      >  2:393779/1‑141 (MQ=255)
                                                                                 ttttACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATCACAACAATTAGGATTAATATAAAt                                                      >  2:404333/1‑141 (MQ=255)
                                                                                    tACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATCACAACAATTAGGATTAATATAAAtttt                                                   <  2:41280/141‑1 (MQ=255)
                                                                                                    aCTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATCACAACAATTAGGATTAATATAAATTTTGTAAATTGTATCTAat                                   >  1:376456/1‑141 (MQ=255)
                                                                                                                 gATTAGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATCACAACAATTAGGATTAATATAAATTTTGTAAATTGTATCTAATATGAAATTggggg                      <  1:301208/141‑1 (MQ=255)
                                                                                                                 gATTAGATTGTTTGCTTCAATATCGGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATCACAACAATTAGGATTAATATAAATTTTGTAAATTGTATCTAATATGAAATTggggg                      <  2:416838/141‑1 (MQ=255)
                                                                                                                     aGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATCACAACAATTAGGATTAATATAAATTTTGTAAATTGTATCTAATATGAAGTTGGGGGtttt                  >  2:281877/1‑141 (MQ=255)
                                                                                                                     aGATTGTTTGCTTCAATATCTGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATCACAACAATTAGGATTAATATAAATTTTGTAAATTGTATCTAATATGAAATTGGGGGtttt                  >  1:131007/1‑141 (MQ=255)
                                                                                                                       tttttttGCTTCAATATCGGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATCACAACAATTAGGATTAATATAAATTTTGTAAATTGTATCTAATATGAAATTGGGGGTTTTaa                <  1:30421/137‑1 (MQ=255)
                                                                                                                           tttGCTTCAATATCGGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATCACAACAATTAGGATTAATATAAATTTTGTAAATTGTATCTAATATGAAATTGGGGGTTTTAATTTg            >  2:264545/1‑141 (MQ=255)
                                                                                                                           tttGCTTCAATATCGGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATCACAACAATTAGGATTAATATAAATTTTGTAAATTGTATCTAATATGAAATTGGGGGTTTTAATTTg            <  2:209072/141‑1 (MQ=255)
                                                                                                                                 tCAATATCTGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATCACAACAATTAGGATTAATATAAATTTTGTAAATTGTATCTAATATGAAATTGGGGGTTTTAATTTGCAtttt      <  2:16501/141‑1 (MQ=255)
                                                                                                                                  cAATATCTGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATCACAACAATTAGGATTAATATAAATTTTGTAAATTGTATCTAATATGAAATTGGTGGTTTTAATTTGCATTTTa     >  2:370423/1‑141 (MQ=255)
                                                                                                                                  cAATATCTGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATCACAACAATTAGGATTAATATAAATTTTGTAAATTGTATCTAATATGAAATTGGGGGTTTTAATTTGCATTTTa     <  2:311645/141‑1 (MQ=255)
                                                                                                                                    atatCTGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATCACAACAATTAGGATTAATATAAATTTTGTAAATTGTATCTAATATGAAATTGGGGGTTTTAATTTGCATTTTaca   >  1:293967/1‑141 (MQ=255)
                                                                                                                                     tatCTGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATCACAACAATTAGGATTAATATAAATTTTGTAAATTGTATCTAATATGAAATTGGGGGTTTTAATTTGCATTTTacac  <  1:195032/141‑1 (MQ=255)
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AAATCTTTCACCTTTCCTTTGAATCATTACTCATTAATATACTACTATTCTATGAAAAGTTATATTAAAAACTATTTTGGTTTTTACATAAATATATTCCACTATTTGTTTTCGATTAGATTGTTTGCTTCAATATCGGATATGCTAAAAATATAATTTTCTAATTACACATTGAACTTTGTATATTACTTGTTTATCACAACAATTAGGATTAATATAAATTTTGTAAATTGTATCTAATATGAAATTGGGGGTTTTAATTTGCATTTTACAC  >  CP000730/2405352‑2405625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑