| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP000730 | 9,085 | G→A | R685H (CGT→CAT) | gyrA → | DNA topoisomerase (ATP‑hydrolyzing) subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 9,085 | 0 | G | A | 82.9% | 73.6 / 10.8 | 35 | R685H (CGT→CAT) | gyrA | DNA topoisomerase (ATP‑hydrolyzing) subunit A |
| Reads supporting (aligned to +/- strand): ref base G (3/3); new base A (13/16); total (16/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01 | |||||||||||
AAGATTGCGATTTCGTTCAGAGAAGATGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACT > CP000730/8949‑9225 | aaGATTGCGATTTCGTTCAGAGAAGATGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACa > 1:49070/1‑141 (MQ=255) tgattttgATTGCGTTCAGAGAAGATGATTAGTTCATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCGTTATTGATACATTTCTTGCATCATTAATTCGATTCCCTCAATCAACATTACCTTCTTTAGGCCATACat < 2:287817/134‑2 (MQ=255) gATTGCGATTTCGTTCAGAGAAGATGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGc < 2:260774/141‑1 (MQ=255) gATTGCGATTTCGTTCAGAGAAGATGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACCGc < 2:94709/141‑1 (MQ=255) gATTGCGATTTCGTTCAGAGAAGATGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGc < 2:94605/141‑1 (MQ=255) cGTTCAGAGAAGATGATGAGTTAATTGCAGTAGGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGACTTAACATTACGTCCTTTAGGCCATACCGCACCGGGTGTGa < 2:250762/141‑1 (MQ=255) tCAGAGAAGATGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAg > 2:161143/1‑141 (MQ=255) gatgatGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTaca < 2:224979/141‑1 (MQ=255) gatgaGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACtt > 2:224021/1‑141 (MQ=255) ttAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGaa > 2:65166/1‑141 (MQ=255) aaTTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAgg < 2:373723/141‑1 (MQ=255) tGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGa < 1:15042/141‑1 (MQ=255) cAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACg < 1:360392/141‑1 (MQ=255) gTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGaa > 2:29029/1‑141 (MQ=255) gCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTg < 1:399942/141‑1 (MQ=255) cTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGt < 1:268646/141‑1 (MQ=255) ggTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGAt > 2:233256/1‑141 (MQ=255) cAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTa > 2:48073/1‑141 (MQ=255) agaagaTATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGc < 1:300877/141‑1 (MQ=255) tCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATAAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGGTGTAGGGCTTGATGTAGCTCATGCaa > 2:285672/1‑141 (MQ=255) aTTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAgt > 2:104948/1‑141 (MQ=255) tGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAgtgt < 2:288915/141‑1 (MQ=255) ggTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGtt > 1:394581/1‑141 (MQ=255) gTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTtg > 2:301826/1‑141 (MQ=255) tACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTtga < 2:289008/141‑1 (MQ=255) aCATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTtgat > 1:35222/1‑141 (MQ=255) tAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTa < 1:301826/141‑1 (MQ=255) cGATTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGAAACGGGTGTGCCAGGTATTAAACTACGTGAAAGTGAAGAAGTAGACGGAAACGACGTAGAAAAGGAAAAAAATGTTGGTGAAGTATTAGTAGTTACTGaa > 1:270728/1‑141 (MQ=255) aTTCCCTGAATCAACATTACGTCCTTTAGGCCATACAGCAACGGGAGCGAAAGGTATTACACTTCGTGAAGGTGACGAGGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGCAGTAGTAGTAGTTACTGaaaa > 1:107157/1‑141 (MQ=255) cTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGtt < 1:233256/141‑1 (MQ=255) aTCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAATGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATgg < 1:202851/141‑1 (MQ=255) cAACATTACGTCCTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTa < 2:258108/141‑1 (MQ=255) ccTTTAGGCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGGGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCa > 1:343076/1‑141 (MQ=255) ggCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAAt < 1:29029/141‑1 (MQ=255) gCCATACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATg > 2:117518/1‑141 (MQ=255) gTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACt < 1:224021/141‑1 (MQ=255) | AAGATTGCGATTTCGTTCAGAGAAGATGATGAGTTAATTGCAGTTCGCTTAACAAGTGGTCAAGAAGATATCTTGATTGGTACATCACATGCATCATTAATTCGATTCCCTGAATCAACATTACGTCCTTTAGGCCGTACAGCAACGGGTGTGAAAGGTATTACACTTCGTGAAGGTGACGAAGTTGTAGGGCTTGATGTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACT > CP000730/8949‑9225 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |