Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 195,286 T→A intergenic (‑97/‑82) USA300HOU_0183 ← / → USA300HOU_0184 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730195,2860TA82.2% 116.7 / 15.4 45intergenic (‑97/‑82)USA300HOU_0183/USA300HOU_0184hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (5/3);  new base A (15/22);  total (20/25)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GTTTCTAGCTAAACTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTTAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGATTTTGCATCAACGAAAGAAG  >  CP000730/195151‑195422
                                                                                                                                       |                                                                                                                                        
gTTTCTAGCTAAACTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTaaaaaa                                                                                                                                     <  1:307639/141‑1 (MQ=255)
   tCTAGCTAAACTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAat                                                                                                                                  >  2:351906/1‑141 (MQ=255)
        cTAAACTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCtt                                                                                                                             <  2:323511/141‑1 (MQ=255)
        atAAACTTGCTACGAAAGATAATATATAAAAAAAGATAGTCCTCTATATATATGTTTATTAACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACATAATTTTATCAAAAAAAAAAAAAAAATATCtt                                                                                                                             <  2:289544/140‑1 (MQ=255)
         tAAACTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACAAGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTa                                                                                                                            <  1:246848/141‑1 (MQ=255)
         tAAACTTGCTACGAAAGATTAATTAAAAATAATGATAGCCTCATATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGTATTTTATCTAAAAATAAAAAAAAATATCTTa                                                                                                                            <  2:352499/141‑1 (MQ=255)
           aaCTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAat                                                                                                                          <  1:304595/141‑1 (MQ=255)
            aCTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTAACTAAAAAATAAAAAAAATATCTTAata                                                                                                                         <  2:300226/141‑1 (MQ=255)
                      aCAGGTTCTTTATCCAGAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAAt                                                                                                               <  2:312703/141‑1 (MQ=255)
                          aTTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTTAAAAAAATATCTTAATATc                                                                                                                       >  2:307456/1‑129 (MQ=255)
                          aTTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTTAAAAAAATATCTTAATATc                                                                                                                       <  1:307456/129‑1 (MQ=255)
                           ttCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGAt                                                                                                          >  2:195917/1‑141 (MQ=255)
                            tCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATAAAAAAAAAAATATCTTAATATCCTTGTAATCCGATa                                                                                                         <  2:67328/141‑1 (MQ=255)
                               ttATCCATAATGATAGCCGCCTATTTATATGGTTTTTTACTTATACCATAACATGCTTTTTATATTCTTTGAAAATATATTTTAAAGATCTTTATCTACATTTTAAAAAAAATCTGCTAATATAATTGTAAGCAGATaata                                                                                                      >  2:53619/1‑139 (MQ=255)
                                tATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTTAAAAAAATATCTTAATATCCTTGTAATCCGATAAGaa                                                                                                     >  2:78615/1‑141 (MQ=255)
                                tATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTTAAAAAAATATCTTAATATCCTTGTAATCCGATAAGaa                                                                                                     >  2:166471/1‑141 (MQ=255)
                                       aaTGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGt                                                                                              >  2:38000/1‑141 (MQ=255)
                                           aTAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTTAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAata                                                                                          <  1:391092/141‑1 (MQ=255)
                                             aGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATa                                                                                                         <  2:263500/124‑1 (MQ=255)
                                             aGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATa                                                                                                         >  1:263500/1‑124 (MQ=255)
                                              gCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATAttt                                                                                       >  2:8277/1‑141 (MQ=255)
                                                    tatatataTGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCaa                                                                                 >  1:64537/1‑141 (MQ=255)
                                                     atatatatGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAAc                                                                                >  1:86109/1‑141 (MQ=255)
                                                         atatGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCAtt                                                                            <  1:390705/141‑1 (MQ=255)
                                                              tttatttaCTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTtat                                                                       <  1:258694/141‑1 (MQ=255)
                                                                      cTTATACCCTAACATGATTTTTATATTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTc                                                               >  1:200792/1‑141 (MQ=255)
                                                                       ttATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCt                                                              <  1:24046/141‑1 (MQ=255)
                                                                         ataCCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCtta                                                            >  2:400899/1‑141 (MQ=255)
                                                                                      aTTTTTATATTTTTTTAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACattat                                               >  2:35004/1‑141 (MQ=255)
                                                                                      aTTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACattat                                               >  1:395918/1‑141 (MQ=255)
                                                                                         tttATACTCTTTGAAATTATATTTTACAGAATTTTATCTAAATATTTAAGAAAGTATGTTTATATCATTGTGATCCGAAAAGAATTATAGTAATATTTTTTCAATCATCGTTAGAGGCGGTATTAATAATGTCATTAgtta                                            <  2:117374/141‑2 (MQ=255)
                                                                                         tttATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTAtttt                                            <  1:276956/141‑1 (MQ=255)
                                                                                           tataCTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTtta                                          <  2:160269/141‑1 (MQ=255)
                                                                                             taCTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTttatt                                        >  2:372552/1‑141 (MQ=255)
                                                                                                tctTTGAAAATATATTTTACAGAATTTTATCTAAATATTAAAAAACATATCTTAATATCCTCATATGCGGATTAAAAATAGGGTAATAGATTGTCAACCATGTTAATAGGAGGGCTTATTAATTACATTATTTTTATTAGa                                     >  1:143293/1‑141 (MQ=255)
                                                                                                           atatTTTACAGAATTTTATCTAAATATTTAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCCACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATc                          >  1:235789/1‑141 (MQ=255)
                                                                                                           atatTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATc                          >  1:155102/1‑141 (MQ=255)
                                                                                                            tatTTTACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCt                         <  2:168359/141‑1 (MQ=255)
                                                                                                                 tACAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGAtt                    <  2:86109/141‑1 (MQ=255)
                                                                                                                  aCAGAATTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGAttt                   <  2:38256/141‑1 (MQ=255)
                                                                                                                  aCAGAATTTTATATAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGAttt                   <  2:38251/141‑1 (MQ=255)
                                                                                                                      aaTTTTATCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGATTTTGCa               <  1:306787/141‑1 (MQ=255)
                                                                                                                            aTCTAAATATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGATTTTGCATCAACg         <  1:351906/141‑1 (MQ=255)
                                                                                                                             tCTAAATATTTAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGATTTTGCATCAACGa        >  2:327619/1‑141 (MQ=255)
                                                                                                                                 aaTATTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTTATTTTGCATCAACGAaaga    <  1:200633/141‑1 (MQ=255)
                                                                                                                                   tatTAAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGATTTTGCATCAACGAaagaag  <  1:372552/141‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
GTTTCTAGCTAAACTTGCTACGACAGATTCTTTATCCATAATGATAGCCCCCTATATATATGTTTATTTACTTATACCCTAACATGATTTTTATACTCTTTGAAAATATATTTTACAGAATTTTATCTAAATATTTAAAAAAATATCTTAATATCCTTGTAATCCGATAAGAATTATAGTAATATTTTTTCAACCATTGTTATAGGAGGTCTTATTAATGACATTATTTTTATTAGAAGCTAACAATCTTGATTTTGCATCAACGAAAGAAG  >  CP000730/195151‑195422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑