Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,268,558:1 +A 6.9% intergenic (‑446/+92) USA300HOU_2142 ← / ← USA300HOU_2143 hypothetical protein/possible HAD superfamily hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,268,5581.A 6.9% 243.2 / 10.1 58intergenic (‑446/+92)USA300HOU_2142/USA300HOU_2143hypothetical protein/possible HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  ref base . (23/31);  new base A (2/2);  total (25/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01

TAGTTAAACCTTAAAACAAGAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCATCCTC  >  CP000730/2268419‑2268697
                                                                                                                                            |                                                                                                                                           
tAGTTAAACCTTAAAACAAGAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTa                                                                                                                                            <  2:715913/141‑1 (MQ=255)
  gTTAAACCTTAAAACAAGAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAg                                                                                                                                          >  1:428894/1‑141 (MQ=255)
       aCCTTAAAACAAGAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACAtt                                                                                                                                     >  1:185240/1‑141 (MQ=255)
       aCCTTAAAACAAGAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACAtt                                                                                                                                     >  1:214945/1‑141 (MQ=255)
             aaaCAAGAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTaa                                                                                                                               <  1:540593/141‑1 (MQ=255)
             aaaCAAGAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTaa                                                                                                                               <  1:540476/141‑1 (MQ=255)
              aaCAAGAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGCGTTTGTCCAATTACATCTAGATGATTCAACTATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAAt                                                                                                                              <  1:78438/141‑1 (MQ=255)
                cAAGAAATATTATTCANAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAg                                                                                                                            >  1:433344/1‑141 (MQ=255)
                 aaGAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGa                                                                                                                           <  1:539667/141‑1 (MQ=255)
                 aaGAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAAAAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGa                                                                                                                           <  1:468670/141‑1 (MQ=255)
                   gAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGa                                                                                                                                         >  1:42360/1‑125 (MQ=255)
                   gAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGa                                                                                                                                         <  2:42360/125‑1 (MQ=255)
                       tattatTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAAGTTATTTTCTATTAGAAAAtg                                                                                                                      >  1:5080/1‑140 (MQ=39)
                         ttattCAAAATCAATGAATTTCGTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACt                                                                                                                   <  2:427650/141‑1 (MQ=255)
                               aaaaTCAATGAATTTCCTATCTTATTAG‑‑TTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAAGTTATTTTCTATTAGAAAAtgtcttactgct                                                                                                            <  2:225651/141‑12 (MQ=25)
                                 aaTCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAAGTTATTTTCTATTAGAAAAtgtcttactgct                                                                                                            <  1:568568/141‑12 (MQ=37)
                                  aTCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGctc                                                                                                          <  2:206829/141‑1 (MQ=255)
                                    cAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCtt                                                                                                        <  2:409266/141‑1 (MQ=255)
                                    cAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCtt                                                                                                        <  1:376043/141‑1 (MQ=255)
                                     aaTGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCttt                                                                                                       >  1:155553/1‑141 (MQ=255)
                                      aTGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTg                                                                                                      >  1:429684/1‑141 (MQ=255)
                                        gAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTa                                                                                                    >  2:560948/1‑141 (MQ=255)
                                          aTTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACa                                                                                                  <  2:508632/141‑1 (MQ=255)
                                             tCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTa                                                                                               <  2:9803/141‑1 (MQ=255)
                                              ccTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTTATAGAAAATAACTTTACTGCTCTTTGTTCATTAc                                                                                              >  2:117299/1‑141 (MQ=255)
                                                tATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACtc                                                                                            <  2:439368/141‑1 (MQ=255)
                                                   cTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACtcatc                                                                                         <  1:329794/141‑1 (MQ=255)
                                                    ttattaGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACtcatca                                                                                        <  2:423608/141‑1 (MQ=255)
                                                       ttaGTTTTAACAATAGTTATTCTCATAGAGCTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACt                                                                                     >  2:776905/1‑141 (MQ=255)
                                                        taGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTa                                                                                    >  2:311468/1‑141 (MQ=255)
                                                           ttttAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTg                                                                                 <  2:462699/141‑1 (MQ=255)
                                                              tAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGtaa                                                                              <  1:358497/141‑1 (MQ=255)
                                                              tAACAACATTTAGTCTAATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGtaa                                                                              <  1:326653/141‑1 (MQ=255)
                                                                    tatttattCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGtaaattaaa                                                                        >  1:392870/1‑141 (MQ=255)
                                                                     atttattCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGtaaattaaat                                                                       <  2:691108/141‑1 (MQ=255)
                                                                         attCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATtaaataata                                                                   <  2:582525/141‑1 (MQ=255)
                                                                          ttCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATtaaataataa                                                                  >  1:261526/1‑141 (MQ=255)
                                                                           tctcATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATtaaataataaa                                                                 <  2:761954/141‑1 (MQ=255)
                                                                             tcATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATtaaataataaata                                                               >  1:273861/1‑141 (MQ=255)
                                                                               aTAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAAc                                                             <  1:344046/141‑1 (MQ=255)
                                                                                tAGAGTTTGTCCACTTAAATATAGATGATTCAAATATATATAAGCACACAAAAGAGCAGTAAGACATTTTCTAATAAAAAATAACTTTACCGCTCTTTGTACATTCTGCAGCAGCTATGGTAAATTAAATAATAAATAACt                                                            >  2:13130/1‑141 (MQ=255)
                                                                                tAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACt                                                            >  2:424685/1‑141 (MQ=255)
                                                                                    gTTTGTCCAATTAAATATAGATGATTAAAATAATAATAAAAAAAAAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCt                                                        <  2:354426/141‑1 (MQ=255)
                                                                                      ttGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTAt                                                      >  1:620458/1‑141 (MQ=255)
                                                                                         tCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTAtttt                                                   >  2:107796/1‑141 (MQ=255)
                                                                                         tCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTAtttt                                                   <  2:484539/141‑1 (MQ=255)
                                                                                                   tataGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGc                                         <  1:288844/141‑1 (MQ=255)
                                                                                                     taGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTa                                       <  2:157320/141‑1 (MQ=255)
                                                                                                                  tatTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGc                          <  1:757402/141‑1 (MQ=255)
                                                                                                                        taAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAata                    >  2:263706/1‑141 (MQ=255)
                                                                                                                        taAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAata                    >  1:27908/1‑141 (MQ=255)
                                                                                                                                acaaaGAGCAGTAAAGTTATTTTCTATTAGAAAATGTCTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTtgctg            >  1:594640/3‑141 (MQ=25)
                                                                                                                                   aaGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTTCCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTgcgc         <  2:392364/141‑1 (MQ=255)
                                                                                                                                    agagCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGcc        >  2:87193/1‑141 (MQ=255)
                                                                                                                                      agCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTCAATATGTTGCTGCGCCAt      >  1:241856/1‑141 (MQ=255)
                                                                                                                                        ccgTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCATcc    <  1:559072/139‑1 (MQ=255)
                                                                                                                                        cAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAGCCGCCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCATcc    <  1:280311/141‑1 (MQ=255)
                                                                                                                                        cAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCATcc    <  2:684422/141‑1 (MQ=255)
                                                                                                                                          gTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCATCctc  >  2:430182/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                           
TAGTTAAACCTTAAAACAAGAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAGACATTTTCTAATAGAAAATAACTTTACTGCTCTTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCATCCTC  >  CP000730/2268419‑2268697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: