BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,862:1	+T	33.8%	intergenic (‑945/‑223)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,862	1	.	T	33.8%	174.0 / 196.3	130	intergenic (‑945/‑223)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (48/38); new base T (15/29); total (63/67)
Fisher's exact test for biased strand distribution p-value = 2.58e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.05e-03

Only 100 of 131 total aligned reads displayed.

TTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTA  >  CP000730/1929722‑1929999

ttAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGta                                                                                                                                               <  1:179272/141‑1 (MQ=255)

    gtgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATGTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑g                                                                                                                                         <  1:177257/141‑1 (MQ=255)

      gtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑t                                                                                                                                      <  1:325832/141‑1 (MQ=255)

       tAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑g                                                                                                                                    >  2:402398/1‑141 (MQ=255)

          aCGATTCAATCTATCAGCTAGTATCATATATCTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATg                                                                                                                                 <  1:69913/141‑1 (MQ=255)

          aCGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATg                                                                                                                                 <  1:590270/141‑1 (MQ=255)

          aCGATTCAATCTATCAGCTAGAAGCATATAACTTACCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATg                                                                                                                                 <  1:267486/141‑1 (MQ=255)

           cGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑Taactgctcta                                                                                                                                   >  1:377444/1‑133 (MQ=255)

            gATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCt                                                                                                                               <  2:1607/141‑1 (MQ=255)

              ttCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTa                                                                                                                             <  2:299697/141‑1 (MQ=255)

                cAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTAtt                                                                                                                           <  2:17928/141‑1 (MQ=255)

                cAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTAtt                                                                                                                           <  2:39716/141‑1 (MQ=255)

                   tCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTc                                                                                                                        <  2:496401/141‑1 (MQ=255)

                      aTCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGtt                                                                                                                     <  2:242608/141‑1 (MQ=255)

                      aTCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGtt                                                                                                                     >  2:575340/1‑141 (MQ=255)

                                 tCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCTATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGTCGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGc                                                                                                          <  2:38234/141‑1 (MQ=255)

                                 tCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGc                                                                                                          <  2:695018/141‑1 (MQ=255)

                                     ataACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCa                                                                                                      <  2:410151/141‑1 (MQ=255)

                                      taACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAg                                                                                                     <  2:716843/141‑1 (MQ=255)

                                       aaCTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGt                                                                                                    <  2:544257/141‑1 (MQ=255)

                                          ttATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGAGATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGtcat                                                                                                 >  1:715789/1‑141 (MQ=255)

                                               aGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTg                                                                                            >  2:359359/1‑141 (MQ=255)

                                               aGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTg                                                                                            >  2:765190/1‑141 (MQ=255)

                                               aGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTATCAGGCGTCAATTTAGACGCAGAGAGGAGGGGTA‑TAA‑GG‑T‑GATGCTTATTTGCGTTCACATCATAGGACCAGTCANCAGTg                                                                                            >  1:325587/1‑141 (MQ=255)

                                                gAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTgg                                                                                           >  2:491869/1‑141 (MQ=255)

                                                atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                          <  1:441038/127‑1 (MQ=255)

                                                 tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                         <  1:86719/128‑1 (MQ=255)

                                                 tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                         >  2:134697/14‑141 (MQ=255)

                                                 tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                         >  1:766917/14‑141 (MQ=255)

                                                  cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTACTCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                        <  1:220643/129‑1 (MQ=255)

                                                  cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                        >  2:321752/13‑141 (MQ=255)

                                                  aGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                         >  1:25598/1‑141 (MQ=255)

                                                   ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                       <  2:298877/130‑1 (MQ=255)

                                                   ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                       <  1:344186/130‑1 (MQ=255)

                                                     aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                     <  1:708545/132‑1 (MQ=255)

                                                     aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACTT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                     <  2:461037/132‑1 (MQ=255)

                                                       gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                   >  1:380518/8‑139 (MQ=255)

                                                       gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                   >  2:446439/8‑139 (MQ=255)

                                                        tGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCa                                                                                   >  2:596881/1‑141 (MQ=255)

                                                         gACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAt                                                                                  >  2:725956/1‑141 (MQ=255)

                                                         agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                                                                                 >  1:172496/6‑137 (MQ=255)

                                                          aCTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                                                                 <  2:163347/141‑1 (MQ=255)

                                                           cTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTg                                                                                >  2:743107/1‑141 (MQ=255)

                                                           cTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTg                                                                                <  2:369685/141‑1 (MQ=255)

                                                           atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                                                                               <  2:240950/138‑7 (MQ=255)

                                                           atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                                                                               <  1:435226/138‑7 (MQ=255)

                                                             gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCCGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCg                                                                              <  1:509642/141‑1 (MQ=255)

                                                             atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACTT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                             >  1:66944/2‑141 (MQ=255)

                                                              tgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGt                                                                             >  1:465381/1‑141 (MQ=255)

                                                               gCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtt                                                                            >  2:767435/1‑141 (MQ=255)

                                                                    tataTTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttttt                                                                       <  1:328835/141‑1 (MQ=255)

                                                                       atTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCtt                                                                    >  2:691718/1‑141 (MQ=255)

                                                                         ttGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAt                                                                  <  2:445190/141‑1 (MQ=255)

                                                                          tGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAtt                                                                 >  1:659158/1‑141 (MQ=255)

                                                                          cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACTT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                >  1:660933/5‑141 (MQ=255)

                                                                            cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGGAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                              >  1:534743/3‑141 (MQ=255)

                                                                            cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCTGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                              <  2:511793/139‑1 (MQ=255)

                                                                             cGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGTCGCAGAGAGGATGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGGTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGc                                                              <  2:69348/141‑1 (MQ=255)

                                                                             agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                             <  2:307427/140‑1 (MQ=255)

                                                                              gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                            <  1:643117/141‑2 (MQ=255)

                                                                               atgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTa                                                            <  2:719655/141‑1 (MQ=255)

                                                                                tgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTaa                                                           <  1:65302/141‑1 (MQ=255)

                                                                                 ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                         <  2:550474/139‑1 (MQ=255)

                                                                                  atatAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCATTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGt                                                         <  2:783048/141‑1 (MQ=255)

                                                                                  atatAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGt                                                         >  1:5472/1‑141 (MQ=255)

                                                                                   tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                       >  2:630932/1‑140 (MQ=255)

                                                                                    ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                      <  1:786287/141‑3 (MQ=255)

                                                                                    ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACTT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                      >  1:572266/1‑139 (MQ=255)

                                                                                      aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                                    <  1:712009/141‑1 (MQ=255)

                                                                                      aataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGAc                                                     >  2:36817/1‑141 (MQ=255)

                                                                                       ataatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                   >  2:25913/5‑141 (MQ=255)

                                                                                       ataatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CT‑TGGCTAGTCATGTGCGTTCACAGCATAGCACCAGTCATCAGTGGCTGGGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                   >  1:589508/5‑141 (MQ=255)

                                                                                       ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                   <  2:544784/141‑1 (MQ=255)

                                                                                       ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                   <  1:38084/141‑1 (MQ=255)

                                                                                       ataataGAGTCGCCTATCTCTCAGGCGGCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACTT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTTAACGc                                                   <  2:781962/141‑1 (MQ=255)

                                                                                       ataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACg                                                    >  1:246118/1‑141 (MQ=255)

                                                                                            agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTTT‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                              >  1:705478/1‑138 (MQ=255)

                                                                                              agTCGCCTATCTCTCATGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                            <  1:688335/141‑6 (MQ=255)

                                                                                              agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                            <  1:688326/141‑6 (MQ=255)

                                                                                                 cGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaaaa                                          >  2:332975/1‑141 (MQ=255)

                                                                                                  gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                        <  1:125747/141‑10 (MQ=255)

                                                                                                  gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                        <  1:111856/141‑10 (MQ=255)

                                                                                                  gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑TT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                        >  1:45923/1‑132 (MQ=255)

                                                                                                  gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACTT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                        >  2:200463/1‑132 (MQ=255)

                                                                                                    cTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAg                                       >  1:107906/1‑141 (MQ=255)

                                                                                                    cTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAg                                       >  1:590619/1‑141 (MQ=255)

                                                                                                     tATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAgg                                      <  1:643035/141‑1 (MQ=255)

                                                                                                         tctcAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGAc                                  >  2:386253/1‑141 (MQ=255)

                                                                                                            gcgcttacAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCCCCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACata                               <  1:196719/134‑1 (MQ=255)

                                                                                                              ggCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACAtata                             >  1:510608/1‑141 (MQ=255)

                                                                                                                  tCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCg                         >  2:582771/1‑141 (MQ=255)

                                                                                                                  tCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCg                         >  1:122423/1‑141 (MQ=255)

                                                                                                                   cAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTTGCTAAGTAGACGCAACACAAAATAGTTGACATATAGCCGc                        >  2:43261/1‑141 (MQ=255)

                                                                                                                   cAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGACGc                        >  2:43330/1‑141 (MQ=255)

                                                                                                                    aaTTTAGACGCAGAGAGGAGGTGTA‑TCA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACACAATAGGTGACATATAGCCGcc                       >  2:40916/1‑140 (MQ=255)

                                                                                                                      tttAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCAcc                     <  2:784410/141‑1 (MQ=255)

                                                                                                                              cAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaaaaa             <  1:191975/141‑1 (MQ=255)

                                                                                                                                    ggaggTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCt       >  2:212298/1‑141 (MQ=255)

                                                                                                                                       ggTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGACCTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCAc    >  1:410912/1‑141 (MQ=255)

                                                                                                                                         tgtA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTGGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACtc  <  2:240276/141‑2 (MQ=255)

TTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑GG‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTA  >  CP000730/1929722‑1929999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`