New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 2665976 =71 (1.090)3 (0.050) 3/238 NT 5.0% coding (476/900 nt) sdhA2 L‑serine ammonia‑lyase alpha subunit
?CP000730 2666008 = 49 (0.810)coding (444/900 nt) sdhA2 L‑serine ammonia‑lyase alpha subunit
Rejected: Frequency below/above cutoff threshold.

TTATTTGTGCAACGCCAACAGCTGGTTCCTCGGGTACCATTCCCGGTGCACTTTTTAAATTAGAAAAAACACATGATTTAACAGAAGAGCAAATGATTGATTTCTTATTCACTTCAGCATTGTTTGGGCGTGTCGTAGCAAACAATGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/2666123‑2665976
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑caaacaatgcTGAAGTGAATAAGAAATCAATCATTTGCTCTTCTGTTAAATCATGTGTTTTTTCTAATTTAAAAAGTGCACCGGGAATGGTACCCGAGGAACCAGCTGTTGGCGTTGCACAAATAATACCCATCGCAGCATTGACTTCAT  >  CP000730/2666008‑2666147
                                                                                                                                                                                                                                                                                                
TTATTTGTGCAACGCCAACAGCTGGTTCCTCGGGTACCATTCCCGGTGCACTTTTTAAATTAGAAAAAACACATGATTTAACAGAAGAGCAAATGATTGATTTCTTATTCACTTCAGCATTGTTTGGGCGTGTCGTAGCAA                                                                                                                                                     <  2:278862/141‑1
TTATTTGTGCAACGACACGGCCTGGTTCCGCGGGTACCATTCCCGGTGCACTTTTTAAATTAGAAAAAACACATGATTTCACAGCAGAGCAAATGATTGATGTCTGATTCACGTCAGCATGGTTTGGGCGTGTCGTAGCAA                                                                                                                                                     >  2:786120/1‑141
    TTGTGCAACGCCAACAGCTGGTTCCTCGGGTACCATTCCCGGTGCACTTTTTAAATTAGAAAAAACACATGATTTAACAGAAGAGCAAATGATTGATTTCTTATTCACTTCAGCATTGTTTGGGCGTGTCGTAGCAAACAA                                                                                                                                                 >  1:57265/1‑141
     TGTGCAACGCCAACAGCTGGTTCCTCGGGTACCATTCCCGGTGCACTTTTTAAATTAGAAAAAACACATGATTTAACAGAAGAGCAAATGATTGATTTCTTATTCACTTCAGCATTGTTTGGGCGTGTCGTAGCAAACAAT                                                                                                                                                >  1:713071/1‑141
      GTGCAACGCCAACAGCTGGTTCCTCGGGTACCATTCCCGGTGCACTTTTTAAATTAGAAAAAACACATGATTTAACAGAAGAGCAAATGATTGATTTCTTATTCACTTCAGCATTGTTTGGGCGTGTCGTAGCAAACAATG                                                                                                                                               >  2:156422/1‑141
       TGCAACGCCAACAGCTGGTTCCTCGGGTACCATTCCCGGTGCACTTTTTAAATTAGAAAAAACACATGATTTAACAGAAGAGCAAATGATTGATTTCTTATTCACTTCAGCATTGTTTGGGCGTGTCGTAGCAAACAATGC                                                                                                                                              >  1:762835/1‑141
       TGCAACGCCAACAGCTGGTTCCTCGGGTACCATTCCCGGTGCACTTTTTAAATTAGAAAAAACACATGATTTAACAGAAGAGCAAATGATTGATTTCTTATTCACTTCAGCATTGTTTGGGCGTGTCGTAGCAAACAATGC                                                                                                                                              <  2:195563/141‑1
         taatGCCAACAGCTGGTTCCTCGGGTACCATTCCCGGTGCACTTTTTAAATTAGAGAAAACACATGATTTAACAGAAGAGCAAATGATTGATTTCTTATTCACTTCAGCATTGTTTGGGCGTGTCGTAGCAAACAATtcaa                                                                                                                                            >  2:461174/5‑137
                                    CCATTCCCGGTGCACTTTTTAAATTAGAAAAAACACATGATTTAACAGAAGAGCAAATGATTGATTTCTTATTCACTTCAGCATTGTTTGGGCGTGTCGTAGCAAACAATGCTGAAGTGAATAAGAAATCAATCATTTGCT                                                                                                                 >  1:680798/1‑141
                                                                                                TTGATTTCTTATTCACTTCAGCATTGTTTGGGCGTGTCGTAGCAAACAATGCTGAAGTGAATAAGAAATCAATCATTTGCTCTTCTGTTAAATCATGTGTATTTTCTAATTTAAAAATTGCACCGGGAATGGTCCCTGAGG                                                     >  2:142865/1‑141
                                                                                                                         GTTTGGGCGTGTCGTAGCAAACAATGCTGAAGTGAATAAGAAATCAATCATTTGCTCTTCTGTTAAATCATGTGTTTTTTCTAATTTAAAAAGTGCACCGGGAATGGTACCCGAGGAACCAGCTGTTGGCGTTGCACAAAT                            >  2:422808/1‑141
                                                                                                                                              CAATGCTGAAGTGAATAAGAAATCAATCATTTGCTCTTCTGTTAAATCATGTGTTTTTTCTAATTTAAAAAGTGCACCGGGAATGGTACCCGAGGAACCAGCTGTTGGCGTTGCACAAATAATACCCATCGCAGCATTGAC       <  1:417960/141‑1
                                                                                                                                                 TGCTGAAGTGAATAAGAAATCAATCATTTGCTCTTCTGTTAAATCATGTGTTTTTTCTAATTTAAAAAGTGCACCGGGAATGGTACCCGAGGAACCAGCTGTTGGCGTTGCACAAATAATACCCATCGCAGCATTGACTTC    >  2:229345/1‑141
                                                                                                                                                  GCTGAAGTGAATAAGAAATCAATCATTTGCTCTTCTGTTAAATCATGTGTTTTTTCTAATTTAAAAAGTGCACCGGGAATGGTACCCGAGGAACCAGCTGTTGGCGTTGCACAAATAATACCCATCGCAGCATTGACTTCA   <  2:17413/141‑1
                                                                                                                                                   CTGAAGTGAATAAGAAATCAATCATTTGCTCTTCTGTTAAATCATGTGTTTTTTCTAATTTAAAAAGTGCACCGGGAATGGTACCCGAGGAACCAGCTGTTGGCGTTGCACAAATAATACCCATCGCAGCATTGACTTCAT  <  2:566568/141‑1
                                                                                                                                                   CTGAAGTGAATAAGAAATCAATCATTTGCTCTTCTGTTAAATCATGTGTTTTTTCTAATTTAAAAAGTGCACCGGGAATGGTACCCGAGGAACCAGCTGTTGGCGTTGCACAAATAATACCCATCGCAGCATTGACTTCAT  <  1:291494/141‑1
                                                                                                                                                                                                                                                                                                
TTATTTGTGCAACGCCAACAGCTGGTTCCTCGGGTACCATTCCCGGTGCACTTTTTAAATTAGAAAAAACACATGATTTAACAGAAGAGCAAATGATTGATTTCTTATTCACTTCAGCATTGTTTGGGCGTGTCGTAGCAAACAATGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/2666123‑2665976
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑caaacaatgcTGAAGTGAATAAGAAATCAATCATTTGCTCTTCTGTTAAATCATGTGTTTTTTCTAATTTAAAAAGTGCACCGGGAATGGTACCCGAGGAACCAGCTGTTGGCGTTGCACAAATAATACCCATCGCAGCATTGACTTCAT  >  CP000730/2666008‑2666147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.