Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,119,307 C→T 5.3% T313T (ACG→ACA)  USA300HOU_1992 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,119,3070CT 5.3% 222.9 / 2.7 75T313T (ACG→ACA) USA300HOU_1992hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (42/29);  new base T (2/2);  total (44/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.36e-01

TACATACATTGTCATCAAGATATTCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATT  >  CP000730/2119168‑2119447
                                                                                                                                            |                                                                                                                                            
tacatacaTTGTCATCAAGATATTCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCg                                                                                                                                             >  2:702530/1‑141 (MQ=255)
 acatacatTGTCATCAAGATATTCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGt                                                                                                                                            <  1:348968/141‑1 (MQ=255)
  catacatTGTCATCAAGATATTCAAA‑TTTTTTTTTTTTTTCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGtt                                                                                                                                           >  2:628749/1‑141 (MQ=255)
          gTCATCAAGATATTCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGGTTGTTTTGCTTTAATCTCGTTTTAAGttt                                                                                                                                   >  2:115775/1‑141 (MQ=255)
          gTCATCAAGATATTCAAA‑TTTTTGATGTTTAGCTTTTTCTAAAGCACTTTTGAATCCTTGATGATTTTCTAATAATGGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGtt                                                                                                                                   <  1:204383/141‑1 (MQ=255)
            catcaAGTTATTCAAA‑TTTTTGCTTTTTAGCTTTTTCTAAATCACTTTTTATGCAATTATGATTTGTTAATAATTGAAGAGATCTATTTGTTTCATGCGTGAGTAGGTTGTTGTTTTGCTTTATTCTCGTTTTAATGtttt                                                                                                                                 >  1:273232/1‑140 (MQ=255)
             atcaAGATATTCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGc                                                                                                                                <  2:76310/141‑1 (MQ=255)
             atcaAGATATTCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGc                                                                                                                                >  2:626366/1‑141 (MQ=255)
                aaGATATTCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTAc                                                                                                                             >  2:170651/1‑141 (MQ=255)
                  gATATTCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAATGTTTGCTACCg                                                                                                                           >  1:741488/1‑141 (MQ=255)
                      ttCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTcc                                                                                                                       <  2:721114/141‑1 (MQ=255)
                       tCAAATTTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTcc                                                                                                                       <  1:183332/141‑1 (MQ=255)
                       tCAAA‑TTTTTGATTTTTAGCTTTTTCTATTTCACTTTTTAATCCTTTATGATTTTCTAATAATTGTTTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTATGTTTTGCTACCGTTcct                                                                                                                      >  2:654268/1‑140 (MQ=255)
                       tCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTTTTTTTTTTTTTTTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTcct                                                                                                                      >  2:654329/1‑140 (MQ=255)
                       tCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTGGCTACCGTTCCa                                                                                                                      >  2:523999/1‑141 (MQ=255)
                          aa‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCAttt                                                                                                                   <  1:464517/141‑1 (MQ=255)
                             tttttGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTc                                                                                                                 >  1:258269/1‑141 (MQ=255)
                                 tGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACg                                                                                                             >  1:106879/1‑141 (MQ=255)
                                  tttttttttttttttCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTTTTTTTTTTTTTGCTTTAATCTCGTTTTAAGGTTTGCTTCCGTTCCATTTTCATCgt                                                                                                            >  2:146001/11‑141 (MQ=255)
                                  gATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACgt                                                                                                            >  2:161667/1‑141 (MQ=255)
                                  gATTGTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACgt                                                                                                            >  2:630094/1‑141 (MQ=255)
                                   aTTTTTAGCTTTTTCTAAATCACTTTTTTTTTTTTTTTTTTTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTCATCTCGTTTTATTTTTTGCTACCGTTCCTTTTTCAACgtt                                                                                                           >  2:653569/1‑140 (MQ=255)
                                   aTTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGAGATTTGTTGTTTGTTTTGCATTAATTTCGTTTTACGGTTTGCTACCGTTCCTTTTTCAACgtg                                                                                                           >  1:117976/1‑141 (MQ=255)
                                    tttttAGCTTTTTTTATTTTTTTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATTTCGTTTTATTGTTTTTTACTGTTCTTTTTTTAACgtgt                                                                                                          >  2:724076/1‑141 (MQ=255)
                                    tttttAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACgtgt                                                                                                          <  1:759624/141‑1 (MQ=255)
                                    tttttAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCGATTTTCTTCATGTGTAATTTGTTGTTTGTTTTGCTTTAATCTTGTTTTAAGATTCGCAACCGTTCCATTTTCAACgtgt                                                                                                          >  2:70273/1‑141 (MQ=255)
                                    tttttAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCGATTTTCTTCATGTGTAATTTGTTGTTTGTTTTGCTTTAATCTTGTTTTAAGATTCGCAACCGTTCCATTTTCAACgtgt                                                                                                          >  2:459053/1‑141 (MQ=255)
                                      tttAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTaa                                                                                                        >  1:166453/1‑141 (MQ=255)
                                       ttAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAAt                                                                                                       <  2:166453/141‑1 (MQ=255)
                                          gCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCa                                                                                                    >  1:276289/1‑141 (MQ=255)
                                             ttttCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCAttt                                                                                                 >  1:718105/1‑141 (MQ=255)
                                             tttcttaaaaacttttttAATCC‑TTATGATTTTTTAATAATTGTTTACGGCTTTTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTTAGGTTTGCTACCGTTTCCTTTTCAACATGTTATTCAtttt                                                                                                >  2:482242/14‑140 (MQ=255)
                                                       cACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCTTTTGTTAAAGCAt                                                                                       >  1:269279/1‑141 (MQ=255)
                                                       cACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCAt                                                                                       >  2:606813/1‑141 (MQ=255)
                                                        aCTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATg                                                                                      <  1:550011/141‑1 (MQ=255)
                                                        aCTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATg                                                                                      <  2:441631/141‑1 (MQ=255)
                                                        aCTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATg                                                                                      <  1:244284/141‑1 (MQ=255)
                                                            tttAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGAtt                                                                                  <  2:389852/141‑1 (MQ=255)
                                                             ttAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGAttt                                                                                 <  2:205885/141‑1 (MQ=255)
                                                                  ccTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTtc                                                                            <  1:418038/141‑1 (MQ=255)
                                                                  ccTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTtc                                                                            >  1:463510/1‑141 (MQ=255)
                                                                   cTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTtct                                                                           >  2:79839/1‑141 (MQ=255)
                                                                    tttATGTTTTTCTAATAATTGATTACGTATATTTTCTTCATGCGCGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTtctc                                                                          >  1:446845/1‑141 (MQ=255)
                                                                          aTTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGTTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAGGCATGGATTTTGTTCTCATTACt                                                                    >  2:352544/1‑141 (MQ=255)
                                                                            tttCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTg                                                                  <  2:537539/141‑1 (MQ=255)
                                                                             ttCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTgc                                                                 <  1:724076/141‑1 (MQ=255)
                                                                              tCTAATAATTTATTACGTTTGTTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGCTTGCTGTCGTACCATTTTCATCTTGTAAGTCATTTGGTAAAGCAGGGATTTTGGTCTCGTTAcaggcg                                                                <  2:747008/141‑6 (MQ=255)
                                                                                taataaTTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCt                                                              >  2:472014/1‑141 (MQ=255)
                                                                                  ataatTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGt                                                            >  1:321922/1‑141 (MQ=255)
                                                                                  ataatTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGt                                                            <  1:405180/141‑1 (MQ=255)
                                                                                  ataatTGATTACGTCTATTTTCTTCATGCGGGATTTGTTGTTTGTTTTGCTTTACTCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGTATTTTGTTCTCATTACTTGCGCTGt                                                            >  2:293512/1‑141 (MQ=255)
                                                                                    aatTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCAATTTTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTTTTa                                                          >  1:331759/1‑141 (MQ=255)
                                                                                      ttGATTACGTCTATTCTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTAtt                                                        <  1:653569/141‑1 (MQ=255)
                                                                                        gATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGt                                                      <  2:213997/141‑1 (MQ=255)
                                                                                              gTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTa                                                <  2:713647/141‑1 (MQ=255)
                                                                                               tCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTAt                                               <  2:515018/141‑1 (MQ=255)
                                                                                                  aTTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCg                                            >  1:104407/1‑141 (MQ=255)
                                                                                                      tcttcATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTc                                        <  2:29972/141‑1 (MQ=255)
                                                                                                          cATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAAtt                                    >  2:338334/1‑141 (MQ=255)
                                                                                                              cGTGGTTTGTTGTTTGTTTTGCTTTGATCTCGTTTTGAGGTTTGCTACCGTTCCTTTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTGTTGTCTTCTATGCGTTTCTATCCTGa                                <  2:110429/141‑1 (MQ=255)
                                                                                                                  aTTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGt                            >  2:98409/1‑141 (MQ=255)
                                                                                                                   tttGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGtt                           >  1:496625/1‑141 (MQ=255)
                                                                                                                     tgttgtTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTa                         <  1:1760/141‑1 (MQ=255)
                                                                                                                     tgttgtTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTa                         >  1:44687/1‑141 (MQ=255)
                                                                                                                       ttgtttgtTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATc                       <  2:113289/141‑1 (MQ=255)
                                                                                                                        tgtttgttTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCa                      <  1:626366/141‑1 (MQ=255)
                                                                                                                         gtttgtttTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGAGTGTTTATCAg                     <  2:223022/141‑1 (MQ=255)
                                                                                                                         gtttgtttTGCTTTAACCTCGTTCTAAGTATTGCTACCTTTCCAGTTTCATCGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAg                     <  1:619943/141‑1 (MQ=255)
                                                                                                                           ttgtttTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCt                   >  2:522790/1‑141 (MQ=255)
                                                                                                                               tttGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAAtt               >  2:286230/1‑141 (MQ=255)
                                                                                                                                 tGCTTTAATCTTGTTTTAAGATTCGCAACCGTTCCATTTTCAACGTGTAGCTCATTTGTTAAAGCATGTATTTTGTTCTCATTACTGGCGCTATTATTAGCTTCTATGCGCTTCAATTCTGATTGTTTATCAGCTAATTgg             <  1:459053/141‑2 (MQ=255)
                                                                                                                                 tGCTTTAATCTTGTTTTAAGATTCGCAACCGTTCCATTTTCAACGTGTAGCTCATTTGTTAAAGCATGTATTTTGTTCTCATTACTGGCGCTATTATTAGCTTCTATGCGCTTCAATTCTGATTGTTTATCAGCTAATTgg             <  1:70273/141‑2 (MQ=255)
                                                                                                                                       aaTCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGt       >  2:100914/1‑141 (MQ=255)
                                                                                                                                        aTCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTa      >  1:615641/1‑141 (MQ=255)
                                                                                                                                        aTCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTa      >  1:60961/1‑141 (MQ=255)
                                                                                                                                            cGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAaatt  <  1:640333/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TACATACATTGTCATCAAGATATTCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATT  >  CP000730/2119168‑2119447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑