Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,119,316 G→A 5.3% N310N (AAC→AAT)  USA300HOU_1992 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,119,3160GA 5.3% 226.2 / 2.8 78N310N (AAC→AAT) USA300HOU_1992hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (39/32);  new base A (2/2);  total (43/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01

GTCATCAAGATATTCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATTTCTTC  >  CP000730/2119178‑2119456
                                                                                                                                           |                                                                                                                                            
gTCATCAAGATATTCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGGTTGTTTTGCTTTAATCTCGTTTTAAGttt                                                                                                                                            >  2:115775/1‑141 (MQ=255)
gTCATCAAGATATTCAAA‑TTTTTGATGTTTAGCTTTTTCTAAAGCACTTTTGAATCCTTGATGATTTTCTAATAATGGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGtt                                                                                                                                            <  1:204383/141‑1 (MQ=255)
  catcaAGTTATTCAAA‑TTTTTGCTTTTTAGCTTTTTCTAAATCACTTTTTATGCAATTATGATTTGTTAATAATTGAAGAGATCTATTTGTTTCATGCGTGAGTAGGTTGTTGTTTTGCTTTATTCTCGTTTTAATGtttt                                                                                                                                          >  1:273232/1‑140 (MQ=255)
   atcaAGATATTCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGc                                                                                                                                         >  2:626366/1‑141 (MQ=255)
   atcaAGATATTCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGc                                                                                                                                         <  2:76310/141‑1 (MQ=255)
      aaGATATTCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTAc                                                                                                                                      >  2:170651/1‑141 (MQ=255)
        gATATTCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAATGTTTGCTACCg                                                                                                                                    >  1:741488/1‑141 (MQ=255)
            ttCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTcc                                                                                                                                <  2:721114/141‑1 (MQ=255)
             tCAAATTTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTcc                                                                                                                                <  1:183332/141‑1 (MQ=255)
             tCAAA‑TTTTTGATTTTTAGCTTTTTCTATTTCACTTTTTAATCCTTTATGATTTTCTAATAATTGTTTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTATGTTTTGCTACCGTTcct                                                                                                                               >  2:654268/1‑140 (MQ=255)
             tCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTTTTTTTTTTTTTTTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTcct                                                                                                                               >  2:654329/1‑140 (MQ=255)
             tCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTGGCTACCGTTCCa                                                                                                                               >  2:523999/1‑141 (MQ=255)
                aa‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCAttt                                                                                                                            <  1:464517/141‑1 (MQ=255)
                   tttttGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTc                                                                                                                          >  1:258269/1‑141 (MQ=255)
                       tGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACg                                                                                                                      >  1:106879/1‑141 (MQ=255)
                        tttttttttttttttCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTTTTTTTTTTTTTGCTTTAATCTCGTTTTAAGGTTTGCTTCCGTTCCATTTTCATCgt                                                                                                                     >  2:146001/11‑141 (MQ=255)
                        gATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACgt                                                                                                                     >  2:161667/1‑141 (MQ=255)
                        gATTGTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACgt                                                                                                                     >  2:630094/1‑141 (MQ=255)
                         aTTTTTAGCTTTTTCTAAATCACTTTTTTTTTTTTTTTTTTTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTCATCTCGTTTTATTTTTTGCTACCGTTCCTTTTTCAACgtt                                                                                                                    >  2:653569/1‑140 (MQ=255)
                         aTTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGAGATTTGTTGTTTGTTTTGCATTAATTTCGTTTTACGGTTTGCTACCGTTCCTTTTTCAACgtg                                                                                                                    >  1:117976/1‑141 (MQ=255)
                          tttttAGCTTTTTTTATTTTTTTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATTTCGTTTTATTGTTTTTTACTGTTCTTTTTTTAACgtgt                                                                                                                   >  2:724076/1‑141 (MQ=255)
                          tttttAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACgtgt                                                                                                                   <  1:759624/141‑1 (MQ=255)
                          tttttAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCGATTTTCTTCATGTGTAATTTGTTGTTTGTTTTGCTTTAATCTTGTTTTAAGATTCGCAACCGTTCCATTTTCAACgtgt                                                                                                                   >  2:459053/1‑141 (MQ=255)
                          tttttAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCGATTTTCTTCATGTGTAATTTGTTGTTTGTTTTGCTTTAATCTTGTTTTAAGATTCGCAACCGTTCCATTTTCAACgtgt                                                                                                                   >  2:70273/1‑141 (MQ=255)
                            tttAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTaa                                                                                                                 >  1:166453/1‑141 (MQ=255)
                             ttAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAAt                                                                                                                <  2:166453/141‑1 (MQ=255)
                                gCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCa                                                                                                             >  1:276289/1‑141 (MQ=255)
                                   ttttCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCAttt                                                                                                          >  1:718105/1‑141 (MQ=255)
                                   tttcttaaaaacttttttAATCC‑TTATGATTTTTTAATAATTGTTTACGGCTTTTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTTAGGTTTGCTACCGTTTCCTTTTCAACATGTTATTCAtttt                                                                                                         >  2:482242/14‑140 (MQ=255)
                                             cACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCTTTTGTTAAAGCAt                                                                                                >  1:269279/1‑141 (MQ=255)
                                             cACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCAt                                                                                                >  2:606813/1‑141 (MQ=255)
                                              aCTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATg                                                                                               <  2:441631/141‑1 (MQ=255)
                                              aCTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATg                                                                                               <  1:244284/141‑1 (MQ=255)
                                              aCTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATg                                                                                               <  1:550011/141‑1 (MQ=255)
                                                  tttAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGAtt                                                                                           <  2:389852/141‑1 (MQ=255)
                                                   ttAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGAttt                                                                                          <  2:205885/141‑1 (MQ=255)
                                                        ccTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTtc                                                                                     >  1:463510/1‑141 (MQ=255)
                                                        ccTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTtc                                                                                     <  1:418038/141‑1 (MQ=255)
                                                         cTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTtct                                                                                    >  2:79839/1‑141 (MQ=255)
                                                          tttATGTTTTTCTAATAATTGATTACGTATATTTTCTTCATGCGCGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTtctc                                                                                   >  1:446845/1‑141 (MQ=255)
                                                                aTTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGTTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAGGCATGGATTTTGTTCTCATTACt                                                                             >  2:352544/1‑141 (MQ=255)
                                                                  tttCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTg                                                                           <  2:537539/141‑1 (MQ=255)
                                                                   ttCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTgc                                                                          <  1:724076/141‑1 (MQ=255)
                                                                    tCTAATAATTTATTACGTTTGTTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGCTTGCTGTCGTACCATTTTCATCTTGTAAGTCATTTGGTAAAGCAGGGATTTTGGTCTCGTTAcaggcg                                                                         <  2:747008/141‑6 (MQ=255)
                                                                      taataaTTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCt                                                                       >  2:472014/1‑141 (MQ=255)
                                                                        ataatTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGt                                                                     >  1:321922/1‑141 (MQ=255)
                                                                        ataatTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGt                                                                     <  1:405180/141‑1 (MQ=255)
                                                                        ataatTGATTACGTCTATTTTCTTCATGCGGGATTTGTTGTTTGTTTTGCTTTACTCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGTATTTTGTTCTCATTACTTGCGCTGt                                                                     >  2:293512/1‑141 (MQ=255)
                                                                          aatTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCAATTTTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTTTTa                                                                   >  1:331759/1‑141 (MQ=255)
                                                                            ttGATTACGTCTATTCTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTAtt                                                                 <  1:653569/141‑1 (MQ=255)
                                                                              gATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGt                                                               <  2:213997/141‑1 (MQ=255)
                                                                                    gTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTa                                                         <  2:713647/141‑1 (MQ=255)
                                                                                     tCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTAt                                                        <  2:515018/141‑1 (MQ=255)
                                                                                        aTTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCg                                                     >  1:104407/1‑141 (MQ=255)
                                                                                            tcttcATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTc                                                 <  2:29972/141‑1 (MQ=255)
                                                                                                cATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAAtt                                             >  2:338334/1‑141 (MQ=255)
                                                                                                    cGTGGTTTGTTGTTTGTTTTGCTTTGATCTCGTTTTGAGGTTTGCTACCGTTCCTTTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTGTTGTCTTCTATGCGTTTCTATCCTGa                                         <  2:110429/141‑1 (MQ=255)
                                                                                                        aTTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGt                                     >  2:98409/1‑141 (MQ=255)
                                                                                                         tttGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGtt                                    >  1:496625/1‑141 (MQ=255)
                                                                                                           tgttgtTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTa                                  <  1:1760/141‑1 (MQ=255)
                                                                                                           tgttgtTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTa                                  >  1:44687/1‑141 (MQ=255)
                                                                                                             ttgtttgtTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATc                                <  2:113289/141‑1 (MQ=255)
                                                                                                              tgtttgttTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCa                               <  1:626366/141‑1 (MQ=255)
                                                                                                               gtttgtttTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGAGTGTTTATCAg                              <  2:223022/141‑1 (MQ=255)
                                                                                                               gtttgtttTGCTTTAACCTCGTTCTAAGTATTGCTACCTTTCCAGTTTCATCGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAg                              <  1:619943/141‑1 (MQ=255)
                                                                                                                 ttgtttTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCt                            >  2:522790/1‑141 (MQ=255)
                                                                                                                     tttGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAAtt                        >  2:286230/1‑141 (MQ=255)
                                                                                                                       tGCTTTAATCTTGTTTTAAGATTCGCAACCGTTCCATTTTCAACGTGTAGCTCATTTGTTAAAGCATGTATTTTGTTCTCATTACTGGCGCTATTATTAGCTTCTATGCGCTTCAATTCTGATTGTTTATCAGCTAATTgg                      <  1:70273/141‑2 (MQ=255)
                                                                                                                       tGCTTTAATCTTGTTTTAAGATTCGCAACCGTTCCATTTTCAACGTGTAGCTCATTTGTTAAAGCATGTATTTTGTTCTCATTACTGGCGCTATTATTAGCTTCTATGCGCTTCAATTCTGATTGTTTATCAGCTAATTgg                      <  1:459053/141‑2 (MQ=255)
                                                                                                                             aaTCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGt                >  2:100914/1‑141 (MQ=255)
                                                                                                                              aTCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTa               >  1:60961/1‑141 (MQ=255)
                                                                                                                              aTCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTa               >  1:615641/1‑141 (MQ=255)
                                                                                                                                  cGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAaatt           <  1:640333/141‑1 (MQ=255)
                                                                                                                                   gTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAaatta          >  1:401886/1‑141 (MQ=255)
                                                                                                                                    ttttAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGCTTGTTTATCAGCTAATTGATTACGTAaattaa         >  2:598180/1‑141 (MQ=255)
                                                                                                                                       tAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAAttt      <  1:294706/141‑1 (MQ=255)
                                                                                                                                       tAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAAttt      <  2:437409/141‑1 (MQ=255)
                                                                                                                                       tAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAAttt      <  2:411533/141‑1 (MQ=255)
                                                                                                                                        aaGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATttc     <  1:151308/141‑1 (MQ=255)
                                                                                                                                          ggTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATttctt   >  1:759783/1‑141 (MQ=255)
                                                                                                                                           gTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATttcttc  <  2:258510/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
GTCATCAAGATATTCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATTTCTTC  >  CP000730/2119178‑2119456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑