Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,119,319 T→C 5.2% A309A (GCA→GCG)  USA300HOU_1992 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,119,3190TC 5.2% 240.6 / 3.1 78A309A (GCA→GCG) USA300HOU_1992hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (40/33);  new base C (2/2);  total (43/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01

CATCAAGATATTCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATTTCTTCCT  >  CP000730/2119180‑2119458
                                                                                                                                            |                                                                                                                                           
catcaAGTTATTCAAA‑TTTTTGCTTTTTAGCTTTTTCTAAATCACTTTTTATGCAATTATGATTTGTTAATAATTGAAGAGATCTATTTGTTTCATGCGTGAGTAGGTTGTTGTTTTGCTTTATTCTCGTTTTAATGtttt                                                                                                                                            >  1:273232/1‑140 (MQ=255)
 atcaAGATATTCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGc                                                                                                                                           >  2:626366/1‑141 (MQ=255)
 atcaAGATATTCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGc                                                                                                                                           <  2:76310/141‑1 (MQ=255)
    aaGATATTCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTAc                                                                                                                                        >  2:170651/1‑141 (MQ=255)
      gATATTCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAATGTTTGCTACCg                                                                                                                                      >  1:741488/1‑141 (MQ=255)
          ttCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTcc                                                                                                                                  <  2:721114/141‑1 (MQ=255)
           tCAAATTTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTcc                                                                                                                                  <  1:183332/141‑1 (MQ=255)
           tCAAA‑TTTTTGATTTTTAGCTTTTTCTATTTCACTTTTTAATCCTTTATGATTTTCTAATAATTGTTTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTATGTTTTGCTACCGTTcct                                                                                                                                 >  2:654268/1‑140 (MQ=255)
           tCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTTTTTTTTTTTTTTTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTcct                                                                                                                                 >  2:654329/1‑140 (MQ=255)
           tCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTGGCTACCGTTCCa                                                                                                                                 >  2:523999/1‑141 (MQ=255)
              aa‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCAttt                                                                                                                              <  1:464517/141‑1 (MQ=255)
                 tttttGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTc                                                                                                                            >  1:258269/1‑141 (MQ=255)
                     tGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACg                                                                                                                        >  1:106879/1‑141 (MQ=255)
                      tttttttttttttttCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTTTTTTTTTTTTTGCTTTAATCTCGTTTTAAGGTTTGCTTCCGTTCCATTTTCATCgt                                                                                                                       >  2:146001/11‑141 (MQ=255)
                      gATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACgt                                                                                                                       >  2:161667/1‑141 (MQ=255)
                      gATTGTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACgt                                                                                                                       >  2:630094/1‑141 (MQ=255)
                       aTTTTTAGCTTTTTCTAAATCACTTTTTTTTTTTTTTTTTTTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTCATCTCGTTTTATTTTTTGCTACCGTTCCTTTTTCAACgtt                                                                                                                      >  2:653569/1‑140 (MQ=255)
                       aTTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGAGATTTGTTGTTTGTTTTGCATTAATTTCGTTTTACGGTTTGCTACCGTTCCTTTTTCAACgtg                                                                                                                      >  1:117976/1‑141 (MQ=255)
                        tttttAGCTTTTTTTATTTTTTTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATTTCGTTTTATTGTTTTTTACTGTTCTTTTTTTAACgtgt                                                                                                                     >  2:724076/1‑141 (MQ=255)
                        tttttAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACgtgt                                                                                                                     <  1:759624/141‑1 (MQ=255)
                        tttttAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCGATTTTCTTCATGTGTAATTTGTTGTTTGTTTTGCTTTAATCTTGTTTTAAGATTCGCAACCGTTCCATTTTCAACgtgt                                                                                                                     >  2:70273/1‑141 (MQ=255)
                        tttttAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCGATTTTCTTCATGTGTAATTTGTTGTTTGTTTTGCTTTAATCTTGTTTTAAGATTCGCAACCGTTCCATTTTCAACgtgt                                                                                                                     >  2:459053/1‑141 (MQ=255)
                          tttAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTaa                                                                                                                   >  1:166453/1‑141 (MQ=255)
                           ttAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAAt                                                                                                                  <  2:166453/141‑1 (MQ=255)
                              gCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCa                                                                                                               >  1:276289/1‑141 (MQ=255)
                                 ttttCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCAttt                                                                                                            >  1:718105/1‑141 (MQ=255)
                                 tttcttaaaaacttttttAATCC‑TTATGATTTTTTAATAATTGTTTACGGCTTTTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTTAGGTTTGCTACCGTTTCCTTTTCAACATGTTATTCAtttt                                                                                                           >  2:482242/14‑140 (MQ=255)
                                           cACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCTTTTGTTAAAGCAt                                                                                                  >  1:269279/1‑141 (MQ=255)
                                           cACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCAt                                                                                                  >  2:606813/1‑141 (MQ=255)
                                            aCTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATg                                                                                                 <  1:550011/141‑1 (MQ=255)
                                            aCTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATg                                                                                                 <  1:244284/141‑1 (MQ=255)
                                            aCTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATg                                                                                                 <  2:441631/141‑1 (MQ=255)
                                                tttAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGAtt                                                                                             <  2:389852/141‑1 (MQ=255)
                                                 ttAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGAttt                                                                                            <  2:205885/141‑1 (MQ=255)
                                                      ccTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTtc                                                                                       >  1:463510/1‑141 (MQ=255)
                                                      ccTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTtc                                                                                       <  1:418038/141‑1 (MQ=255)
                                                       cTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTtct                                                                                      >  2:79839/1‑141 (MQ=255)
                                                        tttATGTTTTTCTAATAATTGATTACGTATATTTTCTTCATGCGCGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTtctc                                                                                     >  1:446845/1‑141 (MQ=255)
                                                              aTTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGTTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAGGCATGGATTTTGTTCTCATTACt                                                                               >  2:352544/1‑141 (MQ=255)
                                                                tttCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTg                                                                             <  2:537539/141‑1 (MQ=255)
                                                                 ttCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTgc                                                                            <  1:724076/141‑1 (MQ=255)
                                                                  tCTAATAATTTATTACGTTTGTTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGCTTGCTGTCGTACCATTTTCATCTTGTAAGTCATTTGGTAAAGCAGGGATTTTGGTCTCGTTAcaggcg                                                                           <  2:747008/141‑6 (MQ=255)
                                                                    taataaTTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCt                                                                         >  2:472014/1‑141 (MQ=255)
                                                                      ataatTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGt                                                                       >  1:321922/1‑141 (MQ=255)
                                                                      ataatTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGt                                                                       <  1:405180/141‑1 (MQ=255)
                                                                      ataatTGATTACGTCTATTTTCTTCATGCGGGATTTGTTGTTTGTTTTGCTTTACTCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGTATTTTGTTCTCATTACTTGCGCTGt                                                                       >  2:293512/1‑141 (MQ=255)
                                                                        aatTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCAATTTTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTTTTa                                                                     >  1:331759/1‑141 (MQ=255)
                                                                          ttGATTACGTCTATTCTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTAtt                                                                   <  1:653569/141‑1 (MQ=255)
                                                                            gATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGt                                                                 <  2:213997/141‑1 (MQ=255)
                                                                                  gTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTa                                                           <  2:713647/141‑1 (MQ=255)
                                                                                   tCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTAt                                                          <  2:515018/141‑1 (MQ=255)
                                                                                      aTTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCg                                                       >  1:104407/1‑141 (MQ=255)
                                                                                          tcttcATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTc                                                   <  2:29972/141‑1 (MQ=255)
                                                                                              cATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAAtt                                               >  2:338334/1‑141 (MQ=255)
                                                                                                  cGTGGTTTGTTGTTTGTTTTGCTTTGATCTCGTTTTGAGGTTTGCTACCGTTCCTTTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTGTTGTCTTCTATGCGTTTCTATCCTGa                                           <  2:110429/141‑1 (MQ=255)
                                                                                                      aTTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGt                                       >  2:98409/1‑141 (MQ=255)
                                                                                                       tttGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGtt                                      >  1:496625/1‑141 (MQ=255)
                                                                                                         tgttgtTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTa                                    <  1:1760/141‑1 (MQ=255)
                                                                                                         tgttgtTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTa                                    >  1:44687/1‑141 (MQ=255)
                                                                                                           ttgtttgtTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATc                                  <  2:113289/141‑1 (MQ=255)
                                                                                                            tgtttgttTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCa                                 <  1:626366/141‑1 (MQ=255)
                                                                                                             gtttgtttTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGAGTGTTTATCAg                                <  2:223022/141‑1 (MQ=255)
                                                                                                             gtttgtttTGCTTTAACCTCGTTCTAAGTATTGCTACCTTTCCAGTTTCATCGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAg                                <  1:619943/141‑1 (MQ=255)
                                                                                                               ttgtttTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCt                              >  2:522790/1‑141 (MQ=255)
                                                                                                                   tttGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAAtt                          >  2:286230/1‑141 (MQ=255)
                                                                                                                     tGCTTTAATCTTGTTTTAAGATTCGCAACCGTTCCATTTTCAACGTGTAGCTCATTTGTTAAAGCATGTATTTTGTTCTCATTACTGGCGCTATTATTAGCTTCTATGCGCTTCAATTCTGATTGTTTATCAGCTAATTgg                        <  1:70273/141‑2 (MQ=255)
                                                                                                                     tGCTTTAATCTTGTTTTAAGATTCGCAACCGTTCCATTTTCAACGTGTAGCTCATTTGTTAAAGCATGTATTTTGTTCTCATTACTGGCGCTATTATTAGCTTCTATGCGCTTCAATTCTGATTGTTTATCAGCTAATTgg                        <  1:459053/141‑2 (MQ=255)
                                                                                                                           aaTCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGt                  >  2:100914/1‑141 (MQ=255)
                                                                                                                            aTCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTa                 >  1:60961/1‑141 (MQ=255)
                                                                                                                            aTCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTa                 >  1:615641/1‑141 (MQ=255)
                                                                                                                                cGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAaatt             <  1:640333/141‑1 (MQ=255)
                                                                                                                                 gTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAaatta            >  1:401886/1‑141 (MQ=255)
                                                                                                                                  ttttAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGCTTGTTTATCAGCTAATTGATTACGTAaattaa           >  2:598180/1‑141 (MQ=255)
                                                                                                                                     tAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAAttt        <  2:437409/141‑1 (MQ=255)
                                                                                                                                     tAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAAttt        <  2:411533/141‑1 (MQ=255)
                                                                                                                                     tAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAAttt        <  1:294706/141‑1 (MQ=255)
                                                                                                                                      aaGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATttc       <  1:151308/141‑1 (MQ=255)
                                                                                                                                        ggTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATttctt     >  1:759783/1‑141 (MQ=255)
                                                                                                                                         gTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATttcttc    <  2:258510/141‑1 (MQ=255)
                                                                                                                                          tttGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATTTCTTcc   <  1:473554/141‑1 (MQ=255)
                                                                                                                                           ttGCTACCGTTCCATTTTCAACTTGTAATTCATTTGTTTAAGCATGGATTTTGTTCTCATTTCTTGTGCTGTTATTGTCTTCTTTGCGTTTCAATTCTGTTTGTTTTTCTGCTAATTGATTACGTAAATTAATTTCTTCCt  <  2:776797/141‑1 (MQ=255)
                                                                                                                                           ttGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAGATTAATTTCTTCCt  >  1:518188/1‑141 (MQ=255)
                                                                                                                                           ttGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATTTCTTCCt  <  1:304126/141‑1 (MQ=255)
                                                                                                                                           ttGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATTTCTTCCt  <  2:741488/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
CATCAAGATATTCAAA‑TTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGATTGTTTATCAGCTAATTGATTACGTAAATTAATTTCTTCCT  >  CP000730/2119180‑2119458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑