Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,513,975	C→T	5.7%	R54Q (CGA→CAA)	nreA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,513,975	0	C	T	5.7%	272.5 / 4.9	88	R54Q (CGA→CAA)	nreA	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (44/39);  new base T (3/2);  total (47/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01

TTCGCTCAATAAAATTGGATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTGAAATCTACGATCTTC  >  CP000730/2513836‑2514114                                                                                                                                            |                                                                                                                                            ttCGCTCAATAAAATTGGATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCg                                                                                                                                            <  1:525519/141‑1 (MQ=255)     cTCAATAAAATTGGATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGatta                                                                                                                                        >  2:411700/1‑141 (MQ=255)      tCAATAAAATTGGATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGattat                                                                                                                                       <  2:337789/141‑1 (MQ=255)        aaTAAAATTGGATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTAttt                                                                                                                                     >  1:15297/1‑141 (MQ=255)         aTAAAATTGGATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCGATTTAAATTGATTAtttt                                                                                                                                    <  1:510647/141‑1 (MQ=255)               ttGGATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTCTTAc                                                                                                                              >  2:437764/1‑141 (MQ=255)                 ggATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCt                                                                                                                            >  1:663570/1‑141 (MQ=255)                 ggATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCt                                                                                                                            >  2:88267/1‑141 (MQ=255)                   aTAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGa                                                                                                                          >  2:506490/1‑141 (MQ=255)                           tCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGt                                                                                                                  <  2:83642/141‑1 (MQ=255)                            cTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTg                                                                                                                 >  2:96438/1‑141 (MQ=255)                            cTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTg                                                                                                                 <  2:766028/141‑1 (MQ=255)                             ttttCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGc                                                                                                                <  1:753822/141‑1 (MQ=255)                             ttttCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGc                                                                                                                <  2:492174/141‑1 (MQ=255)                                   tcAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCAtttt                                                                                                          >  2:93751/1‑141 (MQ=255)                                   tcAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCAtttt                                                                                                          >  2:256024/1‑141 (MQ=255)                                     aGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTAt                                                                                                        >  1:527479/1‑141 (MQ=255)                                      ggCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTAtt                                                                                                       >  2:40407/1‑141 (MQ=255)                                       gCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTg                                                                                                      <  2:67515/141‑1 (MQ=255)                                       gCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTg                                                                                                      <  1:518443/141‑1 (MQ=255)                                         ccTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGga                                                                                                    <  1:244992/141‑1 (MQ=255)                                             aaGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAgatg                                                                                                >  1:558347/1‑141 (MQ=255)                                             aaGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAgatg                                                                                                <  1:362550/141‑1 (MQ=255)                                             aaGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAgatg                                                                                                <  1:128622/141‑1 (MQ=255)                                              aGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAgatga                                                                                               <  1:431258/141‑1 (MQ=255)                                              aGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAgatga                                                                                               >  1:295640/1‑141 (MQ=255)                                               gCTAAACCATCATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAGATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAgatgat                                                                                              <  2:480202/141‑1 (MQ=255)                                                 tAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATgg                                                                                            <  2:190699/141‑1 (MQ=255)                                                  aaaCCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACCTAGTGCCATTATATTGGCGatgatgtt                                                                                           >  2:463542/1‑139 (MQ=255)                                                      cAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGt                                                                                       <  1:135086/141‑1 (MQ=255)                                                      cAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGt                                                                                       <  1:135061/141‑1 (MQ=255)                                                      cAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGt                                                                                       <  2:477284/141‑1 (MQ=255)                                                         cATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAg                                                                                    >  1:436642/1‑141 (MQ=255)                                                            tGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGAtt                                                                                 >  1:292433/1‑141 (MQ=255)                                                            tGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGAtt                                                                                 >  2:40628/1‑141 (MQ=255)                                                            tGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGAtt                                                                                 >  2:351478/1‑141 (MQ=255)                                                             ggCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTc                                                                                >  2:156939/1‑141 (MQ=255)                                                                   tACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGaa                                                                          >  1:376100/1‑141 (MQ=255)                                                                    aCCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGaaa                                                                         >  2:58191/1‑141 (MQ=255)                                                                          cGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGAtt                                                                   <  2:77818/141‑1 (MQ=255)                                                                             tttCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGcc                                                                >  1:73025/1‑141 (MQ=255)                                                                              ttCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCg                                                               >  1:145479/1‑141 (MQ=255)                                                                                ccTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCa                                                             >  1:754737/1‑141 (MQ=255)                                                                                ccTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCa                                                             >  1:195475/1‑141 (MQ=255)                                                                                 cTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCaa                                                            >  1:311721/1‑141 (MQ=255)                                                                                  tGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCaaa                                                           <  2:811/141‑1 (MQ=255)                                                                                      ttCATAACAGTTCCAGCTAATCAACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGCTGATGGTTTGTTAGCTACATAGAAAGCGATTGCCGAAAAATCa                                                       >  2:209609/1‑141 (MQ=255)                                                                                        gcactatgTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGc                                                                                                                >  2:342508/8‑82 (MQ=255)                                                                                        gcactatgTTCCAGCTAATCCACGACCTTTGCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGc                                                                                                                <  1:342508/75‑1 (MQ=255)                                                                                           aaCAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAAcc                                                  >  1:722271/1‑141 (MQ=255)                                                                                            aCAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAAccc                                                 >  1:558090/1‑141 (MQ=255)                                                                                             cAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCct                                                <  1:319212/141‑1 (MQ=255)                                                                                             cAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCct                                                <  2:139074/141‑1 (MQ=255)                                                                                               gTTCCAGCTAATCCACGACCTTTCCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCctct                                              >  2:303370/1‑141 (MQ=255)                                                                                                 tCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCtcttc                                            >  2:132441/1‑141 (MQ=255)                                                                                                  ccAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCtcttct                                           >  2:329306/1‑141 (MQ=255)                                                                                                  ccAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCtcttct                                           <  1:443640/141‑1 (MQ=255)                                                                                                   cAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTcttctt                                          <  1:738987/141‑1 (MQ=255)                                                                                                   cAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAAGCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTcttctt                                          >  2:674965/1‑141 (MQ=255)                                                                                                    aGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAATCATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCCTAGAAAGCTATTGCCGCAAAATCATAACCCTCTTCttt                                         <  2:37189/141‑1 (MQ=255)                                                                                                       tAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCg                                      <  2:401484/141‑1 (MQ=255)                                                                                                         aTCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTa                                    >  1:347230/1‑141 (MQ=255)                                                                                                         aTCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAAACCTATTCTTTGCGTa                                    >  2:144792/1‑141 (MQ=255)                                                                                                           ccACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTAtt                                  <  2:463257/141‑1 (MQ=255)                                                                                                           ccACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTAtt                                  >  2:554616/1‑141 (MQ=255)                                                                                                            cACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTCGATTAATAGAAAGCGATTGCCGAAAAACCATAACCCTCTTCTTTGCGTAttt                                 >  1:662381/1‑141 (MQ=255)                                                                                                             aCGACCTTTTCTTAAAATAAGCAATTGAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTAtttt                                <  2:622431/141‑1 (MQ=255)                                                                                                              cGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTa                               <  2:745316/141‑1 (MQ=255)                                                                                                                  cTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTa                           <  1:40407/141‑1 (MQ=255)                                                                                                                   ttttCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTaa                          <  2:559369/141‑1 (MQ=255)                                                                                                                    tttCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAAt                         <  2:545187/141‑1 (MQ=255)                                                                                                                     ttCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGGTGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATg                        <  2:724158/141‑1 (MQ=255)                                                                                                                      tCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGt                       >  2:301824/1‑141 (MQ=255)                                                                                                                          aaaaTAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTctct                   <  1:40628/141‑1 (MQ=255)                                                                                                                           aaaTAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCtt                  <  1:114789/141‑1 (MQ=255)                                                                                                                           aaaTAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCtt                  >  2:646207/1‑141 (MQ=255)                                                                                                                            aataatCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTg                 >  2:46312/1‑141 (MQ=255)                                                                                                                             ataatCGATTTAAATTGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCAt                                                      <  2:585508/103‑1 (MQ=255)                                                                                                                             ataatCGATTTAAATTGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCAt                                                      >  1:585508/1‑103 (MQ=255)                                                                                                                              taatCGATTTAAATTGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTGCTTTGTGTATTTTATCTAATGTCTCTTGaa               >  1:240628/1‑141 (MQ=255)                                                                                                                                atCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTGAAAt             <  2:172617/141‑1 (MQ=255)                                                                                                                                  cgaTTTAAATTGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTGAAATCt           >  2:232409/3‑141 (MQ=255)                                                                                                                                  cAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTGAAATCt           <  1:761781/141‑1 (MQ=255)                                                                                                                                     tttAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTGAAATCTACg        >  1:460837/1‑141 (MQ=255)                                                                                                                                     tttAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTGAAATCTACg        <  2:15297/141‑1 (MQ=255)                                                                                                                                         aaTCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTGAAATCTACGATCt    <  1:302382/141‑1 (MQ=255)                                                                                                                                          aTCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTGAAATCTACGATCtt   >  1:389913/1‑141 (MQ=255)                                                                                                                                           tCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTGAAATCTACGATCTTc  >  1:672647/1‑141 (MQ=255)                                                                                                                                            |                                                                                                                                            TTCGCTCAATAAAATTGGATAGTCAATCTTTTCTTCAGGCCCTAAAGCTAAACCAACATTGGCTATTACCATACGCTTTCCTGTTTTCATAACAGTTCCAGCTAATCCACGACCTTTTCTTAAAATAATCAATTTAAATCGATTATTTTTATTACCTGAAACATAGTGCCATTTTATTGGAGATGATGGTTTGTTAGATTCATAGAAAGCGATTGCCGCAAAATCATAACCCTCTTCTTTGCGTATTTTATCTAATGTCTCTTGAAATCTACGATCTTC  >  CP000730/2513836‑2514114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑