Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,559,841 C→A 8.5% intergenic (+35/+88) bcr → / ← USA300HOU_2421 MFS family major facilitator transporter, bicyclomycin:cation symporter/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,559,8410CA 8.5% 269.8 / 10.2 82intergenic (+35/+88)bcr/USA300HOU_2421MFS family major facilitator transporter, bicyclomycin:cation symporter/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (45/30);  new base A (2/5);  total (47/35)
Fisher's exact test for biased strand distribution p-value = 1.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01

ATTTAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTG  >  CP000730/2559701‑2559975
                                                                                                                                            |                                                                                                                                      
aTTTAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGAtatc                                                                                                                                        >  1:172786/1‑141 (MQ=255)
    attACATCACTATATATCATTCTTATCTTCATTCCAGCCATTCTATTAGTCAGTCTACCAATCATTTACTCTAAAATGATTAAAAAGCCCCATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGAtatctatc                                                                                                                                    <  1:394192/139‑1 (MQ=255)
    aaTACATCACCATATATGATTATTAACTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGAtatctatc                                                                                                                                    <  2:387307/141‑1 (MQ=255)
     aTACATCACCATATATGGTTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATatctatct                                                                                                                                   <  2:681903/141‑1 (MQ=255)
     aTACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATatctatct                                                                                                                                   <  1:514802/141‑1 (MQ=255)
        cATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTGACAAAGATAGATATCTTTg                                                                                                                                >  2:304083/1‑141 (MQ=255)
         aTCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTGACAAAGATAGATATCTTTgc                                                                                                                               <  2:346682/141‑2 (MQ=255)
         aTCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTGACAAAGATAGATATCTTTgc                                                                                                                               <  2:257169/141‑2 (MQ=255)
         aTCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGt                                                                                                                               >  1:134802/1‑141 (MQ=255)
         aTCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGt                                                                                                                               >  1:517249/1‑141 (MQ=255)
          tCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTc                                                                                                                              <  2:769993/141‑1 (MQ=255)
           cACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTGACAAAGATAGATATCTTTgcta                                                                                                                             <  2:735417/141‑4 (MQ=255)
           cACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCa                                                                                                                             <  1:78781/141‑1 (MQ=255)
             ccATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAgg                                                                                                                           >  2:353638/1‑141 (MQ=255)
              cATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAggg                                                                                                                          <  2:9328/141‑1 (MQ=255)
              cATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAggg                                                                                                                          <  2:9345/141‑1 (MQ=255)
               atatatGATTATTATCTTCATTACAGCCATTCCATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGt                                                                                                                         <  2:326779/141‑1 (MQ=255)
                 atatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTGACAAAGATAGATATCTTTGCTAGGGttc                                                                                                                       <  2:198510/141‑1 (MQ=38)
                  tatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCGGGGttct                                                                                                                      >  2:418354/1‑141 (MQ=255)
                      attattATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTcttctt                                                                                                                  >  1:45498/1‑141 (MQ=255)
                      attattATCTTCAGTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGgtctgccg                                                                                                                  >  2:273326/1‑137 (MQ=255)
                      attattATCTTCAGTACAGCCAGTCTATTAGTCAGTCTACAAATCAGTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATAACTATCTTTGTCAGGGttcttcct                                                                                                                  >  2:273309/1‑139 (MQ=255)
                           tatCTTCATTACAGCCCCTCCATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGa                                                                                                             <  1:205848/141‑1 (MQ=255)
                              cTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTGACAAAGATAGATATCTTTGCTAGGGttctaattatgttgaa                                                                                                          <  1:304083/141‑13 (MQ=25)
                              cTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAAtt                                                                                                          >  1:31432/1‑141 (MQ=255)
                               ttCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTa                                                                                                         >  1:346515/1‑141 (MQ=255)
                               ttCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTa                                                                                                         >  1:334795/1‑141 (MQ=255)
                                     aCAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGAt                                                                                                   <  1:299776/141‑1 (MQ=255)
                                       aGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCg                                                                                                 >  2:271241/1‑141 (MQ=255)
                                        gCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGa                                                                                                >  2:457621/1‑141 (MQ=255)
                                        gCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGa                                                                                                >  2:598593/1‑141 (MQ=255)
                                         ccATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAATCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGaa                                                                                               >  2:629503/1‑141 (MQ=255)
                                               tATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCa                                                                                         >  1:173780/1‑141 (MQ=255)
                                               tATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCa                                                                                         <  1:271241/141‑1 (MQ=255)
                                                aTTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCaa                                                                                        >  2:341433/1‑141 (MQ=255)
                                                aTTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCaa                                                                                        >  2:754248/1‑141 (MQ=255)
                                                aTTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCaa                                                                                        >  2:86970/1‑141 (MQ=255)
                                                aTTAGTCAGTCTACAAATCATATACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAGGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCaa                                                                                        >  2:625541/1‑141 (MQ=255)
                                                 ttAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAAc                                                                                       <  2:320789/141‑1 (MQ=255)
                                                  tAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACt                                                                                      >  2:356883/1‑141 (MQ=255)
                                                  tAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACt                                                                                      >  1:565704/1‑141 (MQ=255)
                                                   agtcagtcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTa                                                                                     <  2:306716/141‑1 (MQ=255)
                                                    gtcagtcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTaa                                                                                    >  1:203619/1‑141 (MQ=255)
                                                      cagtcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTaaaa                                                                                  >  1:406612/1‑141 (MQ=255)
                                                       agtcTACAAATCATTTACTTTAAAAAAATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAAt                                                                                 <  2:540559/141‑1 (MQ=255)
                                                         tcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTa                                                                               >  1:25021/1‑141 (MQ=255)
                                                           tACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACg                                                                             <  1:382440/141‑1 (MQ=255)
                                                                  cATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCAt                                                                      <  2:595399/141‑1 (MQ=255)
                                                                      tACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCa                                                                  <  1:364059/141‑1 (MQ=255)
                                                                        cTTTAAAATGATTAAAAAGCAACATGTCGCATAACGCTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAg                                                                <  2:633079/141‑1 (MQ=255)
                                                                              aaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACAtt                                                          <  1:696625/141‑1 (MQ=255)
                                                                                  aTTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTa                                                      >  1:335667/1‑141 (MQ=255)
                                                                                   ttAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTAt                                                     <  2:727368/141‑1 (MQ=255)
                                                                                     aaaaaGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTc                                                   >  2:567682/1‑141 (MQ=255)
                                                                                       aaaGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAGAATTACGCCTTCATAGCAAGGACATTTCTATTCaa                                                 >  1:741490/1‑141 (MQ=255)
                                                                                       aaaGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCaa                                                 <  1:101556/141‑1 (MQ=255)
                                                                                          gCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCa                                              >  1:23087/1‑141 (MQ=255)
                                                                                           cAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCAc                                             >  1:279452/1‑141 (MQ=255)
                                                                                           cAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCAc                                             >  1:221237/1‑141 (MQ=255)
                                                                                             aCATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCAccc                                           >  2:595853/1‑141 (MQ=255)
                                                                                             aCATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTACCTCTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCAccc                                           >  1:512630/1‑141 (MQ=255)
                                                                                               aTGTTGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTAAGCCTTCATAGCAAGGACATTTCTATTCAATCCCCCtt                                         >  1:130902/1‑141 (MQ=255)
                                                                                               aTGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTACGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCtt                                         >  2:405756/1‑141 (MQ=255)
                                                                                                tGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCttt                                        <  2:594760/141‑1 (MQ=255)
                                                                                                         aagtcactaCATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCAt                               <  1:170424/133‑1 (MQ=255)
                                                                                                          cacTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATc                              <  1:244209/141‑1 (MQ=255)
                                                                                                          cacTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATc                              <  1:207786/141‑1 (MQ=255)
                                                                                                           acTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATcc                             <  1:619937/141‑1 (MQ=255)
                                                                                                              tCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCaaa                          >  2:139361/1‑141 (MQ=255)
                                                                                                              tCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCaaa                          >  2:714116/1‑141 (MQ=255)
                                                                                                                    tAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTg                    >  1:450556/1‑141 (MQ=255)
                                                                                                                     aaTTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGt                   <  2:565704/141‑1 (MQ=255)
                                                                                                                         aGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAata               >  1:630065/1‑141 (MQ=255)
                                                                                                                            aCCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAatatat            >  1:620044/1‑141 (MQ=255)
                                                                                                                            aCCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAatatat            >  1:306359/1‑141 (MQ=255)
                                                                                                                              ccTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGCTCGAATCTTCAACTAAACTTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATAttt          <  1:353638/141‑1 (MQ=255)
                                                                                                                              ccTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATAttt          <  2:134295/141‑1 (MQ=255)
                                                                                                                              ccTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTATGTAATATAttt          >  1:627186/1‑141 (MQ=255)
                                                                                                                               cTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGTCCATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATAtttt         <  2:466050/141‑1 (MQ=255)
                                                                                                                               cTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATAtttt         <  1:71872/141‑1 (MQ=255)
                                                                                                                                tAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATAttttt        <  2:189854/141‑1 (MQ=255)
                                                                                                                                  acAAAGATAGATATCTTTGCTAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCa      >  2:660094/2‑141 (MQ=255)
                                                                                                                                    aaaGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACt    >  2:411873/1‑141 (MQ=255)
                                                                                                                                    aaaGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACt    >  2:176666/1‑141 (MQ=255)
                                                                                                                                     aaGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACtt   <  1:614280/141‑1 (MQ=255)
                                                                                                                                      aGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTg  >  1:668217/1‑141 (MQ=255)
                                                                                                                                      aGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTg  >  2:725034/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                      
ATTTAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTG  >  CP000730/2559701‑2559975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑