Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,615,837 C→T 7.6% V666V (GTG→GTA)  USA300HOU_2480 ← helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,615,8370CT 7.6% 223.5 / 2.2 76V666V (GTG→GTA) USA300HOU_2480helicase
Reads supporting (aligned to +/- strand):  ref base C (21/46);  new base T (5/2);  total (28/48)
Fisher's exact test for biased strand distribution p-value = 8.87e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATATGTTAATTCATTTTTTAAAAGTTCTTCTAAAACTAAACTATCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCTATTTTCAACTTCTTCATAAGCTTTTAATATTAATTTATT  >  CP000730/2615697‑2615977
                                                                                                                                              |                                                                                                                                            
tcatATGTTAATTCATTTTTTTTTTTTTTTTCTAAAACTAAACTATCGATTCTTTTCAATCCATGTTCTATTTGCCTTGAAAAGAAAACCAGATTTTTTTATTCATTTTCCGTCAATAATTTTTC‑‑TTTTTTTTTTTAtctc                                                                                                                                              >  2:116563/1‑141 (MQ=255)
tcatATGTTAATTCATTTTTTAAAAGTTCTTCTAAAACTAAACTATCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATAtctc                                                                                                                                              >  2:201690/1‑141 (MQ=255)
tcatATGTTAATTCATTTTTTAAAAGTTCTTCTAAAACTAAACTATCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATAtctc                                                                                                                                              >  1:425779/1‑141 (MQ=255)
tcatATGTTAATTCATTTTTTAAAAGTTCTTCTAAAACTAAACTATCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATAtctc                                                                                                                                              <  2:339838/141‑1 (MQ=255)
  atatGTTAATTCATTTTTTAAAAGTTCTTCTAAAACTAAACTATCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCAc                                                                                                                                            <  1:722641/141‑1 (MQ=255)
   tatGTTAATTCATTTTTTAAAAGTTCTTCTAAAACTAAACTATCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACt                                                                                                                                           >  2:695322/1‑141 (MQ=255)
            tCATTTTTTAAAAGTTCTTCTAAAACTAAACTATCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCg                                                                                                                                  <  2:6251/141‑1 (MQ=255)
             cATTTTTTAAAAGTTCTTCTAAAACTAAACTATCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGACCTTTAGATTCGTTTTCCGTCAACAACGTATC‑‑TATTTTTTTATATCTGACTAAGTACCCGt                                                                                                                                 >  2:427347/1‑141 (MQ=255)
                tttttAAAAGTTCTTCTAAAACTAAACTATCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGtaat                                                                                                                              <  2:610123/141‑1 (MQ=255)
                  tttAAAAGTTCTTCTAAAACTAAACTATCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGtaataa                                                                                                                            <  1:342095/141‑1 (MQ=255)
                   ttAAAAGTTCTTCTAAAACTAAACTATCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTaataat                                                                                                                           >  1:303272/1‑141 (MQ=255)
                   ttAAAAGTTCTTCTAAAACTAAACTATCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTaataat                                                                                                                           >  1:22035/1‑141 (MQ=255)
                       aaGTTTTTCTAAAACTAAACTATCGATTCTTTTTATTTCTGGTGCTATTTGCGTCGAAAAGAAAACCACATTTTTAGATTCATTTTCCTTCAATAATGTATC‑‑TACTTTTTTATTTTTCACTTAGAACTCCTAATAATTACt                                                                                                                       >  2:300478/1‑141 (MQ=255)
                       aaGTTCTTCTAAAACTAAACTATCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTTACTAAGAACTCGTAATAATTACt                                                                                                                       >  2:637015/1‑141 (MQ=255)
                        aGTTCTTCTAAAACTAAACTATCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTa                                                                                                                      >  1:515307/1‑141 (MQ=255)
                        aGTTCTTCTAAAACTAAACTATCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTa                                                                                                                      <  2:709825/141‑1 (MQ=255)
                          ttcttcTAAAACTAAACTATCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTaaa                                                                                                                    <  2:305264/141‑1 (MQ=255)
                           tcttctAAAACTAAACTATCGATTCTTTTCAATCCATGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAAt                                                                                                                   <  2:665517/141‑1 (MQ=255)
                             ttctAAAACTAAACTATCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAAttt                                                                                                                 <  2:695532/141‑1 (MQ=255)
                               ctAAAACTAAACTATCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAg                                                                                                               >  1:467673/1‑141 (MQ=255)
                               ctAAAACTAAACTATCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAg                                                                                                               >  2:120028/1‑141 (MQ=255)
                                tAAAACTAAACTATCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATTATTACTAAATTTAGa                                                                                                              <  2:287301/141‑1 (MQ=255)
                                tAAAACTAAACTATCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGa                                                                                                              <  1:473183/141‑1 (MQ=255)
                                   aactaaactaTCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGaaaa                                                                                                           <  1:695322/141‑1 (MQ=255)
                                    actaaactaTCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAat                                                                                                          <  2:207143/141‑1 (MQ=255)
                                     gttttgccatgATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAata                                                                                                         <  1:603120/131‑1 (MQ=255)
                                      taaactaTCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAatat                                                                                                        <  1:335309/141‑1 (MQ=255)
                                       aaactaTCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAAtata                                                                                                       >  2:252503/1‑141 (MQ=255)
                                        aactaTCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGAGTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACCAAGAACTCGTAATAATTACTAAATTTAGAAAATATaa                                                                                                      >  1:398703/1‑141 (MQ=255)
                                         actaTCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTTTTTTTTATTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAAc                                                                                                     >  2:467906/1‑141 (MQ=255)
                                          ctaTCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACg                                                                                                    <  1:451325/141‑1 (MQ=255)
                                            aTCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGCACTCGTAATAATTACTAAATTTAGAAAATATAACGCt                                                                                                  <  1:567363/141‑1 (MQ=255)
                                               gATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTTTTTTTTTTTTCCGTCAATAATGTATC‑‑TCTTTTTTTATATTTCACTAAGCACTCCTAATACTTACTACATTTATAACATATAACGCtttg                                                                                               >  2:743061/1‑139 (MQ=255)
                                               gATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTgg                                                                                               <  2:243681/141‑1 (MQ=255)
                                                 ttCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTCGATTCATTATCCGTCCATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGAt                                                                                             >  2:704751/1‑141 (MQ=255)
                                                        cAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGa                                                                                      <  1:250003/141‑1 (MQ=255)
                                                         aaTCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGaa                                                                                     >  1:216558/1‑141 (MQ=255)
                                                           tCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAAtg                                                                                   <  1:175225/141‑1 (MQ=255)
                                                            ccAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAAtgt                                                                                  <  1:309403/141‑1 (MQ=255)
                                                             cAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAAAGATTGGATCTATAGAAtgtt                                                                                 >  2:343464/1‑141 (MQ=255)
                                                              aGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAAtgttg                                                                                >  2:208297/1‑141 (MQ=255)
                                                               ggTGCTATTTGTCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAAAGCATC‑‑TATTTTTTTATACCTTAATAAAAATTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAAtgttgt                                                                               >  2:651117/1‑141 (MQ=255)
                                                               ggTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAAtgttgt                                                                               <  1:481026/141‑1 (MQ=255)
                                                                   cTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTa                                                                           <  2:449401/141‑1 (MQ=255)
                                                                    tATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTTTTTTTTTTTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGTATGTTTTTGTAt                                                                          >  2:459296/1‑141 (MQ=255)
                                                                      tttGTCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAAAGCATC‑‑TATTTTTTTATACCTTAATAAAAATTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTAtaa                                                                        <  1:651117/141‑3 (MQ=255)
                                                                      tttGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGa                                                                        <  1:493841/141‑1 (MQ=255)
                                                                      tttGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGa                                                                        <  1:528548/141‑1 (MQ=255)
                                                                           cTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATcc                                                                   <  2:181848/141‑1 (MQ=255)
                                                                             tGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAAACCAt                                                                 >  2:774241/1‑141 (MQ=255)
                                                                               aaaagaaaaCCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTa                                                               >  1:329347/1‑141 (MQ=255)
                                                                               aaaagaaaaCCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTa                                                               <  2:549385/141‑1 (MQ=255)
                                                                                  agaaaaCCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTa                                                            <  1:94881/141‑1 (MQ=255)
                                                                                     aaaCCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTTTTTTTTTTTAATAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGTTATCCATTAGTAACg                                                         >  2:52791/1‑141 (MQ=255)
                                                                                      aaCCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACgg                                                        <  2:688718/141‑1 (MQ=255)
                                                                                       aCCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTAACTAAGAACTCGTAATAATTACTAAATTTAGAAAATGTAACGGTTCGACCTGTAGATTGTTGTAGTATGAAATCCATTAGTAATGGc                                                       >  1:424930/1‑141 (MQ=255)
                                                                                        ccAGATTTTTAGATTCATTTTCCGTCAATAATGTATCTATATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACgg                                                        <  2:319443/141‑1 (MQ=255)
                                                                                          aGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCAtg                                                    <  2:734926/141‑1 (MQ=255)
                                                                                               tttAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTcc                                               >  2:62814/1‑141 (MQ=255)
                                                                                                ttAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTccc                                              <  1:630068/141‑1 (MQ=255)
                                                                                                 tAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCa                                             >  2:676316/1‑141 (MQ=255)
                                                                                                     ttCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCt                                         <  1:609398/141‑1 (MQ=255)
                                                                                                         ttttCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCTAttt                                     <  2:24036/141‑1 (MQ=255)
                                                                                                           ttCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCTATTTTc                                   <  1:187854/141‑1 (MQ=255)
                                                                                                            tCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCTATTTTCa                                  <  1:216880/141‑1 (MQ=255)
                                                                                                                tCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCTATTTTCAActt                              <  1:583409/141‑1 (MQ=255)
                                                                                                                  aataatGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCTATTTTCAActtct                            <  2:689999/141‑1 (MQ=255)
                                                                                                                    taatGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCTATTTTCAACttcttc                          <  2:365016/141‑1 (MQ=255)
                                                                                                                      atGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCTATTTTCAACTTCTTCAt                        <  1:343372/141‑1 (MQ=255)
                                                                                                                      aagcaTC‑‑TATTTTTTTATACCTTAATAAAAATTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATAAAATCCATTAGTAACGGCATGTGTCCCAATCTATTTTCAACTTCTTCAt                        <  2:439384/137‑1 (MQ=255)
                                                                                                                       tGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCTATTTTCAACTTCTTCATa                       <  2:444125/141‑1 (MQ=255)
                                                                                                                        gTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCTATTTTCAACTTCTTCATaa                      <  2:320678/141‑1 (MQ=255)
                                                                                                                         tATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCTATTTTCAACTTCTTCATAAg                     <  1:219839/141‑1 (MQ=255)
                                                                                                                            c‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCTATTTTCAACTTCTTCATAAGCtt                  >  2:599063/1‑141 (MQ=255)
                                                                                                                               tATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCTATTTTCAACTTCTTCATAAGCttt                 <  2:3918/141‑1 (MQ=255)
                                                                                                                                aTTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCTATTTTCAACTTCTTCATAAGCtttt                <  2:425779/141‑1 (MQ=255)
                                                                                                                                       tataTCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCTATTTTCAACTTCTTCATAAGCTTTTAATATTa         >  1:226621/1‑141 (MQ=255)
                                                                                                                                        atatCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCTATTTTCAACTTCTTCATAAGCTTTTAATATTaa        <  1:22524/141‑1 (MQ=255)
                                                                                                                                        atatCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCTATTTTCAACTTCTTCATAAGCTTTTAATATTaa        <  2:762320/141‑1 (MQ=255)
                                                                                                                                         tatCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCTATTTTCAACTTCTTCATAAGCTTTTAATATTAat       >  1:309119/1‑141 (MQ=255)
                                                                                                                                           tctcACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCTATTTTCAACTTCTTCATAAGCTTTTAATATTAattt     <  2:684023/141‑1 (MQ=255)
                                                                                                                                            ctcACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCTATTTTCAACTTCTTCATAAGCTTTTAATATTAattta    >  2:59924/1‑141 (MQ=255)
                                                                                                                                              cACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCTATTTTCAACTTCTTCATAAGCTTTTAATATTAatttatt  <  1:716049/141‑1 (MQ=255)
                                                                                                                                              cACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCTATTTTCAACTTCTTCATAAGCTTTTAATATTAatttatt  <  1:544491/141‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                            
TCATATGTTAATTCATTTTTTAAAAGTTCTTCTAAAACTAAACTATCGATTCTTTTCAATCCAGGTGCTATTTGCCTTGAAAAGAAAACCAGATTTTTAGATTCATTTTCCGTCAATAATGTATC‑‑TATTTTTTTATATCTCACTAAGAACTCGTAATAATTACTAAATTTAGAAAATATAACGCTTGGATCTATAGAATGTTGTTGTATGAAATCCATTAGTAACGGCATGTGTCCCAATCTATTTTCAACTTCTTCATAAGCTTTTAATATTAATTTATT  >  CP000730/2615697‑2615977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑