Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,655,403	T→C	5.3%	N38S (AAT→AGT)	yahD ←	possible hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,655,403	0	T	C	5.3%	235.5 / 2.8	76	N38S (AAT→AGT)	yahD	possible hydrolase
Reads supporting (aligned to +/- strand):  ref base T (41/31);  new base C (2/2);  total (43/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

CTTCTTTAATGAACGTCAACAATTCTTGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTGTCCTTCTCTAAAAATATGTTCCATCACAAATCCTCCTCATTCCAATTAACCA  >  CP000730/2655266‑2655543                                                                                                                                          |                                                                                                                                             cttcttTAATGAACGTCAACAATTCTTGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAAtttt                                                                                                                                           >  1:204747/1‑141 (MQ=255)      ttAATGAACGTCAACAATTCTTGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAAGTTGTCCTCTAATACTTAACAAGCGATAATTttcattc                                                                                                                                      >  2:447731/1‑141 (MQ=255)       tAATGAACGTCAACAATTCTTGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTttcattca                                                                                                                                     >  1:764648/1‑141 (MQ=255)        aaTGAACGTCAACAATTCTTGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCaa                                                                                                                                    <  1:546959/141‑1 (MQ=255)          tGAACGTCAACAATTCTTGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATg                                                                                                                                  >  1:682036/1‑141 (MQ=255)           gAACGTCAACAATTCTTGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGc                                                                                                                                 <  1:203390/141‑1 (MQ=255)              cGTCAACAATTCTTGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTc                                                                                                                              >  1:519092/1‑141 (MQ=255)               gTCAACAATTCTTGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCg                                                                                                                             >  1:515965/1‑141 (MQ=255)                tCAACAATTCTTGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGc                                                                                                                            <  1:232587/141‑1 (MQ=255)                  aacaaTTCTTGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCt                                                                                                                          >  1:189873/1‑141 (MQ=255)                   acaaTTCTTGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTa                                                                                                                         >  1:784314/1‑141 (MQ=255)                   acaaTTCTTGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTa                                                                                                                         >  1:103281/1‑141 (MQ=255)                    caaTTCTTGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTaa                                                                                                                        >  1:439692/1‑141 (MQ=255)                    caaTTCTTGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCATCTAATACTTAACAAGTGATAATTTTCATTAAATGCTTCGCCTaa                                                                                                                        >  1:533689/1‑141 (MQ=255)                      aTTCTTGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACg                                                                                                                      >  2:246430/1‑141 (MQ=255)                        tCTTGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGt                                                                                                                    >  1:494841/1‑141 (MQ=255)                          ttGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGtaa                                                                                                                  >  1:759959/1‑141 (MQ=255)                           tgaccACGAAATGCCAAATCTTCTTCATCAGAAACCCCTTCACCAATACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACGTGCCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCGTCGCCTAACGGtaat                                                                                                                 <  2:25929/138‑1 (MQ=255)                              ccACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAACTTTCATTCAATGCTTCGCCTAACGGTAATaaa                                                                                                              >  2:687837/1‑141 (MQ=255)                              ccACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAACTTTCATTCAATGCTTCGCCTAACGGTAATaaa                                                                                                              >  2:694812/1‑141 (MQ=255)                              ccACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTCTCCTCTAACACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATaaa                                                                                                              >  2:535319/1‑141 (MQ=255)                                aCGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATc                                                                                                            <  2:121728/141‑1 (MQ=255)                                aCGAAATGCCAAATCGTCTGCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATc                                                                                                            <  1:261291/141‑1 (MQ=255)                                 cGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCg                                                                                                           <  1:765605/141‑1 (MQ=255)                                 cGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCg                                                                                                           <  2:628326/141‑1 (MQ=255)                                  gAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGa                                                                                                          <  2:251336/141‑1 (MQ=255)                                      tGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACtc                                                                                                      >  1:456641/1‑141 (MQ=255)                                        ccAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACtcat                                                                                                    >  1:464766/1‑141 (MQ=255)                                        ccAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACtcat                                                                                                    >  2:764385/1‑141 (MQ=255)                                         cAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACtcatc                                                                                                   >  1:64936/1‑141 (MQ=255)                                            aTCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAAAGGTTAATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATAGAACTCATcacc                                                                                                >  2:552526/1‑141 (MQ=255)                                              cttcttCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATcaccac                                                                                              <  2:629138/141‑1 (MQ=255)                                               ttcttcATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCaccacc                                                                                             <  1:502764/141‑1 (MQ=255)                                                tcttcATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCt                                                                                            >  1:104455/1‑141 (MQ=255)                                                tcttcATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCt                                                                                            <  2:384736/141‑1 (MQ=255)                                                  ttcATCATAAACCCAATCACCACCACCTCTGCAACTACTGTTCACCCCTTTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGt                                                                                          <  1:424698/141‑1 (MQ=255)                                                  gacatcatAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGt                                                                                          <  2:564951/139‑1 (MQ=255)                                                      tcatAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCa                                                                                      <  2:511627/141‑1 (MQ=255)                                                      tcatAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCa                                                                                      <  2:33786/141‑1 (MQ=255)                                                          aaaCACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCa                                                                                  >  1:778424/1‑141 (MQ=255)                                                             cacCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCaata                                                                               >  2:44632/1‑141 (MQ=255)                                                             cacCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCaata                                                                               >  1:617867/1‑141 (MQ=255)                                                                   cACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTa                                                                         >  1:655678/1‑141 (MQ=255)                                                                     ccAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAgt                                                                       >  1:1045/1‑141 (MQ=255)                                                                            gTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGgcc                                                                >  2:636753/1‑140 (MQ=255)                                                                             tttGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGgcgc                                                               <  1:200408/141‑1 (MQ=255)                                                                               tGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCAc                                                             >  1:109964/1‑141 (MQ=255)                                                                               tGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAACTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCAc                                                             <  1:687837/141‑1 (MQ=255)                                                                               tGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAACTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCAc                                                             <  1:694812/141‑1 (MQ=255)                                                                                  aaTAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCAt                                                          <  1:166275/141‑1 (MQ=255)                                                                                   aTAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATCACTCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCAtt                                                         <  1:128937/141‑1 (MQ=255)                                                                                   aTAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCAtt                                                         <  1:412998/141‑1 (MQ=255)                                                                                    tAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCAttt                                                        <  1:288919/141‑1 (MQ=255)                                                                                      aCGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTg                                                      >  1:649872/1‑141 (MQ=255)                                                                                      aCGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTGAACAAGTGATAATTTTCATTCGATGCTTCGCCTAACGGTAATAAATCGAACTCATCCCCACCTGTACCATGNAATAATATTAGTGTTGGCGCACCATTTTg                                                      <  1:581772/141‑1 (MQ=255)                                                                                             aTCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTGTCCTtct                                               >  2:422551/1‑141 (MQ=255)                                                                                              tCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTGTCCTtctc                                              >  2:488971/1‑141 (MQ=255)                                                                                                  aTTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCAtttaaaaaaaaaaaaaa                                          <  2:387105/141‑15 (MQ=255)                                                                                                  aTTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTGTCCTTCTCTaaa                                          >  2:456802/1‑141 (MQ=255)                                                                                                   ttttCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTGTCCTTCTCTaaaa                                         <  1:117981/141‑1 (MQ=255)                                                                                                   ttttCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTGTCCTTCTCTaaaa                                         >  2:785508/1‑141 (MQ=255)                                                                                                      tCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGTCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTGTCCTTCTCTAAAAata                                      <  2:625467/141‑1 (MQ=255)                                                                                                       cTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTGTCCTTCTCTAAAAatat                                     <  1:429239/141‑1 (MQ=255)                                                                                                        tGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAAATAATCCCAACATGTACCCTGCAATAATATTAGTGTTGGCGCACCATTTTGTCCTTCTCTAAAAATATg                                    >  1:467061/1‑141 (MQ=255)                                                                                                         gAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTGTTTTTTTCTATAAATATGt                                   >  2:423091/1‑141 (MQ=255)                                                                                                            aCTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTGTCCTTCTCTAAAAATATGTTcc                                <  2:230662/141‑1 (MQ=255)                                                                                                                gTCCTCTAATACTTAGCAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTGTCCTTCTCTAAAAATATGTTCCATca                            <  1:652426/141‑1 (MQ=255)                                                                                                                gTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTGTCCTTCTCTAAAAATATGTTCCATca                            <  1:499505/141‑1 (MQ=255)                                                                                                                   ctctAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTGTCCTTCTCTAAAAATATGTTCCATCACaa                         >  2:563228/1‑141 (MQ=255)                                                                                                                          aCTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCGCCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTGTCCTTCTCTAAAAATATGTTCCATCACAAAtcctcc                  >  1:363483/1‑141 (MQ=255)                                                                                                                            ttAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATCATATTAGTGGTGGCGCACCATTTTGTCCTCCTCTAAAAATATGTTCCATCACAAATCctcctc                >  1:761344/1‑141 (MQ=255)                                                                                                                            ttAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTGTCCTTCTCTAAAAATATGTTCCATCACAAATCctcctc                <  2:74430/141‑1 (MQ=255)                                                                                                                            ttAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTGTCCTTCTCTAAAAATATGTTCCATCACAAATCctcctc                >  2:756904/1‑141 (MQ=255)                                                                                                                             tAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTGTCCTTCTCTAAAAATATGTTCCATCACAAATCCTCCTCa               <  2:7441/141‑1 (MQ=255)                                                                                                                              aaCAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTGTCCTTCTCTAAAAATATGTTCCATCACAAATCCTCCTCAt              >  2:221740/1‑141 (MQ=255)                                                                                                                                     gATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTGTCCTTCTCTAAAAATATGTTCCATCACAAATCCTCCTCATTCCAAtt       >  2:46077/1‑141 (MQ=255)                                                                                                                                       tAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTGTCCTTCTCTAAAAATATGTTCCATCACAAATCCTCCTCATTCCAATTaa     >  2:150598/1‑141 (MQ=255)                                                                                                                                          ttttCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTGTCCTTCTCTAAAAATATGTTCCATCACAAATCCTCCTCATTCCAATTAACCa  >  1:676957/1‑141 (MQ=255)                                                                                                                                          ttttCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTGTCCTTCTCTAAAAATATGTTCCATCACAAATCCTCCTCATTCCAATTAACCa  >  2:645980/1‑141 (MQ=255)                                                                                                                                          |                                                                                                                                             CTTCTTTAATGAACGTCAACAATTCTTGTCCACGAAATGCCAAATCTTCTTCATCATAAACACCTTCACCAAGACGTTTGAAATAACGGTTCATCCCATTTTCTGAAACTTGTCCTCTAATACTTAACAAGTGATAATTTTCATTCAATGCTTCGCCTAACGGTAATAAATCGAACTCATCACCACCTGTACCATGCAATAATATTAGTGTTGGCGCACCATTTTGTCCTTCTCTAAAAATATGTTCCATCACAAATCCTCCTCATTCCAATTAACCA  >  CP000730/2655266‑2655543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑