Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,821,539	T→C	5.1%	L73L (TTA→TTG)	ywrF ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,821,539	0	T	C	5.1%	249.3 / 3.2	79	L73L (TTA→TTG)	ywrF	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (40/35);  new base C (2/2);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

TTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCATTTACTGATGCATCTTGATTTAATGTTGTAACAAAAGCTA  >  CP000730/2821400‑2821678                                                                                                                                            |                                                                                                                                            ttGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTa                                                                                                                                            <  1:20531/141‑1 (MQ=255)     agagGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTACAGTCCGTTCTATATTTAATGc                                                                                                                                        >  2:371093/1‑141 (MQ=255)          ttAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTAt                                                                                                                                   >  2:60112/1‑141 (MQ=255)          ttAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTAt                                                                                                                                   >  2:230060/1‑141 (MQ=255)            aaaaGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCt                                                                                                                                 <  2:411623/141‑1 (MQ=255)                 tGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTtct                                                                                                                            <  1:582133/141‑1 (MQ=255)                   aTAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTctct                                                                                                                          >  1:226712/1‑141 (MQ=255)                        tCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTAcct                                                                                                                     <  2:504124/141‑1 (MQ=255)                         cTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTAcctc                                                                                                                    >  1:249870/1‑141 (MQ=255)                         cTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTAcctc                                                                                                                    >  2:747563/1‑141 (MQ=255)                           gTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTAcctcct                                                                                                                  >  2:381292/1‑141 (MQ=255)                            tACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTg                                                                                                                 <  1:773764/141‑1 (MQ=255)                              cGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCa                                                                                                               <  1:196962/141‑1 (MQ=255)                              cGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCa                                                                                                               >  1:757161/1‑141 (MQ=255)                               gACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCAc                                                                                                              <  1:166405/141‑1 (MQ=255)                                actcaactcaCTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACg                                                                                                             <  2:574240/141‑1 (MQ=255)                                actcaactcaCTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACg                                                                                                             <  1:573810/141‑1 (MQ=255)                                       tcaCTGTCACCATATGCTAAGGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACt                                                                                                      <  1:527574/141‑1 (MQ=255)                                         aCTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGc                                                                                                    >  1:749978/1‑141 (MQ=255)                                          cTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCa                                                                                                   >  2:372714/1‑141 (MQ=255)                                           tGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCaa                                                                                                  >  1:533382/1‑141 (MQ=255)                                            gTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAAt                                                                                                 >  1:188319/1‑141 (MQ=255)                                             tCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATa                                                                                                >  2:87181/1‑141 (MQ=255)                                             tCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTCAAGGCAGTATCTTTTCTCTTACCTCCTGCAc                                                                                                              >  1:64949/1‑127 (MQ=255)                                             tCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTCAAGGCAGTATCTTTTCTCTTACCTCCTGCAc                                                                                                              <  2:64949/127‑1 (MQ=255)                                                  atatGCTAACGGCGCTGCAGTCTCAGTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAAt                                                                                           >  1:101145/1‑141 (MQ=255)                                                   tatGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATc                                                                                          <  2:167387/141‑1 (MQ=255)                                                      gCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCAtt                                                                                       >  1:223814/1‑141 (MQ=255)                                                        tAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTgg                                                                                     >  2:545957/1‑141 (MQ=255)                                                         aaCGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGc                                                                                    >  2:701339/1‑141 (MQ=255)                                                          aCGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCg                                                                                   >  2:669647/1‑141 (MQ=255)                                                           cGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCgg                                                                                  <  2:488308/141‑1 (MQ=255)                                                           cGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCgg                                                                                  <  2:247279/141‑1 (MQ=255)                                                             gcgcTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCAtccgccaat                                                                                <  2:88229/141‑9 (MQ=255)                                                                 tGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGatt                                                                            >  2:47013/1‑141 (MQ=255)                                                                     gTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGAttatta                                                                        >  2:396024/1‑141 (MQ=255)                                                                        tcATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACa                                                                     >  2:432947/1‑141 (MQ=255)                                                                          aTTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAaaaaa                                                                   <  2:266861/141‑2 (MQ=255)                                                                          aTTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAat                                                                   >  2:491727/1‑141 (MQ=255)                                                                          aTTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAat                                                                   >  2:491725/1‑141 (MQ=255)                                                                               tatCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTaa                                                              <  1:145656/141‑1 (MQ=255)                                                                                atCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTaaa                                                             <  1:432947/141‑1 (MQ=255)                                                                                 tCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTaaaa                                                            >  1:308678/1‑141 (MQ=255)                                                                                  cTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTaaaaa                                                           <  2:487018/141‑1 (MQ=255)                                                                                   ttGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTCGCGGATGATTATTAACAATATTaaaaaa                                                          >  2:496690/1‑141 (MQ=255)                                                                                    tGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAg                                                         >  1:477188/1‑141 (MQ=255)                                                                                      aaCATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTAt                                                                                                                                   <  2:126865/65‑1 (MQ=255)                                                                                      aaCATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTAt                                                                                                                                   >  1:126865/1‑65 (MQ=255)                                                                                      aaCATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCt                                                       <  2:784987/141‑1 (MQ=255)                                                                                                  gTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGtgctgc                                           <  2:344228/141‑1 (MQ=255)                                                                                                  gTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGtgctgc                                           >  1:620453/1‑141 (MQ=255)                                                                                                   tCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCa                                          <  1:230334/141‑1 (MQ=255)                                                                                                     tGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAGTGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCAtt                                        <  2:384938/141‑1 (MQ=255)                                                                                                     tGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTCCCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCAtt                                        <  1:688790/141‑1 (MQ=255)                                                                                                     tGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCAtt                                        <  2:679606/141‑1 (MQ=255)                                                                                                      gTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTCAAGGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAACCATTGGCGGATGGTTATTAACAata                                                                  <  1:227535/114‑1 (MQ=255)                                                                                                      gTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTCAAGGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAACCATTGGCGGATGGTTATTAACAata                                                                  >  2:227535/1‑114 (MQ=255)                                                                                                        aaTATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCATTTAc                                     >  2:632082/1‑141 (MQ=255)                                                                                                         atatGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCATTTACt                                    <  1:638479/141‑1 (MQ=255)                                                                                                            tGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCATTTACTGAt                                 <  1:334623/141‑1 (MQ=255)                                                                                                            tGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCATTTACTGAt                                 <  1:19631/141‑1 (MQ=255)                                                                                                              cacaACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCATTTACTGATGc                               >  1:25139/1‑141 (MQ=255)                                                                                                               acaacaAAGTCCCGAGACCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTACATGGTGCTGCATTTACTGATGCa                              >  2:122530/1‑141 (MQ=255)                                                                                                                 aacaaAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCATTTACTGATGCATc                            <  1:701339/141‑1 (MQ=255)                                                                                                                   caaAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCATTTACTGATGCATCtt                          <  1:284449/141‑1 (MQ=255)                                                                                                                    aaaGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCAt                                         <  2:362256/125‑1 (MQ=255)                                                                                                                    aaaGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCAt                                         >  1:362256/1‑125 (MQ=255)                                                                                                                     aaGTCTCCCGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCATTTACTGATGCATCTTGa                        <  2:101145/141‑1 (MQ=255)                                                                                                                       gTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCATTTACTGATGCATCTTGAtt                      >  1:189007/1‑141 (MQ=255)                                                                                                                       gTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCATTTACTGATGCATCTTGAtt                      <  2:465382/141‑1 (MQ=255)                                                                                                                       gTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCATTTACTGATGCATCTTGAtt                      >  2:314176/1‑141 (MQ=255)                                                                                                                        tctcCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCACCGACGTATGCATCTTGAttt                     >  1:437947/1‑141 (MQ=255)                                                                                                                          tcCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCATTTACTGATGCATCTTGATTTaa                   >  1:156374/1‑141 (MQ=255)                                                                                                                          tcCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCATTTACTGATGCATCTTGATTTaa                   <  1:327426/141‑1 (MQ=255)                                                                                                                            cAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCATTTACTGATGCATCTTGATTTAAtg                 >  1:148720/1‑141 (MQ=255)                                                                                                                            cAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCATTTACTGATGCATCTTGATTTAAtg                 >  2:291960/1‑141 (MQ=255)                                                                                                                              gTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCATTTACTGATGCATCTTGATTTAAtgtt               >  1:767556/1‑141 (MQ=255)                                                                                                                               tCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCATTTACTGATGCATCTTGATTTAAtgttg              <  1:752222/141‑1 (MQ=255)                                                                                                                                 cGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCATTTACTGATGCATCTTGATTTAATGTTGTa            >  1:163563/1‑141 (MQ=255)                                                                                                                                           ttAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCATTTACTGATGCATCTTGATTTAATGTTGTAACAAAAGCta  <  2:458415/141‑1 (MQ=255)                                                                                                                                           ttAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCATTTACTGATGCATCTTGATTTAATGTTGTAACAAAAGCta  <  1:367717/141‑1 (MQ=255)                                                                                                                                            |                                                                                                                                            TTGTAGATGTTAAAAGTGATAATTCTGTACGACTCAACTCACTGTCACCATATGCTAACGGCGCTGCAGTCTCATTAATATCTTGAACATTATCTTCGTCTGTAATATGCACAACAAAGTCTCCAGTCCGTTCTATATTTAATGCAGTATCTTTTCTCTTACCTCCTGCACGTTGAACTGCAATAGCAATCATTGGCGGATGATTATTAACAATATTAAAAAAGCTAAATGGTGCTGCATTTACTGATGCATCTTGATTTAATGTTGTAACAAAAGCTA  >  CP000730/2821400‑2821678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑