Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 39,653 C→A 7.9% S494I (AGT→ATT)  mecA ← penicillin binding protein 2 prime

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073039,6530CA 7.9% 285.7 / 9.3 87S494I (AGT→ATT) mecApenicillin binding protein 2 prime
Reads supporting (aligned to +/- strand):  ref base C (40/40);  new base A (2/5);  total (42/45)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

ATTTTCTAATGCGCTATAGATTGAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTACCACT  >  CP000730/39514‑39793
                                                                                                                                           |                                                                                                                                            
attTTCTAATGCGCTATAGATTGAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACt                                                                                                                                             <  1:579272/141‑1 (MQ=255)
  tttCTAATGCGCTATAGATTGAAAGGATCTGTACTGGGTTATTCAGTATTTCACCTTCTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACtgg                                                                                                                                           <  2:768458/141‑3 (MQ=255)
  tttCTAATGCGCTATAGATTGAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTg                                                                                                                                           >  1:247667/1‑141 (MQ=255)
   ttCTAATGCGCTATAGATTGAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTgg                                                                                                                                          <  2:598053/141‑1 (MQ=255)
      tAATGCGCTATAGATTGAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGtat                                                                                                                                       >  1:340536/1‑141 (MQ=255)
       aaTGCGCTATAGATTGAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGtata                                                                                                                                      >  1:2994/1‑141 (MQ=255)
       aaTGCGCTATAGATTGAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGAATTATAAAATGGATAATCACTTGGtata                                                                                                                                      >  2:681994/1‑141 (MQ=255)
                taGATTGAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCa                                                                                                                             <  1:108995/141‑1 (MQ=255)
                taGATTGAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCAATTGGTATATCTTCACCa                                                                                                                             <  1:168484/141‑1 (MQ=255)
                  gATTGAAAGGATCTGTACTGGGTTAATCAGTAATTCACCTTGTGCGTAACCTGAATCAGCTAATAATAGCTCATTATCTAAATATTTGTTTGAAATTTGAGCATTATATAATGGATTCTCCCTGGGTATATCtcaacccgc                                                                                                                           >  2:540339/1‑133 (MQ=255)
                     tGAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACAcc                                                                                                                        <  2:492529/141‑1 (MQ=255)
                      gAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCt                                                                                                                       <  2:522732/141‑1 (MQ=255)
                         aGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGt                                                                                                                    >  2:141209/1‑141 (MQ=255)
                             tCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGttttt                                                                                                                >  2:637359/1‑141 (MQ=255)
                               tGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTc                                                                                                              >  1:280112/1‑141 (MQ=255)
                                gTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAGATTTGAGCCTTATAAGATGGATGCCCCCTTGTTATCTCTTCACCGACACCTAGTTTTTTCa                                                                                                             >  1:546071/1‑141 (MQ=255)
                                gTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCa                                                                                                             >  2:173638/1‑141 (MQ=255)
                                gTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCa                                                                                                             >  2:494149/1‑141 (MQ=255)
                                   cTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGc                                                                                                          >  1:558208/1‑141 (MQ=255)
                                    tGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGcc                                                                                                         <  2:66865/141‑1 (MQ=255)
                                    tGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGcc                                                                                                         >  2:53516/1‑141 (MQ=255)
                                     gggTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCt                                                                                                        >  2:49646/1‑141 (MQ=255)
                                        ttAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCtttt                                                                                                     <  2:149351/141‑1 (MQ=255)
                                        ttAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCtttt                                                                                                     <  2:775030/141‑1 (MQ=255)
                                        ttAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCtttt                                                                                                     <  2:101962/141‑1 (MQ=255)
                                            tCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCaa                                                                                                 >  1:147743/1‑141 (MQ=255)
                                             taGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCaaa                                                                                                <  2:83760/140‑1 (MQ=255)
                                             cAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCaaa                                                                                                >  1:445509/1‑141 (MQ=255)
                                             cAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCaaa                                                                                                <  2:643405/141‑1 (MQ=255)
                                               gTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTGTCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAAtt                                                                                              >  2:771794/1‑141 (MQ=255)
                                                tATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTTTTTTTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAAttt                                                                                             >  2:251293/1‑141 (MQ=255)
                                                     cACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTAc                                                                                        <  2:202694/141‑1 (MQ=255)
                                                     cACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTAc                                                                                        >  1:699509/1‑141 (MQ=255)
                                                      aCCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACt                                                                                       >  2:326479/1‑141 (MQ=255)
                                                       ccTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTTTTTTTAATTTGAGCATTATAAAATGGATAATCACTTTGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCttcttc                                                                                      >  2:12367/1‑137 (MQ=255)
                                                         ttGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGcc                                                                                    <  1:88921/141‑1 (MQ=255)
                                                           gTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTTTTTTTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTa                                                                                  >  2:213988/1‑141 (MQ=255)
                                                           gTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTa                                                                                  <  2:40908/141‑1 (MQ=255)
                                                           gTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTa                                                                                  <  2:147743/141‑1 (MQ=255)
                                                           gTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCAATTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTa                                                                                  <  2:555435/141‑1 (MQ=255)
                                                            tCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTTTTTTTTATTTGAGCATTATAAAATGGATCATCACTTGTTATGTTTTCACCAGCTCCTAGTTTTTTCATTCCCTTTTCAACTTTCTTTCCGCCTaa                                                                                 >  2:720090/1‑141 (MQ=255)
                                                            tCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTaa                                                                                 <  2:131694/141‑1 (MQ=255)
                                                                tAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCg                                                                             <  1:620253/141‑1 (MQ=255)
                                                                 aaCCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGa                                                                            >  1:776655/1‑141 (MQ=255)
                                                                 aaCCCGAATCAGCTAATAATATTTCATTATCTAAATTTTTTTTTGTTATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGa                                                                            >  2:179344/1‑141 (MQ=255)
                                                                  aCCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAg                                                                           >  2:642776/1‑141 (MQ=255)
                                                                  aCCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAg                                                                           >  1:610738/1‑141 (MQ=255)
                                                                   ccTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGt                                                                          <  1:517498/141‑1 (MQ=255)
                                                                   ccTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGt                                                                          <  1:215954/141‑1 (MQ=255)
                                                                   ccTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGt                                                                          <  1:369398/141‑1 (MQ=255)
                                                                    cTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTg                                                                         <  1:494149/141‑1 (MQ=255)
                                                                       aaTCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTa                                                                      >  1:118349/1‑141 (MQ=255)
                                                                        aTCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCAATTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTAc                                                                     <  1:782180/141‑1 (MQ=255)
                                                                             cTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACGAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTACTTCGTGTGCTACTCTat                                                                >  2:611650/1‑140 (MQ=255)
                                                                             cTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAg                                                                <  2:579608/141‑1 (MQ=255)
                                                                              taataaTATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATCATCACTTGGTATCTCTTCACCCACCCCTAGTTTTTTCCTGAATTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGc                                                               <  1:472773/141‑1 (MQ=255)
                                                                              taataaTATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGc                                                               <  2:165071/141‑1 (MQ=255)
                                                                                 taataTTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCaaa                                                            <  1:351167/141‑1 (MQ=255)
                                                                                   atatTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGa                                                          <  2:333862/141‑1 (MQ=255)
                                                                                     atTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGaaa                                                        <  2:408451/141‑1 (MQ=255)
                                                                                         cATTATCTAATTTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGt                                                    <  1:562761/141‑1 (MQ=255)
                                                                                              tCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCt                                               >  1:395873/1‑141 (MQ=255)
                                                                                                tAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTCTCtga                                             >  2:72630/1‑141 (MQ=255)
                                                                                                 aaaTTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCtgat                                            <  2:56964/141‑1 (MQ=255)
                                                                                                 aaaTTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCAATTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCtgat                                            <  1:712863/141‑1 (MQ=255)
                                                                                                    tttttGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTgatgat                                         <  1:173638/141‑1 (MQ=255)
                                                                                                    tttttGTTTGAAATTTGAGCATTATAAAATGGATAATCAATTGGTATATCTTCACCAACCCCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTgatgat                                         <  2:49149/141‑1 (MQ=255)
                                                                                                      tttgtttgAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTc                                       >  2:375898/1‑141 (MQ=255)
                                                                                                         gtttgAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTAt                                    >  1:591190/1‑141 (MQ=255)
                                                                                                         gtttgAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTAt                                    <  1:682212/141‑1 (MQ=255)
                                                                                                              aaaTTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCtt                               <  1:729196/141‑1 (MQ=255)
                                                                                                               aaTTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTg                              >  2:420104/1‑141 (MQ=255)
                                                                                                                aTTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTTTTTTTTTTTTTTGCCTAATTCGAGTGCTACTCTATTAAAGAGAATGTTATCTGATGATTCTATTGCTTGt                             >  2:530625/1‑141 (MQ=255)
                                                                                                                  ttGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGttt                           <  1:608303/141‑1 (MQ=255)
                                                                                                                    gAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTa                         >  1:117164/1‑141 (MQ=255)
                                                                                                                      gCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAg                       <  2:566948/141‑1 (MQ=255)
                                                                                                                      gCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAg                       >  1:95276/1‑141 (MQ=255)
                                                                                                                       cATTATAAAATGGATAATCAATTGGTATATCTTCACCAACGCCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGt                      >  2:677665/1‑141 (MQ=255)
                                                                                                                           ataAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGat                  <  1:596486/141‑1 (MQ=255)
                                                                                                                                aTGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTAATGATTCTATTGCTTGTTTTAAGTCGATATTat             >  2:267189/1‑140 (MQ=255)
                                                                                                                                aTGGATAATAACTTGGAAAATCTTCAAAAACACCTAGTTTTTTCAGTCTTTTTTCAAATTTCTTACTGCCTAATTCGTGTTGTACTCTATTAAAGTATATGTTATCTGATGATTCTATTGCTTGTTTTGAGTCGATATTAc             >  2:270331/1‑141 (MQ=255)
                                                                                                                                 tGGATAATCAATTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTAcc            >  1:26956/1‑141 (MQ=255)
                                                                                                                                  ggATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCa           <  1:507082/141‑1 (MQ=255)
                                                                                                                                  ggATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCa           <  1:555954/141‑1 (MQ=255)
                                                                                                                                     tAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCAttt        <  2:19452/141‑1 (MQ=255)
                                                                                                                                      aaTCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTa       >  1:102513/1‑141 (MQ=255)
                                                                                                                                       aTCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTAc      <  2:348265/141‑1 (MQ=255)
                                                                                                                                           cTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTACCACt  <  2:637901/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
ATTTTCTAATGCGCTATAGATTGAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTACCACT  >  CP000730/39514‑39793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: