Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	110,651	C→T	16.8%	D170D (GAC→GAT)	USA300HOU_0109 →	staphylococcal tandem lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	110,651	0	C	T	16.8%	205.0 / 26.2	81	D170D (GAC→GAT)	USA300HOU_0109	staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (31/36);  new base T (4/9);  total (35/46)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01

AAAAGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTC  >  CP000730/110511‑110791                                                                                                                                             |                                                                                                                                              aaaaGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGAc                                                                                                                                               <  1:224421/141‑1 (MQ=255) aaaaGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTCAAAAATTTGAAAAATCATAAAGAc                                                                                                                                               >  2:679151/1‑141 (MQ=255)  aaaGATATCCGATTAAAATGATCGATAATAAAGTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGGCTTTAAAAATTTGAAAAATTATAAAGACg                                                                                                                                              <  1:58774/141‑1 (MQ=255)   aaGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACgg                                                                                                                                             <  1:102500/141‑1 (MQ=255)   aaGATATCCGGTTAAAATGGTCGATAATAAAATCATTCAAACAAAAGAAATTAAAAAAAAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACgg                                                                                                                                             <  2:41708/141‑1 (MQ=255)    aGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGga                                                                                                                                            >  1:519530/1‑141 (MQ=255)    aGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGga                                                                                                                                            <  1:768896/141‑1 (MQ=255)     gATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGgag                                                                                                                                           >  2:456889/1‑141 (MQ=255)         tCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGatat                                                                                                                                       >  2:269273/1‑141 (MQ=255)         tCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGatat                                                                                                                                       >  1:292983/1‑141 (MQ=255)         tCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGatat                                                                                                                                       >  1:462578/1‑141 (MQ=255)         tCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACGCTAAGTTCTTTCTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGatat                                                                                                                                       >  2:176386/1‑141 (MQ=255)           cGGTTAAAATGTACAATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAttt                                                                                                                                     <  2:515156/141‑1 (MQ=255)           cGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTATAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAttt                                                                                                                                     <  1:403990/141‑1 (MQ=255)             gTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCa                                                                                                                                   <  1:385691/141‑1 (MQ=255)                aaaaTGGTCGATAATAAAAGCATAACAACAAAAGAAATGAAAAATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCatat                                                                                                                                <  2:572354/141‑1 (MQ=17)                  aaTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATaa                                                                                                                              >  2:1576/1‑141 (MQ=255)                       tCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCag                                                                                                                         >  2:253245/1‑141 (MQ=255)                             ataaAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGc                                                                                                                   >  1:400770/1‑141 (MQ=255)                              taaAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGcc                                                                                                                  <  1:644033/141‑1 (MQ=255)                              taaAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGcc                                                                                                                  <  1:577686/141‑1 (MQ=255)                              taaAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGGGcc                                                                                                                  >  1:416308/1‑141 (MQ=255)                              taaAATCATTCAAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAAGCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGcc                                                                                                                  <  1:195674/141‑1 (MQ=255)                              aaaaaTCATTCCAACAAAAGAAATGAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGcc                                                                                                                  <  2:172904/140‑1 (MQ=255)                               aaaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCg                                                                                                                 <  1:550589/141‑1 (MQ=255)                                aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGa                                                                                                                <  1:392840/141‑1 (MQ=255)                                  aTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCAAGACGtcactagt                                                                                                              >  2:619941/1‑134 (MQ=255)                                      ttacaaaaaAAGAAAGTAAAAATGAAAAAATAAATAAAGTAATCGAAAACTTTAATTTCTTTGTTCAATATGGCTACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGaataact                                                                                                          <  2:463816/138‑7 (MQ=17)                                        ccAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGaataactcg                                                                                                        <  1:201947/141‑9 (MQ=255)                                           acaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCt                                                                                                     <  1:4254/141‑1 (MQ=255)                                            caaAAGAAATTAAAGAGGAAATAATAAAAAAAGAAATCGAGAGCTGGAAGTTCTTTTTTCAATATGGCGACGTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTa                                                                                                    <  1:747138/141‑1 (MQ=17)                                                   aaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGATCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCa                                                                                             >  2:517901/1‑141 (MQ=255)                                                    aTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACGTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCaa                                                                                            <  2:113530/141‑1 (MQ=255)                                                       aaaGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑a                                                                                        <  2:690661/141‑1 (MQ=255)                                                                aaaaaaaaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATGAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAatgat                                                                               <  2:612104/141‑1 (MQ=255)                                                                aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAatgat                                                                               >  1:507495/1‑141 (MQ=255)                                                                  aaTAAAAAAAGACATCGACAACTTTAAGTTCTTTGTTCAATATGGCGACTTCAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCAAGAGGTGCCGAGTTATACGGCTAAATATCAATT‑AGCTAAtgatga                                                                             >  2:593115/1‑141 (MQ=17)                                                                    aaaaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGAtt                                                                           <  2:449875/140‑1 (MQ=255)                                                                    aaaaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGAtt                                                                           <  2:654961/140‑1 (MQ=255)                                                                     aaaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATta                                                                          <  1:264087/141‑1 (MQ=255)                                                                      aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAACTAATGATGATta                                                                          >  2:161286/1‑141 (MQ=255)                                                                      aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATtat                                                                         >  1:166858/1‑141 (MQ=255)                                                                       aaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATA‑AACTAATGATGATtata                                                                        <  2:221008/141‑1 (MQ=255)                                                                              aaTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTaaa                                                                 <  2:259833/141‑1 (MQ=255)                                                                                 cGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACa                                                              >  2:323781/1‑141 (MQ=255)                                                                                   aaaaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAAt                                                            <  1:1576/141‑1 (MQ=255)                                                                                       cTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACg                                                        <  1:41547/141‑1 (MQ=255)                                                                                         ttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCa                                                      >  2:485153/1‑141 (MQ=255)                                                                                           aaGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCaaa                                                    >  2:438720/1‑141 (MQ=255)                                                                                           aaGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCaaa                                                    >  2:294116/1‑141 (MQ=255)                                                                                            aGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCaaaa                                                   >  2:142404/1‑141 (MQ=255)                                                                                            aGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCaaaa                                                   >  1:435813/1‑141 (MQ=255)                                                                                                     ttCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgata                                          >  2:577961/1‑141 (MQ=255)                                                                                                         atatGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAcc                                      <  1:456889/141‑1 (MQ=255)                                                                                                          tatGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcg                                     >  2:412351/1‑141 (MQ=255)                                                                                                          tatGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcg                                     >  1:461861/1‑141 (MQ=255)                                                                                                             ggCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacg                                  <  2:99242/141‑1 (MQ=255)                                                                                                              gCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacga                                 >  1:701459/1‑141 (MQ=255)                                                                                                              gCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacga                                 >  1:283658/1‑141 (MQ=255)                                                                                                                  ccttAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                             <  2:229314/139‑1 (MQ=255)                                                                                                                  cTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATAGAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAACTACGCAAAAAATATGATATACCGACCAGTaa                             >  2:319529/1‑141 (MQ=255)                                                                                                                     taaagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGc                          >  2:482109/14‑141 (MQ=11)                                                                                                                      aaagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCt                         >  2:437547/13‑141 (MQ=11)                                                                                                                       aagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTc                        <  1:698183/130‑1 (MQ=14)                                                                                                                        agatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                       <  2:201501/131‑1 (MQ=14)                                                                                                                        aaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                       >  2:68584/1‑141 (MQ=255)                                                                                                                        aaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                       <  2:755663/141‑1 (MQ=255)                                                                                                                          atttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCTAAAGATATGATATACCGACGAGTAAAGCTCCaa                     <  1:409579/133‑1 (MQ=14)                                                                                                                          aTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTTTAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaa                     <  1:400186/141‑1 (MQ=255)                                                                                                                          aTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACACTTAAGCACAACATATGATATACCGACGAGTAAAGCTCCaa                     <  2:234572/141‑1 (MQ=255)                                                                                                                          aTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaa                     >  2:635796/1‑141 (MQ=255)                                                                                                                           tttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTGTTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaaa                    <  1:550221/134‑1 (MQ=14)                                                                                                                           tttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaaa                    >  1:84389/8‑141 (MQ=14)                                                                                                                           tttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaaa                    >  2:29466/8‑141 (MQ=14)                                                                                                                             tGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGt                  >  1:221570/1‑141 (MQ=255)                                                                                                                              aatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATGATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGtt                 <  2:686768/137‑1 (MQ=17)                                                                                                                                 gagtgataAAGAGGGTGATATATCATATAATCCAGAGGTGCCGAGTTATGCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAtt              <  2:27488/136‑1 (MQ=17)                                                                                                                                   aTTATAAAGATTGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGt            <  2:408405/141‑1 (MQ=255)                                                                                                                                    ttATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGtt           <  1:153436/141‑1 (MQ=255)                                                                                                                                     tataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAACACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTa          <  1:377849/141‑1 (MQ=255)                                                                                                                                      ataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaa         <  2:138031/141‑1 (MQ=255)                                                                                                                                      ataAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaa         <  2:599882/141‑1 (MQ=255)                                                                                                                                         aaGACGGAGATATTTCAGATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAg      <  2:508761/141‑1 (MQ=255)                                                                                                                                             cGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTc  <  2:248429/141‑1 (MQ=255)                                                                                                                                             |                                                                                                                                              AAAAGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATT‑AACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTC  >  CP000730/110511‑110791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑