Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 396,540 G→A 87.8% A208T (GCA→ACA)  USA300HOU_0364 → possible lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730396,5400GA87.8% 217.4 / 17.0 82A208T (GCA→ACA) USA300HOU_0364possible lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (7/3);  new base A (47/24);  total (55/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

ACATTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAGT  >  CP000730/396402‑396675
                                                                                                                                           |                                                                                                                                        
aCATTAGATATCATACCAAAATTAATGTTAAAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGTAGAAGATATTTATTCACATACAGATTTATATGATTTT‑AAAGCTATCGTTGAAGGcaca                                                                                                                                        <  2:212337/141‑1 (MQ=255)
  aTTAGATATCATACCAAAATTAATGTTACTAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGcacaca                                                                                                                                      <  1:497604/141‑1 (MQ=255)
   ttAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AGAGCGAACGTTGAAGGCACACaa                                                                                                                                     >  2:555857/1‑141 (MQ=255)
     aGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCGCACaaaa                                                                                                                                   <  1:9267/141‑1 (MQ=255)
       atatCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACaaaaaa                                                                                                                                 >  2:671062/1‑141 (MQ=255)
          tCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAAttt                                                                                                                              <  1:518620/141‑1 (MQ=255)
            atgccAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGCACGTTGAAGGCGCACAAAAAATTTAt                                                                                                                            >  1:625223/4‑141 (MQ=255)
                   aaTTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATGTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTAt                                                                                                                     >  2:751032/1‑141 (MQ=255)
                     ttAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTAttt                                                                                                                   >  2:361769/1‑141 (MQ=255)
                     ttAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTAttt                                                                                                                   >  1:93185/1‑141 (MQ=255)
                      tAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTa                                                                                                                  <  1:69723/141‑1 (MQ=255)
                        aTGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTaaa                                                                                                                >  1:344972/1‑141 (MQ=255)
                         tGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAAc                                                                                                               >  2:572356/1‑141 (MQ=255)
                          gTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAAc                                                                                                               <  2:467553/141‑1 (MQ=255)
                             aCAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTAt                                                                                                           >  1:719434/1‑141 (MQ=255)
                                   ttCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTaga                                                                                                     <  1:154237/141‑1 (MQ=255)
                                     cTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAgaga                                                                                                   >  1:558481/1‑141 (MQ=255)
                                      tGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaa                                                                                                  >  1:161675/1‑141 (MQ=255)
                                        ttGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaaaa                                                                                                <  1:2065/141‑1 (MQ=255)
                                          gACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaaaaaa                                                                                              >  1:583558/1‑141 (MQ=255)
                                            ccTATTAAATGAAGTTCATACGGCTAAAATCACAGGTGAAGAAGAAATTTATTCATATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTTAACCTATTTTAGAGAAAAAAGa                                                                                            <  1:6845/141‑1 (MQ=255)
                                              tATTAAATGAAGTTGCAACTTCTAAAATAACCGGTGAAGAAGAAATTTCTTCACATACCGATTTATATGACTTT‑CCAGCGACCGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑a                                                                                         <  1:589389/141‑1 (MQ=255)
                                               aTTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑aa                                                                                        <  1:448434/141‑1 (MQ=255)
                                                  aaaTGAAGTTGAAACTTATAAAAAAACAGGAGAAGAAGAAATTTATTCAAATACAGATTTATATGATTTT‑AAAGAGAACGTTAAAAGCAAAAAAAAAATTTATGACTTATTTAAACCTATTATAGAAAAAAAAGAT‑aaaaa                                                                                     <  2:377805/141‑1 (MQ=255)
                                                       aaGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTaa                                                                                >  2:189621/1‑141 (MQ=255)
                                                        aGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAg                                                                               >  1:516217/1‑141 (MQ=255)
                                                         gTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGt                                                                              >  1:647154/1‑141 (MQ=255)
                                                         gTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaa                                                                                                  >  2:74945/1‑122 (MQ=255)
                                                         gTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaa                                                                                                  <  1:74945/122‑1 (MQ=255)
                                                         gTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGt                                                                              >  1:132411/1‑141 (MQ=255)
                                                          ttGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGtg                                                                             <  1:606982/141‑1 (MQ=255)
                                                              aaCTTCTAAAATCACAGGTGAAGAAGAAATTTATTGACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AGAAAATTAAGtgatga                                                                         >  1:651456/1‑141 (MQ=255)
                                                              aaCTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGtgatga                                                                         >  1:194820/1‑141 (MQ=255)
                                                               aCTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTgatgat                                                                        >  2:100052/1‑141 (MQ=255)
                                                               aCTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTgatgat                                                                        >  1:782842/1‑141 (MQ=255)
                                                                   cTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATcc                                                                    >  1:562922/1‑141 (MQ=255)
                                                                   cTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTCAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATcc                                                                    >  2:658980/1‑141 (MQ=255)
                                                                     aaaaTCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCaa                                                                  >  2:683678/1‑141 (MQ=255)
                                                                      aaaTCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCaaa                                                                 >  1:165133/1‑141 (MQ=255)
                                                                      aaaTCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCaaa                                                                 >  2:305108/1‑141 (MQ=255)
                                                                      aaaTCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAACGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCaaa                                                                 >  1:594779/1‑141 (MQ=255)
                                                                       aaTCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAAt                                                                >  1:544323/1‑141 (MQ=255)
                                                                         acacaGGTGAAAAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACAAAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAAATTAAGTGATGATATCCAAATg                                                               <  1:517817/140‑1 (MQ=255)
                                                                           acaGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAAc                                                            >  1:70716/1‑141 (MQ=255)
                                                                            caGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACt                                                           >  1:346199/1‑141 (MQ=255)
                                                                               gTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCg                                                        >  2:632423/1‑141 (MQ=255)
                                                                                 gaagaagaaATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGAt                                                      >  1:390903/1‑141 (MQ=255)
                                                                                    gaagaaATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATaaa                                                   >  1:728540/1‑141 (MQ=255)
                                                                                       gaaATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTg                                                <  2:731706/141‑1 (MQ=255)
                                                                                         gaTTTATACACATACAGATGTATATGATTTT‑AAAGCGACCGTTGAAGGCACAGAAAATATTTATGACTTATTTAACCCTATATTAGAGAAAAAAAAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGaa                                              <  2:295850/140‑1 (MQ=255)
                                                                                         aaTTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGaa                                              <  2:90248/141‑1 (MQ=255)
                                                                                         aaTTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGaa                                              >  2:252436/1‑141 (MQ=255)
                                                                                         aaTTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGaa                                              <  2:592250/141‑1 (MQ=255)
                                                                                           tttATTCACAAACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATc                                            >  2:406995/1‑141 (MQ=255)
                                                                                               ttCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAatt                                        <  1:100052/141‑1 (MQ=255)
                                                                                                     aaCAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑CAAAAATTAAGTGATGATATCCAACTGAACTTCGATAAAGTGAATCAATTATTGGa                                  <  1:704428/140‑1 (MQ=255)
                                                                                                       cAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATa                                <  1:189702/141‑1 (MQ=255)
                                                                                                           tttATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAata                            >  2:220871/1‑141 (MQ=255)
                                                                                                           tttATATGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAata                            >  2:782343/1‑141 (MQ=255)
                                                                                                             tataTGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAAtata                          <  1:531667/141‑1 (MQ=255)
                                                                                                              atatGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATaa                         <  1:530773/141‑1 (MQ=255)
                                                                                                               tatGATTTT‑AAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATaaa                        >  2:488426/1‑141 (MQ=255)
                                                                                                               tatGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATaaa                        >  1:701730/1‑141 (MQ=255)
                                                                                                                atGATTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAg                       >  1:20286/1‑141 (MQ=255)
                                                                                                                 tGATTTT‑AAAGCGAACGTTGAACGCCCACAAAAAATTTATGACTTCTTTCAACCTCTCTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATaaaca                      >  2:504708/1‑139 (MQ=255)
                                                                                                                   aTTTT‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATa                    <  2:681013/141‑1 (MQ=255)
                                                                                                                       t‑AAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGGGAATCAATTATTGGATAAATATAAAGATaacaa                <  1:647578/141‑1 (MQ=255)
                                                                                                                         aaaGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATaacaac               >  2:638408/1‑141 (MQ=255)
                                                                                                                         aaaGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATaacaac               >  2:382972/1‑141 (MQ=255)
                                                                                                                            gCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcggc            >  2:329437/1‑141 (MQ=255)
                                                                                                                              gAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcggcgg          >  2:251501/1‑141 (MQ=255)
                                                                                                                                aCGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGtt        >  2:161211/1‑141 (MQ=255)
                                                                                                                                aCGTTGAAGGCACACAAAAAATTTATGACTTATTCAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACtt                                                          <  2:372312/91‑1 (MQ=255)
                                                                                                                                aCGTTGAAGGCACACAAAAAATTTATGACTTATTCAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACtt                                                          >  1:372312/1‑91 (MQ=255)
                                                                                                                                aCGTTGAAGGCACACAAAAAATTTATGACTTATTCAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACtt                                                          >  1:372258/1‑91 (MQ=255)
                                                                                                                                aCGTTGAAGGCACACAAAAAATTTATGACTTATTCAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACtt                                                          <  2:372258/91‑1 (MQ=255)
                                                                                                                                  gTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATATTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATAATTGGATAAATATAAAGATAACAACGGCGGTTAt      >  1:446457/1‑141 (MQ=255)
                                                                                                                                   ttGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATg     >  1:457365/1‑141 (MQ=255)
                                                                                                                                   ttGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATg     >  1:681291/1‑141 (MQ=255)
                                                                                                                                    tGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGa    >  1:764029/1‑141 (MQ=255)
                                                                                                                                     gAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAg   >  1:327464/1‑141 (MQ=255)
                                                                                                                                     gAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAg   >  1:446657/1‑141 (MQ=255)
                                                                                                                                      aaGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAGt  >  1:44276/1‑141 (MQ=255)
                                                                                                                                      aaGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAGt  <  1:751032/141‑1 (MQ=255)
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ACATTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTT‑AAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAGT  >  CP000730/396402‑396675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑