Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	575,701	T→C	5.9%	I193I (ATT→ATC)	gltX →	glutamate‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	575,701	0	T	C	5.9%	210.7 / 2.1	69	I193I (ATT→ATC)	gltX	glutamate‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (36/28);  new base C (2/2);  total (39/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

AAGCAGAAGGACGCCAACCATCAATTCGTTTCCGAGTACCTCAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCTAAACAAATTATGATTTATGAAGCATTTGGCTGGGAGCCACCTCGTTT  >  CP000730/575562‑575838                                                                                                                                            |                                                                                                                                          aaGCAGAAGGACGCCAACCATCAATTCGTTTCCGAGTACCTCAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTc                                                                                                                                          >  1:130543/1‑141 (MQ=255)      gAAGGACGCCAACCATCAATTCGTTTCCGAGTACCTCAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACg                                                                                                                                     <  2:547614/141‑1 (MQ=255)       aaGGACGCCAACCATCAATTCGTTTCCGAGTACCTCAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGt                                                                                                                                    <  1:228511/141‑1 (MQ=255)            cGCCAACCATCAATTCGTTTCCGAGTACCTCAAAACCAAACGTATTCATTTGTTGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATCCCAACGTACAAt                                                                                                                               >  2:778603/1‑141 (MQ=255)            cGCCAACCATCAATTCGTTTCCGAGTACCTCAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAAt                                                                                                                               <  1:87775/141‑1 (MQ=255)            cGCCAACCATCAATTCGTTTCCGAGTACCTCAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATCCCAACGTACAAt                                                                                                                               >  2:778655/1‑141 (MQ=255)               cAACCATCAATTCGTTTCCGAGTACCTCAAAACCAAACGTATTCATTTGATGATATGGTAAATGGAAATATTTCATTTGATTCAAATGGTATTAGTGACGGGGTTATTGTAAAAAAAGATGGCATACCAACGTACAAattt                                                                                                                            >  1:290445/1‑141 (MQ=255)                 aCCATCAATTCGTTTCCGAGTACCTCAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGc                                                                                                                          >  2:564706/1‑141 (MQ=255)                 aCCATCAATTCGTTTCCGAGTACCTCAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGAGTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGc                                                                                                                          <  2:126147/141‑1 (MQ=255)                      cAACTCGTTTCCGAGTACCTCAAAACCAAACGTATTCATTTGATGACCTGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAg                                                                                                                     >  2:656835/1‑141 (MQ=255)                        aTTCGTTTCCGAGTACCTCAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCt                                                                                                                   >  1:399620/1‑141 (MQ=255)                        aTTCGTTTCCGAGTACCTCAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGAGGGCATTCCAACGTACAATTTTGCAGTAGCt                                                                                                                   >  1:50116/1‑141 (MQ=255)                         ttCGTTTCCGAGTACCTCAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCta                                                                                                                  >  2:31223/1‑141 (MQ=255)                            gTTTCCGAGTACCTCAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAg                                                                                                               >  2:459878/1‑141 (MQ=255)                             tttCCGAGTACCTCAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAga                                                                                                              >  1:40140/1‑141 (MQ=255)                              ttCCGAGTACCTCAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGAATGAGATATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAgat                                                                                                             >  2:243985/1‑141 (MQ=255)                                 cGAGTACCTCAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACg                                                                                                                                     <  2:338279/114‑1 (MQ=255)                                 cGAGTACCTCAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACg                                                                                                                                     >  1:338279/1‑114 (MQ=255)                                         tCAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACAt                                                                                                  >  2:742742/1‑141 (MQ=255)                                           aaaaCCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGc                                                                                                <  2:127726/141‑1 (MQ=255)                                            aaaCCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCa                                                                                               <  1:535673/141‑1 (MQ=255)                                              aCCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCaaa                                                                                             >  2:261159/1‑141 (MQ=255)                                                  aaCGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTc                                                                                         >  1:12671/1‑141 (MQ=255)                                                     gTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGa                                                                                      <  1:698436/141‑1 (MQ=255)                                                       aTTCATTTGATGATATGGTAAAAGGAAATATTTCAGTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATg                                                                                    <  1:72570/141‑1 (MQ=255)                                                        ttCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGt                                                                                   <  2:667723/141‑1 (MQ=255)                                                         tCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTa                                                                                  >  2:218654/1‑141 (MQ=255)                                                          cATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTaa                                                                                 >  2:565177/1‑141 (MQ=255)                                                           aTTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAAt                                                                                <  2:409391/141‑1 (MQ=255)                                                           aTTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAAt                                                                                >  1:479313/1‑141 (MQ=255)                                                           aTTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAAt                                                                                <  1:204874/141‑1 (MQ=255)                                                           aTTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAAt                                                                                <  1:86770/141‑1 (MQ=255)                                                              tgatgaTATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCg                                                                             >  2:143816/1‑141 (MQ=255)                                                              tgatgaTATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCg                                                                             <  1:688563/141‑1 (MQ=255)                                                                   atatGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCgtggtg                                                                        <  1:552788/141‑1 (MQ=255)                                                                          aaaaGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCa                                                                 <  1:236390/141‑1 (MQ=255)                                                                            aaGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCata                                                               <  2:207908/141‑1 (MQ=255)                                                                             aGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGAGCatat                                                              >  1:657345/1‑141 (MQ=255)                                                                               gAAATATTTAATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACTTTCACCTTTCAGATGTAATTCGTGGTGATGATCATAttt                                                            <  2:668356/141‑1 (MQ=255)                                                                                aaaTATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTc                                                           >  2:615503/1‑141 (MQ=255)                                                                                 aaTATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCa                                                          >  2:88944/1‑141 (MQ=255)                                                                                  atatTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCaa                                                         <  1:752535/141‑1 (MQ=255)                                                                                       tCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACg                                                    >  2:712783/1‑141 (MQ=255)                                                                                       tCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACg                                                    >  1:269710/1‑141 (MQ=255)                                                                                        cATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATCCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGc                                                   <  1:778655/141‑1 (MQ=255)                                                                                        cATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATCCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGc                                                   <  1:778603/141‑1 (MQ=255)                                                                                         aTTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGcc                                                  >  1:137291/1‑141 (MQ=255)                                                                                          tttGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCt                                                 <  1:576104/141‑1 (MQ=255)                                                                                              aTTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCTAAAc                                             >  1:290477/1‑141 (MQ=255)                                                                                                 cAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCTAAACaaa                                          <  2:50116/141‑1 (MQ=255)                                                                                                 cAAATGGTATTGGTGACTGGGTTATCGT‑AAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCTAAACAAAt                                         >  1:4347/1‑141 (MQ=255)                                                                                                  aaaTGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCTAAACAAAt                                         >  2:148764/1‑141 (MQ=255)                                                                                                   aaTGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCTAAACAAAtt                                        <  1:767218/141‑1 (MQ=255)                                                                                                   aaTGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCTAAACAAAtt                                        >  1:528075/1‑141 (MQ=255)                                                                                                    aTGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCTAAACAAATTa                                       <  2:533498/141‑1 (MQ=255)                                                                                                    aTGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCTAAACAAATTa                                       <  2:533431/141‑1 (MQ=255)                                                                                                      ggTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCTAAACAAATTATg                                     <  2:560121/141‑1 (MQ=255)                                                                                                           tGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCTAAACAAATTATGATTTa                                >  1:435136/1‑141 (MQ=255)                                                                                                               gACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGGGATGATCATATTTCAAACACGCCTAAACAAATTATGATTTATGaa                            >  2:539216/1‑141 (MQ=255)                                                                                                                   gggTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCTAAACAAATTATGATTTATGAAGCAt                        >  1:655887/1‑141 (MQ=255)                                                                                                                                aaaaGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCTAAACAAATTATGATTTATGAAGCATTTGGCTGGGAGcc           <  1:32099/141‑1 (MQ=255)                                                                                                                                aaaaGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCTAAACAAATTATGATTTATGAAGCATTTGGCTGGGAGcc           <  2:50335/141‑1 (MQ=255)                                                                                                                                aaaaGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCTAAACAAATTATGATTTATGAAGCATTTGGCTGGGAGcc           <  2:573177/141‑1 (MQ=255)                                                                                                                                    gATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCTAAACAAATTATGATTTATGAAGCATTTGGCTGGGAGCCACCt       >  2:580097/1‑141 (MQ=255)                                                                                                                                     aTGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCTAAACAAATTATGATTTATGAAGCATTTGGCTGGGAGCCACCTc      >  2:614206/1‑141 (MQ=255)                                                                                                                                     aTGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCTAAACAAATTATGATTTATGAAGCATTTGGCTGGGAGCCACCTc      >  2:448593/1‑141 (MQ=255)                                                                                                                                       ggCATTCCAACGTACAATTTTGCAGTAGCTATAGATGACCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCTAAACAAATTATGATTTATGAAGCATTTGGCTGGGAGCCACCTCGt    >  1:80167/1‑141 (MQ=255)                                                                                                                                         ccttCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCTAAACAAACTATGATTTATGAAACATTTGCCTGGGAGCCACCTCGttt  >  2:636210/3‑141 (MQ=255)                                                                                                                                         cATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCTAAACAAATTATGATTTATGAAGCATTTGGCTGGGAGCCACCTCGttt  >  2:311/1‑141 (MQ=255)                                                                                                                                         cATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCTAAACAAATTATGATTTATGAAGCATTTGGCTGGGAGCCACCTCGttt  >  2:277861/1‑141 (MQ=255)                                                                                                                                            |                                                                                                                                          AAGCAGAAGGACGCCAACCATCAATTCGTTTCCGAGTACCTCAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCAAACACGCCTAAACAAATTATGATTTATGAAGCATTTGGCTGGGAGCCACCTCGTTT  >  CP000730/575562‑575838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑