Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 596,093 T→G 6.7% intergenic (+1247/‑1174) rpsG → / → tufA ribosomal protein S7/elongation factor EF1A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730596,0930TG 6.7% 256.5 / 9.2 87intergenic (+1247/‑1174)rpsG/tufAribosomal protein S7/elongation factor EF1A
Reads supporting (aligned to +/- strand):  ref base T (38/43);  new base G (2/4);  total (40/47)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

GTGTGTATTCAGGTACAATGACATCTGGTTCATACGTTAAGAACTCTACTAAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCAAAATCTAAAGCTGACCAAGAT  >  CP000730/595954‑596231
                                                                                                                                           |                                                                                                                                          
gtgtgtATTCAGGTACAATGACATCTGGTTCATACGTTAAGAACTCTGCTAAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCgctg                                                                                                                                           <  2:31605/141‑1 (MQ=255)
 tgtgtATTCAGGTACAATGACATCTGGTTCATACGTTAAGAACTCTACTAAAGGTAAACGTGAACGTGTAGGTCGTTTATGACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCgctgc                                                                                                                                          >  2:78124/1‑141 (MQ=255)
    gtATTCAGGTACAATGACATCTGGTTCATACGTTAAGAACTCTACTAAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCcgctgc                                                                                                                                       >  2:71637/1‑141 (MQ=255)
     tATTCAGGTACAATGACATCTGGTTCATACGTTAAGAACTCTACTAAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCg                                                                                                                                      >  1:781127/1‑141 (MQ=255)
         cAGGTACAATGACATCTGGTTCATACGTTAAGAACTCTACTAAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAg                                                                                                                                  >  1:463277/1‑141 (MQ=255)
         cAGGTACAATGACATCTGGTTCATACGTTAAGAACTCTACTAAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAg                                                                                                                                  >  1:594792/1‑141 (MQ=255)
             tACAATGACATCTGGTTCATACGTTAAGAACTCTACTAAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCt                                                                                                                              <  2:192259/141‑1 (MQ=255)
              aCAATGACATCTGGTTCATACGTTAAGAACTCTACTAAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCtt                                                                                                                             >  1:198438/1‑141 (MQ=255)
                       tCTGGTTCATACGTTAAGAACTCTACTAAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACa                                                                                                                    <  1:573070/141‑1 (MQ=255)
                         tGGTTCATACGTTAAGAACTCTACTAAAGGTAACCGTGAAAGGGTAGGTGGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCGAGAGATACCGTTGCAGCAGAGGGTCTTAAAGATTCAgg                                                                                                                  >  2:378133/1‑141 (MQ=255)
                           gTTCATACGTTAAGAACTCTACTAAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTa                                                                                                                >  2:419877/1‑141 (MQ=255)
                               aTACGTTAAGAACTCTACTAAAGGTAAACGTGAACGTGTCGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTgg                                                                                                            <  1:581485/141‑1 (MQ=255)
                               aTACGTTAAGAACTCTACTAAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTgg                                                                                                            >  2:466893/1‑141 (MQ=255)
                                tACGTTAAGAGCTCTACTAAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGt                                                                                                           <  1:614477/141‑1 (MQ=255)
                                  cGTTAAGAACTCTACTAAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGa                                                                                                         >  2:674841/1‑141 (MQ=255)
                                     tAAGAACTCTACTAAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATAc                                                                                                      <  1:419877/141‑1 (MQ=255)
                                     tAAGAACTCTACTAAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATAc                                                                                                      >  2:24325/1‑141 (MQ=255)
                                      aaGAACTCTACTAAAGGTAAACGTGAACGTGTAGGTCGGTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGCTACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACt                                                                                                     >  2:184226/1‑141 (MQ=255)
                                        gcaCTCTACTAAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACctt                                                                                                   <  2:74446/139‑1 (MQ=255)
                                         aaCTCTACTAAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTa                                                                                                  >  1:273720/1‑141 (MQ=255)
                                         aaCTCTACTAAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTa                                                                                                  >  1:378169/1‑141 (MQ=255)
                                            tctACTAAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTAtgt                                                                                               >  2:232565/1‑141 (MQ=255)
                                             ctactaAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTAtgtg                                                                                              >  1:211524/1‑141 (MQ=255)
                                             ctactaAAGCTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTAtgtg                                                                                              >  1:690894/1‑141 (MQ=255)
                                              tactaAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATgtgg                                                                                             <  1:596548/141‑1 (MQ=255)
                                                  aaaGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTgag                                                                                         <  2:326094/141‑1 (MQ=255)
                                                  aaaGGTAAACGTGAACGTGTAGATCGTTTAGTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTgag                                                                                         >  2:262363/1‑141 (MQ=255)
                                                    aGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCGGCTGCGGTAGGTCTTAAAGATAc                                                                                                                     >  2:161433/1‑111 (MQ=255)
                                                    aGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCGGCTGCGGTAGGTCTTAAAGATAc                                                                                                                     <  1:161433/111‑1 (MQ=255)
                                                     ggTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGaaa                                                                                      >  2:412455/1‑141 (MQ=255)
                                                       tAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGaaaaa                                                                                    >  2:322926/1‑141 (MQ=255)
                                                        aaaCGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAAt                                                                                   <  2:304590/141‑1 (MQ=255)
                                                         aacgtgaacgtgTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATg                                                                                  <  1:2740/141‑1 (MQ=255)
                                                           cgtgaacgtgTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGAc                                                                                <  2:168242/141‑1 (MQ=255)
                                                           cgtgaacgtgTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGAc                                                                                <  1:254769/141‑1 (MQ=255)
                                                            gtgaacgtgTAGGTCGTTTATTACAAATTCACGCTAACTCTCGGCAAGAAAGCGATACTGTAGACTCGGTAGATATGGCGGCGGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACa                                                                               >  1:115812/1‑141 (MQ=255)
                                                             tgaacgtgTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACAt                                                                              <  2:264136/141‑1 (MQ=255)
                                                             tgaacgtgTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACAt                                                                              <  2:411989/141‑1 (MQ=255)
                                                             tgaacgtgTAGGGCGTTTATTACAAATGCACGCGAACTCGCGTCAAGAAATCGATACTGTATCCTCTGGAGATATAGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGGAAAATGACAt                                                                              <  2:166979/141‑1 (MQ=255)
                                                              gaacgtgTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACAtt                                                                             <  2:77282/141‑1 (MQ=255)
                                                                acgtgTAGGTCGTGTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTAt                                                                           >  2:433574/1‑141 (MQ=255)
                                                                 cgtgTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATc                                                                          >  1:351692/1‑141 (MQ=255)
                                                                   tgtAGGTCGTTTATTACAAAAGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTAAAAAAAAAAAAAAAATGACATTATCtt                                                                        <  2:398924/141‑1 (MQ=255)
                                                                    gtAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTg                                                                       <  2:224239/141‑1 (MQ=255)
                                                                           gTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCaa                                                                <  1:274206/141‑1 (MQ=255)
                                                                            tttATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACCTTACCTGGTGAGAAAAATGACATTATCTTGGAATCAAt                                                               <  2:234363/141‑1 (MQ=255)
                                                                             ttattaCGAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAGATGACATTATCTTGGAATCAATg                                                              >  1:623291/1‑141 (MQ=255)
                                                                             ttattaCAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATg                                                              <  2:164427/141‑1 (MQ=255)
                                                                               attaCAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGa                                                            <  1:315931/141‑1 (MQ=255)
                                                                               attaCAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGa                                                            <  2:375244/141‑1 (MQ=255)
                                                                                 taCAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAAt                                                          <  1:601407/141‑1 (MQ=255)
                                                                                     aaTGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTccc                                                      >  1:39167/1‑141 (MQ=255)
                                                                                          aCGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGc                                                 <  1:100012/141‑1 (MQ=255)
                                                                                           cGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGcc                                                >  1:111437/1‑141 (MQ=255)
                                                                                           cGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGcc                                                >  2:458428/1‑141 (MQ=255)
                                                                                            gCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCGGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCa                                               <  1:108782/141‑1 (MQ=255)
                                                                                            gCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCGGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCa                                               <  1:502578/141‑1 (MQ=255)
                                                                                              tAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGt                                             <  2:182325/141‑1 (MQ=255)
                                                                                               aaCTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGtt                                            <  1:232565/141‑1 (MQ=255)
                                                                                                aCTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTa                                           <  2:781127/141‑1 (MQ=255)
                                                                                                aCTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGAAATCAATGGAATTCCCAGAGCCAGTTa                                           >  1:609798/1‑141 (MQ=255)
                                                                                                   cACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTc                                        >  1:285945/1‑141 (MQ=255)
                                                                                                   cACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTc                                        >  1:655694/1‑141 (MQ=255)
                                                                                                       tCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACtt                                    <  1:292401/141‑1 (MQ=255)
                                                                                                         aaGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTAt                                  >  2:625560/1‑141 (MQ=255)
                                                                                                           gAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCa                                >  2:145939/1‑141 (MQ=255)
                                                                                                               tCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTag                            <  2:245785/141‑1 (MQ=255)
                                                                                                                   tACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTAAAAAAAAAAAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGcc                        <  2:273720/141‑1 (MQ=255)
                                                                                                                   tACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCCTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGcc                        <  1:182740/141‑1 (MQ=255)
                                                                                                                       gTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGATAAAAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCaaaa                    <  2:710912/141‑1 (MQ=255)
                                                                                                                        tataCGCGGGAGAGAGCGCGTCGGCGGGATGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGATCCAGTTATTCACTTATCAGTAGAGCCAAAAt                   <  2:781865/141‑1 (MQ=255)
                                                                                                                         ataCTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCAAAATc                  <  2:174788/141‑1 (MQ=255)
                                                                                                                         aatccctGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCAAAATc                  <  1:701438/136‑1 (MQ=255)
                                                                                                                          taCTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCAAAATCt                 >  1:5833/1‑141 (MQ=255)
                                                                                                                          taCTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCAAAATCt                 >  2:335445/1‑141 (MQ=255)
                                                                                                                           aCTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCAAAATCTa                >  2:37077/1‑141 (MQ=255)
                                                                                                                           aCTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCAAAATCTa                <  2:229600/141‑1 (MQ=255)
                                                                                                                               tGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACt                                                                                                     >  1:196659/1‑52 (MQ=255)
                                                                                                                               tGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACt                                                                                                     <  2:196659/52‑1 (MQ=255)
                                                                                                                               tGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCAAAATCTAAAGc            <  1:92255/141‑1 (MQ=255)
                                                                                                                               tGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCAAAATCTAAAGc            <  1:230622/141‑1 (MQ=255)
                                                                                                                               gggAGATATCGCTGCCGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCAAAATCTAAAGc            <  1:149079/140‑1 (MQ=255)
                                                                                                                                 gagaTATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCAAAATCTAAAGCTg          >  1:376094/1‑141 (MQ=255)
                                                                                                                                     tatCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAGATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCAAAATCTAAAGCTGACCa      <  2:623291/141‑1 (MQ=255)
                                                                                                                                      atCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCAAAATCTAAAGCTGACCaa     >  2:492956/1‑141 (MQ=255)
                                                                                                                                      atCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCAAAATCTAAAGCTGACCaa     >  2:651809/1‑141 (MQ=255)
                                                                                                                                      atCGCTGCTGCGGCAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCAAAATCTAAAGCTGACCaa     >  2:769495/1‑141 (MQ=255)
                                                                                                                                       tCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCAAAATCTAAAGCTGACCAAg    <  2:60548/141‑1 (MQ=255)
                                                                                                                                       tCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCAAAATCTAAAGCTGACCAAg    <  1:620874/141‑1 (MQ=255)
                                                                                                                                        cGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCAAAATCTAAAGCTGACCAAGa   >  2:237917/1‑141 (MQ=255)
                                                                                                                                         gctgctGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCAAAATCTAAAGCTGACCAAGAt  >  2:364776/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GTGTGTATTCAGGTACAATGACATCTGGTTCATACGTTAAGAACTCTACTAAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCTAACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTTAAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCAATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCAAAATCTAAAGCTGACCAAGAT  >  CP000730/595954‑596231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑