Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	849,656	G→A	9.3%	G261D (GGT→GAT)	yvcJ →	ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	849,656	0	G	A	9.3%	222.0 / 8.7	75	G261D (GGT→GAT)	yvcJ	ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (27/41);  new base A (2/5);  total (29/46)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

ATTAGATAAAGACGTTTATAATTATGTTATGAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGAAAGTGGCGAGAAAAAATGAGACAAA  >  CP000730/849519‑849792                                                                                                                                          |                                                                                                                                         aTTAGATAAAGACGTTTATAATTATGTTATGAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTg                                                                                                                                       >  2:308696/1‑141 (MQ=255)  ttAGATAAAGACGTTTATAATTATGTTATGAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGt                                                                                                                                      <  2:254597/141‑1 (MQ=255)  ttAGATAAAGACGTTTATAATTATGTTATGAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGt                                                                                                                                      <  2:486669/141‑1 (MQ=255)      aTAAAGACGTTTATAATTATGTTATGAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGATTGTACgg                                                                                                                                  <  1:220306/141‑1 (MQ=255)       tAAAGACGTTTATAATTATGTTATGAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACggg                                                                                                                                 >  2:118344/1‑141 (MQ=255)       tAAAGACGTTTATAATTATGTTATGAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACggg                                                                                                                                 <  1:335864/141‑1 (MQ=255)           aaggTTTATAATTATGATATGAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTTATTTGTTAGATTATATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGa                                                                                                                             <  2:81816/138‑1 (MQ=255)            aCGTTTATAATTATGTTATGAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGac                                                                                                                            <  1:593942/141‑1 (MQ=255)                ttATAATTATGTTATGAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGacaaca                                                                                                                        <  1:513762/141‑1 (MQ=255)                  ataATTATGTTATGAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATc                                                                                                                      <  1:16499/141‑1 (MQ=255)                   taATTATGTTATGAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTGGACAACATCg                                                                                                                     >  1:465048/1‑141 (MQ=255)                    aaTTATGTTATGAAATGGAAAGAGACGGAGATTTTCTTGGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGa                                                                                                                    >  1:416397/1‑141 (MQ=255)                     aTTATGTTATGAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGAt                                                                                                                   <  2:200710/141‑1 (MQ=255)                       tatgttatgAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCt                                                                                                                 <  1:531031/141‑1 (MQ=255)                        atgttatgAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTg                                                                                                                >  1:215054/1‑141 (MQ=255)                        atgttatgAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTGGACAACATCGATCTg                                                                                                                <  2:416085/141‑1 (MQ=255)                         tgttatgAAATGGAAAGAGACTGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGt                                                                                                               <  1:421415/141‑1 (MQ=255)                         tgttatgAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGt                                                                                                               <  2:236926/141‑1 (MQ=255)                              tgAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCAt                                                                                                          >  2:3447/1‑141 (MQ=255)                                 aaTGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGAGTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGGAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAg                                                                                                       <  2:511021/141‑1 (MQ=255)                                     gAAAGAGGCGGAGATCTTCTGTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGCAgggtctggaga                                                                                                   >  2:619698/1‑131 (MQ=255)                                      aaaGAGACGGAGATTTTCTTTGAAAACTTAACTGATTTGTTAGATTTTATGATACACGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGaa                                                                                                  <  2:623959/141‑1 (MQ=255)                                        agagACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACg                                                                                                <  1:216900/141‑1 (MQ=255)                                         gagaCGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGa                                                                                               <  2:643461/141‑1 (MQ=255)                                         gagaCGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGa                                                                                               >  1:13129/1‑141 (MQ=255)                                         gagaCGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAACTAGTAATTGCCATCGGGTGTACGGGTGGACAACATCGATCTGTAGCATTAGTAGAACGa                                                                                               >  1:370915/1‑141 (MQ=255)                                         gagaCGGAGAGTTTCTTTGAAAAATTAACTGATTTGTTAGATGTTATGATACCTGGGTATAAAAAAGAAGGGAAATCTAAATTAGTAATTGCCATCGGTTGTACGGGTGGACCACAACGAACGGCAGCACTAGCAGAACGa                                                                                               >  2:756796/1‑141 (MQ=255)                                          agaCGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGAc                                                                                              <  2:116299/141‑1 (MQ=255)                                           gaCGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACt                                                                                             >  1:177430/1‑141 (MQ=255)                                             cGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCTTTAGCAGAACGACTAg                                                                                           >  2:366112/1‑141 (MQ=255)                                             cGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAg                                                                                           >  2:311743/1‑141 (MQ=255)                                              ggAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAgg                                                                                          >  1:187174/1‑141 (MQ=255)                                                 gaTTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTaa                                                                                       <  1:540011/141‑1 (MQ=255)                                                  aTTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAAt                                                                                      <  1:330486/141‑1 (MQ=255)                                                    tttCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTa                                                                                    <  1:548069/141‑1 (MQ=255)                                                    tttCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTa                                                                                    <  2:608089/141‑1 (MQ=255)                                                    tttCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTa                                                                                    <  1:451192/141‑1 (MQ=255)                                                       cTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCt                                                                                 >  1:572307/1‑141 (MQ=255)                                                           gAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAAt                                                                             <  2:735513/141‑1 (MQ=255)                                                               aaTTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTACGTAATTATCTAAATGAAg                                                                         >  1:482198/1‑141 (MQ=255)                                                                 ttAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTa                                                                       <  1:389453/141‑1 (MQ=255)                                                                    aCTGATTTGTTTTATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTactt                                                                    <  2:335000/141‑4 (MQ=255)                                                                       gATTTGTTAGATTTTATGATCCCCGGGTATAAAAAAGAAGGTAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGaa                                                                 <  1:10577/141‑1 (MQ=255)                                                                          ttGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAatat                                                              <  1:328860/141‑1 (MQ=255)                                                                            gTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACAACTAGGTAATTATCTAAATGAAGTATTTGAATATaa                                                            <  1:184045/141‑1 (MQ=255)                                                                            gTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATaa                                                            <  1:190858/141‑1 (MQ=255)                                                                                    ttATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTAtg                                                    <  1:229620/141‑1 (MQ=255)                                                                                      aTGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTCGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTAtgtg                                                  >  1:158191/1‑141 (MQ=255)                                                                                      aTGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTAtgtg                                                  >  1:780797/1‑141 (MQ=255)                                                                                       tggtACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGc                                                 <  2:521956/138‑1 (MQ=255)                                                                                       tGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGc                                                 <  2:763161/141‑1 (MQ=255)                                                                                        gATACCCGGGTATAAAAAAGAAGGGAAGTCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGca                                                <  1:419683/141‑1 (MQ=255)                                                                                            cccGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGcatcat                                            >  2:170514/1‑141 (MQ=255)                                                                                             ccGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATa                                           >  1:680836/1‑141 (MQ=255)                                                                                                     aaaaaaGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGca                                   <  1:118344/141‑1 (MQ=255)                                                                                                     aaaaaaGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGca                                   >  2:45500/1‑141 (MQ=255)                                                                                                         aagaagGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACata                               <  1:55488/141‑1 (MQ=255)                                                                                                          agaagGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGTCGCACatat                              <  1:690418/141‑1 (MQ=255)                                                                                                          agaagGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACatat                              <  2:179354/141‑1 (MQ=255)                                                                                                          agaagGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACatat                              <  2:39014/141‑1 (MQ=255)                                                                                                           gaagGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATAtt                             <  1:3447/141‑1 (MQ=255)                                                                                                           gaagGGAAATCTCAATTAGTAATTGCCATCGATTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATAtt                             <  2:59853/141‑1 (MQ=255)                                                                                                           gaagGGAAATCTCAATTAGTAAATGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATAtt                             <  2:231208/141‑1 (MQ=255)                                                                                                             agGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGa                           <  1:765977/141‑1 (MQ=255)                                                                                                                gAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGAAAg                        >  2:297302/1‑141 (MQ=255)                                                                                                                  aaTCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGAAAGTg                      >  2:533979/1‑141 (MQ=255)                                                                                                                  aaTCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGAAAGTg                      >  1:360816/1‑141 (MQ=255)                                                                                                                    tctcAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATATGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGATGCACATATTGAAAGTGGc                    >  2:706139/1‑141 (MQ=255)                                                                                                                       caagtggtgaTTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGGGCGACTAGGTAATTATCTAAATGAAGTATTTGAATATGATGTTTATGTGCATCATAGGGACGCACATATTGAAAGTGGCgag                 <  2:482198/132‑1 (MQ=255)                                                                                                                       cAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGAAAGTGGCgag                 <  2:384601/141‑1 (MQ=255)                                                                                                                       cAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCACAGGGACGCACATATTGAAAGTGGCgag                 >  2:119726/1‑141 (MQ=255)                                                                                                                        aaTTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGAAAGTGGCgaga                >  2:422314/1‑141 (MQ=255)                                                                                                                               aaTTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGTATCATAGGGACGCACATATTGAAAGTGGCGAGAAAAAATg         >  2:343848/1‑141 (MQ=255)                                                                                                                                   gCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGAAAGTGGCGAGAAAAAATGAGAc     <  1:113162/141‑1 (MQ=255)                                                                                                                                      aTCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGAAAGTGGCGAGAAAAAATGAGACaaa  >  1:445599/1‑141 (MQ=255)                                                                                                                                          |                                                                                                                                         ATTAGATAAAGACGTTTATAATTATGTTATGAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTGTTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATCGGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTAAATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGAAAGTGGCGAGAAAAAATGAGACAAA  >  CP000730/849519‑849792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑