BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,940	G→A	26.9%	R204R (CGC→CGT)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,940	0	G	A	26.9%	400.9 / 180.0	274	R204R (CGC→CGT)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base G (79/121); new base A (40/34); total (119/155)
Fisher's exact test for biased strand distribution p-value = 3.93e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 282 total aligned reads displayed.

GTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT  >  CP000730/35798‑36080

gtattttaataaAAAT‑GATTGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                                                                                              <  1:610550/135‑1 (MQ=11)

gtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                                                                                              <  2:179410/135‑1 (MQ=11)

 tattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                                             <  2:21088/136‑1 (MQ=11)

  gATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                                                                                              <  1:520639/141‑1 (MQ=255)

   ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                                           >  2:135823/4‑141 (MQ=11)

     cTAATAAAAATAGATGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                                           <  2:243841/141‑1 (MQ=255)

         taaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttt                                                                                                                                     <  2:379984/141‑1 (MQ=11)

                  aaGGTTCTGTTGCAAAGTACAAAAATATAGCTAACAACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGTAGAGACCTGCGGTTCTTTTtatata                                                                                                                              >  2:509128/1‑141 (MQ=2)

                  aaGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtatata                                                                                                                              >  1:724338/1‑141 (MQ=11)

                          gatgaaaaGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCCTAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTaa                                                                                                                      <  2:370189/136‑1 (MQ=0)

                                                                     cAGTGTTCGCTTAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                                           >  2:101033/1‑141 (MQ=11)

                                                                     cAGTGTTCGCTTAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                                           <  2:190187/141‑1 (MQ=11)

                                                                      aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTTCGTGGCATTGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATAc                                                                           <  2:651870/141‑1 (MQ=11)

                                                                      aGTGTTCGTTTAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACt                                                                          >  1:546847/1‑141 (MQ=11)

                                                                       gtgtTCGTTTAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                                                         >  2:742816/1‑141 (MQ=255)

                                                                       gtgtTCGCTTAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                         <  1:523643/141‑1 (MQ=32)

                                                                       gtgtTCGCTTAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                         <  2:445630/141‑1 (MQ=17)

                                                                       gtgtTCGCTTAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                         <  1:324277/141‑1 (MQ=17)

                                                                        tgtTCGTTTAACTTGCTAGCATGATGCTAA‑TTTCGTGGCTTGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt                                                                        >  1:21437/1‑141 (MQ=11)

                                                                          ttCGTTTAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGa                                                                      <  2:41260/141‑1 (MQ=255)

                                                                          ttCGTTTAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGa                                                                      >  2:709382/1‑141 (MQ=11)

                                                                              tttAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                  <  1:515993/140‑1 (MQ=17)

                                                                              tttAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                  <  1:25098/140‑1 (MQ=17)

                                                                              cTTAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                  >  1:228521/1‑141 (MQ=32)

                                                                               ttAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatct                                                                 <  2:47022/141‑4 (MQ=255)

                                                                                tAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                                                <  2:403669/141‑1 (MQ=32)

                                                                                 aaCTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                                               <  2:231398/141‑1 (MQ=17)

                                                                                   cTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                             >  2:147880/1‑141 (MQ=17)

                                                                                   cTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                             <  1:708244/141‑1 (MQ=17)

                                                                                   cTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                             <  1:536188/141‑1 (MQ=17)

                                                                                    ttGCTAGCATGATGCTAA‑TTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtct                                                            <  2:536209/141‑1 (MQ=11)

                                                                                      gCTAGCATGATGCTAA‑TTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtcttt                                                          >  2:314335/1‑141 (MQ=11)

                                                                                      gCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                          >  2:526278/1‑141 (MQ=32)

                                                                                      gCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                          >  1:362104/1‑141 (MQ=17)

                                                                                        tAGCATGATGCTAA‑TTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttct                                                        <  2:671948/141‑1 (MQ=11)

                                                                                        tAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                        <  1:378457/141‑1 (MQ=32)

                                                                                          gCATGATGCTAA‑TTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTa                                                      >  1:190597/1‑141 (MQ=11)

                                                                                           cATGATGCTAA‑TTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTAc                                                     >  1:414569/1‑141 (MQ=255)

                                                                                           cATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTAc                                                     <  2:426343/141‑1 (MQ=17)

                                                                                            atgatgCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACt                                                    >  2:706904/1‑141 (MQ=21)

                                                                                            atgatgCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACt                                                    <  1:38558/141‑1 (MQ=21)

                                                                                             tgatgCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACtt                                                   >  2:278336/1‑141 (MQ=35)

                                                                                             tgatgCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACtt                                                   >  1:490926/1‑141 (MQ=25)

                                                                                              gatgCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                  >  1:164906/1‑141 (MQ=25)

                                                                                               atgCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTa                                                 >  2:203576/1‑141 (MQ=25)

                                                                                                 gCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAat                                               >  2:240509/1‑141 (MQ=25)

                                                                                                 gCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAat                                               >  1:289862/1‑141 (MQ=25)

                                                                                                 gCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACATGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACATTGTCTTTCTTACTTTAat                                               >  2:662059/1‑141 (MQ=255)

                                                                                                    aa‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAAtgtg                                            <  2:580653/141‑1 (MQ=25)

                                                                                                       tttCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTTTTTTTTACTTTAATATGAc                                          >  2:271517/1‑141 (MQ=11)

                                                                                                       tttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGAc                                          <  1:720499/141‑1 (MQ=25)

                                                                                                          cGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGt                                       <  1:671488/141‑1 (MQ=255)

                                                                                                          cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt                                       >  2:479274/1‑141 (MQ=255)

                                                                                                          cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt                                       <  2:329125/141‑1 (MQ=255)

                                                                                                           gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                      >  1:769967/1‑141 (MQ=39)

                                                                                                           gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                      <  1:639142/141‑1 (MQ=255)

                                                                                                           gTGGCATGGCGAAAATCCGGAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                      <  1:85800/141‑1 (MQ=255)

                                                                                                             ggCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGAt                                    >  1:639392/1‑141 (MQ=255)

                                                                                                             ggCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGAt                                    >  1:440641/1‑141 (MQ=255)

                                                                                                             ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt                                    >  2:500681/1‑141 (MQ=255)

                                                                                                              gCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATc                                   <  1:593134/141‑1 (MQ=14)

                                                                                                               cATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCt                                  <  1:463295/141‑1 (MQ=255)

                                                                                                                aTGGGGAAAATCCGTAGATCTGAAGAGACCTACGGCTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                 <  1:614004/141‑1 (MQ=14)

                                                                                                                aTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                 <  2:290775/141‑1 (MQ=39)

                                                                                                                aTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                 >  1:262851/1‑141 (MQ=38)

                                                                                                                aTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                 <  1:756304/141‑1 (MQ=255)

                                                                                                                 tGGGGAAAATCCGTAGATCTGAAGAGACCTACGTTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                                >  1:409838/1‑141 (MQ=11)

                                                                                                                 tGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                                <  1:656459/141‑1 (MQ=39)

                                                                                                                 tGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                                >  1:383352/1‑141 (MQ=255)

                                                                                                                  ggggAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAAGACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                               <  2:303605/138‑1 (MQ=11)

                                                                                                                  ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                               <  2:615141/141‑1 (MQ=255)

                                                                                                                  ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                               <  2:463045/141‑1 (MQ=255)

                                                                                                                    cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                  <  1:185751/104‑1 (MQ=18)

                                                                                                                     gAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAGCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCa                            >  1:580176/1‑141 (MQ=255)

                                                                                                                       aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                          <  2:434040/141‑1 (MQ=255)

                                                                                                                       aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                          <  1:74554/141‑1 (MQ=38)

                                                                                                                       aaaTCCGTAGATCTAAAGAGACCTGCGTTTCTTTTTATATATAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                          <  2:764283/141‑1 (MQ=255)

                                                                                                                        aaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                         <  1:694451/141‑1 (MQ=255)

                                                                                                                        aaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAGTACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                         <  2:80913/141‑1 (MQ=255)

                                                                                                                         aTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATCTCTTGTCTTTCTTACTTTAATATGAGGGTGATCTTGCTCAATGa                        >  1:117592/1‑141 (MQ=11)

                                                                                                                           ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                      <  1:477066/141‑1 (MQ=255)

                                                                                                                           ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                      <  1:422460/141‑1 (MQ=37)

                                                                                                                           ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                      <  2:567047/141‑1 (MQ=255)

                                                                                                                            cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                     >  2:348992/1‑141 (MQ=39)

                                                                                                                            cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                     <  1:63523/141‑1 (MQ=255)

                                                                                                                            cGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTGCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                     >  1:601959/1‑141 (MQ=14)

                                                                                                                              tAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTa                   >  2:399596/1‑141 (MQ=38)

                                                                                                                               aGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAt                  >  2:690820/1‑141 (MQ=255)

                                                                                                                                gATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                 <  2:543934/141‑1 (MQ=255)

                                                                                                                                gATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                 >  1:734013/1‑141 (MQ=255)

                                                                                                                                 aTCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTc                <  1:288211/141‑1 (MQ=255)

                                                                                                                                   cTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg              <  1:124259/141‑1 (MQ=255)

                                                                                                                                   cTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg              <  1:100133/141‑1 (MQ=255)

                                                                                                                                    tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa             <  2:390537/141‑1 (MQ=39)

                                                                                                                                      aaGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGata           >  1:108554/1‑141 (MQ=38)

                                                                                                                                        gagaGCTGCGGTTCGTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATGGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt         <  1:80696/141‑1 (MQ=255)

                                                                                                                                          gaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTc       <  1:484179/141‑1 (MQ=38)

                                                                                                                                           cccTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCg      <  2:151446/140‑1 (MQ=38)

                                                                                                                                           aCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCg      >  1:528727/1‑141 (MQ=38)

                                                                                                                                            ccTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa     >  1:765551/1‑141 (MQ=255)

GTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAA‑TTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT  >  CP000730/35798‑36080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`