Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | CP000730 | 110,904 | G→T | 7.7% | D255Y (GAT→TAT) | USA300HOU_0109 → | staphylococcal tandem lipoprotein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | CP000730 | 110,904 | 0 | G | T | 7.7% | 209.6 / 4.0 | 65 | D255Y (GAT→TAT) | USA300HOU_0109 | staphylococcal tandem lipoprotein |
Reads supporting (aligned to +/- strand): ref base G (31/28); new base T (2/3); total (33/32) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.67e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 |
CTCCAAAGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATG > CP000730/110767‑111044 | cTCCAAAGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATg < 1:309128/141‑1 (MQ=255) cTCCAAAGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATg < 1:17592/141‑1 (MQ=255) aaaGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGtata > 2:37167/1‑141 (MQ=255) aaaGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTAAAAAAAAAAAAAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGtata < 2:105609/141‑1 (MQ=25) ttATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCa < 1:205749/141‑1 (MQ=255) aTTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATg < 1:28263/141‑1 (MQ=255) aTTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATg > 2:309824/1‑141 (MQ=255) aaaaGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCaa < 2:231621/141‑1 (MQ=255) aaaGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAAt > 1:333306/1‑141 (MQ=255) aaaGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAAt > 1:333374/1‑141 (MQ=255) ggTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAg < 1:126179/141‑1 (MQ=255) ggTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTACAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAg < 1:203093/141‑1 (MQ=255) ggTTCAGGGAATTTAAAAGGCTAATCAGTTGGATATAAAGATATTGAATTCAAGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAAGCAg < 2:285285/141‑1 (MQ=255) ttCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGaa > 1:241916/1‑141 (MQ=255) aGGGAATTTAAAAGGCTCATCAGCTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAatat > 2:133561/1‑141 (MQ=255) gggAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATg > 1:311751/1‑141 (MQ=255) ggAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGa > 1:202592/1‑141 (MQ=255) aaaGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAaatcaatc > 1:187785/1‑141 (MQ=255) aaaGGCTAATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAAAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAaatcaatc < 2:251920/141‑1 (MQ=255) aaGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATcc > 2:218111/1‑141 (MQ=255) aGGCTCATCAGTTGGATATAAAGATATTGAAGTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATccc < 1:122750/141‑1 (MQ=255) catcaGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGaaa < 1:212336/141‑1 (MQ=255) atcaGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGaaaa > 2:56828/1‑141 (MQ=255) atcaGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGaaaa > 2:226280/1‑141 (MQ=255) tcaGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGaaaaa < 1:298145/141‑1 (MQ=255) tcaGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGaaaaa > 1:41218/1‑141 (MQ=255) ttGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAagaga < 1:133083/141‑1 (MQ=255) atatAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGtga > 1:241668/1‑141 (MQ=255) tataAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGtgat > 1:231437/1‑141 (MQ=255) tataAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGtgat < 1:314007/141‑1 (MQ=255) aagaaaTATATTGAATTGAAGATTGTAGAGAAAAAAGAAGAAAATATATAATTTAGTGATAAATAAGAAAATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGtgat < 2:303246/138‑1 (MQ=255) aaaGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGaa > 1:259704/1‑141 (MQ=255) aaGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAGAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGaaa < 1:305968/141‑1 (MQ=255) aaGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGaaa > 2:158734/1‑141 (MQ=255) gATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATg > 2:240523/1‑141 (MQ=255) atatTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGa > 1:109324/1‑141 (MQ=255) tatTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGAt < 1:78333/141‑1 (MQ=255) gAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATaaaa > 1:181305/1‑141 (MQ=255) tACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATACAAGAAAAAGAGAGTGATGAAATGATAAAACgtgt < 2:202592/141‑1 (MQ=255) cGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGtaa > 1:226030/1‑141 (MQ=255) cGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGtaa > 1:66281/1‑141 (MQ=255) ttagtaaaaaaaaaaaaAGAAAATATATACTTAAGTGATAGCTTAGATAATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGtaaat < 1:7050/132‑1 (MQ=255) gagagAAAAAAGAAGAAAAGATATACTTTAGGGATAGCTTAGATTAGAAAAAAAGCGGAGATGTATGATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGAGGAAATGATAAAACGTGTaaataaat < 2:218052/140‑1 (MQ=255) agaAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAAATTATAAAAAAAGAAAAACAGTCCAACCATAGATCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAg < 1:197680/141‑1 (MQ=255) gaAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGt > 1:32702/1‑141 (MQ=255) aagaaaGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGTGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTg > 2:282212/4‑141 (MQ=255) aaaaaGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCAAAGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGc < 2:119642/141‑1 (MQ=255) aaaGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCtt < 2:13618/141‑1 (MQ=255) aagaaAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTa > 2:191895/1‑141 (MQ=255) aaaTATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAg > 1:15100/1‑141 (MQ=255) aaaTATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAg > 1:15087/1‑141 (MQ=255) atatatACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGtc < 1:37167/141‑1 (MQ=255) atataCTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGtctt < 1:249530/141‑1 (MQ=255) atataCTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGtctt < 1:256815/141‑1 (MQ=255) ataCTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGtcttct < 2:162688/141‑1 (MQ=255) atcatGATAGCTCAAGTTATAAAAAAAGCGCCGATGTATAATCATAGCTCACTCTTTATATGAAAAAAAACAAAGAAAAAGAGAGTGATGAAATGATAAATCTTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGtttt < 2:12357/137‑1 (MQ=255) aTAGCTTATAATAAAAAAAAAGCGGGGTTGTATAATCATGGCTCAATCAGAATATGAAATCCATCCCGGAAAAAGTGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCa < 2:331026/141‑1 (MQ=255) aGATAATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAACAAATTAGTGCTTGGTATTAGTCTTCTGTTGTTAGTCATTAGTAt > 2:240659/1‑141 (MQ=255) tataAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCAct > 1:145819/1‑141 (MQ=255) ataAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCActg > 1:56101/1‑141 (MQ=255) aaaaaaaGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCActgct > 2:278138/1‑141 (MQ=255) aaaGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGtt < 1:5403/141‑1 (MQ=255) aGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTtgt > 2:226888/1‑141 (MQ=255) aggAGATGTATAATCATGGCTCAATCAGAATATAAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTgg < 1:222261/140‑1 (MQ=255) ggAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTAATAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGc > 1:232665/1‑141 (MQ=255) gagaTGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCa > 2:271732/1‑141 (MQ=255) gaTGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATg > 2:96276/1‑141 (MQ=255) | CTCCAAAGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATG > CP000730/110767‑111044 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |