| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,608,818 | G→A | 14.3% | T20M (ACG→ATG) | USA300HOU_1492 ← | possible lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,608,818 | 0 | G | A | 14.3% | 57.0 / 2.1 | 26 | T20M (ACG→ATG) | USA300HOU_1492 | possible lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (16/6); new base A (2/2); total (18/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
GATGCTTCTTCGCAAATTCAATCGCATTTTTTTCTGCAGGCGGTTGAAAGCCTTGGCCTACATATTTTGAAGCTTCCATTTCTTCTGGTACAGATTTTGTTTCTGTTTTTGTGTCTTTTTTTGATTCATTTTCCATCGTGGAACATCCCCCTAAAATTAATGTCGTAGCTAAAACTGATCCAATGAATTTTTTCATATGACACCTCGAATAATTTAATTAATACAATATATCTATATTTTACCCTATTTTTATAATCAAATAAATAACTTTATCAA > CP000730/1608681‑1608956 | gATGCTTCTTCGCAAATTCAATCGCATTTTTTTCTGCAGGCGGTTGAAAGCCTCGGCCTACATATTTTGAAGCTTCCATTTCTTCTGGTACAGATTTTGTTTCTGTTTTTGTGTCTTTTTTTGATTCATTTTCCATCGTgg > 1:168304/1‑141 (MQ=255) aaTTCAATCGCATTTTTTTCTGCAGGCGGTTGAAAGCCTTGGCCTACATATTTTGAAGCTTCCATTTCTTCTGGTACAGATTTTGTTTCTGTTTTTGTGTCTTTTTTTGATTCATTTTCCATCGTGGAACATCCCCCTaaa < 2:311715/141‑1 (MQ=255) ttCAATCGCATTTTTTTCTGCAGGCGGTTGAAAGCCTTGGCCTACATATTTTGAAGCTTCCATTTCTTCTGGTACAGATTTTGTTTCTGTTTTTGTGTCTTTTTTTGATTCATTTTCCATCGTGGAACATCCCCCTAAAAt < 1:319115/141‑1 (MQ=255) aTCGCATTTTTTTCTGCAGGCGGTTGAAAGCCTTGGCCTACATATTTTGAAGCTTCCATTTCTTCTGGTACAGATTTTGTTTCTGTTTTTGTGTCTTTTTTTTATTTATTTTCCATCGTGTAACATCCCCCTAACATTAAt > 2:316898/1‑141 (MQ=255) cATTTTTTTCTGCAGGCGGTTGAAAGCCTTGGCCTACATATTTTGTAGCTTCCATTTCTTCTGGTACAGATTTTTTTTCTGTTTTTGTGTCTTTTTTTGATTCATTTTCCATCATGGAACATCCCCCTATAATTAATGTCg < 2:221730/141‑1 (MQ=255) cTGCAGGCGGTTGAAAGCCTTGGCCTACATATTTTGAAGCTTCCATTTCTTCTGGTACAGATTTTGTTTCTGTTTTTGTGTCTTTTTTTGATTCATTTTCCATCGTGGAACATCCCCCTAAAATTAATGTCGTAGCTaaaa < 1:59298/141‑1 (MQ=255) gCAGGCGGTTGAAAGCCTTGGCCTACATATTTTGAAGCTTCCATTTCTTCTGGTACAGATTTTGTTTCTGTTTTTGTGTCTTTTTTTGATTCATTTTCCATCGTGGAACATCCCCCTAAAATTAATTTCGTAGCTAAAACt > 1:184447/1‑141 (MQ=255) tGAAAGCCTTTGCCTACATATTTTGAAGCTGCCATTTCTTCTGGTACAGATTTTGTTTCTGTTTTTGTGTCTTTTTTTGATTCATTTTCCATCGTAGAACATCCCCCTATAATTAATGTCGTAGCTAACACTGTTCCAATg < 2:272812/141‑1 (MQ=37) tGGCCTACATATTTTGAAGCTTCCATTTCTTCTGGTACAGATTTTGTTTCTGTTTTTGTGTCTTTTTTTGATTCATTTTCCATCGTGGAACATCCCCCTAAAATTAATGTCGTAGCTAAAACTGATCCAATGAATTTTTTc > 1:186567/1‑141 (MQ=255) ggCCTACATATTTTGAAGCTTCCATTTCTTCTGGTACAGATTTTGTTTCTGTTTTTGTGTCTTTTTTTGATTCATTTTCCATCGTGGAACATCCCCCTAAAATTAATGTCGTAGCTAAAACTGATCCAATGAATTTTTTCa < 2:179121/141‑1 (MQ=255) atTTTGTAGCTTCCATTTCTTCTGGTACAGATTTTGTTTCTGTTTTTGTGTCTTTTTTTGATTCATTTTCCATCGTGGAACATCCCCCTAAAATTAATGTCGTAGCTAAAACTGATCCAATGAATTTTTTCATATGACAcc < 1:16473/141‑1 (MQ=255) aGCTTCCATTTCTTCTGGTACAGATTTTGTTTCTGTTTTTGTGTCTTTTTTTGATTCATTTTCCATCGTGGAACATCCCCCTAAAATTAATGTCGTAGCTAAAACTGATCCAATGAATTTTTTCATATGACACCTCGaata > 1:110064/1‑141 (MQ=255) cTTCCATTTTTTCTGGTACTGATTTTGTTTCTGTATTCGTGTCTTTACTTGATTCATTTTCCATCATGGAACATCCCCCTAAAATTAATGTCGTAGCTAAAACTGATCCAATGAATTTTTTCATATGACACCTCGaataat > 2:244396/1‑141 (MQ=38) tcttctGGTACAGATTTTGTTTCTGTTTTTGTGTCTTTTTTTGATTCATTTTCCATCGTGGAACATCCCCCTAAAATTAATGTCGTAGCTAAAACTGATCCAATGAATTTTTTCATATGACACCTCGAATAATttaattaa > 1:89859/1‑141 (MQ=255) ctGGTACAGATTTTGTGCCTGATTTTGTGTCTTTTTTTGATTCATTTTCCATCGTGGAACCTCGCCCTAAAATTAATGTCGTTGCTAAAACTGACCCACTGAATTTTTTCATATGACACCTCGAATACTTAAACTAATACa > 2:275341/1‑141 (MQ=255) ggTACAGATTTTGTTTCTGTTTTTGTGTCTTTTNTTGATTCATTTTCCATCGTGGAACATCCCCCTAAAATTAATGTCGTAGCTAAAACTGATCCAATGAATTTTTTCATATGACACCTCGAATAATTTAATTAANACAat > 1:42678/1‑141 (MQ=255) gTTTCTGTTTTTGTGTCTTTTTTTGATTCATTTTCCATCGTGGAACATCCCCCTAAAATTAATGTCGTAGCTAAAACTGATCCAATGAATTTTTTTATTTTTTTTCTCGAATAATTTAATTAATACAATATATCTATtttt > 2:298588/1‑141 (MQ=255) cTGTTTTTGTGTCTTTTTTTGATTCATTTTCCATCGTGGAACATCCCCCTAAAATTAATGTCGTAGCTAAAACTGATCCAATGAATTTTTTCATATGACATCTCGAATAATTTAATTAATACAATATATCTATATTTTAcc > 2:225582/1‑141 (MQ=255) tgtgTCTTTTTTTTTTTTATTTTCAATCGTGGAACATCCCCCTAAAATTAATGTCGTAGCTAAAACTGATCCAATGAATTTTTTCATATGACACTTCGAATAATTTAATTAATACAATATATCTATATTTTACCCTAtttt > 2:114157/1‑141 (MQ=255) gtctttacttGATTCATTTTCCATCATGGAACATCCCCCTAAAATTAATGTCGTAGCTAAAACTGATCCAATGAATTTTTTCATATGACACCTCGa > 2:321830/9‑96 (MQ=255) gtCTTTTTTTGATTCATTTTCCATCGTGGAACATCCCCCTAAAATTAATGTCGTAGCTAAAACTGATCCAATGAATTTTTTCATATGACACCTCGAATAATTTAATTAATACAATATATCTATATTTTACCCTATTTTtat > 1:32287/1‑141 (MQ=255) gtCTTTACTTGATTCATTTTCCATCATGGAACATCCCCCTAAAATTAATGTCGTAGCTAAAACTGATCCAATGAATTTTTTCATATGACACCTCGa < 1:321830/96‑1 (MQ=255) aTTCATTTTCCATCGTGGAACATCCCCCTAAAATTAATGTCGTAGCTAAAACTGATCCAATGAATTTTTTCATATGACACCTCGAATAATTTAATTAATACAATATATCTATATTTTACCCTATTTTTATAATCaaataaa > 1:270921/1‑141 (MQ=255) ttttttttCCATCGTGGAACATCCCCCTAAAATTAATGTTGTAGCTAAAACTGATCCAATGAATTTTTTCATATTTTTTTTTTTTTTTTTTAATTAATACAATATATCTATATTTTACCTTATTTTTATAATCaaataact > 2:310763/5‑139 (MQ=255) ttCATTTTCCATCGTGGAACATCCCCCTAAAATTAATGTCGTAGCTAAAACTGATCCAATGAATTTTTTCATATGACACCTCGAATAATTTAATTAATACAATATATCTATATTTTACCCTATTTTTATAATCaaataaat > 2:310729/1‑141 (MQ=255) tCGTGGAACATCCCCCTAAAATTAATGTCGTAGCTAAAACTGATCCAATGAATTTTTTCATATGACACCTCGAATAATTTAATTAATACAATATATCTATATTTTACCCTATTTTTATAATCAAATAAATAACTTTATCaa > 1:282265/1‑141 (MQ=255) | GATGCTTCTTCGCAAATTCAATCGCATTTTTTTCTGCAGGCGGTTGAAAGCCTTGGCCTACATATTTTGAAGCTTCCATTTCTTCTGGTACAGATTTTGTTTCTGTTTTTGTGTCTTTTTTTGATTCATTTTCCATCGTGGAACATCCCCCTAAAATTAATGTCGTAGCTAAAACTGATCCAATGAATTTTTTCATATGACACCTCGAATAATTTAATTAATACAATATATCTATATTTTACCCTATTTTTATAATCAAATAAATAACTTTATCAA > CP000730/1608681‑1608956 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |