| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,372,419 | C→T | 15.1% | intergenic (‑5536/+22) | rpmC ← / ← pbuG | ribosomal protein L29/NCS2 family nucleobase:cation symporter‑2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,372,419 | 0 | C | T | 15.1% | 79.9 / 6.3 | 33 | intergenic (‑5536/+22) | rpmC/pbuG | ribosomal protein L29/NCS2 family nucleobase:cation symporter‑2 |
| Reads supporting (aligned to +/- strand): ref base C (13/15); new base T (3/2); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
GAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATC > CP000730/2372284‑2372556 | gaagaaACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTGCCAATGATTGGTAGTCg < 2:68164/141‑1 (MQ=255) agaaACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTGCCAATGATTGGTAGTCG‑tt < 1:244936/141‑1 (MQ=255) ccAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCt > 1:159563/1‑141 (MQ=255) acaaTTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGa < 2:229283/141‑1 (MQ=255) aagTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAAc < 2:21214/141‑1 (MQ=255) aagTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAAc < 2:226641/141‑1 (MQ=255) atCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTa < 1:232008/141‑1 (MQ=255) ggATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAg < 1:315818/141‑1 (MQ=255) gATATTTAAAGACATTTATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAga < 2:111504/141‑1 (MQ=255) ttAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAgaatgaa > 2:257697/1‑141 (MQ=255) aaGACATTCATAATATCGCTTTATGTTATTTCGCTTATTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAg < 2:99497/141‑1 (MQ=255) cATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTAcc > 1:253554/1‑141 (MQ=255) cATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTa > 2:109863/1‑141 (MQ=255) cGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatacatac < 1:92959/141‑1 (MQ=255) tttATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACAtt > 1:175578/1‑141 (MQ=255) gCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTActt < 2:258096/141‑1 (MQ=255) ttcttATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTActtct > 1:62374/1‑141 (MQ=255) tcttATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTActtctt < 1:150748/141‑1 (MQ=255) cttATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCttt < 2:96427/141‑1 (MQ=255) ttATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGttt > 2:244041/1‑141 (MQ=255) aTCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTGACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGttttt < 2:272994/141‑1 (MQ=255) cTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGAt < 1:60798/141‑1 (MQ=255) aaaGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGc < 2:268600/141‑1 (MQ=255) aaGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCa > 2:190940/1‑141 (MQ=255) aaaaaCGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTa > 2:112097/1‑141 (MQ=255) aaaaaCGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTa > 1:86596/1‑141 (MQ=255) aaaaaCGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTa < 2:124776/141‑1 (MQ=255) cGACTGCCAATGATTGGTAGTCGTTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTg > 2:279653/1‑141 (MQ=255) aCCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGa > 2:84540/1‑141 (MQ=255) cAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGaaa > 1:73578/1‑141 (MQ=255) aatgaTTGGTAGTCGTTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGaaa > 1:16987/5‑141 (MQ=255) gaTTGGTAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAaaataa > 1:288373/2‑141 (MQ=255) tGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATTAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATc > 2:193107/1‑141 (MQ=255) | GAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATC > CP000730/2372284‑2372556 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |