Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,670,935 G→A 14.8% A60V (GCG→GTG)  USA300HOU_2525 ← esterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,670,9350GA14.8% 58.8 / 3.5 27A60V (GCG→GTG) USA300HOU_2525esterase
Reads supporting (aligned to +/- strand):  ref base G (11/12);  new base A (2/2);  total (13/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

CATGTGCATATAATACAACGCGTTGATGCTTATCTTGTTTATCGTTAACGGTATAAACGGTTGAACCGAAGTGCTTATCCACTTTTACTGGCATCTTAAACTGATAATTGGTTCCCTCATATTCGCCTGCTGTCTGTGGCGCCATTTTTTCTAATGCTTTCATCGCTTCTTCTTTATACATAAAACGACGCTTAGCTCTAGATAATCGTATACCTTGTTCTAACATATAACTTTTCACACTGCGTTTTTCTTTAATTCTAATATGTGCGTATGCTGCAAC  >  CP000730/2670795‑2671074
                                                                                                                                            |                                                                                                                                           
cATGTGCATATAATACAACGCGTTGATGCTTATCTTGTTTATCGTTAACGGTATAAACGGTTGAACCGAAGTGCTTATCCACTTTTACTGGCAGCTTAAACTGATAATTGGTTCCCTCATATTCGCCTGCTGTCTGTGgcg                                                                                                                                             <  2:301409/141‑1 (MQ=255)
 aTGTGCATATAATACAACGCGTTGATGCTTATCTTGTTTATCGTTAACGGTATAAAAGGTTGAACCGAAGTGCTTATCCACTTTTACTGGCATCTTAAACTGATAATTGGTTCCCTCATATTCGCCTGCTGTCTGTGgcgc                                                                                                                                            >  1:256998/1‑141 (MQ=255)
              aCAACGCGTTGATGCTTATCTTGTTTATCGTTAACGGTATAAACGGTTGAACCGAAGTGCTTATCCACTTTTACTGGCATCTTAAACTGATAATTGGTTCCCTCATATTCGCCTGCTGTCTGTGGCGCCATTTTTTCTAAt                                                                                                                               <  1:141293/141‑1 (MQ=255)
                  cgcgTTGATGCTTATCTTGTTTATCGTTAACGGTATAAACGGTTGAACCGAAGTGCTTATCCACTTTTACTGGCATCTTAAACTGATAATTGGTTCTCTCATATTCGCCTGCTGTCTGTGGCGCCATTTTTTCTAATGCtt                                                                                                                           >  2:292203/1‑141 (MQ=255)
                          tGCTTATCTTGTTTATCGTTAACGGTATAAACGGTTGAACCGAAGTGCTTATCCACTTTTACTGGCATCTTAAACTGATAATTGGTTCCCTCATATTCGCCTGCTGTCTGTGGCGCCATTTTTTCTAATGCTTTCATCGct                                                                                                                   <  2:119324/141‑1 (MQ=255)
                              caTCTTGTTTATCGGTAACGGTATAAACGGTTGAACCTAAGTGCTTATCCACTTTTACTGTCATCTTAAACTGATAATTGCATACCTCATATTCCCCTGCTGTCTGCGGCGCCATTTTTTCTAATGCTTTCATCGcttctt                                                                                                               >  2:101549/2‑141 (MQ=255)
                                   tGTTTATCGTTAACGGTATAAACGGTTGAACCGCAGTGCTTATCCACTTTTACTGGCATCTTAAACTGATAATTGGTTCCCTCATATTCGCCTGCTGTCTGTGGCGCCATTTTTTCTAATGCTTTCATCGCTTCTTCTTta                                                                                                          >  2:272369/1‑141 (MQ=255)
                                   tGTTTATCGTTAACGGTATAAACGGTTGAACCGAAGTGCTTATCCACTTTTACTGGCATCTTAAACTGATAATTGGTTCCCTCATATTCGCCTGCTGTCTGTGGCGCCATTTTTTCTAATGCTTTCATCGCTTCTTCTTta                                                                                                          <  1:5768/141‑1 (MQ=255)
                                      ttATCGTTAACGGTATAAACGGTTGAACCGAAGTGCTTATCCACTTATACTGGCACCTCAAACTGATAATTGGTTCACTCATATTCGCCTGCTGTCTGTGGCGCCATTTTTTCTAATGCTTTCATAGATTCTTCATTATAc                                                                                                       >  2:196722/1‑141 (MQ=255)
                                      ttATCATTAACGGTATAAACGGTTGAACCGAAGTGCTTATCCACTTTTACTGGCATCTTAAACTGATAATTGGTTCCCTCATATTCGCCTGCTGTCTGTGGCGCCATCTTTTCTAATGCTTTCATCGCTTCTTCTTTATAc                                                                                                       >  2:252980/1‑141 (MQ=255)
                                         tCGTTAACGGTATAAACGGTTGAACCGAAGTGCTTATCCACTTTTACTGGCATCTTAAACTGATAATTGGTTCCCTCATATTCGCCTGCTGTCTGTGGCGCCATTTTTTCTAATGCTTTCATCGCTTCTTCTTTATACATa                                                                                                    >  2:220533/1‑141 (MQ=255)
                                           gTTAACGGTATAAACGGTTGAACCGAAGTGCTTATCCACTTTTACTGGCATCTTAAACTGATAATTGGTTCCCTCATATTCGCCTGCTGTCTGTGGCGCCATTTTTTCTAATGCTTTCATCGCTTCTTCTTTATACATaaa                                                                                                  <  2:198930/141‑1 (MQ=255)
                                                cGGTATAAACGGTTGAACCGAAGTGCTTATCCACTTTTACTGGCATCTTAAACTGATAATTGGTTCCCTCATATTCGCCTGCTGTCTGTGGCGCCATTTTTTCTAATGCTTTCATCGCTTCTTCTTTATACATAAAacgac                                                                                             >  1:139507/1‑141 (MQ=255)
                                                  gTATAAACGGTTGAACCGAAGTGCTTATCCACTTTTACTGGCATCTTAAACTGATAATTGGTTCCCTCATATTCGCCTGCTGTCTGTGGCGCCATTTTTTCTAATGCTTTCATCGCTTCTTCTTTATACATAAAACGACGc                                                                                           <  1:307087/141‑1 (MQ=255)
                                                       aaCGGTTGAACCGAAGTGCTTATCCACTTTTACTGGCATCTTAAACTGATAATTGGTTCCCTCATATTCGCCTGCTGTCTGTGGCGCCATTTTTTCTAATGCTTTCATCGCTTCTTCTTTATACATAAAACGACGCTTAGc                                                                                      >  1:221570/1‑141 (MQ=255)
                                                                      gTGCTTATCCACTTTTACTGGCATCTTAAACTGATAATTGGTTCCCTCATATACGCCTGCTGTCTGTGGCGCCATTGTTCCTAAGGCTTTCCTCGCATCTTCTTTATACATAAAACGCCGCTTAGCTCTAGATAATCGcat                                                                       <  2:148569/141‑1 (MQ=255)
                                                                        gCTTATCCACTTTTACTGGCATCTTAAACTGATAATTGGTTCCCTCATATTCGCCTGCTGTCTGTGGCACCATTTTTTCTAATGCTTTCATCGCTTCTTCTTTATACATAAAACGACGCTTAGCTCTAGATAATCGTATAc                                                                     >  1:211088/1‑141 (MQ=255)
                                                                        gCTTATCCACTTTTACTGGCATCTTAAACTGATAATTGGTTCCCTCATATTCGCCTGCTGTCTGTGGCACCATTTTTTCTAATGCTTTCATCGCTTCTTCTTTATACATAAAACGACGCTTAGCTCTAGATAATCGTATAc                                                                     >  1:211082/1‑141 (MQ=255)
                                                                                       cTGGCATCTTAAACTGATAATTGGTTCCCTCATATTCGCCTGCTGTCTGTGGCGCCATTTTTTCTAATGCTTTCATCGCTTCTTCTTTATACATAAAACGACGCTTAGCTCTAGATAATCGTATACCTTGTTCTAACatat                                                      <  1:73269/141‑1 (MQ=255)
                                                                                                   aCTGATAATTGGTTCCCTCATATTCGCCTGCTGTCTGTGGCGCCATTTTTTCTAATGCTTTCATCGCTTCTTCTTTATACATAAAACGACGCTTAGCTCTAGATAATCGTATACCTTGTTCTAACATATAACTTTTCacac                                          >  2:230200/1‑141 (MQ=255)
                                                                                                   aCTGATAATTGGCTCCCTCATATTCGCCTGCTGTCTGTGGCGCCATTTTTTCTAATGCTTTCATCGCTTTTTCTTTATACATAAAACGACGCTTAGCTCTTGATAATCGTATACCTTGTTCTAACATATATCTTTTCacac                                          <  2:268154/141‑1 (MQ=255)
                                                                                                        tAATTGGTTCCCTCATATTCGCCTGCTGTCTGTGGCGCCATTTTTTCTAATGCTTTCATCGCTTCTTCTTTATACATAAAACGACGCTTAGCTCTAGATAATCGTATACCTTGTTCTAACATATAACTTTTCACACTGCGt                                     >  1:188918/1‑141 (MQ=255)
                                                                                                         aaTTGGTTCCCTCATATTCGCCTGCTGTCTGTGGCGCCATTTTTTCTAATGCTTTCATCGCTTCTTCTTTATACATAAAACGACGCTTAGCTCTAGAGAATCGTATACCTTGTTCTAACATATAACTTTTCACACTGCGtt                                    <  2:182432/141‑1 (MQ=255)
                                                                                                            tGGTTCCCTCATATTCGCCTGCTGTCTGTGGCGCCATTTTTTCTAATGCTTTCATCGCTTCTTCTTTATACATAAAACGACGCTTAGCTCTAGATAATCGTATACCTTGTTCTAACATATAACTTTTCACACTGCGttttt                                 <  2:46188/141‑1 (MQ=255)
                                                                                                               ttCCCTCATATTCGCCTGCTGTCTGTGGCGCCATTTTTTCTAATGCTTTCATCGCTTCTTCTTTATACATAAAACGACGCTTAGCTCTAGATAATCGTATACCTTGTTCTAACATATAACTTTTCACACTGCGTTTTTCtt                              <  2:69191/141‑1 (MQ=255)
                                                                                                                      atatTCGCCTGCTGTCTGTGGCGCCATTTTTTCTAATGCTTTCATCGCTTCTTCTTTATACATAAAACGACGCTTAGCTCTAGATAATCGTATACCTTGTTCTAACATATAACTTTTCACACTGCGTTTTTCTTTAATTCt                       <  2:190884/141‑1 (MQ=255)
                                                                                                                                 ctgtctgtGGCACCATTTTTTCTAATGCTTTCATCGCTTCTTCTTTATACATAAAACGACGCTTAGCTCTAGATAATCGTATACCTTGTTCTAACATATAACTTTTCACACTGCGTTTTTCTTTAATTCTAATATGTGCGt            <  2:211082/141‑1 (MQ=255)
                                                                                                                                 ctgtctgtGGCACCATTTTTTCTAATGCTTTCATCGCTTCTTCTTTATACATAAAACGACGCTTAGCTCTAGATAATCGTATACCTTGTTCTAACATATAACTTTTCACACTGCGTTTTTCTTTAATTCTAATATGTGCGt            <  2:211088/141‑1 (MQ=255)
                                                                                                                                  tgtctgtGGCGCCATTTTTTCTAATGCTTTCATCGCTTCTTCTTTACACATAAAACGACGCTTAGCTCTAGATAATCGTATACCTTGTTCTAACATATAACTTTTCACACTGCGTTTTTCTTTAATTCTAATATGTGCGTa           >  2:290078/1‑141 (MQ=255)
                                                                                                                                           cgcCATTTTTTCTAATGCTTTCATCGCTTCTTCTTTATACATAAAACGACGCTTAGCTCTAGATAATCGTATACCTTGTTCTAACATATAACTTTTCACACTGCGTTTTTCTTTAATTCTAATATGTGCGTATGCTGCAAc  >  1:157474/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                           
CATGTGCATATAATACAACGCGTTGATGCTTATCTTGTTTATCGTTAACGGTATAAACGGTTGAACCGAAGTGCTTATCCACTTTTACTGGCATCTTAAACTGATAATTGGTTCCCTCATATTCGCCTGCTGTCTGTGGCGCCATTTTTTCTAATGCTTTCATCGCTTCTTCTTTATACATAAAACGACGCTTAGCTCTAGATAATCGTATACCTTGTTCTAACATATAACTTTTCACACTGCGTTTTTCTTTAATTCTAATATGTGCGTATGCTGCAAC  >  CP000730/2670795‑2671074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑