| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,855,054 | G→T | 11.8% | T190T (ACC→ACA) | rarD ← | DMT superfamily drug/metabolite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,855,054 | 0 | G | T | 11.8% | 107.4 / 5.0 | 34 | T190T (ACC→ACA) | rarD | DMT superfamily drug/metabolite transporter |
| Reads supporting (aligned to +/- strand): ref base G (20/10); new base T (2/2); total (22/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
GAATAGGATTAGTGGTATTGCCGTAATAGCACCAGAAAATAACAACCAAAATGATGACATGTTCAATCCAAATGACATCTGATGTTGCTGCCATAAATAAATAACGTATATTAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCGTATGTACCAAAGGATAACGCTAATATAATAGAGACGATTGG‑GAATTCTCCAATCTTGAGCGTCATATATAATA > CP000730/2854928‑2855193 | gaATAGGATTAGTGGTATTGCCGTAATAGCACCAGAAAATAACAACCAAAATGATGACATGTTCAATCCAAATGACATCTGATGTTGCTGCCATAAATAAATAACGTATATTAGTCCAGCAGGTGCTGTAACAATACATTc > 1:273714/1‑141 (MQ=255)gaATAGGATTAGTGGTATTGCCGTAATAGCACCAGAAAATAACAACCAAAATGATGACATGTTCAATCCAAATGACATCTGATGTTGCTGCCATAAATAAATAACGTATATTAGTCCAGCAGGTGCTGTAACAATACATTc > 1:85663/1‑141 (MQ=255) aaTAGCACCAGAAAATAACAACCAAAATGATGACATGTTCAATCCAAATGACATCTGATGTTGCTGCCATAAATAAATAACGTATATTAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATc < 1:154607/141‑1 (MQ=255) aGAAAATAACAACCAAAATGATGACATGTTCAATCCAAATGACATCTGATGTTGCTGCCATAAATAAATAACGTATATTAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGtac < 1:204884/141‑1 (MQ=255) aaaTAACAACCAAAATGATGACATGTTCAATCCAAATGACATCTGATGTTGCTGCCATAAATAAATAACGTATATTAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGtactac > 1:22184/1‑141 (MQ=255) aaTAACAACCAAAATGATGACATGTTCAATCCAAATGACATCTGATGTTGCTGCCATAAATAAATAACGTATATTAGTCCAGCAGGTGCg > 1:197491/1‑90 (MQ=255) aaTAACAACCAAAATGATGACATGTTCAATCCAAATGACATCTGATGTTGCTGCCATAAATAAATAACGTATATTAGTCCAGCAGGTGCg < 2:197491/90‑1 (MQ=255) acaacCAAAATGATGACATGTTCAATCCAAATGACATCTGATGTTGCTGCCATAAATAAATAACGTATATTAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACttttt < 1:55842/141‑1 (MQ=255) acCAAAATGATGACATGTTCAATCCAAATGACATCTGATGTTGCTGCCATAAATAAATAACGTATATTAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCa < 2:27907/141‑1 (MQ=255) aaaTGATGACATGTTCAATCCAAATGACATCTGATGTTGCTGCCATAAATAAATAACGTATATTAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCaataa > 1:135685/1‑141 (MQ=255) gatgaCATGTTCAATCCAAATGACATCTGATGTTGCTGCCATAAATAAATAACGTATATTAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCg > 2:92836/1‑141 (MQ=255) gaCATGTTCAATCCAAATGACATCTGATGTTGCTGCCATAAATAAATAACGTATATTAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCGTAt > 2:131972/1‑141 (MQ=255) aTCCAAATGACATCTGATGTTGCTGCCATAAATAAATAACGTATATTAGTCCAGCAGGTGCGGTAACAACACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCGTATGTACCAAAgg > 2:99067/1‑141 (MQ=255) aTCCAAATGACATCTGATGTTGCTGCCATAAATAAATAACGTATATTAGTCCAGCAGGTGCGGTAACAACACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCGTATGTACCAAAgg > 2:99066/1‑141 (MQ=255) cATCTGATGTTGCTGCCATAAATAAATAACGTATATTAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTACTTATGGCATCAATATGTTCTACTTTTTTCAATTATCCTTTTATTCCAAAGGATAACGCgaa > 2:310979/1‑141 (MQ=255) cagatgatGCTGCCATAAATAAATAACGTATATTAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATAATGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCGTATGTACCAAAGGATAACGCtaatat > 2:256011/3‑141 (MQ=255) cTGATGTTGCTGCCATAAATAAATAACGTATATTAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCGTATGTACCAAAGGATAACGCtaatat > 2:168801/1‑141 (MQ=255) gATGTTGCTGCCATAAATAAATAACGTATATTAGTCCAGCAGGTGCTGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCATATGTACCAAAGGATAACGCtaatataa < 2:85663/141‑1 (MQ=255) gATGTTGCTGCCATAAATAAATAACGTATATTAGTCCAGCAGGTGCTGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCATATGTACCAAAGGATAACGCtaatataa < 2:273714/141‑1 (MQ=255) gctgcCATAAATAAATAACGTATATTAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCGTATGTACCAAAGGATAACGCTAATATAATAgaga > 2:221777/1‑141 (MQ=255) taaCGTATATTAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCGTATGTACCAAAGGATAACGCTAATATAATAGAGACGATTGG‑GAATtctc < 2:33762/141‑1 (MQ=255) taaCGTATATTAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCGTATGTACCAAAGGATAACGCTAATATAATAGAGACGATTGG‑GAATtctc < 2:89937/141‑1 (MQ=255) taaCGTATATTAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCGTATGTACCAAAGGATAACGCTAATATAATAGAGACGATTGG‑GAATtctc > 2:314806/1‑141 (MQ=255) aaCGTATATTAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCGTATGTACCAAAGGATAACGCTAATATAATAGAGACGATTGGAGAATtccc < 1:250042/141‑3 (MQ=255) tataTTAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATATTCTTTATGTACCAAAGGATAACGCTAATATAATAGAGACGATTGG‑GAATTCTCCAATc > 2:171872/1‑141 (MQ=255) tataTTAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCGTATGTACCAAAGGATAACGCTAATATAATAGAGACGATTGG‑GAATTCTCCAATc < 2:176838/141‑1 (MQ=255) tatTAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCGTATGTACCAAAGGATAACGCTAATATAATAGAGACGATTGG‑GAATTCTCCAATCtt < 2:31268/141‑1 (MQ=255) tatTAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCGTATGTACCAAAGGATAACGCTAATATAATAGAGACGATTGG‑GAATTCTCCAATCtt < 2:77462/141‑1 (MQ=255) atTAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCGTATGTACCAAAGGATAACGCTAATATAATAGAGACGATTGG‑GAATTCTCCAATCTTg > 1:102985/1‑141 (MQ=255) ttAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCGTATGTACCAAAGGATAACGCTAATATAATAGAGACGATTGG‑GAATTCTCCAATCTTGa > 1:265025/1‑141 (MQ=255) ttAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTACTGATGGCATCAATATGTACTACTTTTTTCAATAATCCGTATGTACCAAAGCATAACTCTAATATAATTGAGACGAATAG‑TAATTCTCCAATCTTGa > 2:194694/1‑141 (MQ=255) aGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCGTATGTACCAAAGGATAACGCTAATATAATAGAGACGATTGG‑GAATTCTCCAATCTTGAGc > 2:147492/1‑141 (MQ=255) gcagGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTTTTTTATTTTTATGTACCAAAGGATAACGCTAATATAATAGAGACGATTGG‑GAATTCTCCAATCTTGAGCGTCata > 2:86139/1‑141 (MQ=255) gcagGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCGTATGTACCAAAGGATAACGCTAATATAATAGAGACGATTGG‑GAATTCTCCAATCTTGAGCGTCata > 2:86165/1‑141 (MQ=255) gcagGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCGTATGTACCAAAGGATAACGCTAATATAATAGAGACGATTGG‑GAATTCTCCAATCTTGAGCGTCata > 1:92261/1‑141 (MQ=255) cGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCGTATGTACCAAAGGATAACGCTAATATAATAGAGACGATTGG‑GAATTCTCCAATCTTGAGCGTCATATataata > 1:288762/1‑141 (MQ=255) | GAATAGGATTAGTGGTATTGCCGTAATAGCACCAGAAAATAACAACCAAAATGATGACATGTTCAATCCAAATGACATCTGATGTTGCTGCCATAAATAAATAACGTATATTAGTCCAGCAGGTGCGGTAACAATACATTCAATCGTAATACTGCTGATGGCATCAATATGTACTACTTTTTTCAATAATCCGTATGTACCAAAGGATAACGCTAATATAATAGAGACGATTGG‑GAATTCTCCAATCTTGAGCGTCATATATAATA > CP000730/2854928‑2855193 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |