New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 52489 =85 (0.740)28 (0.270) 6/264 8.0 23.7% intergenic (+99/‑574) USA300HOU_0043/USA300HOU_0044 hypothetical membrane protein/possible transcriptional regulator
?CP000730 = 52983 104 (1.010)intergenic (+593/‑80) USA300HOU_0043/USA300HOU_0044 hypothetical membrane protein/possible transcriptional regulator
Rejected: Coverage evenness skew score above cutoff.

CGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/52650‑52489
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tatttcacgcgatttTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCT  <  CP000730/52983‑52837
                                                                                                                                                                                                                                                                                                                     
CGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCCCGTGATTTTCGCGCGATTTTTACGATGATTTCAAACGCTTCCCCTGATT                                                                                                                                                                   >  1:997704/1‑148
CGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTATTCCGATGATTTCAAACGATTCCTCTGATT                                                                                                                                                                   >  1:1151729/1‑148
 GCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTTCTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCACGCGATTTTTATTATGATTTAAACATATTCCTCTGATTA                                                                                                                                                                  >  2:194036/1‑148
 GCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCCCGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTA                                                                                                                                                                  >  1:231312/1‑148
     TTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGTTTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGTGATTTTTATGATGTTTTTAAATGTTTTCTCTGATTATTTa                                                                                                                                                              >  2:554124/1‑147
      TACTTCGATGATTTTCAACGATTCCTCTGTTTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGAGTTCAAACGATTCCTCTGATTATTTCA                                                                                                                                                             <  1:660810/148‑1
        CTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACG                                                                                                                                                           >  1:801689/1‑148
          ACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCACACGATTCCTCTGATTATTTCACGTGATTTTCGCACCATTACTACGATGGTTGTAAACGTTTTATTTGATTATTTCACctg                                                                                                                                                         >  2:163391/1‑145
          ACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCG                                                                                                                                                         <  2:390370/148‑1
           cgatcgTTTCAAACGATTCCTCTTATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGA                                                                                                                                                        <  1:513825/142‑1
            GATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGGTTCCTCTGATTATTTCACGTGATTTTCGCGCGATTATTCCGATGTTTTCAAACGTTTCTTTTGATTATTTTACGggtt                                                                                                                                                       >  2:994453/1‑144
                ATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCAATTACTACGATGTTTTCAAACGAATACTCTTATTATTTtccctgatttta                                                                                                                                                   >  2:903065/1‑136
                                     TATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCACGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCAAGATTATTGCGATGATTa                                                                                                                              >  2:1099798/1‑147
                                                      GCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGTGATTTTTAGTAGGAGTCCAAACGTTTCCTCTGAT                                                                                                             >  2:62247/1‑148
                                                        gctatttaTACGATGTTTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCGGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTA                                                                                                           <  2:569404/140‑1
                                                        GCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATT                                                                                                                                                                   >  1:1209328/1‑92
                                                        GCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATT                                                                                                                                                                   >  1:528831/1‑92
                                                        GCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATT                                                                                                                                                                   <  2:1209328/92‑1
                                                        GCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATT                                                                                                                                                                   <  2:528831/92‑1
                                                                          TCAAACGATTCCTCTGATTATTTCACGTGCTTTTCGCGCGATTGCTACGATGGTTTCAACCGATGCCTCTGATTATTTCACTCTATTTTCTCTCTATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCG                                                                                         <  2:1235915/148‑1
                                                                                               TTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCATCTGATTTTTTCACGAGATTTTCGCCACATTACTACAATAATTTCAA                                                                    >  1:542130/1‑148
                                                                                                TCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTT                                                                                                                                                               <  1:264384/56‑1
                                                                                                         tctcgcttGATCACTACGATGATTTTAAACGATTTCCCTGATTATTTCACGCGATTTCCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTC                                                          <  2:330993/140‑1
                                                                                                          ttctccCGATCACTACGATGATTTCAAACTATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATGCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCT                                                         <  1:362203/142‑1
                                                                                                          tcCGCGCGACTACTATCATGTTTTCAAACCATTCCTCTGCTTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCT                                                         <  2:994936/146‑1
                                                                                                          TTCGAGCTATTACTATGATGATTTCAAACGATCGCTCGTATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCT                                                         <  2:638989/148‑1
                                                                                                           tctcactATTACTACTATTTTTTCCAACGATCCCTCTTTCTATTTCACGCGATTTTCGCGCGATTACTACGATTATTTCAAACGATCCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTG                                                        <  2:588517/141‑1
                                                                                                           TCGCGCGATTACTACGATGATTTCAAGCGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGCTTTCAAACGATTCCTCTGATTATTTCACGAGATTGTTGTGCTATTACTACGATGATTTCTTACGATTCCTCTG                                                        <  1:917745/148‑1
                                                                                                            CGCGCGATTACTACGATGATTTCAAACGGTTCCTCTGAATATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGA                                                       <  1:105406/148‑1
                                                                                                            CGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGA                                                       <  1:96991/148‑1
                                                                                                            CGCGCGATTACTACGATGATTTCAAACCATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGA                                                       <  1:1089825/148‑1
                                                                                                              CGCGATTACTACGATGATTTCAAACGATCCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATT                                                     <  1:655317/148‑1
                                                                                                                 GATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCC                                                                                                                   >  1:1255957/1‑83
                                                                                                                 GATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCC                                                                                                                   >  1:318592/1‑83
                                                                                                                   TTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGA                                                                                                                                 >  1:1088892/1‑67
                                                                                                                       TACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCT                                                                                                                >  1:862180/1‑80
                                                                                                                        ACGATGATTTCAAACGATTCCTCTGATTATTTCACGC                                                                                                                                                          >  1:602043/1‑37
                                                                                                                         CGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTG                                                                                                               >  1:1224256/1‑79
                                                                                                                           ATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCG                                                                                         >  1:935334/1‑99
                                                                                                                           ATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCG                                                                                         >  1:57686/1‑99
                                                                                                                           ATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCG                                                                                         >  1:308621/1‑99
                                                                                                                               TTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATT                                                                                                                     >  2:585975/1‑67
                                                                                                                                    AACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTC                                                                      >  1:1237246/1‑109
                                                                                                                                    AACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTC                                                                      >  1:489005/1‑109
                                                                                                                                    AACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTC                                                                                                                             >  1:18644/1‑54
                                                                                                                                               TGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTG                                                        >  2:861446/1‑112
                                                                                                                                                   TATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACG                                                                              >  2:159861/1‑86
                                                                                                                                                    ATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCACACGAAAACTCTGATTCTTTCACTCTAGTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTC               >  1:1235915/1‑148
                                                                                                                                                    ATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCG                                                                                         >  1:781804/1‑74
                                                                                                                                                       TCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTT                                                                                                        >  2:264384/1‑56
                                                                                                                                                          CGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGA                                                                             >  1:883517/1‑80
                                                                                                                                                          CGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGA                                                                             >  1:551131/1‑80
                                                                                                                                                          CGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATT                                                                                    >  1:323345/1‑73
                                                                                                                                                          CGCGATTTTCGCGCGATTACTACGATGATTTCAAACGAT                                                                                                                      <  2:262032/39‑1
                                                                                                                                                          CGCGATTTTCGCGCGATTACTACGATGATTTCAAACGAT                                                                                                                      >  1:262032/1‑39
                                                                                                                                                           GCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATT                                                     >  1:924859/1‑103
                                                                                                                                                           GCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATT                                                     >  1:924805/1‑103
                                                                                                                                                           GCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATT                                                     >  1:629230/1‑103
                                                                                                                                                           GCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATT                                                     <  2:924859/103‑1
                                                                                                                                                           GCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGAT                                                                         >  1:245066/1‑83
                                                                                                                                                           GCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATT                                                                                                            >  1:818612/1‑48
                                                                                                                                                           GCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATT                                                                                                            <  2:818612/48‑1
                                                                                                                                                                 TTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCT  <  1:1144113/148‑1
                                                                                                                                                                                                                                                                                                                     
CGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/52650‑52489
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tatttcacgcgatttTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCT  <  CP000730/52983‑52837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.