BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	36,378	C→T	L58L (TTG→TTA)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,378	0	C	T	92.3%	4072.0 / 269.4	1993	L58L (TTG→TTA)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (83/68); new base T (951/868); total (1049/944)
Fisher's exact test for biased strand distribution p-value = 5.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1994 total aligned reads displayed.

CTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTA  >  CP000730/36234‑36525

      cAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGCTATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTtgg                                                                                                                                            >  2:663080/1‑148 (MQ=11)

      cAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTTTTTTTCTTCCCAGTTTGCTCTAAAAtagtg                                                                                                                                            >  2:316562/1‑144 (MQ=11)

      cAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATTTACGTCTCATCAATAAGCCATTTGTAATAAGATTTTTTATGCTTTTTCTTACAAATTTGATATaagtttatg                                                                                                                                            >  1:457461/1‑141 (MQ=11)

      cAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATAAAAAATTtgg                                                                                                                                            >  1:495478/1‑148 (MQ=255)

       aaCCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCAAAATTTGAAATAAAAttagag                                                                                                                                           >  2:733327/1‑144 (MQ=11)

        aCCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTTTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGc                                                                                                                                          >  2:1166237/1‑148 (MQ=255)

          cAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                                                                                        <  1:1046146/148‑1 (MQ=37)

           aaaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCatt                                                                                                                                       >  1:1151144/1‑147 (MQ=255)

           aaaTATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                                                                                                                                       >  2:863342/1‑148 (MQ=37)

             atatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATAtt                                                                                                                                     <  2:1141085/148‑1 (MQ=255)

                 cTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg                                                                                                                                 <  1:1023212/148‑1 (MQ=255)

                  tAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGa                                                                                                                                >  1:1123423/1‑148 (MQ=38)

                  tAATGTATGTCCCTCCGTACCAATGGCCCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGTTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGa                                                                                                                                <  1:121425/148‑1 (MQ=11)

                   aaTGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATTGTCTTGaa                                                                                                                               >  2:957178/1‑148 (MQ=255)

                      gtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAccc                                                                                                                            <  2:1215179/148‑1 (MQ=38)

                        atgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATATGCTTTTTTATGTTTTTTTTTCCTAATTTGGTATAATATTGGGGGTTTTTCTTGatgctac                                                                                                                          >  1:476083/1‑142 (MQ=11)

                        atgtCCCTCTGCATCAATGACACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATATTTGAACCCaa                                                                                                                          >  2:715246/1‑148 (MQ=255)

                            cccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGt                                                                                                                      >  1:582399/1‑148 (MQ=255)

                            cccTCTGCATCAATGGCACGATGTAAATAGCTCCATTTGCCTTTTATTTTGATGTACGTCTCATCAATACTCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTTGGGCATATTCTTGAACCCAACGGt                                                                                                                      >  2:1239836/1‑148 (MQ=11)

                            cccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATACAATTGGGGCATATTCTTGAACCCAACGGt                                                                                                                      >  1:381750/1‑148 (MQ=25)

                             ccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGtg                                                                                                                     >  2:239481/1‑147 (MQ=255)

                             ccTCTGTATCAATGGCACGATATAAATAACTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAACCTACGGtc                                                                                                                     >  1:669203/1‑147 (MQ=11)

                             ccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGCCATATTCTTGAACCCACCGGTa                                                                                                                     >  2:79848/1‑148 (MQ=255)

                                  gtaTCAATGGCACGATATAAATAGCTGCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCg                                                                                                                <  1:756754/146‑1 (MQ=11)

                                  gtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATCCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCg                                                                                                                <  1:359169/146‑1 (MQ=255)

                                  gtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCg                                                                                                                <  1:13309/146‑1 (MQ=255)

                                      cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGa                                                                                                            <  1:210476/148‑1 (MQ=21)

                                      cAATGGCACGATATAAATAGCAACATAACTCTTGTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATGCTTGAACCCAACGGTAGACCGCTGa                                                                                                            >  2:886877/1‑148 (MQ=11)

                                       aaTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGaa                                                                                                           >  1:522368/1‑148 (MQ=25)

                                         tGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatg                                                                                                         <  1:418069/148‑1 (MQ=25)

                                          ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCAACAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatac                                                                                                        >  2:1057164/1‑146 (MQ=255)

                                          ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                                                                                        <  1:123538/148‑1 (MQ=255)

                                          ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                                                                                        <  1:6887/148‑1 (MQ=255)

                                            cACGATATAAATAGCTCCCTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgatg                                                                                                      <  1:713236/148‑1 (MQ=255)

                                             aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATTTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAAtgatga                                                                                                     <  1:964576/148‑1 (MQ=255)

                                             aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATAAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAAtgatga                                                                                                     >  1:891335/1‑148 (MQ=255)

                                             aCGATATAAATAGATCCATTTTCCTTTTATTTTGCTATACGTCTCATCAATACGCCATTTGTACTAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATACTTTTGCACCCAACGGTAGATCCTGGAAtgatga                                                                                                     >  2:695903/1‑148 (MQ=11)

                                              cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCTAAATTTGATATAAAATTGGGGAATATTCTTGAATCCAACGGTAGATCGTTGTATGATGaa                                                                                                    >  1:251995/1‑148 (MQ=11)

                                                atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACg                                                                                                  <  2:1185427/148‑1 (MQ=255)

                                                atacacatcgCTCCATTTTCCTTTTTTTTTGAAACACGTCTCATCAATACCCCATTTGTAATAAGCTTTTTTATGCTTTTTCCTCCAACTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACg                                                                                                  <  1:401286/139‑1 (MQ=11)

                                                      aTAGCTCCATTTTCCTTTTATTTTGATACACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                            <  1:636046/148‑1 (MQ=255)

                                                        aGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTTACTCCAACGGTCGATCGTTGAATGATGAACGTTTAcacc                                                                                          >  2:531210/1‑148 (MQ=11)

                                                        aGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                          >  1:738490/1‑148 (MQ=255)

                                                        aGCTCCATTTTCATTTTATTTTAATATAAGTCTCATCAATACGCCATTTGTAATAAGCTTTTATATACTTTTTCTTCCTAATTTGATAAGTAATTTAGGAATTTTTTTTAACCGAACGTTCGATCGTTGAATTATAAAAGTTTaccca                                                                                          >  2:609591/1‑145 (MQ=2)

                                                         gCTCCGTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                                                                                         <  2:1078175/148‑1 (MQ=11)

                                                          cTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcaccac                                                                                        >  1:1064101/1‑148 (MQ=21)

                                                          cTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAaaccac                                                                                        >  1:162370/1‑148 (MQ=25)

                                                           tCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCCCg                                                                                       >  1:778474/1‑148 (MQ=21)

                                                           tCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                                                                                       >  1:274982/1‑148 (MQ=255)

                                                            ccATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGt                                                                                      >  1:1105112/1‑148 (MQ=21)

                                                            ccATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCAAACGGTAGATCGTTGAATGATGAACGTTTAcacccagt                                                                                      >  2:441095/1‑144 (MQ=11)

                                                             cATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt                                                                                     >  1:568686/1‑148 (MQ=40)

                                                             cATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                                     >  1:929528/1‑148 (MQ=255)

                                                             cATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                                     >  1:655497/1‑148 (MQ=255)

                                                              aTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGtggtgtaaacgttc                                                                                    <  2:284313/148‑14 (MQ=255)

                                                               ttttTCTTTTATTTTGATATACGTCTCATCAATACGCCATGTGTAATAAGGTTTTTTATGCTTTTTCTTCCAAATGTTATAAAAAATTGGGGCATAGTCTTCAACCCAACGGTAGATCGTGGACTGATGCTCCTTTACACGACGTTct                                                                                   <  2:564356/148‑2 (MQ=11)

                                                               ttttCCTTTTATTTTGATATACGTATTATCGTCTCTCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAGGAACGGTTACACCACGTTct                                                                                   <  2:257972/148‑2 (MQ=11)

                                                                tttCCTTTTATTTTTATATACGTCTCATCAATACGCCATCTGTAATAAGCTTTTTTATGCTTTTTCTCCCAAATTTGATATAAAATTGGGGCATATTCTTGAGCCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctc                                                                                  <  2:485490/148‑3 (MQ=11)

                                                                tttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTccc                                                                                  <  1:1001191/148‑1 (MQ=18)

                                                                tttCCTTTTATTTTGATATACGTCTCATCAATCGGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATCTGATATAAGATTGGGGCAGATTCTTGAACCCAACGGTAGATCGGTGAATGATGAAAGTTTACACCAGGtacgc                                                                                  >  2:341156/1‑144 (MQ=11)

                                                                  tCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                                <  2:1032513/148‑1 (MQ=18)

                                                                  tCCTTTTATTTTGATGGACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGTTGAACGTTTACACCACGTTCCCtt                                                                                <  2:405301/148‑1 (MQ=255)

                                                                   ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTGATTCTCCCTTCGAATTTGAAATATAAATGGGGCATATTCTTGAACCCAACGGGAGACCGGTGAATGATGAACGTGTACACCACGTTCCCTTa                                                                               >  1:292996/1‑148 (MQ=11)

                                                                       ttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTTTTTTTTCTTCCAAATTTGATATAAAATTTGGGCATATTGTTGAACCCAACGGTAGACCGTTGAATTATGACCGTTTACACCTCGTTCCCTTAatat                                                                           >  2:457928/1‑148 (MQ=11)

                                                                        tATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAtt                                                                          <  2:138602/148‑1 (MQ=11)

                                                                         aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAttt                                                                         <  2:972489/148‑1 (MQ=255)

                                                                           tttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTAATGCTTTGTCGTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTAAATGATGAACGTTTACACCCCGTTCTCTTAATATTCCa                                                                       >  2:820109/1‑148 (MQ=11)

                                                                            tttatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAg                                                                      <  1:91542/142‑1 (MQ=11)

                                                                                tggaCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGatat                                                                  <  2:368165/145‑1 (MQ=18)

                                                                                  tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatatat                                                                <  1:232519/148‑1 (MQ=255)

                                                                                   aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATc                                                               >  1:947038/1‑148 (MQ=21)

                                                                                   aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAGTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATc                                                               <  1:310645/148‑1 (MQ=11)

                                                                                    cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGACCCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGTTATATcc                                                              >  2:191734/1‑147 (MQ=11)

                                                                                    cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATGTct                                                              >  2:937151/1‑147 (MQ=255)

                                                                                    cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCa                                                              >  1:98569/1‑148 (MQ=255)

                                                                                    cGTCTCATCAAGACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTGACACCACGGTCTCTTAATATTTCAGATATATCa                                                              >  1:848724/1‑148 (MQ=11)

                                                                                     gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATcgc                                                             >  2:588569/1‑146 (MQ=255)

                                                                                       ctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGGTTTATCACgg                                                           >  2:842192/1‑147 (MQ=11)

                                                                                          atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATCTTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                        <  2:506543/148‑1 (MQ=21)

                                                                                          atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                        >  1:1078456/1‑147 (MQ=255)

                                                                                          atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCGCTTAATATTTCAGATATATCACGATag                                                        >  1:822657/1‑147 (MQ=255)

                                                                                           tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagc                                                       >  2:778331/1‑146 (MQ=255)

                                                                                            caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagcc                                                      >  1:61380/1‑145 (MQ=255)

                                                                                              aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                                    <  1:334534/148‑1 (MQ=21)

                                                                                               tACGCCATTTGTAATAATCTTTTTTATTCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                                   <  1:1168862/148‑1 (MQ=255)

                                                                                               tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                                   <  1:866822/148‑1 (MQ=255)

                                                                                               tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                                   >  2:213591/1‑148 (MQ=21)

                                                                                               tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                                   >  1:43758/1‑148 (MQ=40)

                                                                                                aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                  <  2:75956/148‑1 (MQ=255)

                                                                                                aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                  <  1:1194270/148‑1 (MQ=11)

                                                                                                  gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                                <  2:1116240/148‑2 (MQ=11)

                                                                                                   ccATTTGTAATAAGCTTTTTTTTGCTTTTTATTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCATATATATCACGATAACTAAATgca                                               >  2:1009040/1‑146 (MQ=11)

                                                                                                    cATTTGTCATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATAGAAAATTGGGGCATATTCTTGAGCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGcat                                              <  1:184963/148‑1 (MQ=255)

                                                                                                     aTTTGTAATAATCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATGcata                                             <  1:542894/148‑1 (MQ=11)

                                                                                                       ttGTTATAAGCTTTTTTATGCTTTTTCTGCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTCCACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGCATATc                                           <  2:6337/148‑1 (MQ=11)

                                                                                                          taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGCATATCTTa                                        >  2:163260/1‑148 (MQ=14)

                                                                                                                            tttttCTTCCAAATTTGATACAAAATTGGGTCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTa                      <  2:42198/148‑1 (MQ=25)

                                                                                                                              tttCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACa                    <  1:504189/148‑1 (MQ=35)

                                                                                                                                    ccAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGGTAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATa              >  1:796340/1‑148 (MQ=25)

                                                                                                                                              tACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGt    >  2:91211/1‑148 (MQ=34)

CTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTA  >  CP000730/36234‑36525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`