Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 2,532,298 A→T T15T (ACA→ACT)  murN → FemAB family peptidoglycan biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,532,2980AT99.2% 411.3 / NA 126T15T (ACA→ACT) murNFemAB family peptidoglycan biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (38/87);  minor base G (0/1);  total (38/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

TTTCTATGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACGCACA  >  CP000730/2532155‑2532439
                                                                                                                                               |                                                                                                                                             
tttCTATGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTActacaa                                                                                                                                           <  1:1215030/148‑1 (MQ=255)
 ttCTATGTGTATTTGTAATTAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAATTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACaaa                                                                                                                                          <  2:1203648/148‑1 (MQ=255)
 ttCTATGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACaaa                                                                                                                                          <  2:58380/148‑1 (MQ=255)
       gtgtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttt                                                                                                                                    >  1:197574/1‑148 (MQ=255)
       ggttatagtgaaTCAGTTTTATATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACCTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttt                                                                                                                                    <  2:1112159/138‑1 (MQ=255)
        tgtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAGTGCGTTTACTACAAAGCAtttt                                                                                                                                   <  1:930159/148‑1 (MQ=255)
        tgtATTTGTAATCAGTTTTTGAGAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAtttt                                                                                                                                   <  1:264986/148‑1 (MQ=255)
          tatctGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTc                                                                                                                                 >  1:595667/5‑148 (MQ=255)
          tATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTc                                                                                                                                 <  2:1148969/148‑1 (MQ=255)
           aTTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTGCGTATCAGTTCAATGCGTTTACTACAAAGCATTTATca                                                                                                                                >  2:876265/1‑148 (MQ=255)
             ttGTAATCAGTTTTTAATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTcaca                                                                                                                              <  2:731844/148‑1 (MQ=255)
              tGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACAt                                                                                                                             >  1:1051424/1‑148 (MQ=255)
              tGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACAt                                                                                                                             <  1:493783/148‑1 (MQ=255)
              tGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACAt                                                                                                                             >  1:26097/1‑148 (MQ=255)
              tGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAAGGATGGGATAAAATGAATTTTGTAAGGGTGACTTCGGATGAGTTCAATGCGTTTATTACAAAACATTTTTcagtt                                                                                                                             >  2:295858/1‑145 (MQ=255)
                tAATCAGTTTTTGATAAAATAAAAAAATAGCATTATAAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTa                                                                                                                           <  2:468000/148‑1 (MQ=255)
                 aaTTAGTTTTTTATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGAGGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTac                                                                                                                          <  2:1153918/148‑1 (MQ=255)
                 aaTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTac                                                                                                                          <  2:962773/148‑1 (MQ=255)
                 aaTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTac                                                                                                                          <  2:837076/148‑1 (MQ=255)
                 aaTCAATTTTATATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTac                                                                                                                          <  2:396610/148‑1 (MQ=255)
                   tCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGGGAGGGCTGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGAATTTTTCCCATTacac                                                                                                                        >  1:179771/1‑148 (MQ=255)
                   tCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTGTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTGCAAAGCATTTTTCACATTacaa                                                                                                                        >  1:42137/1‑147 (MQ=255)
                    cAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTGTCGTTCGATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTGCACATTacaca                                                                                                                       <  1:850867/148‑1 (MQ=255)
                    cAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacaca                                                                                                                       <  1:605514/148‑1 (MQ=255)
                    cAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacaca                                                                                                                       <  1:1091192/148‑1 (MQ=255)
                    cAGTTTTTGATAAAATAAAAAAATAGCATGATTAATAAATCACAGCAAACTATTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacaca                                                                                                                       <  1:586866/148‑1 (MQ=255)
                     aGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTATAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCGCCTTacccaa                                                                                                                      >  2:163251/1‑147 (MQ=255)
                      gTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTAcacaca                                                                                                                     <  2:1078011/148‑1 (MQ=255)
                      atttttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTAcacaca                                                                                                                     <  2:459258/147‑1 (MQ=255)
                           ttataaaataaaAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATGCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAg                                                                                                                <  1:444116/146‑1 (MQ=255)
                           tGATAAAATAAAAAAATAGCTTTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAg                                                                                                                <  2:362119/148‑1 (MQ=255)
                           tGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAg                                                                                                                <  1:550151/148‑1 (MQ=255)
                               aaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTAt                                                                                                            <  1:894181/148‑1 (MQ=255)
                                aaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTAtt                                                                                                           <  1:554376/148‑1 (MQ=255)
                                aaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACACTACACACAATCAGCTAtt                                                                                                           <  2:764265/148‑1 (MQ=255)
                                   aaaaaaaaTAGCATTATTAATAAAATCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCAt                                                                                                        <  1:710295/147‑1 (MQ=255)
                                    aaaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTCAACACAATCAGCTATTAAtt                                                                                                       >  2:637495/1‑148 (MQ=255)
                                    aaaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACACTCAGCTATTCAtt                                                                                                       >  2:1021871/1‑148 (MQ=255)
                                        aaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACaa                                                                                                   <  2:605556/148‑1 (MQ=255)
                                          aTAGCATTAGTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATc                                                                                                 <  1:389730/148‑1 (MQ=255)
                                             gCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATa                                                                                              <  2:199621/148‑1 (MQ=255)
                                             gCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATa                                                                                              >  1:1076305/1‑148 (MQ=255)
                                                ttattaATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATagag                                                                                           <  1:1164558/148‑1 (MQ=255)
                                                 tattaATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTAGACACAATCAACTATTCATTACAATCATAGAGt                                                                                          <  1:626434/148‑1 (MQ=255)
                                                         aaTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTaa                                                                                  <  2:333024/148‑1 (MQ=255)
                                                          aTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATACAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTTTTCATTACAATCATAGAGTTGATTTaaa                                                                                 <  1:647786/148‑1 (MQ=255)
                                                              aaaaaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGc                                                                             <  2:1051424/148‑1 (MQ=255)
                                                              aaaaaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGc                                                                             <  2:26097/148‑1 (MQ=255)
                                                                aaaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGa                                                                           >  2:89672/1‑148 (MQ=255)
                                                                 caacgcaGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAAAGTTGACATCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAACGGCGAt                                                                          >  2:826368/7‑148 (MQ=255)
                                                                 aaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAt                                                                          >  1:182677/1‑148 (MQ=255)
                                                                       tgTTAAATTCAGAGAGGGATGGGATACAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCAt                                                                    <  1:876265/147‑1 (MQ=255)
                                                                       aGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCAt                                                                    <  1:49843/148‑1 (MQ=255)
                                                                       aGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCAt                                                                    <  1:754791/148‑1 (MQ=255)
                                                                         ttCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCt                                                                  <  1:892801/148‑1 (MQ=255)
                                                                         ttCAAATCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCt                                                                  <  1:147603/148‑1 (MQ=255)
                                                                          tCAATTCAGAGAGGGATGGGATAACATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCtt                                                                 <  2:874678/148‑1 (MQ=255)
                                                                          tCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCtt                                                                 <  1:459869/148‑1 (MQ=255)
                                                                          tCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGGGTATCtt                                                                 <  2:1082944/148‑1 (MQ=255)
                                                                                cAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAggg                                                           <  1:1124443/148‑1 (MQ=255)
                                                                                   agagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGtt                                                        >  1:419470/1‑148 (MQ=255)
                                                                                   agagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGACGATGTGCATCTTGTAGGGGtt                                                        >  1:1097226/1‑148 (MQ=255)
                                                                                     agGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTaa                                                      >  1:993939/1‑148 (MQ=255)
                                                                                                aaaaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATCTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATg                                           >  1:679498/1‑148 (MQ=255)
                                                                                                 aaaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATgg                                          <  1:554033/148‑1 (MQ=255)
                                                                                                   aTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTTTTTTTTTTTTTTATCAGCTAATCATTACAATCATAGAGTTTATTTAAACGGCGGTGTACATCTTGTCGGTTTTAATGATGAAAATGGtt                                        >  2:293015/1‑147 (MQ=255)
                                                                                                   aTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTc                                        <  2:16020/148‑1 (MQ=255)
                                                                                                   aTGAACTTTGTAACGTTGACTTCGGATGAGGTCAATGTGTTTACGACGAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTc                                        <  1:845839/148‑1 (MQ=255)
                                                                                                    tGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCa                                       >  1:1084259/1‑148 (MQ=255)
                                                                                                    tGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCa                                       >  2:154711/1‑148 (MQ=255)
                                                                                                    tGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCa                                       >  2:291500/1‑148 (MQ=255)
                                                                                                           tGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAtt                                >  1:406189/1‑148 (MQ=255)
                                                                                                                tgTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACAGTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTCTAGGGGTTAAAGATGACAATGGTCATGTGATTGCAgg                           <  2:758377/147‑1 (MQ=255)
                                                                                                                 gTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGa                          <  2:406189/148‑1 (MQ=255)
                                                                                                                   tGAGTTCGGATAAGTTAAATGCGTTTACTACAAAGCATTTTTAAATTTAAAAACAAAAAAATATTCATTACAATCATAGAGTTGATTTAAAAGGCGTTTTGCATCTTGTAGGGGTTAAAGATGACAATGGTCATGTGATTGCAGGATg                        <  2:879792/148‑1 (MQ=255)
                                                                                                                   tGACTTCGGATGAGTTGAATGCTTTTACTATAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATg                        <  2:1009728/148‑1 (MQ=255)
                                                                                                                   tGACTTCGGAGTAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATg                        <  1:393030/148‑1 (MQ=255)
                                                                                                                    gtcTTCGGATGTGTTCAGTGCGTGTACTACAAAGTA‑TTGTCGCCTTCCACGAAAGAAGCTATACAGTACAATCTTAGGGATGAATTAAAAAGCGATGAACATCCGCTGATGGATAGAAATTACAATTAGCAAGTGATTGCAGGATGCt                      <  2:358359/146‑1 (MQ=255)
                                                                                                                    gACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATATAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGc                       <  1:1105766/148‑1 (MQ=255)
                                                                                                                     aCTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCt                      >  2:87537/1‑148 (MQ=255)
                                                                                                                      cTTCGGAGTAGTTCAATGCGTTTACTACAAAGCCTTTTTCACATTACACCCAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCtt                     <  1:128968/148‑1 (MQ=255)
                                                                                                                       ttCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTa                    >  1:660402/1‑148 (MQ=255)
                                                                                                                         cGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTAtt                  >  1:1173120/1‑148 (MQ=255)
                                                                                                                           gATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGa                >  1:221350/1‑148 (MQ=255)
                                                                                                                            aTGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGAc               >  1:368303/1‑148 (MQ=255)
                                                                                                                             tGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTGGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACa              >  1:571787/1‑148 (MQ=255)
                                                                                                                              gAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAg             <  1:489344/148‑1 (MQ=255)
                                                                                                                              gAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAg             <  1:243166/148‑1 (MQ=255)
                                                                                                                                 ttCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACGATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAg          >  1:985785/1‑148 (MQ=255)
                                                                                                                                 ttCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAg          >  1:122107/1‑148 (MQ=255)
                                                                                                                                 ttCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAg          >  1:728250/1‑148 (MQ=255)
                                                                                                                                     aTGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgcacg      <  2:368303/148‑1 (MQ=255)
                                                                                                                                     aTGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgcacg      <  1:1110667/148‑1 (MQ=255)
                                                                                                                                      tGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGCGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgcacgc     <  2:896279/148‑1 (MQ=255)
                                                                                                                                      tGCGGTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgcacgc     <  2:595667/148‑1 (MQ=255)
                                                                                                                                       gCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgcacgca    <  1:719051/148‑1 (MQ=255)
                                                                                                                                       gCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTGAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgcacgca    <  1:1178227/148‑1 (MQ=255)
                                                                                                                                         gTTTACTACAGAGCATCTTTCACATTACACACAATCAGCGATCCATTACAATCATAGAGTTGATTTAAAAGTCGATTTGTATCTCGATGGGGTTAAAGATGTCAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACTcaca  <  2:144560/148‑1 (MQ=255)
                                                                                                                                         gTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACGcaca  <  2:221350/148‑1 (MQ=255)
                                                                                                                                         gTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACGcaca  <  2:792357/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                             
TTTCTATGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACGCACA  >  CP000730/2532155‑2532439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑