Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 202,469 A→T 10.0% Q186L (CAA→CTA)  yweA → MFS family major facilitator transporter, multidrug cation symporter or antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730202,4690AT10.0% 114.1 / 3.9 40Q186L (CAA→CTA) yweAMFS family major facilitator transporter, multidrug cation symporter or antiporter
Reads supporting (aligned to +/- strand):  ref base A (31/5);  new base T (2/2);  total (33/7)
Fisher's exact test for biased strand distribution p-value = 1.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

TATTGGATGACAAACTTATCGGTAGCTATTGGGGCATTGTTAGGTGGCTTGATGTATGGCTACAGTATGTTACTACTTTTCTTAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCAAAAATTACAAATAATGAATATATTTCGCAGTTATAAATTAGTTTTGAAAGACCGTAATTATATGTTATTGATTTCGGGGTT  >  CP000730/202330‑202604
                                                                                                                                           |                                                                                                                                       
tATTGGATGACAAACTTATCGGTAGCTATTGGGGCATTGTTAGGTGGCTTGATGTATGGCTACAGTATGTTACTACTTTTCTTAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTATTATATTTGGTTACCTCaa                                                                                                                                        <  1:245088/141‑1 (MQ=255)
    ggATGACAAACTTATCGGTAGCTATTGGGGCATTGTTAGGAGGCTTGCTGTATGGCTACAGTATGTTACTACTTTTCTTAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTCATATATTTGGTTACCTCAAGAcc                                                                                                                                    >  2:300130/1‑141 (MQ=255)
      aTGACAAACTTATCGGTAGCTATTGGGGCATTGTTAGGTGGCTTGAGGTATGGCTACAGTATGTTACTACTTTTCTTAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCAAGACCGa                                                                                                                                  >  1:92621/1‑141 (MQ=255)
       tGACAAACTTATCGGTAGCTATTGGGGCATTGTTAGGTGGCTTGATGTATGGCTACAGTATGTTACTACTTTTCTTAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCTAGACCGaa                                                                                                                                 >  2:122287/1‑141 (MQ=255)
       tGACAAACTTATCGGTAGCTATTGGGGCATTGTTAGGTGGCTTGATGTATGGCTACAGTATGTTACTACTTTTCTTAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCTAGACCGaa                                                                                                                                 >  2:122294/1‑141 (MQ=255)
         aCAAACTTATCGGTAGCTATTGGGGCATTGTTAGGTTGCTTGATGTATGGCTACAGTATGTTACTACTTTTCTTAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCAAGACCGAAAt                                                                                                                               <  2:331929/141‑1 (MQ=255)
              cTTATCGGTAGCTATTGGGGCATTGTTAGGTGGCTTGATGTATGGCTACAGTATGTTACTACTTTTCTTAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCAAGACCGAAATCAAGt                                                                                                                          >  1:284755/1‑141 (MQ=255)
                     gTAGCTATTGGGGCATTGTTAGGTGGCTTGATGTATGGCTACAGTATGTTACTACTTTTCTTAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCAAGACCGAAATCAAGTAAAGCaa                                                                                                                   >  2:410602/1‑141 (MQ=255)
                               gggCATTGTTAGGTGGCTTGATGTATGGCTACAGTATGTTACTACTTTTCTTAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCAAGGCGGAAATCAAGTAAAGAAAAGtggtgaga                                                                                                         >  1:160193/1‑139 (MQ=255)
                                ggCATTGTTAGGTGGCTTGATGTATGGCTACAGTATGTTACTACTTTTCTTAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCTAGACCGAAATCAAGTAAAGCAAAGTGATGACaa                                                                                                        <  1:122294/141‑1 (MQ=255)
                                ggCATTGTTAGGTGGCTTGATGTATGGCTACAGTATGTTACTACTTTTCTTAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCTAGACCGAAATCAAGTAAAGCAAAGTGATGACaa                                                                                                        <  1:122287/141‑1 (MQ=255)
                                     tGTTAGGTGGCTTGATGTATGGCTACAGTATGTTACTACTTTTCTTAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAgga                                                                                                   >  2:129688/1‑140 (MQ=255)
                                       ttAGGTGGCTTGATGTATGGCTACAGTATGTTACTACTTTTCTTAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCAt                                                                                                 >  2:419025/1‑141 (MQ=255)
                                        tAGGTGGCTTGATGTATGGCTACAGTATGTTACTACTTTTCTTAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTAGCTCAAGACCGAAATCAAGTAAAGCAAAGTGATTACCAGGGGCATg                                                                                                >  1:156446/1‑141 (MQ=255)
                                        tAGGTGGCTTGATGTATGGCTACAGTATGTTACTACTTTTCTTAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATg                                                                                                >  1:176758/1‑141 (MQ=255)
                                                      tATGGCTACAGTATGTTACTACTTTTCTTAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCaa                                                                                  >  1:290886/1‑141 (MQ=255)
                                                       aTGGCTGCAGTATGTTACTACTTTTCTTAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCaaa                                                                                 >  1:100926/1‑141 (MQ=255)
                                                                 tATGTTACTACTTTTCTTAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATGTATTTGGTTACCTCAAGTCCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCGTGCAAGTCGTTCTCAAAAATTACaaaa                                                                       >  2:268397/1‑140 (MQ=255)
                                                                   tGTTACTACTTTTCTTAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCAAAAATTACaaaaaa                                                                     >  2:92365/1‑141 (MQ=255)
                                                                             tttCTTAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGTTGACCTTAATACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCAAAAATTACAAATAATGAATATAtt                                                           >  2:165867/1‑141 (MQ=255)
                                                                                 ttAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCAAAAATTACAAATAATGAATATATTTCGc                                                       <  1:55607/141‑1 (MQ=255)
                                                                                 ttAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCAAAAATTACAAATAATGAATATATTTCGc                                                       >  1:256753/1‑141 (MQ=255)
                                                                                 ttAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCAAAAATTACAAATAATGAATATATTTCGc                                                       >  2:262842/1‑141 (MQ=255)
                                                                                     tAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCAAAAATTACAAATAATGAATATATTTCGCAGtt                                                   >  1:250373/1‑141 (MQ=255)
                                                                                     tAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTGGGTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCAAAAATTACAAATAATGAATATATTTCGCAGtt                                                   >  1:262058/1‑141 (MQ=255)
                                                                                         agcTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTCCCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCAAAAATTACAAATAATGAATATATTTCGCAGTTATaa                                               <  1:351505/141‑1 (MQ=255)
                                                                                         aacTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCAAAAATTACAAATAATGAATATATTTCGCAGTTATaa                                               >  2:283306/3‑141 (MQ=255)
                                                                                          gcTTGTATATTTTTTTTTTTTTTTTTTATTTTATATATTTGGTTACCTTAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCAAAAATTACAAATAATGTTTATTTTTCGCAGTTATaaa                                              >  2:169910/1‑141 (MQ=255)
                                                                                          gcTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCAAAAATTACAAATAATGAATATATTTCGCAGTTATaaa                                              >  2:69836/1‑141 (MQ=255)
                                                                                          gcTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCAAAAATTACAAATAATGAATATATTTCGCAGTTATaaa                                              >  2:364836/1‑141 (MQ=255)
                                                                                                atatTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCAAAAATTACAAATAATGAATATATTTCGCAGTTATAAATTAGtt                                        >  2:80259/1‑141 (MQ=255)
                                                                                                   tttttAATTGTACTCTTTATTTTATATATTTGGTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCAAAAATTACAAATAATGAATATATTTCGCAGTTATAAATTAGTTTTg                                     <  2:116711/141‑1 (MQ=255)
                                                                                                         ttttttttctTTATTTTATATATTTGGTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATTCAAGTCGTTATCTAAAATTTCAAATAATGAATATATTTCGCAGTTATAAATTAGTTTAGAAAAAc                               >  2:65666/8‑141 (MQ=255)
                                                                                                             tACTCTTTATTTTATATATTTGGTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCAAAAATTACAAATAATGAATATATTTCGCAGTTATAAAATAGTTTTCAAAGACCGTa                           >  2:150556/1‑141 (MQ=255)
                                                                                                              aCTCTTTATTTTATATATTTGGTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCAAAAATTACAAATAATGAATATATTTCGCAGTTATAAATTAGTTTTGAAAGAACGtga                          >  1:379520/1‑139 (MQ=255)
                                                                                                                            atatTTGGTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCAAAAATTACAAATAATGAATATATTTCGCAGTTCTAAAATAGTTTTGAAAGACCGTAATTATAAGTTAttaa            >  2:410668/1‑139 (MQ=255)
                                                                                                                               tttGGTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCAAAAATTACAAATAATGAATATATTTCGCAGTTATAAATTAGTTTTGAAAGACCGTAATTATATGTTATTGAttt         >  1:414458/1‑141 (MQ=255)
                                                                                                                                  ggTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATACAAGTAGTTATCAAAAAATACAAATAATGAATATATATCACAGTTATAAATTAGTTTTGAACGACCGTACTTATATGTTATTGATTTctg      >  2:199933/1‑139 (MQ=255)
                                                                                                                                      aCCTCAAGACCGAAATCAAATAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCAAAAATTACAAATAATGAATATATTTCGCAGTTATAAATTAGTTTTGAAAGACCGTAATTATATGTTATTGATTTCGGGGtt  >  1:245065/1‑141 (MQ=255)
                                                                                                                                        cTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCAAAAATTACa                                                                          >  2:450675/1‑67 (MQ=255)
                                                                                                                                        cTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCAAAAATTACa                                                                          <  1:450675/67‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                       
TATTGGATGACAAACTTATCGGTAGCTATTGGGGCATTGTTAGGTGGCTTGATGTATGGCTACAGTATGTTACTACTTTTCTTAATAGCAGCTTGTATATTTTTAATTGTACTCTTTATTTTATATATTTGGTTACCTCAAGACCGAAATCAAGTAAAGCAAAGTGATGACAAGAGGCATGCAAGTCGTTATCAAAAATTACAAATAATGAATATATTTCGCAGTTATAAATTAGTTTTGAAAGACCGTAATTATATGTTATTGATTTCGGGGTT  >  CP000730/202330‑202604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑