Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,761,889	C→A	100%	D388Y (GAT→TAT)	folC ←	tetrahydrofolate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,761,889	0	C	A	100.0%	163.8 / NA	50	D388Y (GAT→TAT)	folC	tetrahydrofolate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (29/21);  total (29/21)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTCTTCATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGTTTTC  >  CP000730/1761752‑1762026                                                                                                                                          |                                                                                                                                          ttcttcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATatac                                                                                                                                        >  1:370132/1‑141 (MQ=255)        tGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGt                                                                                                                                 <  2:303814/141‑1 (MQ=255)                  agttttagttttATAATGCTTCCACGTCTAATTTTGATTTAACTTCACTGATAAAATACAGACTACCGGTAATTACGAATGGATCACCTGGATAATTTTTTATAAAGTCAACGTAGTCATATACTAATTGTATTTcatcat                                                                                                                       <  2:268002/141‑1 (MQ=255)                   gttttagttttaTAAAGCTTCAAAGTACAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCAtt                                                                                                                      <  1:262826/141‑1 (MQ=255)                      ttagttttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAGTTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTc                                                                                                                   >  1:160154/1‑141 (MQ=255)                             tataATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAAtacta                                                                                                            >  2:460086/1‑141 (MQ=255)                             tataATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAAtacta                                                                                                            >  1:391794/1‑141 (MQ=255)                                   gCTTCAAAGTCTAATTTTGATGTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACa                                                                                                      >  2:169688/1‑141 (MQ=255)                                     ttacaaGTATAATGTTGATCTAACTTCACTTATGAAATACAGGTTCCCGGTTATTACTAATGTATCACCTTGATAATTCTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAAt                                                                                                    <  2:263462/137‑1 (MQ=255)                                      tCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAAtt                                                                                                   >  1:121173/1‑141 (MQ=255)                                       cAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAAttt                                                                                                  >  1:399291/1‑141 (MQ=255)                                          aggcTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATttctt                                                                                               >  2:232615/4‑141 (MQ=255)                                            tCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTtcttct                                                                                             >  1:429852/1‑141 (MQ=255)                                             cTAATTTTGATTTAACTTCACTTATGAAATACAGACAACCGGGAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTcttctt                                                                                            >  2:2481/1‑141 (MQ=255)                                                aTTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGc                                                                                         >  2:68608/1‑141 (MQ=255)                                                  tttGATTTAACTTCCCTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGACAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGt                                                                                       <  2:233370/141‑1 (MQ=255)                                                  tttGATTTAACTTCACTTATGAAATAGAGACTTCCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGt                                                                                       <  1:421696/141‑1 (MQ=255)                                                  tttGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGt                                                                                       <  1:415838/141‑1 (MQ=255)                                                   ttGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACATAGGCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTa                                                                                      <  1:159894/141‑1 (MQ=255)                                                              tCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCGTTTTCAATACTACCTACCATTTCTTTTTTGCGTAAAGCTTTCGGa                                                                           >  1:54528/1‑141 (MQ=255)                                                                   tATTAAATACCGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATc                                                                      <  1:42825/141‑1 (MQ=255)                                                                   tATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAAta                                                                      >  1:100660/1‑140 (MQ=255)                                                                    aTGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCa                                                                     <  2:441992/141‑1 (MQ=255)                                                                     tGAAATACAGACTACCGGTAATTCCTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTCTTTCATCATTTTCAATGCTACCTACCATTTCTTCTTTGCGTAACGCTTCCTGaaagatca                                                                    >  2:427783/1‑136 (MQ=255)                                                                     tGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCaa                                                                    <  2:121173/141‑1 (MQ=255)                                                                      taaaTACAGACCACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCaaa                                                                   <  1:114440/140‑1 (MQ=255)                                                                           aCAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAg                                                              >  2:264387/1‑141 (MQ=255)                                                                            cAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGt                                                             <  1:134146/141‑1 (MQ=255)                                                                              gACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTg                                                           >  1:35817/1‑141 (MQ=255)                                                                              gACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATCGCTTCTTTGCGTAAGCCTTTAGGAAAATCAAATTCAGttt                                                           >  2:111499/1‑140 (MQ=255)                                                                               aCTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGc                                                          >  2:442898/1‑141 (MQ=255)                                                                                 tACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCAt                                                        <  1:452641/141‑1 (MQ=255)                                                                                 tACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCAt                                                        <  1:452617/141‑1 (MQ=255)                                                                                  aCCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATa                                                       <  1:68608/141‑1 (MQ=255)                                                                                  aCCGGGAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATa                                                       <  2:164954/141‑1 (MQ=255)                                                                                    cGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATaaa                                                     >  1:246168/1‑141 (MQ=255)                                                                                            aCTAATGTATCACCTTGATAATTTTTTATAAAGTCAACGTAGTCATATACTAATTGCATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATgc                                             >  1:128227/1‑141 (MQ=255)                                                                                             cTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATgcg                                            >  2:1159/1‑141 (MQ=255)                                                                                             cTAATGTATCACCTTGAGAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGTGTTTCAAATTCAGTTGCATAAAACGTATgcg                                            <  1:17904/141‑1 (MQ=255)                                                                                                     tCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCCTAAAGCCTCCGCATAATCAAATTCAGTTGCTTAAAACGTATGCGCAATaaaa                                    >  2:393487/1‑141 (MQ=255)                                                                                                          ttGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAACCTTaa                               >  1:150698/1‑141 (MQ=255)                                                                                                           tGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGAGCAATTAAACTTaaa                              >  2:380242/1‑141 (MQ=255)                                                                                                              ttaTTTTTTATAAATTCAACGTAGTCATATCCTGATTGTCTTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAACCTTAAATGt                           <  2:320277/139‑1 (MQ=255)                                                                                                              tAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACTTATGCGCAATTAAACCTAAAttt                           >  2:118445/1‑139 (MQ=255)                                                                                                                aTTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGttt                         >  2:382569/1‑141 (MQ=255)                                                                                                                       ataAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCAtc                  <  2:401283/141‑1 (MQ=255)                                                                                                                            ttCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTCTGCGCAATTAAACTTAAATGTTTGACCATCTCGtt             >  2:323584/1‑141 (MQ=255)                                                                                                                               aaCGTAGTCATATACTGATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAAt          <  2:217871/141‑1 (MQ=255)                                                                                                                                     gTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGttt    >  1:368128/1‑141 (MQ=255)                                                                                                                                     gTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCATTAATCGGttt    >  1:368180/1‑141 (MQ=255)                                                                                                                                       cATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAACTCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTACTCGGTTTTc  >  2:179238/1‑141 (MQ=255)                                                                                                                                          |                                                                                                                                          TTCTTCATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGGTTTTC  >  CP000730/1761752‑1762026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑